SSGCID: Target Page

MythA.01097.b

Short-chain dehydrogenase/reductase SDR (A0QWA4 homolog)

CENTER ID:	MythA.01097.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01097.b.A1.GE28767	Full length( MythA.01097.b )		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2350
RefSeq:	WP_003925874.1
UniProt:	G7CK17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSRCAPRCA PPSRRRWCRG CSRCWTTPRR RPPKGSRPVR ITDRVFVVTG SGNGMGREVA VELGRRGAHV AAVDIDREGL AGTAELVRAT GARITTHVVD VTDDNAVAAL PAAVLDAHGQ VDGLVNIAGI AQRFALFSDL GAEALDRVPV VNFTGTVRMC RAFLPILLAR PEANITNMSS LSALVPFASQ LLYSASKAAV KQFSEGLDAE LVGTDVRVVT VFPGNVATEL AKNSGVEMLD AGSRRVYATS PEAAGRKIVA GIAXDRYRVI IGADAHVLHA LARIAXXXXX XXXXXARLVA RQITSVLREQ

NT Sequence

ATGCCATCCC GCTGTGCCCC GCGTTGCGCA CCGCCATCGA GACGGCGGTG GTGCAGGGGT TGCTCCCGGT GCTGGACGAC TCCGCGCCGC CGACCACCGA AGGGGAGCCG TCCCGTGCGC ATCACCGATA GGGTGTTCGT CGTCACCGGT TCCGGCAACG GCATGGGGCG GGAGGTCGCC GTGGAGCTGG GCCGGCGCGG GGCACATGTC GCCGCCGTCG ACATCGATCG GGAAGGGCTC GCCGGCACGG CGGAACTGGT TCGGGCCACC GGAGCGCGGA TCACCACCCA CGTCGTGGAC GTCACCGACG ACAATGCGGT GGCGGCCCTG CCCGCTGCGG TGCTCGACGC GCACGGGCAG GTCGACGGTC TGGTCAACAT CGCCGGGATC GCGCAACGGT TCGCGTTGTT CTCCGACCTC GGCGCCGAGG CCCTCGACCG GGTGCCGGTG GTGAACTTCA CCGGCACCGT GCGGATGTGC CGGGCGTTCC TGCCGATCCT GCTGGCGCGT CCGGAGGCCA ACATCACCAA CATGTCGAGC CTGTCGGCTC TGGTGCCGTT CGCCAGTCAG CTGCTCTACA GCGCCAGCAA GGCCGCGGTG AAACAGTTCA GCGAAGGCCT GGACGCCGAA CTCGTCGGTA CCGACGTCCG TGTCGTGACC GTGTTCCCGG GCAACGTCGC CACCGAACTC GCGAAGAACT CCGGGGTGGA GATGCTCGAC GCCGGCAGCC GACGGGTGTA CGCCACCAGC CCCGAGGCGG CCGGCAGGAA GATCGTCGCC GGTATCGCGA GNGACCGGTA CCGGGTGATC ATCGGCGCGG ACGCCCATGT GTTGCATGCC CTTGCGCGGA TCGCNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCACG GCTGGTGGCC AGACAGATCA CATCCGTTCT CCGGGAGCAG

Details for MythA.01097.b.A1.GE28767

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPSRCAPRC APPSRRRWCR GCSRCWTTPR RRPPKGSRPV RITDRVFVVT GSGNGMGREV AVELGRRGAH VAAVDIDREG LAGTAELVRA TGARITTHVV DVTDDNAVAA LPAAVLDAHG QVDGLVNIAG IAQRFALFSD LGAEALDRVP VVNFTGTVRM CRAFLPILLA RPEANITNMS SLSALVPFAS QLLYSASKAA VKQFSEGLDA ELVGTDVRVV TVFPGNVATE LAKNSGVEML DAGSRRVYAT SPEAAGRKIV AGIARDRYRV IIGADAHVLH ALARIAPRRT ARLVARQITS VLR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccat cccgctgtgc cccgcgttgc gcaccgccat cgagacggcg gtggtgcagg ggttgctccc ggtgctggac gactccgcgc cgccgaccac cgaaggggag ccgtcccgtg cgcatcaccg atagggtgtt cgtcgtcacc ggttccggca acggcatggg gcgggaggtc gccgtggagc tgggccggcg cggggcacat gtcgccgccg tcgacatcga tcgggaaggg ctcgccggca cggcggaact ggttcgggcc accggagcgc ggatcaccac ccacgtcgtg gacgtcaccg acgacaatgc ggtggcggcc ctgcccgctg cggtgctcga cgcgcacggg caggtcgacg gtctggtcaa catcgccggg atcgcgcaac ggttcgcgtt gttctccgac ctcggcgccg aggccctcga ccgggtgccg gtggtgaact tcaccggcac cgtgcggatg tgccgggcgt tcctgccgat cctgctggcg cgtccggagg ccaacatcac caacatgtcg agcctgtcgg ctctggtgcc gttcgccagt cagctgctct acagcgccag caaggccgcg gtgaaacagt tcagcgaagg cctggacgcc gaactcgtcg gtaccgacgt ccgtgtcgtg accgtgttcc cgggcaacgt cgccaccgaa ctcgcgaaga actccggggt ggagatgctc gacgccggca gccgacgggt gtacgccacc agccccgagg cggccggcag gaagatcgtc gccggtatcg cgagagaccg gtaccgggtg atcatcggcg cggacgccca tgtgttgcat gcccttgcgc ggatcgcgcc gcggcgcacc gcacggctgg tggccagaca gatcacatcc gttctccggg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccatcccgc tgtgccccgc gttgcgcacc gccatcgaga cggcggtggt gcaggggttg ctcccggtgc tggacgactc cgcgccgccg accaccgaag gggagccgtc ccgtgcgcat caccgatagg gtgttcgtcg tcaccggttc cggcaacggc atggggcggg aggtcgccgt ggagctgggc cggcgcgggg cacatgtcgc cgccgtcgac atcgatcggg aagggctcgc cggcacggcg gaactggttc gggccaccgg agcgcggatc accacccacg tcgtggacgt caccgacgac aatgcggtgg cggccctgcc cgctgcggtg ctcgacgcgc acgggcaggt cgacggtctg gtcaacatcg ccgggatcgc gcaacggttc gcgttgttct ccgacctcgg cgccgaggcc ctcgaccggg tgccggtggt gaacttcacc ggcaccgtgc ggatgtgccg ggcgttcctg ccgatcctgc tggcgcgtcc ggaggccaac atcaccaaca tgtcgagcct gtcggctctg gtgccgttcg ccagtcagct gctctacagc gccagcaagg ccgcggtgaa acagttcagc gaaggcctgg acgccgaact cgtcggtacc gacgtccgtg tcgtgaccgt gttcccgggc aacgtcgcca ccgaactcgc gaagaactcc ggggtggaga tgctcgacgc cggcagccga cgggtgtacg ccaccagccc cgaggcggcc ggcaggaaga tcgtcgccgg tatcgcgagn gaccggtacc gggtgatcat cggcgcggac gcccatgtgt tgcatgccct tgcgcggatc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcacggc tggtggccag acagatcaca tccgttctcc gggagcagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa