SSGCID: Target Page

MythA.00762.b

Short chain dehydrogenase (A0R4Z8 homolog)

CENTER ID:	MythA.00762.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00762.b.A1.GE28756	Full length( MythA.00762.b )		1	200

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1507
RefSeq:	WP_003925030.1
UniProt:	G7CEW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGRVHSAVCD VTSTEQVDAL IATAAEQMGR LDVLVNNAGL GGSTPVIDMT DEEWDRVLDV TLTSVMRATR AALRYFRDAG HGGVIVNNAS VLGWRAQHSQ SHYAAAKAGV MALTRCSAIE AVEYGVRINA VSPSIARHKF LEKTSSADLL XXXXXXXXXX XXAFGRAAEP WEVAATIAFL ASDYSSYLTG EVISVSSQHP

NT Sequence

CTGGGCCGGG TGCACAGCGC GGTGTGCGAT GTGACGTCGA CGGAGCAGGT CGACGCGCTG ATCGCCACCG CGGCCGAGCA GATGGGCCGG CTCGACGTCC TGGTGAACAA CGCCGGGCTG GGCGGCTCGA CGCCGGTCAT CGACATGACC GACGAGGAGT GGGACCGGGT CCTCGACGTC ACCCTCACCT CGGTGATGCG GGCGACCCGG GCCGCGCTGC GGTATTTCCG CGATGCCGGT CACGGCGGGG TGATCGTCAA CAACGCCAGT GTGCTGGGGT GGCGGGCGCA GCACTCGCAG TCGCACTACG CCGCCGCCAA GGCCGGTGTG ATGGCGCTGA CCCGGTGCAG CGCGATCGAG GCGGTCGAGT ACGGGGTGCG GATCAACGCG GTCTCGCCCA GCATCGCGCG GCACAAGTTC CTGGAGAAGA CCAGCTCGGC GGATCTGCTN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNAGGCGT TCGGCCGTGC CGCCGAACCG TGGGAGGTCG CGGCCACCAT CGCGTTCCTG GCCAGCGATT ACTCCAGTTA CCTGACCGGC GAAGTCATCT CCGTGTCGAG CCAGCACCCA

Details for MythA.00762.b.A1.GE28756

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	23kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLGRVHSAV CDVTSTEQVD ALIATAAEQM GRLDVLVNNA GLGGSTPVID MTDEEWDRVL DVTLTSVMRA TRAALRYFRD AGHGGVIVNN ASVLGWRAQH SQSHYAAAKA GVMALTRCSA IEAVEYGVRI NAVSPSIARH KFLEKTSSAD LLDRLADQEA FGRAAEPWEV AATIAFLASD YSSYLTGEVI SVS

Validated NT Sequence

ctcgacacgg agatgacttc gccggtcagg taactggagt aatcgctggc caggaacgcg atggtggccg cgacctccca cggttcggcg gcacggccga acgcctcctg gtcggccagc cggtccagca gatccgccga gctggtcttc tccaggaact tgtgccgcgc gatgctgggc gagaccgcgt tgatccgcac cccgtactcg accgcctcga tcgcgctgca ccgggtcagc gccatcacac cggccttggc ggcggcgtag tgcgactgcg agtgctgcgc ccgccacccc agcacactgg cgttgttgac gatcaccccg ccgtgaccgg catcgcggaa ataccgcagc gcggcccggg tcgcccgcat caccgaggtg agggtgacgt cgaggacccg gtcccactcc tcgtcggtca tgtcgatgac cggcgtcgag ccgcccagcc cggcgttgtt caccaggacg tcgagccggc ccatctgctc ggccgcggtg gcgatcagcg cgtcgacctg ctccgtcgac gtcacatcgc acaccgcgct gtgcacccgg cccagcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgggccgg gtgcacagcg cggtgtgcga tgtgacgtcg acggagcagg tcgacgcgct gatcgccacc gcggccgagc agatgggccg gctcgacgtc ctggtgaaca acgccgggct gggcggctcg acgccggtca tcgacatgac cgacgaggag tgggaccggg tcctcgacgt caccctcacc tcggtgatgc gggcgacccg ggccgcgctg cggtatttcc gcgatgccgg tcacggcggg gtgatcgtca acaacgccag tgtgctgggg tggcgggcgc agcactcgca gtcgcactac gccgccgcca aggccggtgt gatggcgctg acccggtgca gcgcgatcga ggcggtcgag tacggggtgc ggatcaacgc ggtctcgccc agcatcgcgc ggcacaagtt cctggagaag accagctcgg cggatctgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggcg ttcggccgtg ccgccgaacc gtgggaggtc gcggccacca tcgcgttcct ggccagcgat tactccagtt acctgaccgg cgaagtcatc tccgtgtcga gccagcaccc aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa