SSGCID: Target Page

MythA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MythA.17943.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.17943.a.B11.GE42613	full length		1	950

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.17943.a.B11.PW38528	full length		1	950

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2089
UniProt:	G7CIL7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDSTSTDTT DTPPFRYTAK LAGEIERRWQ QTWAEQGTFH VPNPVGSLAP PDGSPVPADK MFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYHRMRGRNV LHALGFDAFG LPAEQYAVQT GTHPRTRTEA NIANFRRQLG RLGLGHDDRR SFSTTDVEYY KWTQWIFLQI FNAWFDPDQN KARPISELVA EFESGARPVG DGRSWSELPA GERHDIIDSY RLVYRADSMV NWCPGLGTVL ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYAERLLED LDVLDWPEKV KTMQRNWIGR STGAAVLFAT DAGDIEVFTT RPDTLFGATY MVLAPEHDMV DQLVADRWPD GVDPRWTLGA GTPKEAVSTY RAAIAAKSDL ERQENKDKTG VFLGAYATNP ANGERIPVFI ADYVLLGYGT GAIMAVPGHD QRDWEFAREF GLPIVEVIAG GNISEAAHTG DGELVNSGFL NGMNVEDAKD AMTSRLAAEG RGEARVEYKL RDWLFARQRY WGEPFPIVYD SDGRPHPLPE SMLPVELPDI PDYAPVSFDP DDADSEPSPP LNKATDWVTV ELDLGNGLKT YTRDTNVMPQ WAGSSWYELR YCDPHNSEEM CAKENEAYWM GPRPEIHGPD DPGGVDLYVG GVEHAVLHLL YARFWHKVLY DLGHVTSREP YRRLVNQGYI QAYAYTDPRG SYVPAAEVIE RDGRFYWPGP DGEIEVKQEF GKIGKSLKNS ISPDEICDEY GADTLRVYEM SMGPLEASRP WAPKDVVGAY RFLQRVWRLV VDEETGATRV AAHEAVDEQT QRLLHKTIAG VSEDYAALRN NTAAAKLIEY TNHLTKENVT ARAAIEPLLL MLAPLAPHLA EELWHRVGNT GSLAHGPFPE ADPQYLVEDT VEMPVQVNGK VRGHITVAVD ADKATLEAAA LADEKVQAFL AGATPKKVIV VPGKLVNLVV

NT Sequence

gtgaccgact cgaccagcac cgacaccacc gacacgccgc cgttccgcta caccgcgaag ctggccggcg agatcgagcg gcgctggcag cagacctggg cggagcaggg cacgttccat gtgcccaacc cggtgggctc gttggcgccg ccggacggca gcccggtgcc cgccgacaag atgttcgtgc aggacatgtt cccgtacccg tccggggagg ggctgcacgt cggacatccg ctcggctaca tcgccaccga cgtgtacgcg cggtaccacc ggatgcgggg ccgcaatgtg ttgcatgcgt tgggtttcga cgcgttcggc ctgccagccg aacagtacgc ggtgcagacc ggcacccatc cgcgcacccg caccgaggcc aacatcgcca acttccggcg gcagctgggc cggctcggcc tgggccatga cgaccggcgc agtttctcca ccaccgacgt ggagtactac aagtggaccc agtggatctt cctgcagatc ttcaacgcgt ggttcgaccc ggaccagaac aaggcccgcc cgatcagcga gctggtggcg gagttcgagt ccggtgcccg gccggtcggc gacggacggt cctggtcgga gctgccggcc ggggagcggc acgacatcat cgactcgtac cggttggtgt accgcgccga ctcgatggtc aactggtgcc ccggcctggg caccgtgctg gccaacgaag aggtcaccgc cgacggccgc agcgaccgcg gcaacttccc agtgttccgg aagcggttgc ggcagtggat gatgcgcatc accgcgtatg ccgaacggct gctcgaggac ctcgacgtgc tggactggcc ggagaaggtc aagaccatgc agcgcaactg gatcgggcgg tcgaccggcg ccgcggtgct gttcgccacc gatgccggcg acatcgaggt gttcaccacc cggcccgaca ccctgttcgg cgccacctac atggtgctgg cccccgaaca cgacatggtg gaccaactgg tcgccgaccg gtggccggac ggcgtggacc cgcgctggac cctcggcgcc ggcacgccga aggaggcggt cagcacctac cgggccgcga tcgccgcgaa gtcggacctg gaacggcagg agaacaagga caagaccggc gtcttcctcg gcgcgtatgc caccaatccg gccaacggcg aacgcattcc ggtgttcatc gccgactatg tgctgctcgg ctacggcacc ggggcgatca tggcggtgcc cggccacgat cagcgggact gggagttcgc ccgggaattc ggattgccga tcgtggaagt gattgccggc ggaaatatct cggaggcggc ccacaccggt gacggtgaac tggtgaactc cgggttcctc aacgggatga atgtcgagga cgccaaggat gcgatgacgt cgcgactggc ggcggagggc cgcggtgagg cccgggtcga atacaagctg cgggactggc ttttcgcccg gcaacggtac tggggcgagc cgttcccgat cgtctacgac tcggacggcc gcccgcatcc gctaccggaa tcgatgctgc cggtggaact tccggacatc cccgactacg cgccggtgtc gttcgacccg gacgacgcgg acagcgaacc gtcgccgccg ctgaacaagg cgaccgactg ggtgaccgtc gaactcgatc tcggtaacgg gctcaagacc tacacccggg acaccaacgt gatgccgcag tgggcgggca gctcgtggta cgagttgcgc tactgcgatc cgcacaactc agaagagatg tgcgccaagg agaatgaggc gtactggatg ggtccgcggc cggagattca cggcccggac gatccgggtg gtgtcgatct gtacgtcggc ggggtggagc atgcggtgct gcacctgctg tatgcccggt tctggcacaa ggtgctctac gacctgggcc acgtcacctc ccgggagccg taccggcggc tggtcaacca gggctacatc caggcctacg cctacaccga tccgcgcggc agctatgtgc cggccgcgga ggtgatcgag cgggacggca ggttctactg gcccgggccg gacggcgaga tcgaggtcaa acaggagttc ggcaagatcg gcaagagcct gaagaactcc atctcgcccg acgagatctg tgacgagtac ggcgcggaca cactgcgggt gtacgagatg tcgatgggcc cgctggaggc gtcacggccg tgggcgccca aggacgtcgt gggggcctac cggttcctgc agcgggtgtg gcggctggtc gtcgacgagg agaccggcgc cacccgggtg gccgcgcacg aggcggtgga cgaacagaca cagcggttgc tgcacaagac gatcgcgggg gtgtcggagg actacgcggc gctgcgcaac aacaccgcgg cggccaagct gatcgagtac accaaccatc tgaccaagga gaatgtcacc gcccgggcgg ccatcgagcc gttgctgctg atgctcgccc cgctggcgcc gcatctagcc gaggagctgt ggcaccgggt gggcaacacc ggatcgctgg cgcacgggcc gttcccggag gccgacccgc agtatctggt cgaggacacc gtcgagatgc cggtgcaggt caacggcaag gtgcgcggac acatcacggt cgcggtggac gccgacaagg cgacgctgga ggccgcggcg ctggccgatg agaaggtgca ggctttcctg gccggcgcca ctccgaagaa ggtgatcgtg gtccccggca agctcgtcaa cctcgtcgtc tga

Details for MythA.17943.a.B11.GE42613

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT DSTSTDTTDT PPFRYTAKLA GEIERRWQQT WAEQGTFHVP NPVGSLAPPD GSPVPADKMF VQDMFPYPSG EGLHVGHPLG YIATDVYARY HRMRGRNVLH ALGFDAFGLP AEQYAVQTGT HPRTRTEANI ANFRRQLGRL GLGHDDRRSF STTDVEYYKW TQWIFLQIFN AWFDPDQNKA RPISELVAEF ESGARPVGDG RSWSELPAGE RHDIIDSYRL VYRADSMVNW CPGLGTVLAN EEVTADGRSD RGNFPVFRKR LRQWMMRITA YAERLLEDLD VLDWPEKVKT MQRNWIGRST GAAVLFATDA GDIEVFTTRP DTLFGATYMV LAPEHDMVDQ LVADRWPDGV DPRWTLGAGT PKEAVSTYRA AIAAKSDLER QENKDKTGVF LGAYATNPAN GERIPVFIAD YVLLGYGTGA IMAVPGHDQR DWEFAREFGL PIVEVIAGGN ISEAAHTGDG ELVNSGFLNG MNVEDAKDAM TSRLAAEGRG EARVEYKLRD WLFARQRYWG EPFPIVYDSD GRPHPLPESM LPVELPDIPD YAPVSFDPDD ADSEPSPPLN KATDWVTVEL DLGNGLKTYT RDTNVMPQWA GSSWYELRYC DPHNSEEMCA KENEAYWMGP RPEIHGPDDP GGVDLYVGGV EHAVLHLLYA RFWHKVLYDL GHVTSREPYR RLVNQGYIQA YAYTDPRGSY VPAAEVIERD GRFYWPGPDG EIEVKQEFGK IGKSLKNSIS PDEICDEYGA DTLRVYEMSM GPLEASRPWA PKDVVGAYRF LQRVWRLVVD EETGATRVAA HEAVDEQTQR LLHKTIAGVS EDYAALRNNT AAAKLIEYTN HLTKENVTAR AAIEPLLLML APLAPHLAEE LWHRVGNTGS LAHGPFPEAD PQYLVEDTVE MPVQVNGKVR GHITVAVDAD KATLEAAALA DEKVQAFLAG ATPKKVIVVP GKLVNLVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg actcgaccag caccgacacc accgacacgc cgccgttccg ctacaccgcg aagctggccg gcgagatcga gcggcgctgg cagcagacct gggcggagca gggcacgttc catgtgccca acccggtggg ctcgttggcg ccgccggacg gcagcccggt gcccgccgac aagatgttcg tgcaggacat gttcccgtac ccgtccgggg aggggctgca cgtcggacat ccgctcggct acatcgccac cgacgtgtac gcgcggtacc accggatgcg gggccgcaat gtgttgcatg cgttgggttt cgacgcgttc ggcctgccag ccgaacagta cgcggtgcag accggcaccc atccgcgcac ccgcaccgag gccaacatcg ccaacttccg gcggcagctg ggccggctcg gcctgggcca tgacgaccgg cgcagtttct ccaccaccga cgtggagtac tacaagtgga cccagtggat cttcctgcag atcttcaacg cgtggttcga cccggaccag aacaaggccc gcccgatcag cgagctggtg gcggagttcg agtccggtgc ccggccggtc ggcgacggac ggtcctggtc ggagctgccg gccggggagc ggcacgacat catcgactcg taccggttgg tgtaccgcgc cgactcgatg gtcaactggt gccccggcct gggcaccgtg ctggccaacg aagaggtcac cgccgacggc cgcagcgacc gcggcaactt cccagtgttc cggaagcggt tgcggcagtg gatgatgcgc atcaccgcgt atgccgaacg gctgctcgag gacctcgacg tgctggactg gccggagaag gtcaagacca tgcagcgcaa ctggatcggg cggtcgaccg gcgccgcggt gctgttcgcc accgatgccg gcgacatcga ggtgttcacc acccggcccg acaccctgtt cggcgccacc tacatggtgc tggcccccga acacgacatg gtggaccaac tggtcgccga ccggtggccg gacggcgtgg acccgcgctg gaccctcggc gccggcacgc cgaaggaggc ggtcagcacc taccgggccg cgatcgccgc gaagtcggac ctggaacggc aggagaacaa ggacaagacc ggcgtcttcc tcggcgcgta tgccaccaat ccggccaacg gcgaacgcat tccggtgttc atcgccgact atgtgctgct cggctacggc accggggcga tcatggcggt gcccggccac gatcagcggg actgggagtt cgcccgggaa ttcggattgc cgatcgtgga agtgattgcc ggcggaaata tctcggaggc ggcccacacc ggtgacggtg aactggtgaa ctccgggttc ctcaacggga tgaatgtcga ggacgccaag gatgcgatga cgtcgcgact ggcggcggag ggccgcggtg aggcccgggt cgaatacaag ctgcgggact ggcttttcgc ccggcaacgg tactggggcg agccgttccc gatcgtctac gactcggacg gccgcccgca tccgctaccg gaatcgatgc tgccggtgga acttccggac atccccgact acgcgccggt gtcgttcgac ccggacgacg cggacagcga accgtcgccg ccgctgaaca aggcgaccga ctgggtgacc gtcgaactcg atctcggtaa cgggctcaag acctacaccc gggacaccaa cgtgatgccg cagtgggcgg gcagctcgtg gtacgagttg cgctactgcg atccgcacaa ctcagaagag atgtgcgcca aggagaatga ggcgtactgg atgggtccgc ggccggagat tcacggcccg gacgatccgg gtggtgtcga tctgtacgtc ggcggggtgg agcatgcggt gctgcacctg ctgtatgccc ggttctggca caaggtgctc tacgacctgg gccacgtcac ctcccgggag ccgtaccggc ggctggtcaa ccagggctac atccaggcct acgcctacac cgatccgcgc ggcagctatg tgccggccgc ggaggtgatc gagcgggacg gcaggttcta ctggcccggg ccggacggcg agatcgaggt caaacaggag ttcggcaaga tcggcaagag cctgaagaac tccatctcgc ccgacgagat ctgtgacgag tacggcgcgg acacactgcg ggtgtacgag atgtcgatgg gcccgctgga ggcgtcacgg ccgtgggcgc ccaaggacgt cgtgggggcc taccggttcc tgcagcgggt gtggcggctg gtcgtcgacg aggagaccgg cgccacccgg gtggccgcgc acgaggcggt ggacgaacag acacagcggt tgctgcacaa gacgatcgcg ggggtgtcgg aggactacgc ggcgctgcgc aacaacaccg cggcggccaa gctgatcgag tacaccaacc atctgaccaa ggagaatgtc accgcccggg cggccatcga gccgttgctg ctgatgctcg ccccgctggc gccgcatcta gccgaggagc tgtggcaccg ggtgggcaac accggatcgc tggcgcacgg gccgttcccg gaggccgacc cgcagtatct ggtcgaggac accgtcgaga tgccggtgca ggtcaacggc aaggtgcgcg gacacatcac ggtcgcggtg gacgccgaca aggcgacgct ggaggccgcg gcgctggccg atgagaaggt gcaggctttc ctggccggcg ccactccgaa gaaggtgatc gtggtccccg gcaagctcgt caacctcgtc gtctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for MythA.17943.a.B11.PW38528

PURIFICATION DATe:	9/24/2018
CONCENTRATION:	14.52mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACCG ACTCGACCAG CACCGACACC ACCGACACGC CGCCGTTCCG CTACACCGCG AAGCTGGCCG GCGAGATCGA GCGGCGCTGG CAGCAGACCT GGGCGGAGCA GGGCACGTTC CATGTGCCCA ACCCGGTGGG CTCGTTGGCG CCGCCGGACG GCAGCCCGGT GCCCGCCGAC AAGATGTTCG TGCAGGACAT GTTCCCGTAC CCGTCCGGGG AGGGGCTGCA CGTCGGACAT CCGCTCGGCT ACATCGCCAC CGACGTGTAC GCGCGGTACC ACCGGATGCG GGGCCGCAAT GTGTTGCATG CGTTGGGTTT CGACGCGTTC GGCCTGCCAG CCGAACAGTA CGCGGTGCAG ACCGGCACCC ATCCGCGCAC CCGCACCGAG GCCAACATCG CCAACTTCCG GCGGCAGCTG GGCCGGCTCG GCCTGGGCCA TGACGACCGG CGCAGTTTCT CCACCACCGA CGTGGAGTAC TACAAGTGGA CCCAGTGGAT CTTCCTGCAG ATCTTCAACG CGTGGTTCGA CCCGGACCAG AACAAGGCCC GCCCGATCAG CGAGCTGGTG GCGGAGTTCG AGTCCGGTGC CCGGCCGGTC GGCGACGGAC GGTCCTGGTC GGAGCTGCCG GCCGGGGAGC GGCACGACAT CATCGACTCG TACCGGTTGG TGTACCGCGC CGACTCGATG GTCAACTGGT GCCCCGGCCT GGGCACCGTG CTGGCCAACG AAGAGGTCAC CGCCGACGGC CGCAGCGACC GCGGCAACTT CCCAGTGTTC CGGAAGCGGT TGCGGCAGTG GATGATGCGC ATCACCGCGT ATGCCGAACG GCTGCTCGAG GACCTCGACG TGCTGGACTG GCCGGAGAAG GTCAAGACCA TGCAGCGCAA CTGGATCGGG CGGTCGACCG GCGCCGCGGT GCTGTTCGCC ACCGATGCCG GCGACATCGA GGTGTTCACC ACCCGGCCCG ACACCCTGTT CGGCGCCACC TACATGGTGC TGGCCCCCGA ACACGACATG GTGGACCAAC TGGTCGCCGA CCGGTGGCCG GACGGCGTGG ACCCGCGCTG GACCCTCGGC GCCGGCACGC CGAAGGAGGC GGTCAGCACC TACCGGGCCG CGATCGCCGC GAAGTCGGAC CTGGAACGGC AGGAGAACAA GGACAAGACC GGCGTCTTCC TCGGCGCGTA TGCCACCAAT CCGGCCAACG GCGAACGCAT TCCGGTGTTC ATCGCCGACT ATGTGCTGCT CGGCTACGGC ACCGGGGCGA TCATGGCGGT GCCCGGCCAC GATCAGCGGG ACTGGGAGTT CGCCCGGGAA TTCGGATTGC CGATCGTGGA AGTGATTGCC GGCGGAAATA TCTCGGAGGC GGCCCACACC GGTGACGGTG AACTGGTGAA CTCCGGGTTC CTCAACGGGA TGAATGTCGA GGACGCCAAG GATGCGATGA CGTCGCGACT GGCGGCGGAG GGCCGCGGTG AGGCCCGGGT CGAATACAAG CTGCGGGACT GGCTTTTCGC CCGGCAACGG TACTGGGGCG AGCCGTTCCC GATCGTCTAC GACTCGGACG GCCGCCCGCA TCCGCTACCG GAATCGATGC TGCCGGTGGA ACTTCCGGAC ATCCCCGACT ACGCGCCGGT GTCGTTCGAC CCGGACGACG CGGACAGCGA ACCGTCGCCG CCGCTGAACA AGGCGACCGA CTGGGTGACC GTCGAACTCG ATCTCGGTAA CGGGCTCAAG ACCTACACCC GGGACACCAA CGTGATGCCG CAGTGGGCGG GCAGCTCGTG GTACGAGTTG CGCTACTGCG ATCCGCACAA CTCAGAAGAG ATGTGCGCCA AGGAGAATGA GGCGTACTGG ATGGGTCCGC GGCCGGAGAT TCACGGCCCG GACGATCCGG GTGGTGTCGA TCTGTACGTC GGCGGGGTGG AGCATGCGGT GCTGCACCTG CTGTATGCCC GGTTCTGGCA CAAGGTGCTC TACGACCTGG GCCACGTCAC CTCCCGGGAG CCGTACCGGC GGCTGGTCAA CCAGGGCTAC ATCCAGGCCT ACGCCTACAC CGATCCGCGC GGCAGCTATG TGCCGGCCGC GGAGGTGATC GAGCGGGACG GCAGGTTCTA CTGGCCCGGG CCGGACGGCG AGATCGAGGT CAAACAGGAG TTCGGCAAGA TCGGCAAGAG CCTGAAGAAC TCCATCTCGC CCGACGAGAT CTGTGACGAG TACGGCGCGG ACACACTGCG GGTGTACGAG ATGTCGATGG GCCCGCTGGA GGCGTCACGG CCGTGGGCGC CCAAGGACGT CGTGGGGGCC TACCGGTTCC TGCAGCGGGT GTGGCGGCTG GTCGTCGACG AGGAGACCGG CGCCACCCGG GTGGCCGCGC ACGAGGCGGT GGACGAACAG ACACAGCGGT TGCTGCACAA GACGATCGCG GGGGTGTCGG AGGACTACGC GGCGCTGCGC AACAACACCG CGGCGGCCAA GCTGATCGAG TACACCAACC ATCTGACCAA GGAGAATGTC ACCGCCCGGG CGGCCATCGA GCCGTTGCTG CTGATGCTCG CCCCGCTGGC GCCGCATCTA GCCGAGGAGC TGTGGCACCG GGTGGGCAAC ACCGGATCGC TGGCGCACGG GCCGTTCCCG GAGGCCGACC CGCAGTATCT GGTCGAGGAC ACCGTCGAGA TGCCGGTGCA GGTCAACGGC AAGGTGCGCG GACACATCAC GGTCGCGGTG GACGCCGACA AGGCGACGCT GGAGGCCGCG GCGCTGGCCG ATGAGAAGGT GCAGGCTTTC CTGGCCGGCG CCACTCCGAA GAAGGTGATC GTGGTCCCCG GCAAGCTCGT CAACCTCGTC GTCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT