MythA.01117.a

Putative acyl-CoA dehydrogenase (A0R4P4 homolog)

CENTER ID:	MythA.01117.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01117.a.A1.GE28769	Full length( MythA.01117.a )		1	519

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.2789
RefSeq:	WP_003926319.1
UniProt:	G7CGU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLVSAIQGC PRAFVDGLEC LLLQLRRDGI ELDHRVVVIV ELEDLRGDAH ADRVALTTVP VHFHSHRNLL ALAVAVCAAA DDGQNTTRRT PASAHCHLDR QTRTCYNRVR IWVTPPVEAK DTHADRLHAR AGGVAPRTAG LLLETDDARP GGSAELRRRR IRARRRLPRD RRPDGSRRLA RAGLAEGIRR GGXXXXXXXX XXXXXAAIAG APVPFLTINS VAPTIMHFGT EEQKKFFLPK IAAGELHFSI GYSEPGAGTD LASLRTTAVR DGDEYVINGQ KMWTSLIQYA DYVWLAVRTN PEAKKHRGIS MLIVPTTAEG FSWTPVHTMA GVDTSATYYQ DVRVPVSNRV GEENEGWKLV TNQLNHERVA LVSAQPIFLA LDGVREWAQS THISPDGRGP RVIDAEWVQL NLARVHAKAE VLKLINWELA SSDAAAPSPA DASAAKVFGT ELATEAYRLL MEVLGTRATL RPDSPGALLR GRIERMHRSC LILTFGGGTN EVQRDIIGMV ALGLPRVNR

NT Sequence

ATACCTCTAG TCTCTGCGAT TCAGGGCTGC CCGAGGGCAT TCGTCGATGG CCTGGAGTGC CTTCTGCTCC AACTCCGCCG GGATGGGATC GAGCTTGACC ACCGCGTAGT CGTCATCGTC GAGCTCGAAG ATCTCCGGGG AGATGCCCAC GCAGATCGCG TTGCCCTCAC AACGGTCCCG GTCCACTTCC ACTCGCATCG CAACCTCCTT GCCCTCGCGG TTGCCGTCTG TGCAGCCGCG GATGATGGTC AGAATACGAC CCGCCGGACC CCCGCCTCGG CGCATTGCCA TCTGGACCGC CAGACTAGAA CGTGTTACAA CCGGGTGAGA ATCTGGGTCA CCCCTCCCGT CGAGGCAAAG GACACTCATG CGGATCGGCT ACACGCCCGA GCAGGAGGAG TTGCGCCGCG AACTGCGGGC CTACTTCTCG AAACTGATGA CGCCCGACCG GGTGGAAGCG CTGAACTCCG CCGAAGGCGA ATACGGGCGC GGCGACGTCT ACCGCGAGAC CGTCGCCCAG ATGGGTCGCG ACGGTTGGCT CGCGCTGGGC TGGCCGAAGG AATACGGCGG GGCGGNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGCCGC GATCGCCGGC GCACCGGTGC CGTTCCTGAC CATCAACAGC GTCGCGCCGA CGATCATGCA CTTCGGCACC GAGGAGCAGA AGAAGTTCTT CCTGCCCAAG ATCGCCGCGG GTGAGCTGCA TTTCTCGATC GGTTACTCCG AGCCGGGCGC GGGCACCGAC CTGGCCTCGC TGCGCACCAC CGCGGTGCGC GACGGCGACG AGTACGTGAT CAACGGTCAG AAGATGTGGA CCAGTCTGAT CCAGTACGCC GACTACGTGT GGCTGGCGGT CCGCACCAAC CCGGAAGCCA AGAAGCACCG CGGAATCTCG ATGCTGATCG TGCCCACCAC CGCGGAGGGC TTCTCCTGGA CCCCGGTGCA CACCATGGCC GGTGTCGACA CCAGCGCCAC CTACTACCAG GACGTGCGGG TGCCGGTGAG CAACCGCGTC GGCGAGGAGA ACGAGGGCTG GAAGCTGGTC ACCAATCAGC TCAACCACGA GCGCGTGGCG CTCGTCTCCG CCCAACCCAT CTTCCTGGCG CTCGACGGGG TCCGGGAATG GGCGCAGAGC ACTCACATCT CGCCGGACGG ACGGGGCCCC CGGGTGATCG ACGCCGAGTG GGTGCAGCTG AACCTGGCCC GTGTGCACGC CAAGGCCGAG GTGCTCAAGC TGATCAACTG GGAACTGGCG TCCAGCGACG CGGCGGCGCC CTCCCCGGCG GACGCGTCGG CGGCCAAGGT GTTCGGCACC GAGCTGGCCA CCGAGGCGTA CCGGCTGCTG ATGGAGGTGC TGGGCACCAG GGCGACACTG CGCCCGGACT CCCCCGGCGC GCTGCTGCGG GGGCGGATCG AACGGATGCA CCGCAGCTGC CTGATCCTGA CCTTCGGCGG CGGCACCAAC GAGGTTCAGC GCGACATCAT CGGCATGGTC GCGCTCGGCC TGCCCAGGGT GAACCGG

Details for MythA.01117.a.A1.GE28769

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/5/2010
EXPECTED MW:	59kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIPLVSAIQ GCPRAFVDGL ECLLLQLRRD GIELDHRVVV IVELEDLRGD AHADRVALTT VPVHFHSHRN LLALAVAVCA AADDGQNTTR RTPASAHCHL DRQTRTCYNR VRIWVTPPVE AKDTHADRLH ARAGGVAPRT AGLLLETDDA RPGGSAELRR RRIRARRRLP RDRRPDGSRR LARAGLAEGI RRGXXXXXXX XXXXXXXAAI AGAPVPFLTI NSVAPTIMHF GTEEQKKFFL PKIAAGELHF SIGYSEPGAG TDLASLRTTA VRDGDEYVIN GQKMWTSLIQ YADYVWLAVR TNPEAKKHRG ISMLIVPTTA EGFSWTPVHT MAGVDTSATY YQDVRVPVSN RVGEENEGWK LVTNQLNHER VALVSAQPIF LALDGVREWA QSTHISPDGR GPRVIDAEWV QLNLARVHAK AEVLKLINWE LASSDAAAPS PADASAAKVF GTELATEAYR LLMEVLGTRA TLRPDSPGAL LRGRIERMHR SCLILTFGGG TNEVQRDIIG MVALGLPRVN R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatacctcta gtctctgcga ttcagggctg cccgagggca ttcgtcgatg gcctggagtg ccttctgctc caactccgcc gggatgggat cgagcttgac caccgcgtag tcgtcatcgt cgagctcgaa gatctccggg gagatgccca cgcagatcgc gttgccctca caacggtccc ggtccacttc cactcgcatc gcaacctcct tgccctcgcg gttgccgtct gtgcagccgc ggatgatggt cagaatacga cccgccggac ccccgcctcg gcgcattgcc atctggaccg ccagactaga acgtgttaca accgggtgag aatctgggtc acccctcccg tcgaggcaaa ggacactcat gcggatcggc tacacgcccg agcaggagga gttgcgccgc gaactgcggg cctacttctc gaaactgatg acgcccgacc gggtggaagc gctgaactcc gccgaaggcg aatacgggcg cggcgacgtc taccgcgaga ccgtcgccca gatgggtcgc gacggttggc tcgcgctggg ctggccgaag gaatacggcg gggcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggccg cgatcgccgg cgcaccggtg ccgttcctga ccatcaacag cgtcgcgccg acgatcatgc acttcggcac cgaggagcag aagaagttct tcctgcccaa gatcgccgcg ggtgagctgc atttctcgat cggttactcc gagccgggcg cgggcaccga cctggcctcg ctgcgcacca ccgcggtgcg cgacggcgac gagtacgtga tcaacggtca gaagatgtgg accagtctga tccagtacgc cgactacgtg tggctggcgg tccgcaccaa cccggaagcc aagaagcacc gcggaatctc gatgctgatc gtgcccacca ccgcggaggg cttctcctgg accccggtgc acaccatggc cggtgtcgac accagcgcca cctactacca ggacgtgcgg gtgccggtga gcaaccgcgt cggcgaggag aacgagggct ggaagctggt caccaatcag ctcaaccacg agcgcgtggc gctcgtctcc gcccaaccca tcttcctggc gctcgacggg gtccgggaat gggcgcagag cactcacatc tcgccggacg gacggggccc ccgggtgatc gacgccgagt gggtgcagct gaacctggcc cgtgtgcacg ccaaggccga ggtgctcaag ctgatcaact gggaactggc gtccagcgac gcggcggcgc cctccccggc ggacgcgtcg gcggccaagg tgttcggcac cgagctggcc accgaggcgt accggctgct gatggaggtg ctgggcacca gggcgacact gcgcccggac tcccccggcg cgctgctgcg ggggcggatc gaacggatgc accgcagctg cctgatcctg accttcggcg gcggcaccaa cgaggttcag cgcgacatca tcggcatggt cgcgctcggc ctgcccaggg tgaaccggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa