MythA.01613.g

Putative acyl-CoA dehydrogenase (A0R4Z9 homolog)

CENTER ID: MythA.01613.g
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01613.g.A1.GE28799 Full length( MythA.01613.g ) 1 709
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.2876
RefSeq: WP_003926409.1
UniProt: G7CH35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDHGRRPAG LRDITQHLVR DTAEGRQQTV GVSDRRQQRR GIEHPARLLE HGGQADDTSD APELLRHTEQ VHPQGLDGVP PGLPGRLRPG VDLIHRGSGD EEAPGHLGEF LLLFIECKAH HHILGQNILR SIMLSMDLNL SESERTFRGR VRAWLADAPR PDGLRDYGAT PTLEDLPAAR EWQRTLLDAG FAGLSWPRSH GGTEATPVEQ GIWAEETARA GVPLQINLFG PELAGPMIIK FGTEAQQQRF LPKILRAEDI WCQLFSEPEA GSDLARLRTR AEPDGDGWRV HGQKVWTSGA HYADLGLLLX XXXXXXXGDR PHDGLTFFLL PMDRPGIEVR PLRQMDGQYK FNEVYFDGVY VGPEDVLGEV GSGWRVAMST LGRERMMIGA NAVRFTQALH DLWSDLDARG LTAGDAVRRR WTDLWSRSQL LRLQWFRVLS LVRSTDDSRT SLLKLTATEL ERDITVFAQE TLGPDLVTGS XXXXARWRDW LLAAPGQTIA GGTSEIQRNI IARRVLGXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXS CGAQLSTAVS STRSTMATIG IPARSAGRPG RCPRSRTSGY SWTLTSXXXX XXXXXXXXXX XXXXXXXXXX DGTAAGDLPP VGPPRPAEPA VRPRQVQDLI TPAAEAQRQG TFARGGEERT GLVVVGVGHR TRHHGLLRII ALVDTGIPDV TRHTSAPST
NT Sequence
ATCCTCGACC ACGGCCGCCG CCCGGCCGGA CTCCGCGACA TCACGCAACA CCTTGTGCGC GACACCGCCG AGGGCCGCCA GCAAACCGTT GGCGTATCCG ACCGACGTCA ACAGCGGCGC GGGATAGAGC ACCCGGCCCG CCTGCTCGAG CACGGCGGCC AGGCCGACGA CACCTCCGAC GCCCCCGAAC TCCTCCGGCA CACCGAGCAG GTGCACCCCC AGGGCCTCGA CGGCGTGCCC CCAGGCCTTC CGGGCCGCCT CAGGCCCGGC GTCGACTTGA TCCATCGGGG CTCCGGAGAC GAAGAAGCTC CGGGCCACCT CGGCGAGTTC CTGCTGCTGT TCATCGAATG TAAAGCGCAT CACCACATCC TAGGCCAAAA CATACTACGC AGTATCATGC TATCCATGGA CCTGAACCTC TCCGAATCTG AGCGGACCTT CCGTGGGCGC GTCCGGGCCT GGCTCGCCGA CGCGCCGAGA CCCGATGGCC TTCGCGACTA CGGCGCGACT CCGACACTGG AGGACCTGCC GGCCGCCCGG GAATGGCAAC GCACGCTGCT CGACGCCGGG TTCGCCGGGT TGTCCTGGCC GCGGTCGCAC GGCGGCACCG AAGCCACCCC GGTCGAACAG GGGATCTGGG CCGAGGAGAC CGCGCGGGCC GGGGTTCCGT TGCAGATCAA TCTCTTCGGC CCCGAACTGG CCGGGCCGAT GATCATCAAG TTCGGCACCG AGGCGCAGCA GCAGCGATTC CTGCCCAAGA TCCTGCGGGC CGAGGACATC TGGTGCCAGC TGTTCTCCGA ACCGGAGGCC GGTTCGGACC TCGCCCGGCT GCGCACCCGC GCCGAACCGG ATGGTGACGG CTGGCGTGTC CACGGCCAGA AGGTGTGGAC CTCCGGGGCG CACTACGCCG ATCTCGGGTT GCTGCTNNNN NNNNNNNNNN NNNNNNNNNN CGGCGACCGC CCACACGACG GGTTGACGTT CTTCCTGCTC CCGATGGACC GGCCCGGGAT CGAGGTGCGG CCACTGCGGC AGATGGACGG CCAGTACAAG TTCAACGAGG TGTACTTCGA CGGGGTGTAC GTCGGGCCGG AGGATGTGCT CGGGGAGGTC GGCTCCGGCT GGCGGGTGGC GATGTCGACA CTGGGCCGTG AACGGATGAT GATCGGTGCC AACGCCGTAC GTTTCACCCA GGCGCTGCAC GACTTGTGGT CTGATCTCGA CGCCCGGGGG CTCACGGCCG GCGACGCCGT CCGGCGCCGG TGGACCGATC TGTGGTCGCG CAGTCAACTG TTGCGCCTGC AGTGGTTCCG GGTGTTGTCG CTGGTGAGGT CCACCGATGA CTCCCGCACG TCACTGCTCA AACTCACCGC CACCGAGCTC GAACGGGACA TCACCGTCTT CGCGCAGGAG ACGCTCGGGC CCGATCTCGT GACCGGCAGC GNNNNNNNNN GCGCACGGTG GCGGGACTGG CTGCTCGCCG CGCCCGGCCA GACCATCGCC GGTGGCACGT CGGAGATCCA GCGCAACATC ATCGCCCGGC GCGTCCTCGG ACNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNACTCC TGCGGCGCCC AGTTGAGCAC GGCGGTGTCG TCGACCCGTT CCACGATGGC GACCATCGGC ATTCCCGCCC GTAGCGCCGG CCGGCCGGGC AGGTGCCCGA GGAGCCGGAC CTCAGGGTAC TCGTGGACGC TGACCAGCAN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA GACGGAACCG CGGCCGGAGA CCTCCCGCCA GTCGGTCCGC CACGACCCGC AGAACCGGCA GTGCGCCCGC GGCAGGTGCA GGACCTGATC ACACCGGCTG CAGAAGCACA GCGCCAGGGC ACCTTCGCGC GCGGCGGCGA AGAACGGACC GGTCTCGTGG TCGTCGGTGT AGGGCACCGG ACGCGTCATC ACGGCCTCCT CAGGATCATC GCGCTGGTCG ACACCGGGAT TCCGGATGTC ACCAGACACA CTTCGGCCCC CTCGACC
Details for MythA.01613.g.A1.GE28799
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 80kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILDHGRRP AGLRDITQHL VRDTAEGRQQ TVGVSDRRQQ RRGIEHPARL LEHGGQADDT SDAPELLRHT EQVHPQGLDG VPPGLPGRLR PGVDLIHRGS GDEEAPGHLG EFLLLFIECK AHHHILGQNI LRSIMLSMDL NLSESERTFR GRVRAWLADA PRPDGLRDYG ATPTLEDLPA AREWQRTLLD AGFAGLSWPR SHGGTEATPV EQGXWAEETA RAGVPLQINL FGPELAGPMI IKFGTXAQQQ RXLPKILRAE XIWCQLFSEP XAGSDLARLX TRAEPXGXGW RVHGXK
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgatcctc gaccacggcc gccgcccggc cggactccgc gacatcacgc aacaccttgt gcgcgacacc gccgagggcc gccagcaaac cgttggcgta tccgaccgac gtcaacagcg gcgcgggata gagcacccgg cccgcctgct cgagcacggc ggccaggccg acgacacctc cgacgccccc gaactcctcc ggcacaccga gcaggtgcac ccccagggcc tcgacggcgt gcccccaggc cttccgggcc gcctcaggcc cggcgtcgac ttgatccatc ggggctccgg agacgaagaa gctccgggcc acctcggcga gttcctgctg ctgttcatcg aatgtaaagc gcatcaccac atcctaggcc aaaacatact acgcagtatc atgctatcca tggacctgaa cctctccgaa tctgagcgga ccttccgtgg gcgcgtccgg gcctggctcg ccgacgcgcc gagacccgat ggccttcgcg actacggcgc gactccgaca ctggaggacc tgccggccgc ccgggaatgg caacgcacgc tgctcgacgc cgggttcgcc gggttgtcct ggccgcggtc gcacggcggc accgaagcca ccccggtcga acagggganc tgggccgagg agaccgcgcg ggccggggtt ccgttgcaga tcaatctctt cggccccgaa ctggccgggc cgatgatcat caagttcggc accgangcgc agcagcagcg antcctgccc aagatcctgc gggccgagga natctggtgc cagctgttct ccgaaccgga ngccggttcg gacctcgccc ggctgcncac ccgcgccgaa ccggnnggtg anggctggcg tgtccacggn nagaagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILDHGRRP AGLRDITQHL VRDTAEGRQQ TVGVSDRRQQ RRGIEHPARL LEHGGQADDT SDAPELLRHT EQVHPQGLDG VPPGLPGRLR PGVDLIHRGS GDEEAPGHLG EFLLLFIECK AHHHILGQNI LRSIMLSMDL NLSESERTFR GRVRAWLADA PRPDGLRDYG ATPTLEDLPA AREWQRTLLD AGFAGLSWPR SHGGTEATPV EQGIWAEETA RAGVPLQINL FGPELAGPMI IKFGTEAQQQ RFLPKILRAE DIWCQLFSEP EAGSDLARLR TRAEPDGDGW RVHGQKVWTS GAHYADLGLL XXXXXXXXXG DRPHDGLTFF LLPMDRPGIE VRPLRQMDGQ YKFNEVYFDG VYVGPEDVLG EVGSGWRVAM STLGRERMMI GANAVRFTQA LHDLWSDLDA RGLTAGDAVR RRWTDLWSRS QLLRLQWFRV LSLVRSTDDS RTSLLKLTAT ELERDITVFA QETLGPDLVT GSXXXXARWR DWLLAAPGQT IAGGTSEIQR NIIARRVLGX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XSCGAQLSTA VSSTRSTMAT IGIPARSAGR PGRCPRSRTS GYSWTLTSXX XXXXXXXXXX XXXXXXXXXX XXDGTAAGDL PPVGPPRPAE PAVRPRQVQD LITPAAEAQR QGTFARGGEE RTGLVVVGVG HRTRHHGLLR IIALVDTGIP DVTRHTSAPS T
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcctcgac cacggccgcc gcccggccgg actccgcgac atcacgcaac accttgtgcg cgacaccgcc gagggccgcc agcaaaccgt tggcgtatcc gaccgacgtc aacagcggcg cgggatagag cacccggccc gcctgctcga gcacggcggc caggccgacg acacctccga cgcccccgaa ctcctccggc acaccgagca ggtgcacccc cagggcctcg acggcgtgcc cccaggcctt ccgggccgcc tcaggcccgg cgtcgacttg atccatcggg gctccggaga cgaagaagct ccgggccacc tcggcgagtt cctgctgctg ttcatcgaat gtaaagcgca tcaccacatc ctaggccaaa acatactacg cagtatcatg ctatccatgg acctgaacct ctccgaatct gagcggacct tccgtgggcg cgtccgggcc tggctcgccg acgcgccgag acccgatggc cttcgcgact acggcgcgac tccgacactg gaggacctgc cggccgcccg ggaatggcaa cgcacgctgc tcgacgccgg gttcgccggg ttgtcctggc cgcggtcgca cggcggcacc gaagccaccc cggtcgaaca ggggatctgg gccgaggaga ccgcgcgggc cggggttccg ttgcagatca atctcttcgg ccccgaactg gccgggccga tgatcatcaa gttcggcacc gaggcgcagc agcagcgatt cctgcccaag atcctgcggg ccgaggacat ctggtgccag ctgttctccg aaccggaggc cggttcggac ctcgcccggc tgcgcacccg cgccgaaccg gatggtgacg gctggcgtgt ccacggccag aaggtgtgga cctccggggc gcactacgcc gatctcgggt tgctgctnnn nnnnnnnnnn nnnnnnnnnn ncggcgaccg cccacacgac gggttgacgt tcttcctgct cccgatggac cggcccggga tcgaggtgcg gccactgcgg cagatggacg gccagtacaa gttcaacgag gtgtacttcg acggggtgta cgtcgggccg gaggatgtgc tcggggaggt cggctccggc tggcgggtgg cgatgtcgac actgggccgt gaacggatga tgatcggtgc caacgccgta cgtttcaccc aggcgctgca cgacttgtgg tctgatctcg acgcccgggg gctcacggcc ggcgacgccg tccggcgccg gtggaccgat ctgtggtcgc gcagtcaact gttgcgcctg cagtggttcc gggtgttgtc gctggtgagg tccaccgatg actcccgcac gtcactgctc aaactcaccg ccaccgagct cgaacgggac atcaccgtct tcgcgcagga gacgctcggg cccgatctcg tgaccggcag cgnnnnnnnn ngcgcacggt ggcgggactg gctgctcgcc gcgcccggcc agaccatcgc cggtggcacg tcggagatcc agcgcaacat catcgcccgg cgcgtcctcg gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnactc ctgcggcgcc cagttgagca cggcggtgtc gtcgacccgt tccacgatgg cgaccatcgg cattcccgcc cgtagcgccg gccggccggg caggtgcccg aggagccgga cctcagggta ctcgtggacg ctgaccagca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agacggaacc gcggccggag acctcccgcc agtcggtccg ccacgacccg cagaaccggc agtgcgcccg cggcaggtgc aggacctgat cacaccggct gcagaagcac agcgccaggg caccttcgcg cgcggcggcg aagaacggac cggtctcgtg gtcgtcggtg tagggcaccg gacgcgtcat cacggcctcc tcaggatcat cgcgctggtc gacaccggga ttccggatgt caccagacac acttcggccc cctcgaccaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa