MythA.00750.c

Phosphate import ATP-binding protein pstB (A0R4C0 homolog)

CENTER ID: MythA.00750.c
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00750.c.A2.GE29587 potential soluble domain 83 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.863
RefSeq: WP_003924342.1
UniProt: G7CD21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGTNPVLRTV SVGVDLRPGA AVHRGDRVLP GRAGRTRSAA VPAASSRPGP GGAGEACAGG QSGGRGMTTA AREPVAGGNA GMRAEYLEVR GLTVDFDGFK AVSGVDLTLF QGDLRFLIGP NGAGKTTVID AIXXXXXXXX XXXXXVNKSG VELLGKKVHQ IARLGVGRTF QTASVFETLT VLQNLDIAAG AGRSWRTLLR RRGAVLPQIT ETLQTIGLAD LADEPAGTLA HGQKQWLEIG MLLVQNADVL LLDEPVAGMS HEEREQTGNL LRRIGGERTV VVVEHDMDFM RAFATXXXXX XXXXXXAGDR RGHRRGGAGQ PEGAGGLPRH RRRTARGGAL MLELVDXXXX XXXXXXXXXX XXXXREGPPD GVAAVMGHNG AGKTTLLRAA VGLLKCTSGR VFFDGADVTR LRPSARVXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX GARRPTADR
NT Sequence
ATGGGCACAA ACCCTGTTCT CCGAACGGTT TCCGTCGGAG TGGACCTACG CCCAGGGGCT GCTGTTCATC GTGGTGATCG GGTTCTTCCC GGCCGGGCTG GCCGGACTCG GAGCGCTGCG GTCCCGGCTG CGTCGTCGCG TCCCGGACCC GGCGGTGCCG GTGAAGCATG CGCAGGAGGT CAAAGTGGAG GCCGCGGTAT GACCACGGCG GCNCGCGAAC CGGTCGCCGG CGGCAACGCC GGGATGCGTG CCGAATACCT CGAGGTCCGC GGCCTGACGG TGGATTTCGA CGGGTTCAAG GCGGTCAGCG GTGTCGACCT CACCCTGTTC CAGGGTGACC TGCGGTTCCT GATCGGCCCC AACGGCGCCG GCAAGACCAC GGTGATCGAC GCGATCANNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGTGAA CAAGTCCGGG GTCGAACTGC TCGGCAAGAA GGTGCATCAG ATCGCCCGGC TCGGCGTGGG CCGAACCTTC CAGACCGCCA GCGTGTTCGA GACGCTGACC GTGCTGCAGA ACCTCGACAT CGCCGCGGGA GCCGGCCGCT CGTGGCGGAC CCTGCTGCGC CGCCGCGGCG CCGTGCTGCC GCAGATCACC GAGACGTTGC AGACCATCGG TCTGGCCGAC CTGGCGGACG AGCCGGCCGG CACCCTGGCC CACGGGCAGA AGCAGTGGCT GGAGATCGGC ATGCTGCTGG TGCAGAACGC CGACGTGCTG CTGTTGGACG AGCCGGTCGC CGGGATGAGC CACGAGGAGC GGGAGCAGAC CGGAAACCTG TTGCGCCGCA TCGGTGGTGA GCGCACCGTC GTCGTCGTCG AACACGATAT GGACTTCATG CGCGCGTTCG CCACCTNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGC GGGGGATCGC CGAGGGCACC GTCGAGGAGG TGCAGGCCAA CCCGAAGGTG CAGGAGGTCT ACCTCGGCAC CGCCGCCGAA CTGCCCGAGG AGGCGCACTG ATGCTCGAAC TCGTCGATNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GCCGCGAGGG GCCGCCCGAC GGTGTGGCGG CGGTGATGGG GCACAACGGC GCCGGGAAGA CCACCCTGCT GCGGGCCGCG GTCGGCCTGC TCAAGTGCAC GTCGGGCCGG GTGTTCTTCG ACGGGGCGGA CGTCACCCGG CTGCGCCCCA GCGCCCGGGT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGGCGCGAC GGCCGACGGC TGATCGA
Details for MythA.00750.c.A2.GE29587
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 38kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 72
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAEYLEVR GLTVDFDGFK AVSGVDLTLF QGDLRFLIGP NGAGKTTVID AITGLVPATG SVNKSGVELL GKKVHQIARL GVGRTFQTAS VFETLTVLQN LDIAAGAGRS WRTLLRRRGA VLPQITETLQ TIGLADLADE PAGTLAHGQK QWLEIGMLLV QNADVLLLDE PVAGMSHEER EQTGNLLRRI GGERTVVVVE HDMDFMRAFA TSXTXLARGQ XIAXGTVEXX XANPXXAXXL XXXXXRXXRX XXTDAXXXDV RXXXXXXXPX RXXXXXXXXX XXXGXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgtgc cgaatacctc gaggtccgcg gcctgacggt ggatttcgac gggttcaagg cggtcagcgg tgtcgacctc accctgttcc agggtgacct gcggttcctg atcggcccca acggcgccgg caagaccacg gtgatcgacg cgatcaccgg gctggtgccg gccaccgggt cggtgaacaa gtccggggtc gaactgctcg gcaagaaggt gcatcagatc gcccggctcg gcgtgggccg aaccttccag accgccagcg tgttcgagac gctgaccgtg ctgcagaacc tcgacatcgc cgcgggagcc ggccgctcgt ggcggaccct gctgcgccgc cgcggcgccg tgctgccgca gatcaccgag acgttgcaga ccatcggtct ggccgacctg gcggacgagc cggccggcac cctggcccac gggcagaagc agtggctgga gatcggcatg ctgctggtgc agaacgccga cgtgctgctg ttggacgagc cggtcgccgg gatgagccac gaggagcggg agcagaccgg aaacctgttg cgccgcatcg gtggtgagcg caccgtcgtc gtcgtcgaac acgatatgga cttcatgcgc gcgttcgcca cctcggngac gnngctggcc cgcgggcagg ngatcgccga nggcaccgtc gagnagnngc nggccaaccc naangnngcn gnangnctac ntcngcnncn cnnccgaant gnncgagnan gnnncactga tgctnnantc nncgatgtgc gannnngann nnnnnnnnnn gntccnnnnc gtcancnnnn angnnnnnnc nganngngnc nnngnnangg nnnnnnnnnn nngnnnnacn ccnnntgnnn nnntnnnnnn nnntnnnnnn nnnnnnanng nngnnnnnnc cannnnnnnn nannnnnnnn nnnnnnnnnn nnnnnnnnnn naangnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAEYLEVR GLTVDFDGFK AVSGVDLTLF QGDLRFLIGP NGAGKTTVID AIXXXXXXXX XXXXXVNKSG VELLGKKVHQ IARLGVGRTF QTASVFETLT VLQNLDIAAG AGRSWRTLLR RRGAVLPQIT ETLQTIGLAD LADEPAGTLA HGQKQWLEIG MLLVQNADVL LLDEPVAGMS HEEREQTGNL LRRIGGERTV VVVEHDMDFM RAFATXXXXX XXXXXXAGDR RGHRRGGAGQ PEGAGGLPRH RRRTARGGAL MLELVDXXXX XXXXXXXXXX XXXXREGPPD GVAAVMGHNG AGKTTLLRAA VGLLKCTSGR VFFDGADVTR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgccgaa tacctcgagg tccgcggcct gacggtggat ttcgacgggt tcaaggcggt cagcggtgtc gacctcaccc tgttccaggg tgacctgcgg ttcctgatcg gccccaacgg cgccggcaag accacggtga tcgacgcgat cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaacaagt ccggggtcga actgctcggc aagaaggtgc atcagatcgc ccggctcggc gtgggccgaa ccttccagac cgccagcgtg ttcgagacgc tgaccgtgct gcagaacctc gacatcgccg cgggagccgg ccgctcgtgg cggaccctgc tgcgccgccg cggcgccgtg ctgccgcaga tcaccgagac gttgcagacc atcggtctgg ccgacctggc ggacgagccg gccggcaccc tggcccacgg gcagaagcag tggctggaga tcggcatgct gctggtgcag aacgccgacg tgctgctgtt ggacgagccg gtcgccggga tgagccacga ggagcgggag cagaccggaa acctgttgcg ccgcatcggt ggtgagcgca ccgtcgtcgt cgtcgaacac gatatggact tcatgcgcgc gttcgccacc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcggggg atcgccgagg gcaccgtcga ggaggtgcag gccaacccga aggtgcagga ggtctacctc ggcaccgccg ccgaactgcc cgaggaggcg cactgatgct cgaactcgtc gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccgc gaggggccgc ccgacggtgt ggcggcggtg atggggcaca acggcgccgg gaagaccacc ctgctgcggg ccgcggtcgg cctgctcaag tgcacgtcgg gccgggtgtt cttcgacggg gcggacgtca cccggaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa