SSGCID: Target Page

MythA.01613.b

Acyl-CoA dehydrogenase (A0R1R2 homolog)

CENTER ID:	MythA.01613.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01613.b.A1.GE28794	Full length( MythA.01613.b )		1	349

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2007
RefSeq:	WP_003925532.1
UniProt:	G7CGB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKANCSNDPP KSPHQHRPQA TISTPERRSP ETPRSTGIDN SSLPIPAQAW TLEVMGPATL KFAPRLATDF LPRYLDGSEW WGQGFSEPES GSDLASLRTR ATDDGAGGFI VNGQKIWTSQ GATATRLYVL VRTGTPESRH RGLSMLLIDS DAPGVTFRPI ALASGRRELA ELFFDDVRVP PERLVGEVGG GWAVTMFLMQ YERGMYGYAA LNKVMMELGR LRDYMVNRAV SAAQRERFAR VYVDVTSAQA RAAATVRKLA AGQVVGPESS IDKLLCSEAE REAHDLILDV TRDHMMVGTG TGIDPAAFDT ARAGWWYSRA ATIMGGTAEV QRGIIADHIL GLPKERRAS

NT Sequence

ATAAAGGCAA ACTGCTCGAA CGACCCACCG AAATCACCCC ACCAACACCG CCCTCAGGCG ACGATCAGCA CACCGGAACG GAGGTCGCCT GAAACACCCC GATCCACAGG TATTGACAAT TCCTCGCTAC CCATCCCGGC TCAGGCCTGG ACGCTTGAGG TGATGGGTCC GGCGACGCTG AAGTTCGCTC CTCGCCTGGC CACTGACTTC CTGCCCCGCT ACCTCGACGG CTCCGAGTGG TGGGGGCAGG GCTTCTCCGA ACCAGAGTCC GGCAGCGACC TGGCCTCGCT TCGGACGCGC GCGACCGACG ACGGCGCGGG CGGGTTCATC GTCAACGGCC AGAAGATCTG GACTAGTCAG GGTGCCACGG CCACGCGGCT ATACGTGCTT GTGCGCACCG GTACGCCGGA AAGCAGGCAT CGCGGGCTGT CAATGCTTCT GATCGACAGC GACGCACCCG GCGTCACATT CCGTCCGATC GCCTTGGCCA GCGGGCGCCG CGAGTTGGCC GAATTGTTCT TCGATGATGT ACGAGTCCCA CCGGAGCGAC TGGTGGGCGA GGTCGGTGGC GGCTGGGCGG TGACGATGTT CTTGATGCAA TATGAGCGCG GCATGTACGG CTACGCGGCA CTAAACAAGG TCATGATGGA GTTGGGCCGC CTGCGGGATT ACATGGTCAA CCGTGCTGTC AGCGCAGCGC AGCGGGAGCG ATTCGCCCGC GTATACGTCG ACGTCACCTC GGCACAGGCC CGTGCTGCCG CTACCGTTCG CAAGCTGGCC GCCGGGCAGG TTGTGGGCCC GGAGAGCAGC ATCGACAAGC TGTTGTGCAG TGAGGCCGAG CGGGAGGCCC ACGACTTGAT CCTCGACGTC ACGCGGGACC ACATGATGGT CGGCACGGGC ACCGGTATCG ACCCGGCGGC GTTCGACACC GCGCGAGCGG GGTGGTGGTA CTCGCGTGCC GCCACGATCA TGGGGGGCAC TGCCGAGGTT CAGCGTGGGA TCATCGCCGA CCACATCTTG GGTTTGCCGA AGGAAAGGCG CGCATCA

Details for MythA.01613.b.A1.GE28794

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MIKANCSNDP PKSPHQHRPQ ATISTPERRS PETPRSTGID NSSLPIPAQA WTLEVMGPAT LKFAPRLATD FLPRYLDGSE WWGQGFSEPE SGSDLASLRT RATDDGAGGF IVNGQKIWTS QGATATRLYV LVRTGTPESR HRGLSMLLID SDAPGVTFRP IALASGRREL AELFFDDVRV PPERLVGEVG GGWAVTMFLM QYERGMYGYA ALNKVMMELG RLRDYMVNRA VSAAQRERFA RVYVDVTSAQ ARAAATVRKL AAGQVVGPES SIDKLLCSEA EREAHDLILD VTRDHMMVGT GTGIDPAAFD TARAGWWYSR AATIMGGTAE VQRGIIADHI LGLPKERRAS

Validated NT Sequence

ttattatgat gcgcgccttt ccttcggcaa acccaagatg tggtcggcga tgatcccacg ctgaacctcg gcagtgcccc ccatgatcgt ggcggcacgc gagtaccacc accccgctcg cgcggtgtcg aacgccgccg ggtcgatacc ggtgcccgtg ccgaccatca tgtggtcccg cgtgacgtcg aggatcaagt cgtgggcctc ccgctcggcc tcactgcaca acagcttgtc gatgctgctc tccgggccca caacctgccc ggcggccagc ttgcgaacgg tagcggcagc acgggcctgt gccgaggtga cgtcgacgta tacgcgggcg aatcgctccc gctgcgctgc gctgacagca cggttgacca tgtaatcccg caggcggccc aactccatca tgaccttgtt tagtgccgcg tagccgtaca tgccgcgctc atattgcatc aagaacatcg tcaccgccca gccgccaccg acctcgccca ccagtcgctc cggtgggact cgtacatcat cgaagaacaa ttcggccaac tcgcggcgcc cgctggccaa ggcgatcgga cggaatgtga cgccgggtgc gtcgctgtcg atcagaagca ttgacagccc gcgatgcctg ctttccggcg taccggtgcg cacaagcacg tatagccgcg tggccgtggc accctgacta gtccagatct tctggccgtt gacgatgaac ccgcccgcgc cgtcgtcggt cgcgcgcgtc cgaagcgagg ccaggtcgct gccggactct ggttcggaga agccctgccc ccaccactcg gagccgtcga ggtagcgggg caggaagtca gtggccaggc gaggagcgaa cttcagcgtc gccggaccca tcacctcaag cgtccaggcc tgagccggga tgggtagcga ggaattgtca atacctgtgg atcggggtgt ttcaggcgac ctccgttccg gtgtgctgat cgtcgcctga gggcggtgtt ggtggggtga tttcggtggg tcgttcgagc agtttgcctt tatcatcgaa ccaggaccct gggtctgagc ttccagggta cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIKANCSND PPKSPHQHRP QATISTPERR SPETPRSTGI DNSSLPIPAQ AWTLEVMGPA TLKFAPRLAT DFLPRYLDGS EWWGQGFSEP ESGSDLASLR TRATDDGAGG FIVNGQKIWT SQGATATRLY VLVRTGTPES RHRGLSMLLI DSDAPGVTFR PIALASGRRE LAELFFDDVR VPPERLVGEV GGGWAVTMFL MQYERGMYGY AALNKVMMEL GRLRDYMVNR AVSAAQRERF ARVYVDVTSA QARAAATVRK LAAGQVVGPE SSIDKLLCSE AEREAHDLIL DVTRDHMMVG TGTGIDPAAF DTARAGWWYS RAATIMGGTA EVQRGIIADH ILGLPKERRA S

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataaaggca aactgctcga acgacccacc gaaatcaccc caccaacacc gccctcaggc gacgatcagc acaccggaac ggaggtcgcc tgaaacaccc cgatccacag gtattgacaa ttcctcgcta cccatcccgg ctcaggcctg gacgcttgag gtgatgggtc cggcgacgct gaagttcgct cctcgcctgg ccactgactt cctgccccgc tacctcgacg gctccgagtg gtgggggcag ggcttctccg aaccagagtc cggcagcgac ctggcctcgc ttcggacgcg cgcgaccgac gacggcgcgg gcgggttcat cgtcaacggc cagaagatct ggactagtca gggtgccacg gccacgcggc tatacgtgct tgtgcgcacc ggtacgccgg aaagcaggca tcgcgggctg tcaatgcttc tgatcgacag cgacgcaccc ggcgtcacat tccgtccgat cgccttggcc agcgggcgcc gcgagttggc cgaattgttc ttcgatgatg tacgagtccc accggagcga ctggtgggcg aggtcggtgg cggctgggcg gtgacgatgt tcttgatgca atatgagcgc ggcatgtacg gctacgcggc actaaacaag gtcatgatgg agttgggccg cctgcgggat tacatggtca accgtgctgt cagcgcagcg cagcgggagc gattcgcccg cgtatacgtc gacgtcacct cggcacaggc ccgtgctgcc gctaccgttc gcaagctggc cgccgggcag gttgtgggcc cggagagcag catcgacaag ctgttgtgca gtgaggccga gcgggaggcc cacgacttga tcctcgacgt cacgcgggac cacatgatgg tcggcacggg caccggtatc gacccggcgg cgttcgacac cgcgcgagcg gggtggtggt actcgcgtgc cgccacgatc atggggggca ctgccgaggt tcagcgtggg atcatcgccg accacatctt gggtttgccg aaggaaaggc gcgcatcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa