MythA.10520.a

Hydrolase (A0QYW5 homolog)(putative beta-lactamase)

CENTER ID: MythA.10520.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MythA.10520.a.A1.GE29000 Full length( MythA.10520.a ) 1 225
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1078020.3.peg.394
RefSeq: WP_003923830.1
UniProt: G7CBR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVVAEEYTG HVEPQTAARR TLPGASIIKV SVGPMDNNAY LITCSRTGET LLIDAANDAE ILLDLIERYA PKLSLIVTSH QHFDHWQALE KVVAATGAPT AAHHLDAEPL PVPPDRILSG GETITIGDLH FDVIHLRGHT PGSVALALSG PPAGHTTHLF TGDCLFPGGV GKTWEPGAFE QLLDDVITRV FDVYPDSTVV YPGHGDDTTL GAERPHLGEW RERGW
NT Sequence
ATGACAGTCG TCGCCGAGGA GTACACCGGA CACGTCGAAC CTCAGACCGC GGCACGGCGG ACGTTGCCCG GCGCATCGAT CATCAAGGTG TCGGTCGGCC CCATGGACAA CAACGCCTAC CTGATCACCT GTTCCCGCAC CGGCGAAACC CTGCTGATCG ACGCCGCCAA CGACGCCGAG ATCCTGCTCG ACCTCATCGA ACGGTACGCC CCGAAACTAT CGCTGATCGT CACCAGCCAC CAGCACTTCG ACCACTGGCA GGCGCTGGAG AAGGTCGTCG CGGCCACCGG TGCCCCGACC GCGGCGCACC ACCTCGACGC CGAACCGCTG CCGGTGCCGC CGGACCGCAT CCTCTCCGGC GGTGAGACGA TCACGATCGG TGATCTGCAC TTCGACGTGA TCCACCTGCG CGGACACACG CCGGGGTCGG TCGCGCTGGC GCTGAGCGGC CCGCCCGCCG GGCATACCAC CCACCTGTTC ACCGGCGACT GCCTGTTCCC GGGCGGGGTC GGCAAGACCT GGGAACCGGG CGCCTTCGAA CAGTTGCTCG ACGATGTCAT CACCCGGGTG TTCGACGTCT ATCCGGACTC GACGGTGGTG TATCCGGGCC ACGGCGACGA CACCACGCTG GGCGCCGAAC GCCCACACCT CGGAGAATGG CGGGAGCGGG GCTGG
Details for MythA.10520.a.A1.GE29000
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVAEEYT GHVEPQTAAR RTLPGASIIK VSVGPMDNNA YLITCSRTGE TLLIDAANDA EILLDLIERY APKLSLIVTS HQHFDHWQAL EKVVAATGAP TAAHHLDAEP LPVPPDRILS GGETITIGDL HFDVIHLRGH TPGSVALALS GPPAGHTTHL FTGDCLFPGG VGKTWEPGAF EQLLDDVITR VFDVYPDSTV VYPGHGDDTT LGAERPHLGE WRERGW
Validated NT Sequence
ttattaccag ccccgctccc gccattctcc gaggtgtggg cgttcggcgc ccagcgtggt gtcgtcgccg tggcccggat acaccaccgt cgagtccgga tagacgtcga acacccgggt gatgacatcg tcgagcaact gttcgaaggc gcccggttcc caggtcttgc cgaccccgcc cgggaacagg cagtcgccgg tgaacaggtg ggtggtatgc ccggcgggcg ggccgctcag cgccagcgcg accgaccccg gcgtgtgtcc gcgcaggtgg atcacgtcga agtgcagatc accgatcgtg atcgtctcac cgccggagag gatgcggtcc ggcggcaccg gcagcggttc ggcgtcgagg tggtgcgccg cggtcggggc accggtggcc gcgacgacct tctccagcgc ctgccagtgg tcgaagtgct ggtggctggt gacgatcagc gatagtttcg gggcgtaccg ttcgatgagg tcgagcagga tctcggcgtc gttggcggcg tcgatcagca gggtttcgcc ggtgcgggaa caggtgatca ggtaggcgtt gttgtccatg gggccgaccg acaccttgat gatcgatgcg ccgggcaacg tccgccgtgc cgcggtctga ggttcgacgt gtccggtgta ctcctcggcg acgactgtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVAEEYT GHVEPQTAAR RTLPGASIIK VSVGPMDNNA YLITCSRTGE TLLIDAANDA EILLDLIERY APKLSLIVTS HQHFDHWQAL EKVVAATGAP TAAHHLDAEP LPVPPDRILS GGETITIGDL HFDVIHLRGH TPGSVALALS GPPAGHTTHL FTGDCLFPGG VGKTWEPGAF EQLLDDVITR VFDVYPDSTV VYPGHGDDTT LGAERPHLGE WRERGW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagtcgtc gccgaggagt acaccggaca cgtcgaacct cagaccgcgg cacggcggac gttgcccggc gcatcgatca tcaaggtgtc ggtcggcccc atggacaaca acgcctacct gatcacctgt tcccgcaccg gcgaaaccct gctgatcgac gccgccaacg acgccgagat cctgctcgac ctcatcgaac ggtacgcccc gaaactatcg ctgatcgtca ccagccacca gcacttcgac cactggcagg cgctggagaa ggtcgtcgcg gccaccggtg ccccgaccgc ggcgcaccac ctcgacgccg aaccgctgcc ggtgccgccg gaccgcatcc tctccggcgg tgagacgatc acgatcggtg atctgcactt cgacgtgatc cacctgcgcg gacacacgcc ggggtcggtc gcgctggcgc tgagcggccc gcccgccggg cataccaccc acctgttcac cggcgactgc ctgttcccgg gcggggtcgg caagacctgg gaaccgggcg ccttcgaaca gttgctcgac gatgtcatca cccgggtgtt cgacgtctat ccggactcga cggtggtgta tccgggccac ggcgacgaca ccacgctggg cgccgaacgc ccacacctcg gagaatggcg ggagcggggc tggaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa