MythA.01613.h

Putative acyl-CoA dehydrogenase (A0R4Z9 homolog)

CENTER ID: MythA.01613.h
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01613.h.A1.GE28800 Full length( MythA.01613.h ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_003926715.1
UniProt: G7CHZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTTSDVAED IEELRAQFRE FLANAPKPQG LRNYGPTPTA DDIAPGRAWH KFLAANGYVC LHWPVRHGGA DASVAFQATF AEECARAAVP RQLAITAIDL VGPVLIEFGT EEQKARYLEP IRLGDDIWTQ LFSEPDAGSD LAGIRTKAEK TISGWRINGQ KVWSSGANSA KFGLLLARTG PESHGGLSMF VVPMDAEGVS VRPLQQMDGE SKFNEVFLDN VDLPADALIG EPGQGWRIAM VTLGRERLTL GSHAVVMFEL HHRLVQAARA RDALDPTLMR AMIRLWIRIW LLRYTWQRAI DESDLQSSAF SVLKLMTSET DRDLGDLATD VLGTDACVDP SGGSEEAELV RAMLVGRAQT ILGGTSEIQR NILGERVLGL PKEPR
NT Sequence
GTGGTAACCA CCTCCGACGT AGCCGAGGAT ATCGAGGAAC TGCGAGCGCA GTTCCGTGAG TTCCTCGCGA ACGCCCCCAA GCCTCAGGGT CTCCGCAACT ACGGGCCCAC GCCGACGGCC GACGACATCG CCCCTGGGCG GGCCTGGCAC AAGTTCCTCG CGGCCAACGG CTACGTGTGC CTACACTGGC CCGTCCGACA CGGCGGAGCC GATGCTTCGG TAGCATTCCA GGCCACTTTT GCCGAGGAAT GTGCCAGGGC AGCGGTGCCG CGGCAATTGG CAATCACAGC TATCGACCTA GTCGGCCCTG TTTTGATCGA GTTCGGCACC GAAGAACAGA AAGCCCGCTA TCTCGAGCCT ATCCGGCTAG GTGACGATAT CTGGACCCAA CTGTTCTCCG AACCGGATGC GGGTTCGGAT CTGGCGGGGA TACGCACCAA GGCAGAGAAG ACCATCAGCG GTTGGCGCAT CAACGGACAG AAGGTTTGGA GTTCGGGCGC CAACTCCGCG AAGTTCGGCC TGCTGCTTGC CCGCACCGGC CCGGAGTCGC ACGGCGGCCT CAGCATGTTC GTCGTACCGA TGGATGCCGA GGGCGTTTCG GTGCGTCCAC TGCAGCAGAT GGACGGCGAG AGCAAGTTCA ACGAAGTTTT CCTCGACAAC GTCGACCTAC CTGCCGATGC GCTGATCGGA GAGCCTGGAC AAGGCTGGAG GATCGCCATG GTGACTTTGG GCCGTGAGCG GTTGACGCTA GGCTCGCACG CCGTCGTGAT GTTCGAACTG CACCATCGCC TGGTCCAAGC GGCGCGCGCC CGCGACGCGC TGGACCCAAC GCTAATGCGG GCCATGATCA GACTTTGGAT ACGGATCTGG CTGCTGCGTT ACACGTGGCA GCGCGCTATC GACGAAAGTG ACCTGCAGTC CTCGGCATTT TCGGTGCTGA AGTTAATGAC ATCGGAGACC GACCGCGACC TCGGCGACCT GGCTACCGAC GTGCTCGGCA CCGACGCCTG CGTGGACCCC AGCGGCGGTT CGGAGGAAGC CGAACTCGTC CGCGCTATGT TAGTGGGCCG CGCGCAAACG ATCCTCGGCG GCACCAGCGA AATTCAGCGC AACATCCTTG GCGAGCGGGT ATTAGGTCTA CCTAAAGAGC CACGG
Details for MythA.01613.h.A1.GE28800
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 94
Validated AA Sequence
MGTLEAQTQG PGSMVVTTSD VAEDIEELRA QFREFLANAP KPQGLRNYGP TPTADDIAPG RAWHKFLAAN GYVCLHWPVR HGGADASVAF QATFAEECAR AAVPRQLAIT AIDLVGPVLI EFGTEEQKAR YLEPIRLGDD IWTQLFSEPD AGSDLAGIRT KAEKTISGWR INGQKVWSSG ANSAKFGLLL ARTGPESHGG LSMFVVPMDA EGVSVRPLQQ MDGESKFNEV FLDNVDLPAD ALIGEPGQGW RXAMVTLGRE RLTLGSHAVV MFELHHRLVQ AARARDALDP TLMRAMIRLW IRIWLLRYTW QRAIDESDLQ SSAFSVXKLM TXETDRXLXD LATDVLGTDA CVDPSGGSEE AELVRAMLVG RAQTILGGTS EIQRNI
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggta accacctccg acgtagccga ggatatcgag gaactgcgag cgcagttccg tgagttcctc gcgaacgccc ccaagcctca gggtctccgc aactacgggc ccacgccgac ggccgacgac atcgcccctg ggcgggcctg gcacaagttc ctcgcggcca acggctacgt gtgcctacac tggcccgtcc gacacggcgg agccgatgct tcggtagcat tccaggccac ttttgccgag gaatgtgcca gggcagcggt gccgcggcaa ttggcaatca cagctatcga cctagtcggc cctgttttga tcgagttcgg caccgaagaa cagaaagccc gctatctcga gcctatccgg ctaggtgacg atatctggac ccaactgttc tccgaaccgg atgcgggttc ggatctggcg gggatacgca ccaaggcaga gaagaccatc agcggttggc gcatcaacgg acagaaggtt tggagttcgg gcgccaactc cgcgaagttc ggcctgctgc ttgcccgcac cggcccggag tcgcacggcg gcctcagcat gttcgtcgta ccgatggatg ccgagggcgt ttcggtgcgt ccactgcagc agatggacgg cgagagcaag ttcaacgaag ttttcctcga caacgtcgac ctacctgccg atgcgctgat cggagagcct ggacaaggct ggaggnncgc catggtgact ttgggccgtg agcggttgac gctaggctcg cacgccgtcg tgatgttcga actgcaccat cgcctggtcc aagcggcgcg cgcccgcgac gcgctggacc caacgctaat gcgggccatg atcagacttt ggatacggat ctggctgctg cgttacacgt ggcagcgcgc tatcgacgaa agtgacctgc agtcctcggc attttcggtg ntgaagttaa tgacatngga gaccgaccgn nncctngncg acctggctac cgacgtgctn ggcaccgacg cntgcgtgga ccccagcggc ggttcggagg aagccgaact cgtccgcgct atgttagtgg gccgcgcgca aacgatcctn ggcggcacca gcgaaattca gcgcaacatc ctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVTTSDVA EDIEELRAQF REFLANAPKP QGLRNYGPTP TADDIAPGRA WHKFLAANGY VCLHWPVRHG GADASVAFQA TFAEECARAA VPRQLAITAI DLVGPVLIEF GTEEQKARYL EPIRLGDDIW TQLFSEPDAG SDLAGIRTKA EKTISGWRIN GQKVWSSGAN SAKFGLLLAR TGPESHGGLS MFVVPMDAEG VSVRPLQQMD GESKFNEVFL DNVDLPADAL IGEPGQGWRI AMVTLGRERL TLGSHAVVMF ELHHRLVQAA RARDALDPTL MRAMIRLWIR IWLLRYTWQR AIDESDLQSS AFSVLKLMTS ETDRDLGDLA TDVLGTDACV DPSGGSEEAE LVRAMLVGRA QTILGGTSEI QRNILGERVL GLPKEPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggtaacc acctccgacg tagccgagga tatcgaggaa ctgcgagcgc agttccgtga gttcctcgcg aacgccccca agcctcaggg tctccgcaac tacgggccca cgccgacggc cgacgacatc gcccctgggc gggcctggca caagttcctc gcggccaacg gctacgtgtg cctacactgg cccgtccgac acggcggagc cgatgcttcg gtagcattcc aggccacttt tgccgaggaa tgtgccaggg cagcggtgcc gcggcaattg gcaatcacag ctatcgacct agtcggccct gttttgatcg agttcggcac cgaagaacag aaagcccgct atctcgagcc tatccggcta ggtgacgata tctggaccca actgttctcc gaaccggatg cgggttcgga tctggcgggg atacgcacca aggcagagaa gaccatcagc ggttggcgca tcaacggaca gaaggtttgg agttcgggcg ccaactccgc gaagttcggc ctgctgcttg cccgcaccgg cccggagtcg cacggcggcc tcagcatgtt cgtcgtaccg atggatgccg agggcgtttc ggtgcgtcca ctgcagcaga tggacggcga gagcaagttc aacgaagttt tcctcgacaa cgtcgaccta cctgccgatg cgctgatcgg agagcctgga caaggctgga ggatcgccat ggtgactttg ggccgtgagc ggttgacgct aggctcgcac gccgtcgtga tgttcgaact gcaccatcgc ctggtccaag cggcgcgcgc ccgcgacgcg ctggacccaa cgctaatgcg ggccatgatc agactttgga tacggatctg gctgctgcgt tacacgtggc agcgcgctat cgacgaaagt gacctgcagt cctcggcatt ttcggtgctg aagttaatga catcggagac cgaccgcgac ctcggcgacc tggctaccga cgtgctcggc accgacgcct gcgtggaccc cagcggcggt tcggaggaag ccgaactcgt ccgcgctatg ttagtgggcc gcgcgcaaac gatcctcggc ggcaccagcg aaattcagcg caacatcctt ggcgagcggg tattaggtct acctaaagag ccacggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa