MythA.01613.k

Acyl-CoA dehydrogenase (A0QTN2 homolog)

CENTER ID: MythA.01613.k
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01613.k.A1.GE28803 Full length( MythA.01613.k ) 1 463
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.998
RefSeq: WP_003924481.1
UniProt: G7CDF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRWPRRIAR TPTSTSPPPT SRCTAASASP GSIRRTCICG GPAPTRNCSA IRPGTVSATC SGLEPDMSEL TDDQVRTEVR EWLAANWKPG MDRAEWARLV FEAGWAVPXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XPGTGHDRAN LFACTLHDLG TEEQKRRLIP PSIRGETRWC LLYSEPGAGS DLAGLRTRAE RDGDEWVING QKVWTSFAQT ADYGLLVART DWDVPKHKGI SFFMLPMKQP GIEVRPIHQI TGESEFNEVF ITDARCPAEN MVGRPGEGWS VLQVALGYER RLMGDLARTS KASRKPKDDP AQQRNALVEM AREAGRLDDP YIRQRIARMD EFAAVNRWNT QRSKATRDKV ESLTLQALGK IAMSRILHET ARVQTEIVGA ESMLAGPDHP IGDGVTFRTL NAYFTSIGGG TDQVQRNIVG ERVLGLPKEP DPYRDQPFRD LPH
NT Sequence
ATCTCGCGCT GGCCCAGGCG TATTGCTCGG ACGCCTACGT CTACGTCGCC GCCACCAACA TCCAGGTGCA CGGCGGCATC GGCTTCACCT GGGAGCATCC GGCGCACCTG TATCTGCGGC GGGCCCGCAC CGACGCGCAA CTGTTCGGCG ATCCGGCCTG GCACCGTGAG CGCTACCTGC AGCGGATTGG AGCCTGACAT GAGCGAGTTG ACCGACGATC AGGTCCGGAC CGAGGTCCGC GAGTGGCTGG CCGCGAACTG GAAACCCGGG ATGGACCGCG CCGAATGGGC CCGGCTGGTG TTCGAGGCCG GCTGGGCGGT TCCCNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCCGGCA CCGGCCACGA CCGGGCCAAC CTGTTCGCCT GCACCCTGCA CGATCTGGGC ACCGAGGAGC AGAAGCGGCG GCTGATCCCG CCGTCCATCC GCGGTGAGAC CCGGTGGTGT CTGCTCTACA GCGAACCGGG CGCCGGATCG GACCTCGCGG GTCTGCGCAC CCGGGCCGAA CGGGACGGCG ACGAGTGGGT GATCAACGGG CAGAAGGTGT GGACGTCGTT CGCCCAGACC GCCGACTACG GACTGCTGGT CGCCCGCACC GACTGGGATG TGCCCAAACA CAAGGGCATC AGCTTCTTCA TGCTGCCGAT GAAGCAACCC GGCATCGAGG TGCGGCCGAT CCACCAGATC ACCGGGGAGT CGGAGTTCAA CGAGGTGTTC ATCACCGACG CCCGCTGCCC GGCGGAGAAC ATGGTCGGCC GGCCCGGCGA GGGCTGGTCG GTGCTGCAGG TCGCGCTCGG CTATGAGCGG CGGCTGATGG GTGATCTGGC CCGAACCTCG AAGGCCAGCC GCAAACCCAA GGACGACCCC GCCCAGCAGC GCAACGCGCT GGTCGAGATG GCCCGGGAGG CCGGCAGACT CGATGATCCC TACATCCGGC AGCGGATCGC CCGGATGGAC GAGTTCGCGG CGGTCAACCG GTGGAACACC CAGCGGTCGA AGGCCACCCG CGACAAGGTG GAGTCGTTGA CGTTGCAGGC GCTCGGCAAG ATCGCGATGT CGCGGATCCT GCACGAGACC GCGCGGGTGC AGACCGAGAT CGTCGGCGCC GAGTCGATGC TGGCCGGCCC GGACCATCCG ATCGGTGACG GTGTGACGTT CCGCACGCTC AACGCGTATT TCACGTCGAT CGGCGGTGGG ACCGACCAGG TGCAGCGCAA CATCGTCGGC GAACGGGTGC TGGGCCTGCC CAAGGAGCCG GATCCCTACC GGGATCAACC CTTCCGCGAC CTCCCCCAC
Details for MythA.01613.k.A1.GE28803
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 91
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISRWPRRI ARTPTSTSPP PTSRCTAASA SPGSIRRTCI CGGPAPTRNC SAIRPGTVSA TCSGLEPDMS ELTDDQVRTE VREWLAANWK PGMDRAEWAR LVFEAGWAVP SWEPQWYGRG LTDPQSRIVA AEFAAVGAPG TGHDRANLFA CTLHDLGTEE QKRRLIPPSI RGETRWCLLY SEPGAGSDLA GLRTRAERDG DEWVINGQKV WTSFAQTADY GLLVARTDWD VPKHKGISFF MLPMKQPGIE VRPIHQITGX SXFNEVFITD ARCPAENMVG RPXEXWSVLQ XXXGYERRLM GDLARTSKAS XXPKDDPAQQ RNALVEMARE AGRLDDPYIR QRIARMDEFA AVNRWNTQRS KATRDKVESL TLQALGKIAM SRILHETARV QTEIVGAESM LAGPDHPIGD GVTFRTLNAY FTSIGGGTDQ VQRNIVGERV LGLPKEPDPY RDQPFRXLPH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatct cgcgctggcc caggcgtatt gctcggacgc ctacgtctac gtcgccgcca ccaacatcca ggtgcacggc ggcatcggct tcacctggga gcatccggcg cacctgtatc tgcggcgggc ccgcaccgac gcgcaactgt tcggcgatcc ggcctggcac cgtgagcgct acctgcagcg gattggagcc tgacatgagc gagttgaccg acgatcaggt ccggaccgag gtccgcgagt ggctggccgc gaactggaaa cccgggatgg accgcgccga atgggcccgg ctggtgttcg aggccggctg ggcggttccc agctgggagc cgcagtggta cggccgcggc ctgaccgacc cccagtcccg catcgtggca gccgaattcg ctgcggtggg cgcccccggc accggccacg accgggccaa cctgttcgcc tgcaccctgc acgatctggg caccgaggag cagaagcggc ggctgatccc gccgtccatc cgcggtgaga cccggtggtg tctgctctac agcgaaccgg gcgccggatc ggacctcgcg ggtctgcgca cccgggccga acgggacggc gacgagtggg tgatcaacgg gcagaaggtg tggacgtcgt tcgcccagac cgccgactac ggactgctgg tcgcccgcac cgactgggat gtgcccaaac acaagggcat cagcttcttc atgctgccga tgaagcaacc cggcatcgag gtgcggccga tccaccagat caccggggan tcgganttca acgaggtgtt catcaccgac gcccgctgcc cggcggagaa catggtcggc cggcccgncg agggntggtc ggtgctgcag gtnnnnntng gctatgagcg gcggctgatg ggtgatctgg cccgaacctc gaaggccagc nnnnaaccca aggacgaccc cgcccagcag cgcaacgcgc tggtcgagat ggcccgggag gccggcagac tcgatgatcc ctacatccgg cagcggatcg cccggatgga cgagttcgcg gcggtcaacc ggtggaacac ccagcggtcg aaggccaccc gcgacaaggt ggagtcgttg acgttgcagg cgctcggcaa gatcgcgatg tcgcggatcc tgcacgagac cgcgcgggtg cagaccgaga tcgtcggcgc cgagtcgatg ctggccggcc cggaccatcc gatcggtgac ggtgtgacgt tccgcacgct caacgcgtat ttcacgtcga tcggcggtgg gaccgaccag gtgcagcgca acatcgtcgg cgaacgggtg ctgggcctgc ccaaggagcc ggatccctac cgggatcaac ccttccgcgn cctcccccac taataancag cangannnag tt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISRWPRRI ARTPTSTSPP PTSRCTAASA SPGSIRRTCI CGGPAPTRNC SAIRPGTVSA TCSGLEPDMS ELTDDQVRTE VREWLAANWK PGMDRAEWAR LVFEAGWAVP XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXPGTGHDR ANLFACTLHD LGTEEQKRRL IPPSIRGETR WCLLYSEPGA GSDLAGLRTR AERDGDEWVI NGQKVWTSFA QTADYGLLVA RTDWDVPKHK GISFFMLPMK QPGIEVRPIH QITGESEFNE VFITDARCPA ENMVGRPGEG WSVLQVALGY ERRLMGDLAR TSKASRKPKD DPAQQRNALV EMAREAGRLD DPYIRQRIAR MDEFAAVNRW NTQRSKATRD KVESLTLQAL GKIAMSRILH ETARVQTEIV GAESMLAGPD HPIGDGVTFR TLNAYFTSIG GGTDQVQRNI VGERVLGLPK EPDPYRDQPF RDLPH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctcgcgc tggcccaggc gtattgctcg gacgcctacg tctacgtcgc cgccaccaac atccaggtgc acggcggcat cggcttcacc tgggagcatc cggcgcacct gtatctgcgg cgggcccgca ccgacgcgca actgttcggc gatccggcct ggcaccgtga gcgctacctg cagcggattg gagcctgaca tgagcgagtt gaccgacgat caggtccgga ccgaggtccg cgagtggctg gccgcgaact ggaaacccgg gatggaccgc gccgaatggg cccggctggt gttcgaggcc ggctgggcgg ttcccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccggc accggccacg accgggccaa cctgttcgcc tgcaccctgc acgatctggg caccgaggag cagaagcggc ggctgatccc gccgtccatc cgcggtgaga cccggtggtg tctgctctac agcgaaccgg gcgccggatc ggacctcgcg ggtctgcgca cccgggccga acgggacggc gacgagtggg tgatcaacgg gcagaaggtg tggacgtcgt tcgcccagac cgccgactac ggactgctgg tcgcccgcac cgactgggat gtgcccaaac acaagggcat cagcttcttc atgctgccga tgaagcaacc cggcatcgag gtgcggccga tccaccagat caccggggag tcggagttca acgaggtgtt catcaccgac gcccgctgcc cggcggagaa catggtcggc cggcccggcg agggctggtc ggtgctgcag gtcgcgctcg gctatgagcg gcggctgatg ggtgatctgg cccgaacctc gaaggccagc cgcaaaccca aggacgaccc cgcccagcag cgcaacgcgc tggtcgagat ggcccgggag gccggcagac tcgatgatcc ctacatccgg cagcggatcg cccggatgga cgagttcgcg gcggtcaacc ggtggaacac ccagcggtcg aaggccaccc gcgacaaggt ggagtcgttg acgttgcagg cgctcggcaa gatcgcgatg tcgcggatcc tgcacgagac cgcgcgggtg cagaccgaga tcgtcggcgc cgagtcgatg ctggccggcc cggaccatcc gatcggtgac ggtgtgacgt tccgcacgct caacgcgtat ttcacgtcga tcggcggtgg gaccgaccag gtgcagcgca acatcgtcgg cgaacgggtg ctgggcctgc ccaaggagcc ggatccctac cgggatcaac ccttccgcga cctcccccac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa