SSGCID: Target Page

MythA.17861.a

RNA polymerase beta subunit (Fragment) (Rv0667 ortholog)

CENTER ID:	MythA.17861.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.17861.a.B1.GE37653	full length		1	222

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	J9V7Q5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

GEKAREVRDT SLKVPHGESG KVIGIRVFSR EDDDELPAGV NELVRVYVAQ KRKISDGDKL AGRHGNKGVI GKILPVEDMP FLPDGTPVDI ILNTHGVPRR MNIGQILETH LGWVAKSGWD INLVGDKDLA SKLPEELHSA PPNSIVATPV FDGAQEEELQ ALLASTLPNR DGQQMVDNDG KAILYDGRSG EPFPYPVTVG YMYILKLHHL VDDKIHARST GP

NT Sequence

GGCGAGAAGG CCCGCGAGGT CCGCGACACC TCGCTGAAGG TGCCGCACGG CGAGTCCGGC AAGGTCATCG GCATCCGGGT GTTCTCCCGC GAGGACGACG ACGAGCTGCC GGCCGGTGTC AACGAACTGG TCCGCGTCTA CGTGGCCCAG AAGCGCAAGA TCTCCGACGG CGACAAGCTG GCCGGGCGCC ACGGCAACAA GGGCGTCATC GGCAAGATCC TGCCGGTCGA GGACATGCCG TTCCTGCCGG ACGGCACCCC GGTCGACATC ATCCTCAACA CCCACGGTGT GCCGCGGCGG ATGAACATCG GCCAGATCCT GGAGACCCAC CTCGGGTGGG TGGCCAAGAG CGGCTGGGAC ATCAACCTCG TCGGCGACAA GGACCTGGCG TCCAAGCTGC CCGAGGAGCT GCACTCGGCC CCGCCGAACA GCATCGTCGC CACCCCGGTG TTCGACGGTG CGCAGGAGGA GGAACTGCAG GCGCTGCTCG CCTCGACCCT GCCGAACCGC GACGGTCAGC AGATGGTCGA CAACGACGGC AAGGCGATCC TGTACGACGG GCGCTCCGGG GAACCGTTCC CGTACCCGGT GACGGTCGGC TACATGTACA TCCTCAAGCT GCACCACCTG GTGGACGACA AGATCCACGC CCGCTCCACC GGCCCG

Details for MythA.17861.a.B1.GE37653

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG EKAREVRDTS LKVPHGESGK VIGIRVFSRE DDDELPAGVN ELVRVYVAQK RKISDGDKLA GRHGNKGVIG KILPVEDMPF LPDGTPVDII LNTHGVPRRM NIGQILETHL GWVAKSGWDI NLVGDKDLAS KLPEELHSAP PNSIVATPVF DGAQEEELQA LLASTLPNRD GQQMVDNDGK AILYDGRSGE PFPYPVTVGY MYILKLHHLV DDKIHARSTG P

Validated NT Sequence

atcttactca cttacgggcc ggtggagcgg gcgtggatct tgtcgtccac caggtggtgc agcttgagga tgtacatgta gccgaccgtc accgggtacg ggaacggttc cccggagcgc ccgtcgtaca ggatcgcctt gccgtcgttg tcgaccatct gctgaccgtc gcggttcggc agggtcgagg cgagcagcgc ctgcagttcc tcctcctgcg caccgtcgaa caccggggtg gcgacgatgc tgttcggcgg ggccgagtgc agctcctcgg gcagcttgga cgccaggtcc ttgtcgccga cgaggttgat gtcccagccg ctcttggcca cccacccgag gtgggtctcc aggatctggc cgatgttcat ccgccgcggc acaccgtggg tgttgaggat gatgtcgacc ggggtgccgt ccggcaggaa cggcatgtcc tcgaccggca ggatcttgcc gatgacgccc ttgttgccgt ggcgcccggc cagcttgtcg ccgtcggaga tcttgcgctt ctgggccacg tagacgcgga ccagttcgtt gacaccggcc ggcagctcgt cgtcgtcctc gcgggagaac acccggatgc cgatgacctt gccggactcg ccgtgcggca ccttcagcga ggtgtcgcgg acctcgcggg ccttctcgcc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHGE KAREVRDTSL KVPHGESGKV IGIRVFSRED DDELPAGVNE LVRVYVAQKR KISDGDKLAG RHGNKGVIGK ILPVEDMPFL PDGTPVDIIL NTHGVPRRMN IGQILETHLG WVAKSGWDIN LVGDKDLASK LPEELHSAPP NSIVATPVFD GAQEEELQAL LASTLPNRDG QQMVDNDGKA ILYDGRSGEP FPYPVTVGYM YILKLHHLVD DKIHARSTGP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcgaga aggcccgcga ggtccgcgac acctcgctga aggtgccgca cggcgagtcc ggcaaggtca tcggcatccg ggtgttctcc cgcgaggacg acgacgagct gccggccggt gtcaacgaac tggtccgcgt ctacgtggcc cagaagcgca agatctccga cggcgacaag ctggccgggc gccacggcaa caagggcgtc atcggcaaga tcctgccggt cgaggacatg ccgttcctgc cggacggcac cccggtcgac atcatcctca acacccacgg tgtgccgcgg cggatgaaca tcggccagat cctggagacc cacctcgggt gggtggccaa gagcggctgg gacatcaacc tcgtcggcga caaggacctg gcgtccaagc tgcccgagga gctgcactcg gccccgccga acagcatcgt cgccaccccg gtgttcgacg gtgcgcagga ggaggaactg caggcgctgc tcgcctcgac cctgccgaac cgcgacggtc agcagatggt cgacaacgac ggcaaggcga tcctgtacga cgggcgctcc ggggaaccgt tcccgtaccc ggtgacggtc ggctacatgt acatcctcaa gctgcaccac ctggtggacg acaagatcca cgcccgctcc accggcccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat