SSGCID: Target Page

MythA.11416.a

Chromosome segregation ATPase (Rv3918c ortholog)

CENTER ID:	MythA.11416.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.11416.a.B1.GE37649	full length		1	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.11416.a.B1.PW37470	full length		1	319

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2104
UniProt:	G7CIN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRPGPAAADA WAADTVDTPI GAEAERAVRL LHSARHGQLP RPARQRVFTI ANQKGGVGKT TTAVNVAAAL ALQGLKTLVI DLDPQGNAST ALGIEHRPGT PSSYEVLIGE IELETALQRS PHNERLYCVP ATIDLAGAEI ELVSMVAREG RLRGALAELK NYDFDYVFID CPPSLGLLTI NALVAAPEVL IPIQCEYYAL EGVGQLLRNI QMVKAHLNPE LEVTTVLLTM YDGRTKLADQ VAADVRAHFG DKVLRTVIPR SVKVSEAPGY GMTILDYDAG SRGAMSYLDA SREIAERGLQ QGRQAQQPGT GQPNSKDHA

NT Sequence

gtgcgccccg ggcccgccgc ggccgacgcg tgggcagccg acaccgtgga caccccgatc ggcgcggagg cggaacgtgc cgtgcggttg ctgcattcgg cccggcacgg tcagctgccc cgaccggccc gccagcgggt gttcaccatc gcgaaccaga agggcggggt cggtaagacc acgaccgcgg tgaacgtcgc ggcggcgctc gccctccagg gcctcaagac cctggtgatc gatctcgatc cgcagggcaa tgccagcacc gcgctcggca tcgaacaccg tccggggaca ccgtcgtcct atgaggtgtt gatcggcgag atcgagctgg agaccgcgct gcagcgtagc ccccacaacg aacggctcta ctgtgtaccg gccaccatcg acctcgccgg tgccgagatc gagttggtga gcatggtcgc gcgcgagggc cggctgcgcg gtgcgctggc cgagttgaag aactacgact tcgattacgt cttcatcgac tgcccaccgt cgctcggtct gctcaccatc aacgcgttgg tggcggcgcc ggaggtgctg atcccgatcc agtgcgagta ttacgcgctg gagggggtgg ggcagttgct ccggaacatc cagatggtca aggcgcatct gaatcccgaa ctggaagtca ccaccgtgct gctgaccatg tacgacggcc gcacgaaact ggccgatcag gtcgccgccg acgtgcgggc ccatttcggt gacaaggtgc tccggacggt gattccccgc agcgtgaagg tgtccgaggc acccggctac ggcatgacga ttctcgatta cgacgcgggc tcccgcgggg cgatgagcta tctcgacgcc agccgggaga tcgccgaacg cggcctgcag cagggtcggc aggcacagca acccgggact ggtcaaccga attcgaagga tcacgcatga

Details for MythA.11416.a.B1.GE37649

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MVRPGPAAAD AWAADTVDTP IGAEAERAVR LLHSARHGQL PRPARQRVFT IANQKGGVGK TTTAVNVAAA LALQGLKTLV IDLDPQGNAS TALGIEHRPG TPSSYEVLIG EIELETALQR SPHNERLYCV PATIDLAGAE IELVSMVARE GRLRGALAEL KNYDFDYVFI DCPPSLGLLT INALVAAPEV LIPIQCEYYA LEGVGQLLRN IQMVKAHLNP ELEVTTVLLT MYDGRTKLAD QVAADVRAHF GDKVLRTVIP RSVKVSEAPG YGMTILDYDA GSRGAMSYLD ASREIAERGL QQGRQAQQPG TGQPNSKDHA

Validated NT Sequence

cttactcact tatgcgtgat ccttcgaatt cggttgacca gtcccgggtt gctgtgcctg ccgaccctgc tgcaggccgc gttcggcgat ctcccggctg gcgtcgagat agctcatcgc cccgcgggag cccgcgtcgt aatcgagaat cgtcatgccg tagccgggtg cctcggacac cttcacgctg cggggaatca ccgtccggag caccttgtca ccgaaatggg cccgcacgtc ggcggcgacc tgatcggcca gtttcgtgcg gccgtcgtac atggtcagca gcacggtggt gacttccagt tcgggattca gatgcgcctt gaccatctgg atgttccgga gcaactgccc caccccctcc agcgcgtaat actcgcactg gatcgggatc agcacctccg gcgccgccac caacgcgttg atggtgagca gaccgagcga cggtgggcag tcgatgaaga cgtaatcgaa gtcgtagttc ttcaactcgg ccagcgcacc gcgcagccgg ccctcgcgcg cgaccatgct caccaactcg atctcggcac cggcgaggtc gatggtggcc ggtacacagt agagccgttc gttgtggggg ctacgctgca gcgcggtctc cagctcgatc tcgccgatca acacctcata ggacgacggt gtccccggac ggtgttcgat gccgagcgcg gtgctggcat tgccctgcgg atcgagatcg atcaccaggg tcttgaggcc ctggagggcg agcgccgccg cgacgttcac cgcggtcgtg gtcttaccga ccccgccctt ctggttcgcg atggtgaaca cccgctggcg ggccggtcgg ggcagctgac cgtgccgggc cgaatgcagc aaccgcacgg cacgttccgc ctccgcgccg atcggggtgt ccacggtgtc ggctgcccac gcgtcggccg cggcgggccc ggggcgcacc atatggtggt ggtggtggtg

Expected Protein Sequence

MAHHHHHHMR PGPAAADAWA ADTVDTPIGA EAERAVRLLH SARHGQLPRP ARQRVFTIAN QKGGVGKTTT AVNVAAALAL QGLKTLVIDL DPQGNASTAL GIEHRPGTPS SYEVLIGEIE LETALQRSPH NERLYCVPAT IDLAGAEIEL VSMVAREGRL RGALAELKNY DFDYVFIDCP PSLGLLTINA LVAAPEVLIP IQCEYYALEG VGQLLRNIQM VKAHLNPELE VTTVLLTMYD GRTKLADQVA ADVRAHFGDK VLRTVIPRSV KVSEAPGYGM TILDYDAGSR GAMSYLDASR EIAERGLQQG RQAQQPGTGQ PNSKDHA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcc ccgggcccgc cgcggccgac gcgtgggcag ccgacaccgt ggacaccccg atcggcgcgg aggcggaacg tgccgtgcgg ttgctgcatt cggcccggca cggtcagctg ccccgaccgg cccgccagcg ggtgttcacc atcgcgaacc agaagggcgg ggtcggtaag accacgaccg cggtgaacgt cgcggcggcg ctcgccctcc agggcctcaa gaccctggtg atcgatctcg atccgcaggg caatgccagc accgcgctcg gcatcgaaca ccgtccgggg acaccgtcgt cctatgaggt gttgatcggc gagatcgagc tggagaccgc gctgcagcgt agcccccaca acgaacggct ctactgtgta ccggccacca tcgacctcgc cggtgccgag atcgagttgg tgagcatggt cgcgcgcgag ggccggctgc gcggtgcgct ggccgagttg aagaactacg acttcgatta cgtcttcatc gactgcccac cgtcgctcgg tctgctcacc atcaacgcgt tggtggcggc gccggaggtg ctgatcccga tccagtgcga gtattacgcg ctggaggggg tggggcagtt gctccggaac atccagatgg tcaaggcgca tctgaatccc gaactggaag tcaccaccgt gctgctgacc atgtacgacg gccgcacgaa actggccgat caggtcgccg ccgacgtgcg ggcccatttc ggtgacaagg tgctccggac ggtgattccc cgcagcgtga aggtgtccga ggcacccggc tacggcatga cgattctcga ttacgacgcg ggctcccgcg gggcgatgag ctatctcgac gccagccggg agatcgccga acgcggcctg cagcagggtc ggcaggcaca gcaacccggg actggtcaac cgaattcgaa ggatcacgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for MythA.11416.a.B1.PW37470

PURIFICATION DATe:	4/1/2014
CONCENTRATION:	6.6mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCGCC CCGGGCCCGC CGCGGCCGAC GCGTGGGCAG CCGACACCGT GGACACCCCG ATCGGCGCGG AGGCGGAACG TGCCGTGCGG TTGCTGCATT CGGCCCGGCA CGGTCAGCTG CCCCGACCGG CCCGCCAGCG GGTGTTCACC ATCGCGAACC AGAAGGGCGG GGTCGGTAAG ACCACGACCG CGGTGAACGT CGCGGCGGCG CTCGCCCTCC AGGGCCTCAA GACCCTGGTG ATCGATCTCG ATCCGCAGGG CAATGCCAGC ACCGCGCTCG GCATCGAACA CCGTCCGGGG ACACCGTCGT CCTATGAGGT GTTGATCGGC GAGATCGAGC TGGAGACCGC GCTGCAGCGT AGCCCCCACA ACGAACGGCT CTACTGTGTA CCGGCCACCA TCGACCTCGC CGGTGCCGAG ATCGAGTTGG TGAGCATGGT CGCGCGCGAG GGCCGGCTGC GCGGTGCGCT GGCCGAGTTG AAGAACTACG ACTTCGATTA CGTCTTCATC GACTGCCCAC CGTCGCTCGG TCTGCTCACC ATCAACGCGT TGGTGGCGGC GCCGGAGGTG CTGATCCCGA TCCAGTGCGA GTATTACGCG CTGGAGGGGG TGGGGCAGTT GCTCCGGAAC ATCCAGATGG TCAAGGCGCA TCTGAATCCC GAACTGGAAG TCACCACCGT GCTGCTGACC ATGTACGACG GCCGCACGAA ACTGGCCGAT CAGGTCGCCG CCGACGTGCG GGCCCATTTC GGTGACAAGG TGCTCCGGAC GGTGATTCCC CGCAGCGTGA AGGTGTCCGA GGCACCCGGC TACGGCATGA CGATTCTCGA TTACGACGCG GGCTCCCGCG GGGCGATGAG CTATCTCGAC GCCAGCCGGG AGATCGCCGA ACGCGGCCTG CAGCAGGGTC GGCAGGCACA GCAACCCGGG ACTGGTCAAC CGAATTCGAA GGATCACGCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT