SSGCID: Target Page

MythA.17208.a

RNA polymerase sigma factor

CENTER ID:	MythA.17208.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.17208.a.B1.GE39184	full length		1	212

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.4445
UniProt:	G7CNA0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDIEGTTPI RSEESDAELT ARFERDAIPL LDQLYGGALR MTRNPADAED LLQETMVKAY QGFRSFREGT NLKAWLYRIL TNTYINNYRK RQRQPAVYPT EEFTDWQLAS TAEHSSTGLR SAEVEALEAL PDTEIKAALQ ELPEDFRIAV YLADVEGFPY KEIAEIMDTP IGTVMSRLHR GRRQLRSLLA DVARERGIIR DQQAGTPEEV SS

NT Sequence

atgaccgaca tcgagggcac cacgccaatc aggtccgaag agagcgacgc cgagctgacg gctcgattcg agcgagacgc catcccgttg ctcgaccagc tgtacggtgg cgcgttgcgg atgacgcgca atccggccga tgcggaagat ctccttcagg agaccatggt caaggcctac cagggcttcc gctcgttccg ggaggggacg aacctcaagg cgtggctgta ccggattctg accaacacct acatcaacaa ctaccgaaag aggcagcgcc agcccgcggt gtatccgacc gaggagttca ccgactggca gctggccagc actgcggagc attcctccac cgggctgcgg tcagcggagg tggaggccct ggaggcgctg cctgacaccg agatcaaggc tgcgctgcag gaactccccg aggatttccg catcgcggtg tacctcgcgg acgtcgaagg cttcccatac aaggaaatcg ccgagatcat ggacacgccg atcggaacgg tgatgtcccg gttgcaccgg ggtcgccgcc aattgcggtc cctgctggcc gacgtggccc gggagcgtgg catcatccgt gatcagcagg ctggtacccc tgaggaggtg tcgtcgtga

Details for MythA.17208.a.B1.GE39184

HARVESTED ON:	data unavailable
SEQUENCED ON:	5/6/2015
EXPECTED MW:	25kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DIEGTTPIRS EESDAELTAR FERDAIPLLD QLYGGALRMT RNPADAEDLL QETMVKAYQG FRSFREGTNL KAWLYRILTN TYINNYRKRQ RQPAVYPTEE FTDWQLASTA EHSSTGLRSA EVEALEALPD TEIKAALQEL PEDFRIAVYL ADVEGFPYKE IAEIMDTPIG TVMSRLHRGR RQLRSLLADV ARERGIIRDQ QAGTPEEVSS

Validated NT Sequence

atcttactca cttacgacga cacctcctca ggggtaccag cctgctgatc acggatgatg ccacgctccc gggccacgtc ggccagcagg gaccgcaatt ggcggcgacc ccggtgcaac cgggacatca ccgttccgat cggcgtgtcc atgatctcgg cgatttcctt gtatgggaag ccttcgacgt ccgcgaggta caccgcgatg cggaaatcct cggggagttc ctgcagcgca gccttgatct cggtgtcagg cagcgcctcc agggcctcca cctccgctga ccgcagcccg gtggaggaat gctccgcagt gctggccagc tgccagtcgg tgaactcctc ggtcggatac accgcgggct ggcgctgcct ctttcggtag ttgttgatgt aggtgttggt cagaatccgg tacagccacg ccttgaggtt cgtcccctcc cggaacgagc ggaagccctg gtaggccttg accatggtct cctgaaggag atcttccgca tcggccggat tgcgcgtcat ccgcaacgcg ccaccgtaca gctggtcgag caacgggatg gcgtctcgct cgaatcgagc cgtcagctcg gcgtcgctct cttcggacct gattggcgtg gtgccctcga tgtcggtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acatcgaggg caccacgcca atcaggtccg aagagagcga cgccgagctg acggctcgat tcgagcgaga cgccatcccg ttgctcgacc agctgtacgg tggcgcgttg cggatgacgc gcaatccggc cgatgcggaa gatctccttc aggagaccat ggtcaaggcc taccagggct tccgctcgtt ccgggagggg acgaacctca aggcgtggct gtaccggatt ctgaccaaca cctacatcaa caactaccga aagaggcagc gccagcccgc ggtgtatccg accgaggagt tcaccgactg gcagctggcc agcactgcgg agcattcctc caccgggctg cggtcagcgg aggtggaggc cctggaggcg ctgcctgaca ccgagatcaa ggctgcgctg caggaactcc ccgaggattt ccgcatcgcg gtgtacctcg cggacgtcga aggcttccca tacaaggaaa tcgccgagat catggacacg ccgatcggaa cggtgatgtc ccggttgcac cggggtcgcc gccaattgcg gtccctgctg gccgacgtgg cccgggagcg tggcatcatc cgtgatcagc aggctggtac ccctgaggag gtgtcgtcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat