MythA.19107.a

Glycine--tRNA ligase (EC 6.1.1.14) (Glycyl-tRNA synthetase) (GlyRS)

CENTER ID: MythA.19107.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.19107.a.A1.PW39162 full length 1 461
Structures
8SLD
DEPOSITED: 4/21/2023
DETERMINATION: XRay
CLONE: MythA.19107.a.A1.GE44826
PROTEIN: MythA.19107.a.A1.PW39162
8SLF
DEPOSITED: 4/21/2023
DETERMINATION: XRay
CLONE: MythA.19107.a.A1.GE44826
PROTEIN: MythA.19107.a.A1.PW39162
8SLG
DEPOSITED: 4/21/2023
DETERMINATION: XRay
CLONE: MythA.19107.a.A1.GE44826
PROTEIN: MythA.19107.a.A1.PW39162
8SLH
DEPOSITED: 4/21/2023
DETERMINATION: XRay
CLONE: MythA.19107.a.A1.GE44826
PROTEIN: MythA.19107.a.A1.PW39162
8U2Q
DEPOSITED: 9/6/2023
DETERMINATION: XRay
CLONE: MythA.19107.a.UX11.GE44967
PROTEIN: MythA.19107.a.UX11.PW39208
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1078020.3.peg.3308
UniProt: G7CIG9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASIIDTVAN LAKRRGFVYQ SGEIYGGTRS AWDYGPLGVE LKENIKRQWW KSMVTAREDV VGIDTSIILP REVWVASGHV DVFHDPLVEC LNCHRRHRQD HLQEALAGKK GLDNPDDVPM DEVVCPDCGT KGRWTEPREF NMMLKTYLGP IESDEGLHYL RPETAQGIFT NFANVVTTAR KKPPFGIAQT GKSFRNEITP GNFIFRTREF EQMEMEFFVE PSTAKEWHQY WIDTRLQWYV DLGIDRDNLR LYEHPPEKLS HYAERTVDIE YKYGFAGDPW GELEGIANRT DFDLSTHSKH SGVDLSYYDQ ATDTRYVPYV IEPAAGLTRS LMAFLIDAYS EDEAPNAKGG VDKRTVLRFD PRLAPVKVAV LPLSRHADLS PKARDLAAEL RQHWNVEFDD AGAIGRRYRR QDEVGTPYCV TVDFDSLEDN AVTVRERDSM AQERISIDQV TDYLAVRLKG C
NT Sequence
gtggcgtcca tcatcgacac ggttgcaaac ctcgcgaaac ggcgcggctt cgtctaccag tccggcgaga tctacggcgg tacccgctcc gcgtgggact acggtccgct cggggtggaa ctgaaggaga acatcaagcg gcagtggtgg aagtccatgg tcaccgcccg cgaggacgtc gtcggaatcg acacgtcgat catcctgccc cgcgaggtgt gggtggcgtc cggccacgtc gacgtcttcc acgacccgct ggtggagtgc ctcaactgcc accggcggca ccgtcaggac cacctccagg aggcgctggc cggtaagaag gggctggaca acccggacga cgtgccgatg gacgaggtgg tctgcccgga ctgcggcacc aagggccgct ggaccgagcc ccgcgagttc aacatgatgc tcaagaccta cctcgggccg atcgagagcg acgagggtct gcactatctg cggccggaga ccgcgcaggg catcttcacc aacttcgcca acgtggtcac cacggcgcgc aagaagccgc cgttcggcat cgcccagacc ggcaagagct tccgcaacga gatcaccccg ggcaacttca tcttccgcac ccgcgagttc gagcagatgg agatggagtt cttcgtcgag ccgtccaccg ccaaggaatg gcaccagtac tggatcgaca cccggctgca gtggtacgtc gacctcggaa tcgaccggga caacctgcgg ctctacgaac atccgccgga gaagctgtcg cactacgccg aacgcaccgt cgacatcgag tacaagtacg ggttcgccgg cgacccctgg ggtgagctgg agggcatcgc caaccgcacc gatttcgacc tgagcaccca cagcaagcat tccggcgtcg acctgtcgta ctacgaccag gccaccgaca cccgctacgt gccgtacgtc atcgaaccgg cagccgggct cacccggtcg ctgatggcgt tcctgatcga cgcgtactcc gaggacgagg cgcccaacgc caagggcggc gtcgacaagc gcacggtgtt gcggttcgat ccgcggctgg cgccggtcaa ggtggcggtg ctgccgctgt cccggcacgc cgacctgtcg ccgaaggccc gggacctggc cgccgaactg cggcagcact ggaacgtcga gttcgacgac gccggcgcga tcggccggcg ctaccgccgc caggacgagg tcggcacccc gtactgcgtg accgtcgact tcgactccct ggaggacaac gccgtgacgg tccgggagcg cgacagcatg gcccaggagc ggatctcgat cgaccaggtc accgactacc tggccgtccg gctcaagggc tgctga
Details for MythA.19107.a.A1.PW39162
PURIFICATION DATe: 11/16/2022
CONCENTRATION: 16.95mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 14
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASIIDTVA NLAKRRGFVY QSGEIYGGTR SAWDYGPLGV ELKENIKRQW WKSMVTARED VVGIDTSIIL PREVWVASGH VDVFHDPLVE CLNCHRRHRQ DHLQEALAGK KGLDNPDDVP MDEVVCPDCG TKGRWTEPRE FNMMLKTYLG PIESDEGLHY LRPETAQGIF TNFANVVTTA RKKPPFGIAQ TGKSFRNEIT PGNFIFRTRE FEQMEMEFFV EPSTAKEWHQ YWIDTRLQWY VDLGIDRDNL RLYEHPPEKL SHYAERTVDI EYKYGFAGDP WGELEGIANR TDFDLSTHSK HSGVDLSYYD QATDTRYVPY VIEPAAGLTR SLMAFLIDAY SEDEAPNAKG GVDKRTVLRF DPRLAPVKVA VLPLSRHADL SPKARDLAAE LRQHWNVEFD DAGAIGRRYR RQDEVGTPYC VTVDFDSLED NAVTVRERDS MAQERISIDQ VTDYLAVRLK GC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GGCGTCCATC ATCGACACGG TTGCAAACCT CGCGAAACGG CGCGGCTTCG TCTACCAGTC CGGCGAGATC TACGGCGGTA CCCGCTCCGC GTGGGACTAC GGTCCGCTCG GGGTGGAACT GAAGGAGAAC ATCAAGCGGC AGTGGTGGAA GTCCATGGTC ACCGCCCGCG AGGACGTCGT CGGAATCGAC ACGTCGATCA TCCTGCCCCG CGAGGTGTGG GTGGCGTCCG GCCACGTCGA CGTCTTCCAC GACCCGCTGG TGGAGTGCCT CAACTGCCAC CGGCGGCACC GTCAGGACCA CCTCCAGGAG GCGCTGGCCG GTAAGAAGGG GCTGGACAAC CCGGACGACG TGCCGATGGA CGAGGTGGTC TGCCCGGACT GCGGCACCAA GGGCCGCTGG ACCGAGCCCC GCGAGTTCAA CATGATGCTC AAGACCTACC TCGGGCCGAT CGAGAGCGAC GAGGGTCTGC ACTATCTGCG GCCGGAGACC GCGCAGGGCA TCTTCACCAA CTTCGCCAAC GTGGTCACCA CGGCGCGCAA GAAGCCGCCG TTCGGCATCG CCCAGACCGG CAAGAGCTTC CGCAACGAGA TCACCCCGGG CAACTTCATC TTCCGCACCC GCGAGTTCGA GCAGATGGAG ATGGAGTTCT TCGTCGAGCC GTCCACCGCC AAGGAATGGC ACCAGTACTG GATCGACACC CGGCTGCAGT GGTACGTCGA CCTCGGAATC GACCGGGACA ACCTGCGGCT CTACGAACAT CCGCCGGAGA AGCTGTCGCA CTACGCCGAA CGCACCGTCG ACATCGAGTA CAAGTACGGG TTCGCCGGCG ACCCCTGGGG TGAGCTGGAG GGCATCGCCA ACCGCACCGA TTTCGACCTG AGCACCCACA GCAAGCATTC CGGCGTCGAC CTGTCGTACT ACGACCAGGC CACCGACACC CGCTACGTGC CGTACGTCAT CGAACCGGCA GCCGGGCTCA CCCGGTCGCT GATGGCGTTC CTGATCGACG CGTACTCCGA GGACGAGGCG CCCAACGCCA AGGGCGGCGT CGACAAGCGC ACGGTGTTGC GGTTCGATCC GCGGCTGGCG CCGGTCAAGG TGGCGGTGCT GCCGCTGTCC CGGCACGCCG ACCTGTCGCC GAAGGCCCGG GACCTGGCCG CCGAACTGCG GCAGCACTGG AACGTCGAGT TCGACGACGC CGGCGCGATC GGCCGGCGCT ACCGCCGCCA GGACGAGGTC GGCACCCCGT ACTGCGTGAC CGTCGACTTC GACTCCCTGG AGGACAACGC CGTGACGGTC CGGGAGCGCG ACAGCATGGC CCAGGAGCGG ATCTCGATCG ACCAGGTCAC CGACTACCTG GCCGTCCGGC TCAAGGGCTG CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA