MythA.19275.a

Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)

CENTER ID: MythA.19275.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.19275.a.B2.GE40364 ordered domain 80 759
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.4248
UniProt: G7CMQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEAQAQSRA DRVAGDGVTA PTGADQRGAD QRGADQRGAD QTGADQTDRT GDERWDGSHT APEAPPAATA PAIDDALPED RYLNRELSWL DFNARVLALA ADTSLPLLER AKFLAIFASN LDEFYMVRVA GLKRRHETGL SVRSADGLSP LEQLRRISER TQQLATRHAD VFLNSVRPAL AEEGIVIVTW AELGEAERNK LSTYFHEQVF PVLTPLAVDP AHPFPFVSGL SLNLAIMVKR PEDGGQHFAR VKVPDNVDRF VELDSRPGAD GLIRFLPMEE LIAAFLPVLF PGLEIVEHHA FRITRNADFE VEEDRDEDLL QALERELARR RFGSPVRLEV SDDMTENMLE LLLRELDVHP DDVVEVPGLL DLSSLWQIYD VDRPALKDPP FVPATPPAFG ERETPKSIFA TLRDGDVLVH HPYDSFSTTV QRFIEQAAAD PNVLAIKQTL YRTSGDSPIV NALIDAAEAG KQVVALVEIK ARFDEQANIK WARTLEQAGV HVVYGIIGLK THCKCALVVR REGSVIRRYC HIGTGNYNPK TARLYEDIGL LTAAPDIGAD LTDLFNSLTG YSRKESYRNL LVAPYGVRKG LIDRIEGEIA AKREGADAHI RLKANALVDE QIIDALYRAS LAGVGVEVVV RGICALRPGV PGLSENIRVR SILGRFLEHS RILHFRAQNE FWIGSADMMH RNLDRRVEVM AQVKDPRLTA QLNDILDSAL DPATRCWELG PDGRWTPSPR EGETVRDHQV SMMKRRRYH
NT Sequence
gtgagcgaag cacaggccca gtcccgagcc gaccgtgtgg ccggggacgg cgtcaccgcg ccgaccgggg ccgatcagag gggggccgac cagagggggg ccgaccagag gggggccgac cagacggggg ccgatcagac cgaccggacc ggcgacgagc ggtgggacgg gtcgcacacc gccccggagg cgccgccggc cgccacggca ccggcgatcg acgatgcgct gcccgaggac cgctacctca accgggaact cagctggctg gatttcaacg cccgggtgct ggcgctggcg gccgacacct cgctgcccct gctggagcgg gcgaagttcc tggcgatctt cgcctccaac ctcgacgagt tctacatggt gcgggtggcc gggctgaaac gccgccacga gaccggcctg tcggtccggt cggccgacgg tctgtcgccc ctcgagcagt tgcgtcgcat cagtgagcgc acccagcagc tcgcgacccg ccacgccgac gtgttcctca actcggtgcg gccggcgctg gccgaagagg gcatcgtgat cgtcacgtgg gccgaactcg gtgaggccga acgcaacaag ttgtccacct acttccacga gcaggtcttc ccggtcctga ccccgctggc ggtcgatccg gcccacccgt tcccgttcgt cagcggcctg agcctcaacc tcgcgatcat ggttaaacgc cccgaggacg gcggccagca cttcgcgcgg gtcaaggtgc ccgacaacgt cgatcggttc gtcgaactcg acagccggcc cggcgccgac ggtctgatcc gtttcctgcc gatggaggaa ctgatcgcgg cgttcctgcc ggtgctgttc cccggcctgg agatcgtcga acaccacgcc ttccggatca cccgcaacgc ggacttcgag gtcgaggagg accgcgacga ggacctgttg caggcactgg aacgcgaact ggcccggcgc cgcttcggtt ctccggtgcg cctcgaggtc tccgacgaca tgaccgagaa catgctcgaa ctgctcctgc gggaactcga cgtccatccc gacgatgtgg tcgaggtacc ggggctgctc gacctgtcgt cgctgtggca gatctacgac gtggaccggc cggcgctgaa ggatccgccg ttcgtgcccg ccaccccacc ggctttcggg gaacgcgaga caccgaagag catcttcgcc acgctgcgcg acggtgacgt gctggtgcac cacccctacg actcgttctc caccaccgtc cagcgattca tcgagcaggc cgccgccgac ccgaacgtgt tggcgatcaa gcagaccctg taccgcacct cgggcgactc gccgatcgtc aacgccctga tcgacgccgc cgaggccggc aagcaggtgg tggcgctggt ggagatcaag gcccgcttcg acgagcaggc caacatcaag tgggcccgca ccctcgagca ggccggcgtc catgtggtgt acgggatcat cggcttgaag acgcactgca agtgcgccct ggtggttcgg cgggaaggat cggtgatccg gcgctactgc cacatcggca ccggcaacta caacccgaaa accgcacgcc tctacgaaga catcggactg ctcaccgccg ccccggacat cggcgccgat ctgaccgatc tgttcaactc gctcaccggg tactcccgca aggagtccta ccgcaacctg ctggtggcgc cgtacggcgt ccgcaaggga ctgatcgacc gcatcgaggg tgagatcgcc gccaaacgcg agggcgccga cgcccacatc cggttgaagg ccaatgccct ggtcgacgaa cagatcatcg acgcgctgta ccgcgcctcg ctcgcgggtg tcggcgtcga ggtggtggtg cgcggcatct gcgcgctgcg ccccggggtg cccggcctct ccgagaacat cagggtgcgt tccattctgg gtcggttcct ggagcactcg cggatcctgc acttccgggc gcagaacgag ttctggatcg gcagcgcgga catgatgcac cgcaacctcg accggcgtgt cgaggtgatg gcccaggtca aagacccccg gctgaccgca caactcaacg acatcctcga ctccgcactc gacccggcca cccgatgctg ggaactggga ccggacggtc gatggacacc ctcaccgcgc gagggcgaga cggtgcgcga tcatcaggtg tcgatgatga aacgccgccg ctaccactga
Details for MythA.19275.a.B2.GE40364
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 77kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDR YLNRELSWLD FNARVLALAA DTSLPLLERA KFLAIFASNL DEFYMVRVAG LKRRHETGLS VRSADGLSPL EQLRRISERT QQLATRHADV FLNSVRPALA EEGIVIVTWA ELGEAERNKL STYFHEQVFP VLTPLAVDPA HPFPFVSGLS LNLAIMVKRP EDGGQHFARV KVPDNVDRFV ELDSRPGADG LIRFLPMEEL IAAFLPVLFP GLEIVEHHAF RITRNADFEV EEDRDEDLLQ ALERELARRR FGSPVRLEVS DDMTENMLEL LLRELDVHPD DVVEVPGLLD LSSLWQIYDV DRPALKDPPF VPATPPAFGE RETPKSIFAT LRDGDVLVHH PYDSFSTTVQ RFIEQAAADP NVLAIKQTLY RTSGDSPIVN ALIDAAEAGK QVVALVEIKA RFDEQANIKW ARTLEQAGVH VVYGIIGLKT HCKCALVVRR EGSVIRRYCH IGTGNYNPKT ARLYEDIGLL TAAPDIGADL TDLFNSLTGY SRKESYRNLL VAPYGVRKGL IDRIEGEIAA KREGADAHIR LKANALVDEQ IIDALYRASL AGVGVEVVVR GICALRPGVP GLSENIRVRS ILGRFLEHSR ILHFRAQNEF WIGSADMMHR NLDRRVEVMA QVKDPRLTAQ LNDILDSALD PATRCWELGP DGRWTPSPRE GETVRDHQVS MMKRRRYH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccgct acctcaaccg ggaactcagc tggctggatt tcaacgcccg ggtgctggcg ctggcggccg acacctcgct gcccctgctg gagcgggcga agttcctggc gatcttcgcc tccaacctcg acgagttcta catggtgcgg gtggccgggc tgaaacgccg ccacgagacc ggcctgtcgg tccggtcggc cgacggtctg tcgcccctcg agcagttgcg tcgcatcagt gagcgcaccc agcagctcgc gacccgccac gccgacgtgt tcctcaactc ggtgcggccg gcgctggccg aagagggcat cgtgatcgtc acgtgggccg aactcggtga ggccgaacgc aacaagttgt ccacctactt ccacgagcag gtcttcccgg tcctgacccc gctggcggtc gatccggccc acccgttccc gttcgtcagc ggcctgagcc tcaacctcgc gatcatggtt aaacgccccg aggacggcgg ccagcacttc gcgcgggtca aggtgcccga caacgtcgat cggttcgtcg aactcgacag ccggcccggc gccgacggtc tgatccgttt cctgccgatg gaggaactga tcgcggcgtt cctgccggtg ctgttccccg gcctggagat cgtcgaacac cacgccttcc ggatcacccg caacgcggac ttcgaggtcg aggaggaccg cgacgaggac ctgttgcagg cactggaacg cgaactggcc cggcgccgct tcggttctcc ggtgcgcctc gaggtctccg acgacatgac cgagaacatg ctcgaactgc tcctgcggga actcgacgtc catcccgacg atgtggtcga ggtaccgggg ctgctcgacc tgtcgtcgct gtggcagatc tacgacgtgg accggccggc gctgaaggat ccgccgttcg tgcccgccac cccaccggct ttcggggaac gcgagacacc gaagagcatc ttcgccacgc tgcgcgacgg tgacgtgctg gtgcaccacc cctacgactc gttctccacc accgtccagc gattcatcga gcaggccgcc gccgacccga acgtgttggc gatcaagcag accctgtacc gcacctcggg cgactcgccg atcgtcaacg ccctgatcga cgccgccgag gccggcaagc aggtggtggc gctggtggag atcaaggccc gcttcgacga gcaggccaac atcaagtggg cccgcaccct cgagcaggcc ggcgtccatg tggtgtacgg gatcatcggc ttgaagacgc actgcaagtg cgccctggtg gttcggcggg aaggatcggt gatccggcgc tactgccaca tcggcaccgg caactacaac ccgaaaaccg cacgcctcta cgaagacatc ggactgctca ccgccgcccc ggacatcggc gccgatctga ccgatctgtt caactcgctc accgggtact cccgcaagga gtcctaccgc aacctgctgg tggcgccgta cggcgtccgc aagggactga tcgaccgcat cgagggtgag atcgccgcca aacgcgaggg cgccgacgcc cacatccggt tgaaggccaa tgccctggtc gacgaacaga tcatcgacgc gctgtaccgc gcctcgctcg cgggtgtcgg cgtcgaggtg gtggtgcgcg gcatctgcgc gctgcgcccc ggggtgcccg gcctctccga gaacatcagg gtgcgttcca ttctgggtcg gttcctggag cactcgcgga tcctgcactt ccgggcgcag aacgagttct ggatcggcag cgcggacatg atgcaccgca acctcgaccg gcgtgtcgag gtgatggccc aggtcaaaga cccccggctg accgcacaac tcaacgacat cctcgactcc gcactcgacc cggccacccg atgctgggaa ctgggaccgg acggtcgatg gacaccctca ccgcgcgagg gcgagacggt gcgcgatcat caggtgtcga tgatgaaacg ccgccgctac cactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat