MythA.18889.a

Alanine--tRNA ligase (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS)

CENTER ID: MythA.18889.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.18889.a.A1.PW39161 full length 1 902

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1945
UniProt: G7CG52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTHEIRKRF LDHFVKAGHT EVPSASVILD DPNLLFVNAG MVQFVPYFLG QRTPPYTRAT SIQKCIRTPD IDEVGITTRH NTFFQMAGNF SFGDYFKRGA IEYAWSLLTK SVDDGGYGFD PEKIWATVYL DDDEAADLWQ EIAGLPPERI QRRGMADNYW SMGIPGPCGP SSEIYYDRGP DYGVEGGPIA NEDRYIEIWN LVFMENERGA GPAKEGYEIL GPLPRKNIDT GMGIERVACL LQGVDNVYET DLIRPVIDLV AGLAPRGYGQ GSHSDDVRYR IIADHSRTAA ILIGDGVTPG NDGRGYVLRR LLRRVIRSAK LLDVDRPIVG DLMATVRDTM GPAYGNLISD FDRIHRIAVA EETAFNRTLS SGSKLFEEAA KATKAAGATV LSGKDAFTLH DTYGFPLELT LEMAAEAGLS VDEEGFRTLM AEQRERAKAD AAARKQAHAD LSAYRELVDA GPTEFTGFDE LTSEAKILGI FVDGQRVPVV SHLPRVRSQS PPPQQVEIVL DRTPFYAESG GQIADEGTIT GTGSSMAARA RVTDVQKIAN TLFVHRVSME SGEFVEGDTV IAAVDPEWRR GATQGHSGTH MVHAALRQVL GPNAVQAGSL NRPGYLRFDF NWQGPLSEQQ RTQIEEVTNE AVQADFEVNT FITELEKAKS MGAIAMFGEK YPERVRVVEI GGPFSLELCG GTHVESSAQI GPVTILGESS VGSGVRRVEA YVGLDSFRHL SKERALMAAV ASTLKVPSEE VPDRVANLVE KLRAAEKELE RLRLANAKAA VSDAVAQAEQ VGRVRLVARR IDAGLNAGEL RNLVGDIRGK LGSDPAVVAL MAATDDGSVP FVVAVNPAAQ DLGLRANNLV KVLAPEVDGR GGGKVDLAQG SGKKAAGIDA ALAALRAEIA RS
NT Sequence
gtgcagacac acgagatcag gaagcggttc ctcgatcatt ttgtgaaggc gggccacacg gaggtgccga gcgcctcggt gatcctcgac gaccccaatc tgttgttcgt caacgccggc atggtgcagt tcgtgcccta tttcctcggg cagcgcaccc cgccgtacac ccgggcgacg agcatccaga agtgcatccg caccccggac atcgacgagg tcggtatcac cacccggcac aacacgttct tccagatggc cgggaacttc tcgttcggcg actacttcaa acgcggcgcg atcgagtacg cctggagcct gctgaccaag tcggtggacg acggcggcta cggcttcgat cccgaaaaga tctgggccac cgtttatctc gatgacgacg aggccgcgga tctgtggcag gagatcgccg gtctgccgcc ggagcggatc cagcgccgcg gcatggccga caactactgg tcgatgggca tccccgggcc gtgcggaccg tcgtcggaga tctactacga ccgcgggccg gactacggcg tcgagggcgg cccgatcgcc aacgaggacc gctacatcga gatctggaat ctcgtgttca tggagaacga gcgcggcgcc gggccggcca aggagggcta cgagatcctc ggcccgctgc cgcggaagaa catcgacacc ggcatgggca tcgagcgggt ggcctgcctg ctgcagggcg tcgacaacgt ctacgagacg gatctgatcc gcccggtgat cgatctggtc gccggcctgg cgccacgggg ctacggccag ggcagtcatt ccgacgatgt gcggtaccgg atcatcgccg accacagccg taccgcggcg atcctgatcg gggacggggt gaccccgggt aacgacggcc gcggctatgt gctgcgccgg ttgctgcggc gggtcatccg gtcggccaag ctgctcgacg tggaccgccc gatcgtcggc gatctgatgg ccacggtgcg ggacaccatg ggccccgcct acggcaacct gatcagcgac ttcgaccgga tccaccgcat cgcggtggcc gaggagaccg cgttcaaccg cactctgtcg tcggggtcga agctgttcga ggaggccgcc aaggccacca aggccgccgg cgccaccgtg ctgagcggca aggacgcgtt cacgctgcac gacacctacg gcttcccgct cgagctgacc ctggagatgg ccgccgaggc cgggctgtcg gtggatgagg agggtttccg gaccctgatg gccgagcagc gcgaacgcgc caaggccgac gccgccgccc gcaaacaggc ccatgccgac ctgtcggcgt atcgcgaact ggtcgacgcc ggacccaccg agttcaccgg gttcgacgaa ttgacttccg aggcaaagat tctcggtatc ttcgtggacg gtcagcgggt gccggtggtg tcgcatctgc cgcgggtgcg ctcgcagagc ccgcctccgc aacaggtcga gatcgtgctg gaccgcaccc cgttctacgc cgagtccggt gggcagatcg ccgacgaggg caccatcacg ggcaccggtt cgtcgatggc cgcgcgcgcc cgggtcaccg atgtgcagaa gatcgccaac acactgttcg tgcaccgggt cagcatggag tccggcgagt tcgtcgaggg ggacaccgtg atcgccgctg tggaccccga gtggcggcgc ggggcgaccc agggccactc cggcacccac atggtgcatg cggcgctgcg gcaggtgctg ggccccaacg cggttcaggc cggttcgctg aaccggccgg gttatctgcg gttcgacttc aactggcagg gcccgttgtc cgagcagcag cgcacccaga tcgaggaggt gaccaacgag gcggtccagg ccgacttcga ggtgaacacc ttcatcaccg aactcgagaa ggccaagtcg atgggcgcca tcgcgatgtt cggcgagaag taccccgagc gggtccgggt ggtggagatc ggcggtccgt tctcgttgga actgtgcggc ggcacccatg tggagagctc cgcgcagatc ggcccggtga cgatcctggg cgagtcctcg gtgggttcgg gggtgcgccg ggtcgaggcg tacgtcgggc tcgactcctt ccgccacctg agcaaggaac gcgcgctgat ggccgcggtg gcctcgacgc tgaaggtgcc ctccgaggag gtgcccgacc gggtggccaa tctggtggag aagctacggg ccgccgagaa ggaactggaa cggctgcggc tggccaacgc caaggcggcg gtcagcgatg cggtggccca ggccgagcag gtcggcaggg tgcggctggt ggcccggcgc atcgacgccg gcctgaacgc cggtgagctg cgcaacctgg tcggcgacat ccgcggcaag ctgggctcgg atccggcggt ggtggcgctg atggcggcca ccgacgacgg ctcggtgccg ttcgtggtgg cggtcaaccc ggccgcccag gacctcgggc tgcgggccaa caacctggtc aaggtgctgg ccccggaggt cgacggtcgc ggcggcggca aggtggacct ggctcagggg tccggtaaga aggcggcggg catcgatgcg gcactggccg cgctacgcgc ggagattgcc cggagctag
Details for MythA.18889.a.A1.PW39161
PURIFICATION DATe: 11/2/2022
CONCENTRATION: 13.84mg/ml
OBSERVED MW: 100kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQTHEIRKR FLDHFVKAGH TEVPSASVIL DDPNLLFVNA GMVQFVPYFL GQRTPPYTRA TSIQKCIRTP DIDEVGITTR HNTFFQMAGN FSFGDYFKRG AIEYAWSLLT KSVDDGGYGF DPEKIWATVY LDDDEAADLW QEIAGLPPER IQRRGMADNY WSMGIPGPCG PSSEIYYDRG PDYGVEGGPI ANEDRYIEIW NLVFMENERG AGPAKEGYEI LGPLPRKNID TGMGIERVAC LLQGVDNVYE TDLIRPVIDL VAGLAPRGYG QGSHSDDVRY RIIADHSRTA AILIGDGVTP GNDGRGYVLR RLLRRVIRSA KLLDVDRPIV GDLMATVRDT MGPAYGNLIS DFDRIHRIAV AEETAFNRTL SSGSKLFEEA AKATKAAGAT VLSGKDAFTL HDTYGFPLEL TLEMAAEAGL SVDEEGFRTL MAEQRERAKA DAAARKQAHA DLSAYRELVD AGPTEFTGFD ELTSEAKILG IFVDGQRVPV VSHLPRVRSQ SPPPQQVEIV LDRTPFYAES GGQIADEGTI TGTGSSMAAR ARVTDVQKIA NTLFVHRVSM ESGEFVEGDT VIAAVDPEWR RGATQGHSGT HMVHAALRQV LGPNAVQAGS LNRPGYLRFD FNWQGPLSEQ QRTQIEEVTN EAVQADFEVN TFITELEKAK SMGAIAMFGE KYPERVRVVE IGGPFSLELC GGTHVESSAQ IGPVTILGES SVGSGVRRVE AYVGLDSFRH LSKERALMAA VASTLKVPSE EVPDRVANLV EKLRAAEKEL ERLRLANAKA AVSDAVAQAE QVGRVRLVAR RIDAGLNAGE LRNLVGDIRG KLGSDPAVVA LMAATDDGSV PFVVAVNPAA QDLGLRANNL VKVLAPEVDG RGGGKVDLAQ GSGKKAAGID AALAALRAEI ARS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GCAGACACAC GAGATCAGGA AGCGGTTCCT CGATCATTTT GTGAAGGCGG GCCACACGGA GGTGCCGAGC GCCTCGGTGA TCCTCGACGA CCCCAATCTG TTGTTCGTCA ACGCCGGCAT GGTGCAGTTC GTGCCCTATT TCCTCGGGCA GCGCACCCCG CCGTACACCC GGGCGACGAG CATCCAGAAG TGCATCCGCA CCCCGGACAT CGACGAGGTC GGTATCACCA CCCGGCACAA CACGTTCTTC CAGATGGCCG GGAACTTCTC GTTCGGCGAC TACTTCAAAC GCGGCGCGAT CGAGTACGCC TGGAGCCTGC TGACCAAGTC GGTGGACGAC GGCGGCTACG GCTTCGATCC CGAAAAGATC TGGGCCACCG TTTATCTCGA TGACGACGAG GCCGCGGATC TGTGGCAGGA GATCGCCGGT CTGCCGCCGG AGCGGATCCA GCGCCGCGGC ATGGCCGACA ACTACTGGTC GATGGGCATC CCCGGGCCGT GCGGACCGTC GTCGGAGATC TACTACGACC GCGGGCCGGA CTACGGCGTC GAGGGCGGCC CGATCGCCAA CGAGGACCGC TACATCGAGA TCTGGAATCT CGTGTTCATG GAGAACGAGC GCGGCGCCGG GCCGGCCAAG GAGGGCTACG AGATCCTCGG CCCGCTGCCG CGGAAGAACA TCGACACCGG CATGGGCATC GAGCGGGTGG CCTGCCTGCT GCAGGGCGTC GACAACGTCT ACGAGACGGA TCTGATCCGC CCGGTGATCG ATCTGGTCGC CGGCCTGGCG CCACGGGGCT ACGGCCAGGG CAGTCATTCC GACGATGTGC GGTACCGGAT CATCGCCGAC CACAGCCGTA CCGCGGCGAT CCTGATCGGG GACGGGGTGA CCCCGGGTAA CGACGGCCGC GGCTATGTGC TGCGCCGGTT GCTGCGGCGG GTCATCCGGT CGGCCAAGCT GCTCGACGTG GACCGCCCGA TCGTCGGCGA TCTGATGGCC ACGGTGCGGG ACACCATGGG CCCCGCCTAC GGCAACCTGA TCAGCGACTT CGACCGGATC CACCGCATCG CGGTGGCCGA GGAGACCGCG TTCAACCGCA CTCTGTCGTC GGGGTCGAAG CTGTTCGAGG AGGCCGCCAA GGCCACCAAG GCCGCCGGCG CCACCGTGCT GAGCGGCAAG GACGCGTTCA CGCTGCACGA CACCTACGGC TTCCCGCTCG AGCTGACCCT GGAGATGGCC GCCGAGGCCG GGCTGTCGGT GGATGAGGAG GGTTTCCGGA CCCTGATGGC CGAGCAGCGC GAACGCGCCA AGGCCGACGC CGCCGCCCGC AAACAGGCCC ATGCCGACCT GTCGGCGTAT CGCGAACTGG TCGACGCCGG ACCCACCGAG TTCACCGGGT TCGACGAATT GACTTCCGAG GCAAAGATTC TCGGTATCTT CGTGGACGGT CAGCGGGTGC CGGTGGTGTC GCATCTGCCG CGGGTGCGCT CGCAGAGCCC GCCTCCGCAA CAGGTCGAGA TCGTGCTGGA CCGCACCCCG TTCTACGCCG AGTCCGGTGG GCAGATCGCC GACGAGGGCA CCATCACGGG CACCGGTTCG TCGATGGCCG CGCGCGCCCG GGTCACCGAT GTGCAGAAGA TCGCCAACAC ACTGTTCGTG CACCGGGTCA GCATGGAGTC CGGCGAGTTC GTCGAGGGGG ACACCGTGAT CGCCGCTGTG GACCCCGAGT GGCGGCGCGG GGCGACCCAG GGCCACTCCG GCACCCACAT GGTGCATGCG GCGCTGCGGC AGGTGCTGGG CCCCAACGCG GTTCAGGCCG GTTCGCTGAA CCGGCCGGGT TATCTGCGGT TCGACTTCAA CTGGCAGGGC CCGTTGTCCG AGCAGCAGCG CACCCAGATC GAGGAGGTGA CCAACGAGGC GGTCCAGGCC GACTTCGAGG TGAACACCTT CATCACCGAA CTCGAGAAGG CCAAGTCGAT GGGCGCCATC GCGATGTTCG GCGAGAAGTA CCCCGAGCGG GTCCGGGTGG TGGAGATCGG CGGTCCGTTC TCGTTGGAAC TGTGCGGCGG CACCCATGTG GAGAGCTCCG CGCAGATCGG CCCGGTGACG ATCCTGGGCG AGTCCTCGGT GGGTTCGGGG GTGCGCCGGG TCGAGGCGTA CGTCGGGCTC GACTCCTTCC GCCACCTGAG CAAGGAACGC GCGCTGATGG CCGCGGTGGC CTCGACGCTG AAGGTGCCCT CCGAGGAGGT GCCCGACCGG GTGGCCAATC TGGTGGAGAA GCTACGGGCC GCCGAGAAGG AACTGGAACG GCTGCGGCTG GCCAACGCCA AGGCGGCGGT CAGCGATGCG GTGGCCCAGG CCGAGCAGGT CGGCAGGGTG CGGCTGGTGG CCCGGCGCAT CGACGCCGGC CTGAACGCCG GTGAGCTGCG CAACCTGGTC GGCGACATCC GCGGCAAGCT GGGCTCGGAT CCGGCGGTGG TGGCGCTGAT GGCGGCCACC GACGACGGCT CGGTGCCGTT CGTGGTGGCG GTCAACCCGG CCGCCCAGGA CCTCGGGCTG CGGGCCAACA ACCTGGTCAA GGTGCTGGCC CCGGAGGTCG ACGGTCGCGG CGGCGGCAAG GTGGACCTGG CTCAGGGGTC CGGTAAGAAG GCGGCGGGCA TCGATGCGGC ACTGGCCGCG CTACGCGCGG AGATTGCCCG GAGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA