SSGCID: Target Page

MythA.17274.a

DNA-directed RNA polymerase subunit beta' RpoC

CENTER ID:	MythA.17274.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.17274.a.B1.GE38639	full length		1	1315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2488
RefSeq:	WP_003926016.1
UniProt:	G7CKU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDVNFFDEL RIGLATADDI RRWSYGEVKK PETINYRTLK PEKDGLFCEK IFGPTRDWEC YCGKYKRVRF KGIICERCGV EVTRAKVRRE RMGHIELAAP VTHIWYFKGV PSRLGYLLDL APKDLEKIIY FAAYVITDVD EEMRHNELST LEAEMAVERK AVEDQRDLDL AERAKKLEQD LAELEAEGAK SDVRRKVRDA GEREMRQIRD RAQRELDRLD EIWNTFTKLA PKQLIVDEVL YRELVDRYGE YFQGGMGAEA IKKLIENFDI DAEAEALREV IRSGKGQKKL RALKRLKVVA AFQQSSNSPM GMVLDAVPVI PPELRPMVQL DGGRFATSDL NDLYRRVINR NNRLKRLIDL GAPEIIVNNE KRMLQESVDA LFDNGRRGRP VTGPGNRPLK SLSDLLKGKQ GRFRQNLLGK RVDYSGRSVI VVGPQLKLHQ CGLPKLMALE LFKPFVMKRL VDLNHAQNIK SAKRMVERQR PQVWDVLEEV IAEHPVLLNR APTLHRLGIQ AFEPLLVEGK AIQLHPLVCE AFNADFDGDQ MAVHLPLSAE AQAEARILML SSHNILSPAS GRPLAMPRLD MVTGLYYLTT MVPGDKGEYQ PATKDAPEQG VYSSPAEAIM AMDRGVLSVR AKIKVRLSDQ RPPADVEAEL FPDGWKPGDV WTTETTLGRV MFNELLPQDY PFVNEQMHKK VQARIVNDLA ERYPMIVVAQ TVDKLKDAGF YWATRSGVTV SMADVLVPPQ KKEILDRYEK EADGIEKKYQ RGALNYEERN EALVKIWQDA TEEVGQVLRD FYPEDNPIIM IVESGATGNF TQTRTLAGMK GLVTNPKGEF IPRPIKSSFR EGLTVLEYFI NTHGARKGLA DTALRTADSG YLTRRLVDVS QDVIVREHDC ETERGINVTL AERQPDGTLV RDQHIETSAY ARTLATDAVD KDGNVIVERG HDLGDPQIEA LLAAGITEVK VRSVLTCATG TGVCAMCYGR SMATGKLVDI GEAVGIVAAQ SIGEPGTQLT MRTFHQGGVG GDITGGLPRV QELFEARVPR NEAPIADVSG RIRLEEDDKF YKITIIPDDG GEEVVYDKLS RRQRLKVIKH PDGTERLLAD GDHVEVGQQL LEGSANPHEV LRVQGPREVQ IHLVKEVQEV YRAQGVSIHD KHIEVIVRQM LRRVTIIDSG STEFLPGSLT ERAEFEAENR RVVAEGGEPA AGRPVLMGIT KASLATDSWL SAASFQETTR VLTDAAINCR SDKLQGLKEN VIIGKLIPAG TGIPRYRNIQ VQPTEEARAA AYTIPTYEDQ YYSPDFGQAT GAAVPLDDYG YGDYR

NT Sequence

gtgctagacg tcaacttctt cgatgagctc cggatcggcc tggccaccgc cgacgacatc cgtcggtggt cctacggcga ggtgaagaag ccggagacca tcaactaccg cacgctgaag ccggagaagg atggcctgtt ctgcgagaag atcttcggac cgactcgcga ctgggagtgc tactgcggca agtacaagcg cgtccgcttc aagggaatca tctgtgagcg ctgcggcgtc gaggtgaccc gcgccaaggt gcgccgggag cggatgggcc acatcgaact ggccgcgccg gtcacccaca tctggtactt caagggtgtc ccgtcacggc tcggctacct gctcgacctg gcgcccaagg atctcgagaa gatcatctac ttcgccgcct acgtcatcac cgacgtcgac gaggagatgc gccacaacga gctgtccacg ctcgaagcgg agatggccgt cgagcgcaag gccgtcgagg accagcgtga cctcgacctg gccgagcggg ccaagaagct ggagcaggac ctcgccgaac tcgaggccga gggcgccaag tccgacgtcc gccgcaaggt gcgcgacgcc ggcgagcgcg agatgcgcca gatccgcgac cgggcccagc gcgagctgga ccggctcgac gagatctgga acaccttcac caagctggcg cccaagcagc tgatcgtcga cgaggtgctc taccgcgagc tggtcgaccg ctacggcgag tacttccagg gcgggatggg cgccgaggcg atcaagaagc tgatcgagaa cttcgacatc gacgctgagg ccgaggcgct gcgcgaggtg atccgcagcg gcaaggggca gaagaagctg cgggcgctga agcggctcaa ggtggtcgcg gcgttccagc agtcgagcaa ctcaccgatg ggcatggtgc tcgacgcggt gccggtgatc ccgccggagc tgcggccgat ggtgcagctc gacggtggcc ggttcgccac ctccgacctc aacgacctgt accgccgcgt catcaaccgc aacaaccggc tcaagcgact gatcgacctc ggcgcgcccg agatcatcgt caacaacgag aagcggatgc tgcaggagtc ggtggacgcg ctgttcgaca acggccggcg cggccgtccg gtcaccgggc cgggcaaccg tccgctcaag tcgctgtcgg atctgctcaa gggcaagcag ggccggttcc gccagaacct gctcggtaag cgcgtcgact actccggccg ttcggtgatc gtggtcggcc cgcagctcaa gctgcaccag tgcggtctgc ccaagctgat ggcgctggag ctgttcaagc cgttcgtgat gaagcggctg gtcgacctga accacgcgca gaacatcaag agcgccaagc ggatggtcga gcggcagcgt ccgcaggtgt gggacgtgct cgaagaggtc atcgccgagc acccggtgct gctgaaccgc gcgcccaccc tgcaccgcct gggcatccag gcgttcgagc cgctgctggt ggagggcaag gccatccagc tgcacccgct ggtgtgcgag gcgttcaacg ccgacttcga cggcgaccag atggccgtgc acctgccgct gagcgccgag gcgcaggccg aggcccgcat cctgatgctg tccagccaca acatcctgtc gccggcgtcc ggccgcccgc tggccatgcc gcgactggac atggtgaccg ggctgtacta cctgaccacg atggttccgg gggacaaggg cgagtaccag cccgccacca aggacgcccc ggagcagggt gtgtacagca gcccggccga ggcgatcatg gcgatggacc gcggcgtgct gagcgtccgg gccaagatca aggtgcggct gtcggatcag cggccgccgg ccgacgtcga ggccgagctg ttcccggacg gctggaagcc gggtgacgtc tggacgacgg agaccaccct gggccgggtg atgttcaacg agctgctgcc gcaggactat ccgttcgtca acgagcagat gcacaagaag gtccaggccc ggatcgtcaa cgacctggcc gagcgctacc cgatgatcgt ggtcgcgcag accgtcgaca agctcaagga cgccggcttc tactgggcga cccgctccgg tgtgacggtc tcgatggccg acgtgctggt gccgccgcag aagaaggaga tcctggaccg ctacgagaag gaagcggacg ggatcgagaa gaagtaccag cgcggtgcgc tcaactacga ggaacgcaac gaggccctgg tcaagatctg gcaggacgcc accgaagagg tggggcaggt gctgcgggac ttctaccccg aggacaaccc gatcatcatg atcgtggagt ccggcgccac gggtaacttc acccagaccc ggaccctggc cggcatgaag ggcctggtga ccaacccgaa gggtgagttc atcccgcggc cgatcaagtc ctcgttccgt gagggcctga ccgtgctgga gtacttcatc aacacccacg gcgcccgtaa gggtctggcg gacaccgcgc tgcggaccgc cgactcgggt tacctgactc gtcgtctggt ggacgtgtcg caggacgtca tcgtgcgcga gcacgactgc gagaccgagc gcggcatcaa cgtcacgctc gccgagcggc agccggacgg cacgctggtc cgcgatcagc acatcgagac ctcggcctac gcccggacgc tggccaccga cgcggtcgac aaggacggca acgtcatcgt cgaacggggc cacgacctgg gcgacccgca gatcgaggcg ctgctggcgg ccggcatcac cgaggtcaag gtgcggtcgg tgctcacctg cgccaccggc accggggtgt gcgcgatgtg ctacggccgt tcgatggcga ccggcaagct ggtcgacatc ggcgaggcgg tcggcatcgt cgccgcccag tccatcggtg agcccggtac ccagctgacc atgcggacgt tccaccaggg tggtgtcggc ggtgacatca ccggtggtct gccgcgggtg caggagctgt tcgaggcccg ggtgccgcgc aacgaggcgc cgatcgccga cgtcagcggc cggatccggc tcgaagagga cgacaagttc tacaagatca ccatcatccc cgacgacggg ggcgaggagg tcgtctacga caagctctcc cgtcgtcagc ggttgaaggt catcaagcat cccgacggga cggagcggct gctcgccgac ggcgaccacg tcgaggtggg ccagcagctg ctcgaaggtt cggccaaccc gcacgaggtg ctgcgtgtgc aggggccccg cgaggtgcag atccacctcg tcaaggaggt ccaggaggtc taccgtgctc agggcgtgtc gatccacgac aagcacatcg aggtgatcgt ccggcagatg ctgcgccgcg tgaccatcat cgactcgggc tcgacggagt ttctgcccgg gtcgctgacc gagcgggcgg agttcgaggc cgagaaccgt cgggtggtgg ccgagggcgg cgagcccgcg gccggtcgtc cggtgctgat gggtatcacc aaggcgtcgc tggccaccga ttcgtggctc agtgcggcgt cgttccagga gaccacgcgc gtgctgaccg atgcggcgat caactgccgc agcgacaagc tgcagggtct gaaggagaac gtgatcatcg gcaagctgat cccggccggt accggtattc cgcggtaccg caacatccag gtgcagccga ccgaggaggc ccgggccgct gcgtacacga tcccgaccta cgaggatcag tactacagcc ccgacttcgg tcaggccacc ggtgccgcgg ttccgctgga cgactacggc tacggcgact accgctag

Details for MythA.17274.a.B1.GE38639

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	148kDa
OBSERVED MW:	148kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML DVNFFDELRI GLATADDIRR WSYGEVKKPE TINYRTLKPE KDGLFCEKIF GPTRDWECYC GKYKRVRFKG IICERCGVEV TRAKVRRERM GHIELAAPVT HIWYFKGVPS RLGYLLDLAP KDLEKIIYFA AYVITDVDEE MRHNELSTLE AEMAVERKAV EDQRDLDLAE RAKKLEQDLA ELEAEGAKSD VRRKVRDAGE REMRQIRDRA QRELDRLDEI WNTFTKLAPK QLIVDEVLYR ELVDRYGEYF QGGMGAEAIK KLIENFDIDA EAEALREVIR SGKGQKKLRA LKRLKVVAAF QQSSNSPMGM VLDAVPVIPP ELRPMVQLDG GRFATSDLND LYRRVINRNN RLKRLIDLGA PEIIVNNEKR MLQESVDALF DNGRRGRPVT GPGNRPLKSL SDLLKGKQGR FRQNLLGKRV DYSGRSVIVV GPQLKLHQCG LPKLMALELF KPFVMKRLVD LNHAQNIKSA KRMVERQRPQ VWDVLEEVIA EHPVLLNRAP TLHRLGIQAF EPLLVEGKAI QLHPLVCEAF NADFDGDQMA VHLPLSAEAQ AEARILMLSS HNILSPASGR PLAMPRLDMV TGLYYLTTMV PGDKGEYQPA TKDAPEQGVY SSPAEAIMAM DRGVLSVRAK IKVRLSDQRP PADVEAELFP DGWKPGDVWT TETTLGRVMF NELLPQDYPF VNEQMHKKVQ ARIVNDLAER YPMIVVAQTV DKLKDAGFYW ATRSGVTVSM ADVLVPPQKK EILDRYEKEA DGIEKKYQRG ALNYEERNEA LVKIWQDATE EVGQVLRDFY PEDNPIIMIV ESGATGNFTQ TRTLAGMKGL VTNPKGEFIP RPIKSSFREG LTVLEYFINT HGARKGLADT ALRTADSGYL TRRLVDVSQD VIVREHDCET ERGINVTLAE RQPDGTLVRD QHIETSAYAR TLATDAVDKD GNVIVERGHD LGDPQIEALL AAGITEVKVR SVLTCATGTG VCAMCYGRSM ATGKLVDIGE AVGIVAAQSI GEPGTQLTMR TFHQGGVGGD ITGGLPRVQE LFEARVPRNE APIADVSGRI RLEEDDKFYK ITIIPDDGGE EVVYDKLSRR QRLKVIKHPD GTERLLADGD HVEVGQQLLE GSANPHEVLR VQGPREVQIH LVKEVQEVYR AQGVSIHDKH IEVIVRQMLR RVTIIDSGST EFLPGSLTER AEFEAENRRV VAEGGEPAAG RPVLMGITKA SLATDSWLSA ASFQETTRVL TDAAINCRSD KLQGLKENVI IGKLIPAGTG IPRYRNIQVQ PTEEARAAAY TIPTYEDQYY SPDFGQATGA AVPLDDYGYG DYR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctag acgtcaactt cttcgatgag ctccggatcg gcctggccac cgccgacgac atccgtcggt ggtcctacgg cgaggtgaag aagccggaga ccatcaacta ccgcacgctg aagccggaga aggatggcct gttctgcgag aagatcttcg gaccgactcg cgactgggag tgctactgcg gcaagtacaa gcgcgtccgc ttcaagggaa tcatctgtga gcgctgcggc gtcgaggtga cccgcgccaa ggtgcgccgg gagcggatgg gccacatcga actggccgcg ccggtcaccc acatctggta cttcaagggt gtcccgtcac ggctcggcta cctgctcgac ctggcgccca aggatctcga gaagatcatc tacttcgccg cctacgtcat caccgacgtc gacgaggaga tgcgccacaa cgagctgtcc acgctcgaag cggagatggc cgtcgagcgc aaggccgtcg aggaccagcg tgacctcgac ctggccgagc gggccaagaa gctggagcag gacctcgccg aactcgaggc cgagggcgcc aagtccgacg tccgccgcaa ggtgcgcgac gccggcgagc gcgagatgcg ccagatccgc gaccgggccc agcgcgagct ggaccggctc gacgagatct ggaacacctt caccaagctg gcgcccaagc agctgatcgt cgacgaggtg ctctaccgcg agctggtcga ccgctacggc gagtacttcc agggcgggat gggcgccgag gcgatcaaga agctgatcga gaacttcgac atcgacgctg aggccgaggc gctgcgcgag gtgatccgca gcggcaaggg gcagaagaag ctgcgggcgc tgaagcggct caaggtggtc gcggcgttcc agcagtcgag caactcaccg atgggcatgg tgctcgacgc ggtgccggtg atcccgccgg agctgcggcc gatggtgcag ctcgacggtg gccggttcgc cacctccgac ctcaacgacc tgtaccgccg cgtcatcaac cgcaacaacc ggctcaagcg actgatcgac ctcggcgcgc ccgagatcat cgtcaacaac gagaagcgga tgctgcagga gtcggtggac gcgctgttcg acaacggccg gcgcggccgt ccggtcaccg ggccgggcaa ccgtccgctc aagtcgctgt cggatctgct caagggcaag cagggccggt tccgccagaa cctgctcggt aagcgcgtcg actactccgg ccgttcggtg atcgtggtcg gcccgcagct caagctgcac cagtgcggtc tgcccaagct gatggcgctg gagctgttca agccgttcgt gatgaagcgg ctggtcgacc tgaaccacgc gcagaacatc aagagcgcca agcggatggt cgagcggcag cgtccgcagg tgtgggacgt gctcgaagag gtcatcgccg agcacccggt gctgctgaac cgcgcgccca ccctgcaccg cctgggcatc caggcgttcg agccgctgct ggtggagggc aaggccatcc agctgcaccc gctggtgtgc gaggcgttca acgccgactt cgacggcgac cagatggccg tgcacctgcc gctgagcgcc gaggcgcagg ccgaggcccg catcctgatg ctgtccagcc acaacatcct gtcgccggcg tccggccgcc cgctggccat gccgcgactg gacatggtga ccgggctgta ctacctgacc acgatggttc cgggggacaa gggcgagtac cagcccgcca ccaaggacgc cccggagcag ggtgtgtaca gcagcccggc cgaggcgatc atggcgatgg accgcggcgt gctgagcgtc cgggccaaga tcaaggtgcg gctgtcggat cagcggccgc cggccgacgt cgaggccgag ctgttcccgg acggctggaa gccgggtgac gtctggacga cggagaccac cctgggccgg gtgatgttca acgagctgct gccgcaggac tatccgttcg tcaacgagca gatgcacaag aaggtccagg cccggatcgt caacgacctg gccgagcgct acccgatgat cgtggtcgcg cagaccgtcg acaagctcaa ggacgccggc ttctactggg cgacccgctc cggtgtgacg gtctcgatgg ccgacgtgct ggtgccgccg cagaagaagg agatcctgga ccgctacgag aaggaagcgg acgggatcga gaagaagtac cagcgcggtg cgctcaacta cgaggaacgc aacgaggccc tggtcaagat ctggcaggac gccaccgaag aggtggggca ggtgctgcgg gacttctacc ccgaggacaa cccgatcatc atgatcgtgg agtccggcgc cacgggtaac ttcacccaga cccggaccct ggccggcatg aagggcctgg tgaccaaccc gaagggtgag ttcatcccgc ggccgatcaa gtcctcgttc cgtgagggcc tgaccgtgct ggagtacttc atcaacaccc acggcgcccg taagggtctg gcggacaccg cgctgcggac cgccgactcg ggttacctga ctcgtcgtct ggtggacgtg tcgcaggacg tcatcgtgcg cgagcacgac tgcgagaccg agcgcggcat caacgtcacg ctcgccgagc ggcagccgga cggcacgctg gtccgcgatc agcacatcga gacctcggcc tacgcccgga cgctggccac cgacgcggtc gacaaggacg gcaacgtcat cgtcgaacgg ggccacgacc tgggcgaccc gcagatcgag gcgctgctgg cggccggcat caccgaggtc aaggtgcggt cggtgctcac ctgcgccacc ggcaccgggg tgtgcgcgat gtgctacggc cgttcgatgg cgaccggcaa gctggtcgac atcggcgagg cggtcggcat cgtcgccgcc cagtccatcg gtgagcccgg tacccagctg accatgcgga cgttccacca gggtggtgtc ggcggtgaca tcaccggtgg tctgccgcgg gtgcaggagc tgttcgaggc ccgggtgccg cgcaacgagg cgccgatcgc cgacgtcagc ggccggatcc ggctcgaaga ggacgacaag ttctacaaga tcaccatcat ccccgacgac gggggcgagg aggtcgtcta cgacaagctc tcccgtcgtc agcggttgaa ggtcatcaag catcccgacg ggacggagcg gctgctcgcc gacggcgacc acgtcgaggt gggccagcag ctgctcgaag gttcggccaa cccgcacgag gtgctgcgtg tgcaggggcc ccgcgaggtg cagatccacc tcgtcaagga ggtccaggag gtctaccgtg ctcagggcgt gtcgatccac gacaagcaca tcgaggtgat cgtccggcag atgctgcgcc gcgtgaccat catcgactcg ggctcgacgg agtttctgcc cgggtcgctg accgagcggg cggagttcga ggccgagaac cgtcgggtgg tggccgaggg cggcgagccc gcggccggtc gtccggtgct gatgggtatc accaaggcgt cgctggccac cgattcgtgg ctcagtgcgg cgtcgttcca ggagaccacg cgcgtgctga ccgatgcggc gatcaactgc cgcagcgaca agctgcaggg tctgaaggag aacgtgatca tcggcaagct gatcccggcc ggtaccggta ttccgcggta ccgcaacatc caggtgcagc cgaccgagga ggcccgggcc gctgcgtaca cgatcccgac ctacgaggat cagtactaca gccccgactt cggtcaggcc accggtgccg cggttccgct ggacgactac ggctacggcg actaccgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat