SSGCID: Target Page

MythA.01365.b

L-xylulose reductase (A0QXD6 homolog)

CENTER ID:	MythA.01365.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01365.b.A1.GE28787	Full length( MythA.01365.b )		1	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01365.b.A1.PS00640	Full length( MythA.01365.b )		1	279

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.3413
RefSeq:	WP_003926944.1
UniProt:	G7CJ82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCRGAGPRAW CWICEAKVSS QVSMGDILTG QRALITGASE GIGFGIAQAM LEAGADVMIA ARRRSKLESA QQELGALARP NQRVEIVEVD VTQPDSVQKM FEHCDETFGT LNIFVANAGS GSMVPFLEMT EEIWRSTLEL NLGGTFRTCQ HAARRMVDRA DRDANAAILV VSSIRALGTR PGTIHYATTK AGLNQFVRVA AYELAGSGVR XXXXXXXXXA TPMALQRNPD VFTEMTATVP MGRAGTPADM GAAAVYLCSP AANFVTGTNH IVDGGESLY

NT Sequence

GTGTGTCGCG GTGCCGGACC CCGCGCCTGG TGCTGGATCT GTGAGGCGAA AGTGAGCAGT CAAGTGAGCA TGGGAGACAT CCTCACCGGT CAACGGGCCT TGATCACCGG CGCGAGCGAG GGCATCGGAT TCGGCATCGC GCAGGCGATG CTCGAGGCCG GAGCGGATGT GATGATCGCC GCACGCCGGC GATCCAAACT CGAGAGCGCA CAACAGGAAC TGGGCGCGCT GGCACGACCG AATCAGCGGG TCGAGATCGT CGAAGTCGAT GTCACCCAAC CTGATTCGGT CCAGAAGATG TTCGAACACT GTGATGAGAC TTTCGGGACG CTGAACATCT TCGTGGCCAA CGCGGGAAGC GGGTCGATGG TGCCGTTCCT CGAGATGACC GAGGAGATCT GGCGATCCAC GCTGGAACTC AACCTCGGCG GCACGTTCCG CACCTGCCAG CACGCGGCCC GCCGCATGGT CGACCGCGCG GACCGGGACG CCAACGCGGC GATCCTGGTG GTGTCCTCGA TCCGTGCCCT GGGCACCAGG CCGGGCACCA TCCACTACGC CACCACCAAG GCCGGTCTCA ACCAGTTCGT CCGCGTCGCC GCCTATGAGC TCGCCGGGTC CGGGGTGCGG NNNNNNNNNN NNNNNNNNNN NNNNNNNGCC ACTCCGATGG CCCTGCAACG CAATCCGGAT GTCTTCACCG AGATGACCGC GACGGTGCCG ATGGGACGGG CAGGCACCCC GGCCGACATG GGCGCAGCCG CCGTCTACCT GTGCTCGCCG GCGGCCAATT TCGTGACCGG AACCAACCAC ATCGTCGATG GTGGGGAATC CCTGTAC

Details for MythA.01365.b.A1.GE28787

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVCRGAGPR AWCWICEAKV SSQVSMGDIL TGQRALITGA SEGIGFGIAQ AMLEAGADVM IAARRRSKLE SAQQELGALA RPNQRVEIVE VDVTQPDSVQ KMFEHCDETF GTLNIFVANA GSGSMVPFLE MTEEIWRSTL ELNLGGTFRT CQHAARRMVD RADRDANAAI LVVSSIRALG TRPGTIHYAT TKAGLNQFVR VAAYELAGSG VRINALSPGI TATPMALQRN PDVFTEMTAT VPMGRAGTPA DMGAAAVYLC SPAANFVTGT NHIVDGGESL Y

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt gtcgcggtgc cggaccccgc gcctggtgct ggatctgtga ggcgaaagtg agcagtcaag tgagcatggg agacatcctc accggtcaac gggccttgat caccggcgcg agcgagggca tcggattcgg catcgcgcag gcgatgctcg aggccggagc ggatgtgatg atcgccgcac gccggcgatc caaactcgag agcgcacaac aggaactggg cgcgctggca cgaccgaatc agcgggtcga gatcgtcgaa gtcgatgtca cccaacctga ttcggtccag aagatgttcg aacactgtga tgagactttc gggacgctga acatcttcgt ggccaacgcg ggaagcgggt cgatggtgcc gttcctcgag atgaccgagg agatctggcg atccacgctg gaactcaacc tcggcggcac gttccgcacc tgccagcacg cggcccgccg catggtcgac cgcgcggacc gggacgccaa cgcggcgatc ctggtggtgt cctcgatccg tgccctgggc accaggccgg gcaccatcca ctacgccacc accaaggccg gtctcaacca gttcgtccgc gtcgccgcct atgagctcgc cgggtcagga gtgcggatca acgcgctctc accgggcatc accgccactc cgatggccct gcaacgcaat ccggatgtct tcaccgagat gaccgcgacg gtgccgatgg gacgggcagg caccccggcc gacatgggcg cagccgccgt ctacctgtgc tcgccggcgg ccaatttcgt gaccggaacc aaccacatcg tcgatggtgg ggaatccctg tactaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVCRGAGPR AWCWICEAKV SSQVSMGDIL TGQRALITGA SEGIGFGIAQ AMLEAGADVM IAARRRSKLE SAQQELGALA RPNQRVEIVE VDVTQPDSVQ KMFEHCDETF GTLNIFVANA GSGSMVPFLE MTEEIWRSTL ELNLGGTFRT CQHAARRMVD RADRDANAAI LVVSSIRALG TRPGTIHYAT TKAGLNQFVR VAAYELAGSG VRXXXXXXXX XATPMALQRN PDVFTEMTAT VPMGRAGTPA DMGAAAVYLC SPAANFVTGT NHIVDGGESL Y

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgtcgc ggtgccggac cccgcgcctg gtgctggatc tgtgaggcga aagtgagcag tcaagtgagc atgggagaca tcctcaccgg tcaacgggcc ttgatcaccg gcgcgagcga gggcatcgga ttcggcatcg cgcaggcgat gctcgaggcc ggagcggatg tgatgatcgc cgcacgccgg cgatccaaac tcgagagcgc acaacaggaa ctgggcgcgc tggcacgacc gaatcagcgg gtcgagatcg tcgaagtcga tgtcacccaa cctgattcgg tccagaagat gttcgaacac tgtgatgaga ctttcgggac gctgaacatc ttcgtggcca acgcgggaag cgggtcgatg gtgccgttcc tcgagatgac cgaggagatc tggcgatcca cgctggaact caacctcggc ggcacgttcc gcacctgcca gcacgcggcc cgccgcatgg tcgaccgcgc ggaccgggac gccaacgcgg cgatcctggt ggtgtcctcg atccgtgccc tgggcaccag gccgggcacc atccactacg ccaccaccaa ggccggtctc aaccagttcg tccgcgtcgc cgcctatgag ctcgccgggt ccggggtgcg gnnnnnnnnn nnnnnnnnnn nnnnnnnngc cactccgatg gccctgcaac gcaatccgga tgtcttcacc gagatgaccg cgacggtgcc gatgggacgg gcaggcaccc cggccgacat gggcgcagcc gccgtctacc tgtgctcgcc ggcggccaat ttcgtgaccg gaaccaacca catcgtcgat ggtggggaat ccctgtacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MythA.01365.b.A1.PS00640

PURIFICATION DATe:	6/11/2010
CONCENTRATION:	5.66mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVCRGAGPR AWCWICEAKV SSQVSMGDIL TGQRALITGA SEGIGFGIAQ AMLEAGADVM IAARRRSKLE SAQQELGALA RPNQRVEIVE VDVTQPDSVQ KMFEHCDETF GTLNIFVANA GSGSMVPFLE MTEEIWRSTL ELNLGGTFRT CQHAARRMVD RADRDANAAI LVVSSIRALG TRPGTIHYAT TKAGLNQFVR VAAYELAGSG VRXXXXXXXX XATPMALQRN PDVFTEMTAT VPMGRAGTPA DMGAAAVYLC SPAANFVTGT NHIVDGGESL Y

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTGTCGC GGTGCCGGAC CCCGCGCCTG GTGCTGGATC TGTGAGGCGA AAGTGAGCAG TCAAGTGAGC ATGGGAGACA TCCTCACCGG TCAACGGGCC TTGATCACCG GCGCGAGCGA GGGCATCGGA TTCGGCATCG CGCAGGCGAT GCTCGAGGCC GGAGCGGATG TGATGATCGC CGCACGCCGG CGATCCAAAC TCGAGAGCGC ACAACAGGAA CTGGGCGCGC TGGCACGACC GAATCAGCGG GTCGAGATCG TCGAAGTCGA TGTCACCCAA CCTGATTCGG TCCAGAAGAT GTTCGAACAC TGTGATGAGA CTTTCGGGAC GCTGAACATC TTCGTGGCCA ACGCGGGAAG CGGGTCGATG GTGCCGTTCC TCGAGATGAC CGAGGAGATC TGGCGATCCA CGCTGGAACT CAACCTCGGC GGCACGTTCC GCACCTGCCA GCACGCGGCC CGCCGCATGG TCGACCGCGC GGACCGGGAC GCCAACGCGG CGATCCTGGT GGTGTCCTCG ATCCGTGCCC TGGGCACCAG GCCGGGCACC ATCCACTACG CCACCACCAA GGCCGGTCTC AACCAGTTCG TCCGCGTCGC CGCCTATGAG CTCGCCGGGT CCGGGGTGCG GNNNNNNNNN NNNNNNNNNN NNNNNNNNGC CACTCCGATG GCCCTGCAAC GCAATCCGGA TGTCTTCACC GAGATGACCG CGACGGTGCC GATGGGACGG GCAGGCACCC CGGCCGACAT GGGCGCAGCC GCCGTCTACC TGTGCTCGCC GGCGGCCAAT TTCGTGACCG GAACCAACCA CATCGTCGAT GGTGGGGAAT CCCTGTACAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA