MythA.01613.j

Acyl-CoA dehydrogenase domain protein (A0R6D6 homolog)

CENTER ID: MythA.01613.j
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01613.j.A1.GE28802 Full length( MythA.01613.j ) 1 411
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3847
RefSeq: WP_003927371.1
UniProt: G7CLL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRSVGRLT NEVCVVKLAL SDADAAFRAE MREFFTTEIP ADIRDRMRAG RPRHXXXTTS SPPSASSTRT VWRCRTGRWS GXXXXXXXXX XXXLQRQIWS DEMQLAAVPE PLAFNANMVG PVIARFGSQQ LKERFLPPTA NLDIWWCQGF SEPEAGSDLA SLRTTAVRDG DSYVVNGQKT WTTLGQYADW IFLLARTDPD APKRQAGISF LLADMNTPGI TLRPIKLIDG GHEVNEVFFE DVRIPADQLV GEENHGWAYA KFLLSNERTG IARIGATKLW LSQVKSRASR AAECSLLDDP LFAARIAELE NDLLALELTQ LRVSGSEGEG KPNPASSILK LRGSQLQQAV TELAVEVAGT DALPYAAGDD IASPEWAQLA APRYLNYRKT SIYGGTNEVQ RTIIASTILG L
NT Sequence
CTGCTCCGAC GATCCGTTGG ACGACTAACG AATGAGGTCT GTGTCGTGAA GCTGGCGCTC AGTGATGCCG ATGCCGCGTT CCGGGCGGAG ATGCGGGAGT TCTTCACCAC CGAGATTCCC GCCGACATCC GGGACCGGAT GCGAGCCGGC CGGCCGCGCC ACCNNNNNNC GACGACATCG TCACCACCCA GCGCATCCTC AACGCGCACG GTCTGGCGGT GCCGAACTGG CCGGTGGAGT GGGGNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TGCAGCGCCA GATCTGGTCC GACGAGATGC AACTGGCCGC GGTGCCCGAA CCGCTGGCGT TCAACGCCAA CATGGTCGGA CCGGTGATCG CCCGGTTCGG GTCCCAACAG CTCAAGGAGC GGTTCCTGCC GCCGACCGCC AACCTCGACA TCTGGTGGTG CCAGGGCTTC TCCGAACCGG AGGCGGGCTC CGACCTCGCG TCGTTGCGCA CCACCGCGGT GCGGGACGGC GACAGCTACG TCGTCAACGG CCAGAAGACC TGGACGACGC TGGGTCAGTA CGCCGACTGG ATCTTCCTGC TGGCGCGCAC CGACCCGGAC GCTCCGAAAA GGCAGGCCGG CATCTCATTC CTGTTGGCCG ACATGAACAC CCCCGGCATC ACGCTGCGGC CGATCAAGCT GATCGACGGC GGCCACGAGG TGAACGAGGT GTTCTTCGAA GACGTCCGGA TCCCGGCCGA TCAACTCGTG GGCGAGGAGA ACCACGGCTG GGCCTACGCC AAGTTCCTGC TCAGCAACGA ACGCACCGGC ATCGCCCGGA TCGGGGCCAC CAAACTGTGG CTGTCCCAGG TCAAATCCCG CGCNTCCCGC GCTGCCGAAT GTAGTCTGCT CGACGATCCG CTGTTCGCGG CCCGCATCGC CGAACTGGAG AACGACCTGC TGGCGCTGGA GCTGACCCAG CTGCGGGTCA GCGGATCCGA GGGTGAGGGC AAACCCAACC CGGCCTCGTC GATCCTGAAA CTACGCGGCA GCCAGCTGCA GCAGGCGGTC ACCGAGCTGG CGGTCGAGGT CGCCGGGACC GATGCGCTGC CGTACGCCGC CGGCGACGAC ATCGCCTCGC CGGAGTGGGC GCAACTCGCC GCACCGCGTT ACCTCAACTA CCGCAAGACC TCCATCTACG GGGGCACCAA CGAGGTGCAG CGCACCATCA TCGCCTCGAC CATTCTCGGA TTG
Details for MythA.01613.j.A1.GE28802
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 92
PERCENT COVERAGE: 92
Validated AA Sequence
MLLRRSVGRL TNEVCVVKLA LSDADAAFRA EMREFFTTEI PADIRDRMRA GRPRYPDDIV TTQRILNAHG LAVPNWPVEW GGRDWSPLQR QIWSDEMQLA AVPEPLAFNA NMVGPVIARF GSQQLKERFL PPTANLDIWW CQGFSEPEAG SDLASLRTTA VRDGDSYVVN GQKTWTTLGQ YADWIFLLAR TDPDAPKRQA GISFLLADMN TPGITLRPIK LIDGGHEVNE VFFEDVRIPA DQLVGEENHG WAYAKFLLSN ERTGIARIGA TKLWLSQVKS RAAECSLLDD PLFAARIAEL ENDLLALELT QLRVSGSEGE GKPNPASSIL KLRGSQLQQA VTELAVEVAG TDALPYAAGD DIASPEWAQL AAPRYLNYRK TSIYGGTNEV QRTIIASTIL GL
Validated NT Sequence
ttattacaat ccgagaatgg tcgaggcgat gatggtgcgc tgcacctcgt tggtgccccc gtagatggag gtcttgcggt agttgaggta acgcggtgcg gcgagttgcg cccactccgg cgaggcgatg tcgtcgccgg cggcgtacgg cagcgcatcg gtcccggcga cctcgaccgc cagctcggtg accgcctgct gcagctggct gccgcgtagt ttcaggatcg acgaggccgg gttgggtttg ccctcaccct cggatccgct gacccgcagc tgggtcagct ccagcgccag caggtcgttc tccagttcgg cgatgcgggc cgcgaacagc ggatcgtcga gcagactaca ttcggcagcg cgggatttga cctgggacag ccacagtttg gtggccccga tccgggcgat gccggtgcgt tcgttgctga gcaggaactt ggcgtaggcc cagccgtggt tctcctcgcc cacgagttga tcggccggga tccggacgtc ttcgaagaac acctcgttca cctcgtggcc gccgtcgatc agcttgatcg gccgcagcgt gatgccgggg gtgttcatgt cggccaacag gaatgagatg ccggcctgcc ttttcggagc gtccgggtcg gtgcgcgcca gcaggaagat ccagtcggcg tactgaccca gcgtcgtcca ggtcttctgg ccgttgacga cgtagctgtc gccgtcccgc accgcggtgg tgcgcaacga cgcgaggtcg gagcccgcct ccggttcgga gaagccctgg caccaccaga tgtcgaggtt ggcggtcggc ggcaggaacc gctccttgag ctgttgggac ccgaaccggg cgatcaccgg tccgaccatg ttggcgttga acgccagcgg ttcgggcacc gcggccagtt gcatctcgtc ggaccagatc tggcgctgca gtggcgacca gtcccggccg ccccactcca ccggccagtt cggcaccgcc agaccgtgcg cgttgaggat gcgctgggtg gtgacgatgt cgtcggggta gcgcggccgg ccggctcgca tccggtcccg gatgtcggcg ggaatctcgg tggtgaagaa ctcccgcatc tccgcccgga acgcggcatc ggcatcactg agcgccagct tcacgacaca gacctcattc gttagtcgtc caacggatcg tcggagcagc atcgaaccag gaccctgggt ctgagcttcc agggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLRRSVGR LTNEVCVVKL ALSDADAAFR AEMREFFTTE IPADIRDRMR AGRPRHXXXT TSSPPSASST RTVWRCRTGR WSGXXXXXXX XXXXXLQRQI WSDEMQLAAV PEPLAFNANM VGPVIARFGS QQLKERFLPP TANLDIWWCQ GFSEPEAGSD LASLRTTAVR DGDSYVVNGQ KTWTTLGQYA DWIFLLARTD PDAPKRQAGI SFLLADMNTP GITLRPIKLI DGGHEVNEVF FEDVRIPADQ LVGEENHGWA YAKFLLSNER TGIARIGATK LWLSQVKSRX SRAAECSLLD DPLFAARIAE LENDLLALEL TQLRVSGSEG EGKPNPASSI LKLRGSQLQQ AVTELAVEVA GTDALPYAAG DDIASPEWAQ LAAPRYLNYR KTSIYGGTNE VQRTIIASTI LGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgctccga cgatccgttg gacgactaac gaatgaggtc tgtgtcgtga agctggcgct cagtgatgcc gatgccgcgt tccgggcgga gatgcgggag ttcttcacca ccgagattcc cgccgacatc cgggaccgga tgcgagccgg ccggccgcgc caccnnnnnn cgacgacatc gtcaccaccc agcgcatcct caacgcgcac ggtctggcgg tgccgaactg gccggtggag tggggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgcagcgcc agatctggtc cgacgagatg caactggccg cggtgcccga accgctggcg ttcaacgcca acatggtcgg accggtgatc gcccggttcg ggtcccaaca gctcaaggag cggttcctgc cgccgaccgc caacctcgac atctggtggt gccagggctt ctccgaaccg gaggcgggct ccgacctcgc gtcgttgcgc accaccgcgg tgcgggacgg cgacagctac gtcgtcaacg gccagaagac ctggacgacg ctgggtcagt acgccgactg gatcttcctg ctggcgcgca ccgacccgga cgctccgaaa aggcaggccg gcatctcatt cctgttggcc gacatgaaca cccccggcat cacgctgcgg ccgatcaagc tgatcgacgg cggccacgag gtgaacgagg tgttcttcga agacgtccgg atcccggccg atcaactcgt gggcgaggag aaccacggct gggcctacgc caagttcctg ctcagcaacg aacgcaccgg catcgcccgg atcggggcca ccaaactgtg gctgtcccag gtcaaatccc gcgcntcccg cgctgccgaa tgtagtctgc tcgacgatcc gctgttcgcg gcccgcatcg ccgaactgga gaacgacctg ctggcgctgg agctgaccca gctgcgggtc agcggatccg agggtgaggg caaacccaac ccggcctcgt cgatcctgaa actacgcggc agccagctgc agcaggcggt caccgagctg gcggtcgagg tcgccgggac cgatgcgctg ccgtacgccg ccggcgacga catcgcctcg ccggagtggg cgcaactcgc cgcaccgcgt tacctcaact accgcaagac ctccatctac gggggcacca acgaggtgca gcgcaccatc atcgcctcga ccattctcgg attgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa