SSGCID: Target Page

MythA.19493.a

Dephospho-CoA kinase CoaE; protein folding accessory domain-containing protein (EC 2.7.1.24)

CENTER ID:	MythA.19493.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.19493.a.B1.GE40760	full length		1	405
MythA.19493.a.B2.GE40761	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.19493.a.B2.PW38006	nterm domain		1	204

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.407
UniProt:	G7CBS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIRIGLTGGI GAGKSTVAAT FAECGGIIVD GDVIAREVVQ PGTEGLAKLV ETFGEDILLP DGSLDRPALA AKAFADDEQR AKLNAIVHPL VGRRREEIIA AVPDDAVVVE DIPLLVETGM APTFPLVVVV YADAEVRVKR LVEIRGMSEQ DARARIAAQA TDEQRAAIAD VLLDNSGTQQ EIVDKARALW EQRVLPLAEN IRTGRCVTPA LELVAPQPHW RADAARIIAR IQMACGAKAL RVDHIGSTAV DGLSAKDVID IQVTVGSLDA ADEIAEPLAR VGYPRRAEIT SDVAKPDARS TVAEFDHSAD ASLWRKRLHG AADPGRPANI HIRVDGWPNQ QFALLFADWL NAEPAVRDEY LSVKQRAVST GDLDAYTEAK EPWFAEAYRR AWDWADRTGW RPSPR

NT Sequence

atgattcgga tcggtctgac cggtggtatc ggcgccggca agtccaccgt cgccgcgacg ttcgcggaat gcgggggcat catcgtggac ggcgacgtca tcgcccggga ggtggtccag cccggaaccg aggggctggc caaactggtc gagacgttcg gcgaggacat cctgctgccc gacggctcgc tggaccggcc cgccctggcg gccaaggcct tcgccgacga cgagcagcgc gccaagctga acgcgatcgt gcacccgctg gtgggtaggc gccgggagga gatcatcgcg gcggtgcccg acgacgcggt ggtggtcgag gacatcccgc tgctggtgga aaccggtatg gcgccgacgt tcccgctggt cgtggtggtc tacgcggacg ccgaggtccg ggtgaagcgg ctggtggaga tccgcgggat gagcgaacag gacgcccggg cccgcatcgc cgcgcaggcc accgatgagc agcgcgccgc gatcgccgat gtgctgctgg acaactccgg aacccagcag gagatcgtcg acaaggcgcg cgcgttgtgg gaacagcggg tgctgccgtt ggcggagaac atccgcaccg gtcggtgcgt gacacccgcc ctcgaactgg ttgcgcccca gccgcactgg cgcgccgacg ctgcgcgcat catcgcacgg atccagatgg cctgcggcgc gaaggcgctc cgcgtcgacc acatcggatc caccgccgtg gacggtcttt ccgccaagga cgtcatcgac atccaggtga cggtcggctc cctcgacgcc gccgacgaga tcgccgagcc gctggcccgg gtgggctatc cacgacgcgc ggagatcacc tcagacgtcg ccaaacccga cgcccgcagc accgtcgccg agttcgacca cagcgccgat gcgtcgttgt ggcggaagcg gttgcacggc gccgccgacc cgggacgccc ggccaacatc cacatccggg tggacggctg gcccaatcag cagttcgcgc tgctgttcgc cgactggctc aacgccgaac cggccgtccg ggacgagtac ctgtcggtca aacagcgggc ggtctccacc ggcgacctcg acgcgtacac cgaggccaag gaaccgtggt tcgccgaggc ctatcggcgg gcctgggact gggccgaccg caccggctgg cggccgtcgc cccgctga

Details for MythA.19493.a.B1.GE40760

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	45kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMI RIGLTGGIGA GKSTVAATFA ECGGIIVDGD VIAREVVQPG TEGLAKLVET FGEDILLPDG SLDRPALAAK AFADDEQRAK LNAIVHPLVG RRREEIIAAV PDDAVVVEDI PLLVETGMAP TFPLVVVVYA DAEVRVKRLV EIRGMSEQDA RARIAAQATD EQRAAIADVL LDNSGTQQEI VDKARALWEQ RVLPLAENIR TGRCVTPALE LVAPQPHWRA DAARIIARIQ MACGAKALRV DHIGSTAVDG LSAKDVIDIQ VTVGSLDAAD EIAEPLARVG YPRRAEITSD VAKPDARSTV AEFDHSADAS LWRKRLHGAA DPGRPANIHI RVDGWPNQQF ALLFADWLNA EPAVRDEYLS VKQRAVSTGD LDAYTEAKEP WFAEAYRRAW DWADR

Validated NT Sequence

ggtgcggtcg gcccagtccc aggcccgccg ataggcctcg gcgaaccacg gttccttggc ctcggtgtac gcgtcgaggt cgccggtgga gaccgcccgc tgtttgaccg acaggtactc gtcccggacg gccggttcgg cgttgagcca gtcggcgaac agcagcgcga actgctgatt gggccagccg tccacccgga tgtggatgtt ggccgggcgt cccgggtcgg cggcgccgtg caaccgcttc cgccacaacg acgcatcggc gctgtggtcg aactcggcga cggtgctgcg ggcgtcgggt ttggcgacgt ctgaggtgat ctccgcgcgt cgtggatagc ccacccgggc cagcggctcg gcgatctcgt cggcggcgtc gagggagccg accgtcacct ggatgtcgat gacgtccttg gcggaaagac cgtccacggc ggtggatccg atgtggtcga cgcggagcgc cttcgcgccg caggccatct ggatccgtgc gatgatgcgc gcagcgtcgg cgcgccagtg cggctggggc gcaaccagtt cgagggcggg tgtcacgcac cgaccggtgc ggatgttctc cgccaacggc agcacccgct gttcccacaa cgcgcgcgcc ttgtcgacga tctcctgctg ggttccggag ttgtccagca gcacatcggc gatcgcggcg cgctgctcat cggtggcctg cgcggcgatg cgggcccggg cgtcctgttc gctcatcccg cggatctcca ccagccgctt cacccggacc tcggcgtccg cgtagaccac cacgaccagc gggaacgtcg gcgccatacc ggtttccacc agcagcggga tgtcctcgac caccaccgcg tcgtcgggca ccgccgcgat gatctcctcc cggcgcctac ccaccagcgg gtgcacgatc gcgttcagct tggcgcgctg ctcgtcgtcg gcgaaggcct tggccgccag ggcgggccgg tccagcgagc cgtcgggcag caggatgtcc tcgccgaacg tctcgaccag tttggccagc ccctcggttc cgggctggac cacctcccgg gcgatgacgt cgccgtccac gatgatgccc ccgcattccg cgaacgtcgc ggcgacggtg gacttgccgg cgccgatacc accggtcaga ccgatccgaa tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc ggatcggtct gaccggtggt atcggcgccg gcaagtccac cgtcgccgcg acgttcgcgg aatgcggggg catcatcgtg gacggcgacg tcatcgcccg ggaggtggtc cagcccggaa ccgaggggct ggccaaactg gtcgagacgt tcggcgagga catcctgctg cccgacggct cgctggaccg gcccgccctg gcggccaagg ccttcgccga cgacgagcag cgcgccaagc tgaacgcgat cgtgcacccg ctggtgggta ggcgccggga ggagatcatc gcggcggtgc ccgacgacgc ggtggtggtc gaggacatcc cgctgctggt ggaaaccggt atggcgccga cgttcccgct ggtcgtggtg gtctacgcgg acgccgaggt ccgggtgaag cggctggtgg agatccgcgg gatgagcgaa caggacgccc gggcccgcat cgccgcgcag gccaccgatg agcagcgcgc cgcgatcgcc gatgtgctgc tggacaactc cggaacccag caggagatcg tcgacaaggc gcgcgcgttg tgggaacagc gggtgctgcc gttggcggag aacatccgca ccggtcggtg cgtgacaccc gccctcgaac tggttgcgcc ccagccgcac tggcgcgccg acgctgcgcg catcatcgca cggatccaga tggcctgcgg cgcgaaggcg ctccgcgtcg accacatcgg atccaccgcc gtggacggtc tttccgccaa ggacgtcatc gacatccagg tgacggtcgg ctccctcgac gccgccgacg agatcgccga gccgctggcc cgggtgggct atccacgacg cgcggagatc acctcagacg tcgccaaacc cgacgcccgc agcaccgtcg ccgagttcga ccacagcgcc gatgcgtcgt tgtggcggaa gcggttgcac ggcgccgccg acccgggacg cccggccaac atccacatcc gggtggacgg ctggcccaat cagcagttcg cgctgctgtt cgccgactgg ctcaacgccg aaccggccgt ccgggacgag tacctgtcgg tcaaacagcg ggcggtctcc accggcgacc tcgacgcgta caccgaggcc aaggaaccgt ggttcgccga ggcctatcgg cgggcctggg actgggccga ccgcaccggc tggcggccgt cgccccgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for MythA.19493.a.B2.GE40761

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

atcttactca cttaaccggt gcggatgttc tccgccaacg gcagcacccg ctgttcccac aacgcgcgcg ccttgtcgac gatctcctgc tgggttccgg agttgtccag cagcacatcg gcgatcgcgg cgcgctgctc atcggtggcc tgcgcggcga tgcgggcccg ggcgtcctgt tcgctcatcc cgcggatctc caccagccgc ttcacccgga cctcggcgtc cgcgtagacc accacgacca gcgggaacgt cggcgccata ccggtttcca ccagcagcgg gatgtcctcg accaccaccg cgtcgtcggg caccgccgcg atgatctcct cccggcgcct acccaccagc gggtgcacga tcgcgttcag cttggcgcgc tgctcgtcgt cggcgaaggc cttggccgcc agggcgggcc ggtccagcga gccgtcgggc agcaggatgt cctcgccgaa cgtctcgacc agtttggcca gcccctcggt tccgggctgg accacctccc gggcgatgac gtcgccgtcc acgatgatgc ccccgcattc cgcgaacgtc gcggcgacgg tggacttgcc ggcgccgata ccaccggtca gaccgatccg aatcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc ggatcggtct gaccggtggt atcggcgccg gcaagtccac cgtcgccgcg acgttcgcgg aatgcggggg catcatcgtg gacggcgacg tcatcgcccg ggaggtggtc cagcccggaa ccgaggggct ggccaaactg gtcgagacgt tcggcgagga catcctgctg cccgacggct cgctggaccg gcccgccctg gcggccaagg ccttcgccga cgacgagcag cgcgccaagc tgaacgcgat cgtgcacccg ctggtgggta ggcgccggga ggagatcatc gcggcggtgc ccgacgacgc ggtggtggtc gaggacatcc cgctgctggt ggaaaccggt atggcgccga cgttcccgct ggtcgtggtg gtctacgcgg acgccgaggt ccgggtgaag cggctggtgg agatccgcgg gatgagcgaa caggacgccc gggcccgcat cgccgcgcag gccaccgatg agcagcgcgc cgcgatcgcc gatgtgctgc tggacaactc cggaacccag caggagatcg tcgacaaggc gcgcgcgttg tgggaacagc gggtgctgcc gttggcggag aacatccgca ccggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MythA.19493.a.B2.PW38006

PURIFICATION DATe:	1/3/2017
CONCENTRATION:	15.6mg/ml
OBSERVED MW:	26kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTC GGATCGGTCT GACCGGTGGT ATCGGCGCCG GCAAGTCCAC CGTCGCCGCG ACGTTCGCGG AATGCGGGGG CATCATCGTG GACGGCGACG TCATCGCCCG GGAGGTGGTC CAGCCCGGAA CCGAGGGGCT GGCCAAACTG GTCGAGACGT TCGGCGAGGA CATCCTGCTG CCCGACGGCT CGCTGGACCG GCCCGCCCTG GCGGCCAAGG CCTTCGCCGA CGACGAGCAG CGCGCCAAGC TGAACGCGAT CGTGCACCCG CTGGTGGGTA GGCGCCGGGA GGAGATCATC GCGGCGGTGC CCGACGACGC GGTGGTGGTC GAGGACATCC CGCTGCTGGT GGAAACCGGT ATGGCGCCGA CGTTCCCGCT GGTCGTGGTG GTCTACGCGG ACGCCGAGGT CCGGGTGAAG CGGCTGGTGG AGATCCGCGG GATGAGCGAA CAGGACGCCC GGGCCCGCAT CGCCGCGCAG GCCACCGATG AGCAGCGCGC CGCGATCGCC GATGTGCTGC TGGACAACTC CGGAACCCAG CAGGAGATCG TCGACAAGGC GCGCGCGTTG TGGGAACAGC GGGTGCTGCC GTTGGCGGAG AACATCCGCA CCGGTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT