SSGCID: Target Page

MythA.01263.d

Isovaleryl-CoA dehydrogenase (A0QTW7 homolog)

CENTER ID:	MythA.01263.d
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01263.d.A1.GE28777	Full length( MythA.01263.d )		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.238
RefSeq:	WP_003923673.1
UniProt:	G7CBA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAGKFFSSI GMSEHGAGSD LAAVQTKAVR TDGGWVLNGT KVWTSGAHKA HQVVVLARTS PPDPQKRHAG FSQFLVPCDA KGVRIEPILL MNGDHHFNEV SFEDVFIPDA DVLGEIGNGW HQVTAELSFE RSGPERILST VPLIVAAIRL LGNTPGTDDT TARIVGDLLG RLISLRQLSV WVARTLSEHK DAANQAALVK DLGTRFEQDS IEVIADVLDR VSPTPQIAAL TATAILHKPI FTLRGGTNEV LRGVVARGMG LR

NT Sequence

ATCGCCGCCG GGAAGTTCTT CTCCTCCATC GGGATGAGCG AACACGGCGC GGGGTCGGAC CTGGCCGCCG TGCAGACGAA GGCGGTCAGG ACCGACGGCG GCTGGGTGCT CAACGGCACC AAGGTGTGGA CCAGCGGTGC GCACAAGGCC CATCAGGTCG TCGTGCTGGC CCGCACCAGC CCGCCGGATC CGCAGAAGCG GCACGCCGGG TTCAGCCAGT TCCTGGTGCC GTGCGACGCC AAGGGCGTGC GGATCGAACC GATCCTGCTG ATGAACGGCG ACCACCACTT CAACGAGGTC TCCTTCGAAG ACGTGTTCAT CCCGGACGCG GATGTGCTCG GCGAGATCGG CAACGGCTGG CACCAGGTGA CCGCCGAGCT GTCCTTCGAG CGCAGCGGGC CCGAGCGGAT CCTGTCGACG GTGCCGCTGA TCGTCGCGGC GATCCGCCTG CTGGGCAACA CCCCCGGCAC CGATGACACC ACCGCCAGGA TCGTGGGTGA CCTTCTGGGA CGGCTGATCT CACTGCGGCA GCTGTCGGTG TGGGTGGCGC GCACGCTGTC CGAGCACAAG GACGCCGCCA ACCAGGCCGC ACTGGTCAAG GATCTGGGCA CACGCTTCGA ACAGGATTCG ATCGAGGTGA TCGCCGATGT GCTCGACCGG GTGTCGCCGA CACCTCAGAT CGCGGCGCTG ACCGCCACCG CGATCCTGCA CAAGCCGATC TTCACGCTGC GCGGCGGCAC CAACGAGGTG CTGCGCGGCG TCGTCGCACG AGGAATGGGG TTGCGA

Details for MythA.01263.d.A1.GE28777

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	89

Validated AA Sequence

MIAAGKFFSS IGMSEHGAGS DLAAVQTKAV RTDGGWVLNG TKVWTSGAHK AHQVVVLART SPPDPQKRHA GFSQFLVPCD AKGVRIEPIL LMNGDHHFNE VSFEDVFIPD AXVLGEIGNG WHQVTAELSF ERXGPXRXXS TVPLIVXXIR LLGNTPGTDD TTARIVGDLL XXLISLRQXS VWVARTLSEH KDAANQAALV KDLGTRFEQD SIEVXXDVLX RVSPTPQIAA LTATAILHKP IFTLRGGTNE VLRGV

Validated NT Sequence

gacgacgccg cgcagcacct cgttggtgcc gccgcgcagc gtgaagatcg gcttgtgcag gatcgcggtg gcggtcagcg ccgcgatctg aggtgtcggc gacacccggn cgagcacatc gncnatcacc tcgatcgaat cctgttcgaa gcgtgtgccc agatccttga ccagtgcggc ctggttggcg gcgtccttgt gctcggacag cgtgcgcgcc acccacaccg acngctgccg cagtgagatc agncnnncca gaaggtcacc cacgatcctg gcggtggtgt catcggtgcc gggggtgttg cccagcaggc ggatcgncgn gacgatcagc ggcaccgtcg annnnnnccg ctngggcccg ntgcgctcga aggacagctc ggcggtcacc tggtgccagc cgttgccgat ctcgccgagc acatncgcgt ccgggatgaa cacgtcttcg aaggagacct cgttgaagtg gtggtcgccg ttcatcagca ggatcggttc gatccgcacg cccttggcgt cgcacggcac caggaactgg ctgaacccgg cgtgccgctt ctgcggatcc ggcgggctgg tgcgggccag cacgacgacc tgatgggcct tgtgcgcacc gctggtccac accttggtgc cgttgagcac ccagccgccg tcggtcctga ccgccttcgt ctgcacggcg gccaggtccg accccgcgcc gtgttcgctc atcccgatgg aggagaagaa cttcccggcg gcgatcatcg aaccaggacc ctgggtctga gct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIAAGKFFS SIGMSEHGAG SDLAAVQTKA VRTDGGWVLN GTKVWTSGAH KAHQVVVLAR TSPPDPQKRH AGFSQFLVPC DAKGVRIEPI LLMNGDHHFN EVSFEDVFIP DADVLGEIGN GWHQVTAELS FERSGPERIL STVPLIVAAI RLLGNTPGTD DTTARIVGDL LGRLISLRQL SVWVARTLSE HKDAANQAAL VKDLGTRFEQ DSIEVIADVL DRVSPTPQIA ALTATAILHK PIFTLRGGTN EVLRGVVARG MGLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgccgcc gggaagttct tctcctccat cgggatgagc gaacacggcg cggggtcgga cctggccgcc gtgcagacga aggcggtcag gaccgacggc ggctgggtgc tcaacggcac caaggtgtgg accagcggtg cgcacaaggc ccatcaggtc gtcgtgctgg cccgcaccag cccgccggat ccgcagaagc ggcacgccgg gttcagccag ttcctggtgc cgtgcgacgc caagggcgtg cggatcgaac cgatcctgct gatgaacggc gaccaccact tcaacgaggt ctccttcgaa gacgtgttca tcccggacgc ggatgtgctc ggcgagatcg gcaacggctg gcaccaggtg accgccgagc tgtccttcga gcgcagcggg cccgagcgga tcctgtcgac ggtgccgctg atcgtcgcgg cgatccgcct gctgggcaac acccccggca ccgatgacac caccgccagg atcgtgggtg accttctggg acggctgatc tcactgcggc agctgtcggt gtgggtggcg cgcacgctgt ccgagcacaa ggacgccgcc aaccaggccg cactggtcaa ggatctgggc acacgcttcg aacaggattc gatcgaggtg atcgccgatg tgctcgaccg ggtgtcgccg acacctcaga tcgcggcgct gaccgccacc gcgatcctgc acaagccgat cttcacgctg cgcggcggca ccaacgaggt gctgcgcggc gtcgtcgcac gaggaatggg gttgcgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa