SSGCID: Target Page

MythA.01608.a

Ribonucleoside-diphosphate reductase, beta subunit (A0QR94 homolog)

CENTER ID:	MythA.01608.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01608.a.A2.GE29590	potential soluble domain		51	396

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01608.a.A2.PW29490	potential soluble domain		51	396

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1407
RefSeq:	WP_003924929.1
UniProt:	G7CEL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLWGGFPWPS XXXXXXXXXX XXXXPARWIR PSTGSGADRA DIPHDTRCCV HLPPGTPRVV FGYNVPTSEV GTVSEGMKLV NRVSAINWNR LEDDKDAEVW HRLTGNFWLP EKVPVSNDIP SWNTLTDTEK QLTMRVFTGL TLLDTIQGTV GAVSLIPDAL TPHEEAVLTN IAFMESVHAK SYSNIFSTLC STAEIDDAFR WSEENPNLQR KAEIVMQYYR GDEPLKRKVA STLLESFLFY SGFYLPMYWS SRAKLTNTAD MIRLIIRDEA VHGYYIGYKY QRGLAMVEPA KQKELKDYTY DLLFELYDNE VEYTQDLYDD VGLTEDVKKF LRYNANKALM NLGYEALFPK DETDVNPAIL SALAPNADEN HDFFSGSGSS YVIGKAVVTE DEDWDF

NT Sequence

GTGCTGTGGG GCGGGTTCCC CTGGCCGTCC CNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NACCCGCACG CTGGATCCGG CCGAGTACCG GTTCTGGGGC TGACCGGGCG GATATCCCGC ACGACACACG ATGTTGTGTT CACCTCCCGC CGGGTACACC ACGGGTAGTG TTCGGGTACA ACGTCCCGAC GTCCGAGGTG GGTACTGTGA GCGAGGGCAT GAAGCTGGTC AACCGGGTAT CAGCGATCAA CTGGAACCGG CTCGAGGACG ACAAGGACGC CGAGGTCTGG CACCGGCTCA CCGGCAACTT CTGGCTGCCC GAGAAGGTGC CCGTGTCCAA CGACATCCCC TCGTGGAACA CCCTGACCGA CACCGAGAAG CAGTTGACCA TGCGGGTGTT CACCGGTCTG ACCCTGCTGG ACACCATCCA GGGCACCGTC GGCGCGGTCA GCCTCATCCC GGATGCGCTC ACCCCGCACG AGGAAGCGGT CCTGACCAAC ATCGCGTTCA TGGAGTCGGT GCACGCCAAG AGCTACAGCA ACATCTTCTC CACGCTGTGC TCGACGGCCG AGATCGACGA CGCGTTCCGC TGGTCGGAGG AGAACCCGAA CCTGCAGCGC AAGGCCGAGA TCGTCATGCA GTACTACCGC GGGGACGAGC CGCTCAAGCG CAAAGTGGCG TCCACACTGC TGGAGAGCTT TCTGTTCTAC TCCGGCTTCT ATCTGCCGAT GTACTGGTCC AGCCGCGCCA AGCTGACCAA CACCGCCGAC ATGATCCGGC TGATCATCCG CGACGAGGCC GTGCACGGCT ACTACATCGG CTACAAGTAT CAGCGCGGTC TGGCCATGGT GGAGCCGGCC AAGCAGAAGG AGCTCAAGGA CTACACCTAC GACCTGTTGT TCGAGCTCTA CGACAACGAG GTGGAGTACA CCCAGGACCT CTACGACGAC GTCGGGCTGA CCGAGGACGT CAAGAAGTTC CTGCGCTACA ACGCCAACAA GGCCCTGATG AACCTCGGCT ACGAGGCCCT GTTCCCCAAG GACGAGACCG ACGTCAACCC GGCGATCCTG TCGGCGCTGG CGCCCAACGC CGACGAGAAC CACGACTTCT TCTCCGGGTC GGGTAGCTCC TATGTGATCG GGAAGGCCGT CGTCACCGAG GACGAGGACT GGGACTTC

Details for MythA.01608.a.A2.GE29590

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MHLPPGTPRV VFGYNVPTSE VGTVSEGMKL VNRVSAINWN RLEDDKDAEV WHRLTGNFWL PEKVPVSNDI PSWNTLTDTE KQLTMRVFTG LTLLDTIQGT VGAVSLIPDA LTPHEEAVLT NIAFMESVHA KSYSNIFSTL CSTAEIDDAF RWSEENPNLQ RKAEIVMQYY RGDEPLKRKV ASTLLESFLF YSGFYLPMYW SSRAKLTNTA DMIRLIIRDE AVHGYYIGYK YQRGLAMVEP AKQKELKDYT YDLLFELYDN EVEYTQDLYD DVGLTEDVKK FLRYNANKAL MNLGYEALFP KDETDVNPAI LSALAPNADE NHDFFSGSGS SYVIGKAVVT EDEDW

Validated NT Sequence

cccagtcctc gtcctcggtg acgacggcct tcccgatcac ataggagcta cccgacccgg agaagaagtc gtggttctcg tcggcgttgg gcgccagcgc cgacaggatc gccgggttga cgtcggtctc gtccttgggg aacagggcct cgtagccgag gttcatcagg gccttgttgg cgttgtagcg caggaacttc ttgacgtcct cggtcagccc gacgtcgtcg tagaggtcct gggtgtactc cacctcgttg tcgtagagct cgaacaacag gtcgtaggtg tagtccttga gctccttctg cttggccggc tccaccatgg ccagaccgcg ctgatacttg tagccgatgt agtagccgtg cacggcctcg tcgcggatga tcagccggat catgtcggcg gtgttggtca gcttggcgcg gctggaccag tacatcggca gatagaagcc ggagtagaac agaaagctct ccagcagtgt ggacgccact ttgcgcttga gcggctcgtc cccgcggtag tactgcatga cgatctcggc cttgcgctgc aggttcgggt tctcctccga ccagcggaac gcgtcgtcga tctcggccgt cgagcacagc gtggagaaga tgttgctgta gctcttggcg tgcaccgact ccatgaacgc gatgttggtc aggaccgctt cctcgtgcgg ggtgagcgca tccgggatga ggctgaccgc gccgacggtg ccctggatgg tgtccagcag ggtcagaccg gtgaacaccc gcatggtcaa ctgcttctcg gtgtcggtca gggtgttcca cgaggggatg tcgttggaca cgggcacctt ctcgggcagc cagaagttgc cggtgagccg gtgccagacc tcggcgtcct tgtcgtcctc gagccggttc cagttgatcg ctgatacccg gttgaccagc ttcatgccct cgctcacagt acccacctcg gacgtcggga cgttgtaccc gaacactacc cgtggtgtac ccggcgggag gtgcatcgaa ccaggaccct gggtctgagc ttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHLPPGTPR VVFGYNVPTS EVGTVSEGMK LVNRVSAINW NRLEDDKDAE VWHRLTGNFW LPEKVPVSND IPSWNTLTDT EKQLTMRVFT GLTLLDTIQG TVGAVSLIPD ALTPHEEAVL TNIAFMESVH AKSYSNIFST LCSTAEIDDA FRWSEENPNL QRKAEIVMQY YRGDEPLKRK VASTLLESFL FYSGFYLPMY WSSRAKLTNT ADMIRLIIRD EAVHGYYIGY KYQRGLAMVE PAKQKELKDY TYDLLFELYD NEVEYTQDLY DDVGLTEDVK KFLRYNANKA LMNLGYEALF PKDETDVNPA ILSALAPNAD ENHDFFSGSG SSYVIGKAVV TEDEDWDF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacctcccg ccgggtacac cacgggtagt gttcgggtac aacgtcccga cgtccgaggt gggtactgtg agcgagggca tgaagctggt caaccgggta tcagcgatca actggaaccg gctcgaggac gacaaggacg ccgaggtctg gcaccggctc accggcaact tctggctgcc cgagaaggtg cccgtgtcca acgacatccc ctcgtggaac accctgaccg acaccgagaa gcagttgacc atgcgggtgt tcaccggtct gaccctgctg gacaccatcc agggcaccgt cggcgcggtc agcctcatcc cggatgcgct caccccgcac gaggaagcgg tcctgaccaa catcgcgttc atggagtcgg tgcacgccaa gagctacagc aacatcttct ccacgctgtg ctcgacggcc gagatcgacg acgcgttccg ctggtcggag gagaacccga acctgcagcg caaggccgag atcgtcatgc agtactaccg cggggacgag ccgctcaagc gcaaagtggc gtccacactg ctggagagct ttctgttcta ctccggcttc tatctgccga tgtactggtc cagccgcgcc aagctgacca acaccgccga catgatccgg ctgatcatcc gcgacgaggc cgtgcacggc tactacatcg gctacaagta tcagcgcggt ctggccatgg tggagccggc caagcagaag gagctcaagg actacaccta cgacctgttg ttcgagctct acgacaacga ggtggagtac acccaggacc tctacgacga cgtcgggctg accgaggacg tcaagaagtt cctgcgctac aacgccaaca aggccctgat gaacctcggc tacgaggccc tgttccccaa ggacgagacc gacgtcaacc cggcgatcct gtcggcgctg gcgcccaacg ccgacgagaa ccacgacttc ttctccgggt cgggtagctc ctatgtgatc gggaaggccg tcgtcaccga ggacgaggac tgggacttca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MythA.01608.a.A2.PW29490

PURIFICATION DATe:	8/31/2010
CONCENTRATION:	17.94mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCACCTCCCG CCGGGTACAC CACGGGTAGT GTTCGGGTAC AACGTCCCGA CGTCCGAGGT GGGTACTGTG AGCGAGGGCA TGAAGCTGGT CAACCGGGTA TCAGCGATCA ACTGGAACCG GCTCGAGGAC GACAAGGACG CCGAGGTCTG GCACCGGCTC ACCGGCAACT TCTGGCTGCC CGAGAAGGTG CCCGTGTCCA ACGACATCCC CTCGTGGAAC ACCCTGACCG ACACCGAGAA GCAGTTGACC ATGCGGGTGT TCACCGGTCT GACCCTGCTG GACACCATCC AGGGCACCGT CGGCGCGGTC AGCCTCATCC CGGATGCGCT CACCCCGCAC GAGGAAGCGG TCCTGACCAA CATCGCGTTC ATGGAGTCGG TGCACGCCAA GAGCTACAGC AACATCTTCT CCACGCTGTG CTCGACGGCC GAGATCGACG ACGCGTTCCG CTGGTCGGAG GAGAACCCGA ACCTGCAGCG CAAGGCCGAG ATCGTCATGC AGTACTACCG CGGGGACGAG CCGCTCAAGC GCAAAGTGGC GTCCACACTG CTGGAGAGCT TTCTGTTCTA CTCCGGCTTC TATCTGCCGA TGTACTGGTC CAGCCGCGCC AAGCTGACCA ACACCGCCGA CATGATCCGG CTGATCATCC GCGACGAGGC CGTGCACGGC TACTACATCG GCTACAAGTA TCAGCGCGGT CTGGCCATGG TGGAGCCGGC CAAGCAGAAG GAGCTCAAGG ACTACACCTA CGACCTGTTG TTCGAGCTCT ACGACAACGA GGTGGAGTAC ACCCAGGACC TCTACGACGA CGTCGGGCTG ACCGAGGACG TCAAGAAGTT CCTGCGCTAC AACGCCAACA AGGCCCTGAT GAACCTCGGC TACGAGGCCC TGTTCCCCAA GGACGAGACC GACGTCAACC CGGCGATCCT GTCGGCGCTG GCGCCCAACG CCGACGAGAA CCACGACTTC TTCTCCGGGT CGGGTAGCTC CTATGTGATC GGGAAGGCCG TCGTCACCGA GGACGAGGAC TGGGACTTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A