SSGCID: Target Page

MythA.01613.i

Putative acyl-CoA dehydrogenase (A0R500 homolog)

CENTER ID:	MythA.01613.i
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01613.i.A1.GE28801	Full length( MythA.01613.i )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1510
RefSeq:	WP_003925033.1
UniProt:	G7CEW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLHIDDATE EFRAEVREFL ANNRKWFPTK SYDTAEGFEQ HRQWDRVLYD ARLSVITWPE KYGGRDATLL QWVVFEEEYF RAGAPGRASA NGTSMLAPTL FAHGTEEQLD RVLPKMATGE EIWAQAWSEP ESGSDLASLR STATKTDGGW LLNGQKIWSS RAVFGERAFG LFRSDPQAQR HKGLTYFMFD LRADGVTVRP IAQLGGDTGF GEIFLDNVFV PDNDVIGEVN EGWRAAMSTS SNERGMSLRS PARFLAPAER LVRLWKQNPD PAFEERVADA WIKAQAYRLH TFGTVSRLAR GGELGAESSV TKVFWSELDV ALHQTALDLR GPDAELADSA DRDEDGYSWT DGLLFALGGP IYAGTNEIQR NIIAERLLGL PRK

NT Sequence

ATGGATCTGC ACATCGACGA CGCCACCGAG GAGTTCCGGG CCGAGGTCCG CGAATTCCTG GCGAACAACC GGAAATGGTT CCCCACCAAG TCCTATGACA CCGCCGAGGG TTTCGAGCAG CACCGCCAGT GGGACCGGGT GCTCTATGAC GCGCGGCTGA GCGTCATCAC CTGGCCGGAG AAGTACGGCG GCCGCGACGC GACGCTGCTG CAGTGGGTGG TGTTCGAGGA GGAGTACTTC CGGGCCGGCG CGCCGGGACG GGCCAGCGCC AACGGCACCT CGATGCTGGC GCCCACCCTG TTCGCCCACG GCACCGAGGA GCAACTCGAC CGGGTGCTGC CGAAAATGGC CACCGGCGAG GAGATCTGGG CTCAGGCCTG GTCCGAGCCG GAGTCGGGCA GCGATCTGGC GTCACTGCGC TCGACGGCGA CCAAGACCGA CGGCGGCTGG CTGCTCAACG GACAGAAGAT CTGGAGTTCG CGGGCGGTGT TCGGCGAACG CGCCTTCGGC CTGTTCCGCT CCGACCCGCA GGCGCAGCGC CACAAGGGCC TGACCTACTT CATGTTCGAC CTCAGGGCCG ACGGGGTGAC GGTGCGGCCG ATCGCCCAAC TCGGTGGTGA CACCGGGTTC GGCGAGATCT TCCTCGACAA CGTGTTCGTG CCCGACAACG ATGTGATCGG CGAGGTCAAC GAGGGTTGGC GCGCCGCGAT GAGCACGTCG AGCAACGAAC GCGGCATGTC GCTGCGCAGC CCGGCGCGCT TCCTGGCGCC GGCCGAACGG CTGGTGCGGC TGTGGAAACA GAACCCCGAC CCGGCGTTCG AGGAGCGGGT TGCCGACGCC TGGATCAAGG CGCAGGCCTA CCGGCTGCAC ACCTTCGGAA CCGTGAGCCG GCTGGCCCGC GGCGGTGAGC TGGGCGCGGA ATCGTCGGTG ACCAAGGTGT TCTGGTCGGA GCTGGACGTC GCGCTGCACC AGACCGCGCT GGACCTGCGT GGCCCGGACG CCGAACTGGC CGACTCCGCC GACCGCGACG AGGACGGCTA CTCCTGGACC GACGGTCTGT TGTTCGCGCT CGGCGGCCCG ATCTACGCCG GCACCAACGA GATCCAGCGC AACATCATCG CCGAGCGGCT GCTCGGACTC CCCCGCAAA

Details for MythA.01613.i.A1.GE28801

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDLHIDDAT EEFRAEVREF LANNRKWFPT KSYDTAEGFE QHRQWDRVLY DARLSVITWP EKYGGRDATL LQWVVFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTEEQL DRVLPKMATG EEIWAQAWSE PESGSDLASL RSTATKTDGG WLLNGQKIWS SRAVFGERAF GLFRSDPQAQ RHKGLTYFMF DLRADGVTVR PIAQLGGDTG FGEIFLDNVF VPDNDVIGEV NEGWRAAMST SSNERGMSLR SPARFLAPAE RLVRLWKQNP DPAFEERVAD AWIKAQAYRL HTFGTVSRLA RGGELGAESS VTKVFWSELD VALHQTALDL RGPDAELADS ADRDEDGYSW TDGLLFALGG PIYAGTNEIQ RNIIAERLLG LPRK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatctgcac atcgacgacg ccaccgagga gttccgggcc gaggtccgcg aattcctggc gaacaaccgg aaatggttcc ccaccaagtc ctatgacacc gccgagggtt tcgagcagca ccgccagtgg gaccgggtgc tctatgacgc gcggctgagc gtcatcacct ggccggagaa gtacggcggc cgcgacgcga cgctgctgca gtgggtggtg ttcgaggagg agtacttccg ggccggcgcg ccgggacggg ccagcgccaa cggcacctcg atgctggcgc ccaccctgtt cgcccacggc accgaggagc aactcgaccg ggtgctgccg aaaatggcca ccggcgagga gatctgggct caggcctggt ccgagccgga gtcgggcagc gatctggcgt cactgcgctc gacggcgacc aagaccgacg gcggctggct gctcaacgga cagaagatct ggagttcgcg ggcggtgttc ggcgaacgcg ccttcggcct gttccgctcc gacccgcagg cgcagcgcca caagggcctg acctacttca tgttcgacct cagggccgac ggggtgacgg tgcggccgat cgcccaactc ggtggtgaca ccgggttcgg cgagatcttc ctcgacaacg tgttcgtgcc cgacaacgat gtgatcggcg aggtcaacga gggttggcgc gccgcgatga gcacgtcgag caacgaacgc ggcatgtcgc tgcgcagccc ggcgcgcttc ctggcgccgg ccgaacggct ggtgcggctg tggaaacaga accccgaccc ggcgttcgag gagcgggttg ccgacgcctg gatcaaggcg caggcctacc ggctgcacac cttcggaacc gtgagccggc tggcccgcgg cggtgagctg ggcgcggaat cgtcggtgac caaggtgttc tggtcggagc tggacgtcgc gctgcaccag accgcgctgg acctgcgtgg cccggacgcc gaactggccg actccgccga ccgcgacgag gacggctact cctggaccga cggtctgttg ttcgcgctcg gcggcccgat ctacgccggc accaacgaga tccagcgcaa catcatcgcc gagcggctgc tcggactccc ccgcaaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa