SSGCID: Target Page

MythA.00750.b

Phosphate import ATP-binding protein pstB (A0R4C0 homolog)

CENTER ID:	MythA.00750.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00750.b.A1.GE28752	Full length( MythA.00750.b )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2683
RefSeq:	WP_003926211.1
UniProt:	G7CGI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKRLDLKDV NIYYGGFHAV ADINLSVPPR MVTAFIGPSG CGKSTVLRTL NRMHEVIPGA RVEGSVLLDG EDIYAPGVDP VSVRKTIGMV FQRPNPFPTM SIRDNVVAGL KLQGVRNKKV LDEVCERSLR GANLWNEVKD RLDKPGGGLS GGQQQRLCIA RAIAVEPDVL LMDEPCSALD PISTLAIEDL IAKLKQDFTI VIVTHNMQQA ARVSDQTAFF NLEATGRPGR LIEIDDTEKI FSNPSQKATE DYISGRFG

NT Sequence

ATGGCCAAAC GGCTCGATCT GAAAGACGTC AACATCTACT ATGGCGGATT CCACGCCGTC GCCGACATCA ACCTGTCCGT GCCGCCGCGG ATGGTGACCG CGTTCATCGG TCCGTCCGGG TGCGGCAAGT CGACGGTGCT GCGCACCCTC AACCGGATGC ACGAGGTCAT CCCGGGCGCC CGGGTGGAGG GTTCGGTACT GCTCGACGGT GAGGACATCT ACGCACCCGG TGTCGACCCG GTCAGCGTCC GCAAGACCAT CGGCATGGTG TTCCAGCGGC CGAATCCATT TCCCACCATG TCGATTCGCG ACAACGTGGT GGCCGGGCTG AAGCTGCAGG GCGTGCGGAA CAAGAAGGTG CTCGACGAGG TGTGCGAACG CTCACTGCGG GGCGCCAACC TGTGGAACGA GGTCAAGGAC CGGCTGGACA AGCCGGGTGG CGGGCTGTCC GGCGGTCAGC AGCAGCGGCT GTGCATCGCC CGGGCGATCG CGGTGGAACC GGATGTGCTG CTGATGGACG AACCGTGCTC GGCGCTCGAC CCGATCTCCA CGCTGGCCAT CGAGGATCTG ATCGCCAAGC TCAAGCAGGA CTTCACGATC GTGATCGTCA CCCACAACAT GCAGCAGGCC GCCCGGGTCA GCGATCAGAC GGCCTTCTTC AACCTGGAGG CGACCGGACG GCCGGGCCGG CTCATCGAGA TCGACGACAC CGAGAAGATC TTCTCCAACC CCAGCCAGAA GGCCACCGAG GATTACATCT CCGGCCGGTT CGGC

Details for MythA.00750.b.A1.GE28752

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	30kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKRLDLKD VNIYYGGFHA VADINLSVPP RMVTAFIGPS GCGKSTVLRT LNRMHEVIPG ARVEGSVLLD GEDIYAPGVD PVSVRKTIGM VFQRPNPFPT MSIRDNVVAG LKLQGVRNKK VLDEVCERSL RGANLWNEVK DRLDKPGGGL SGGQQQRLCI ARAIAVEPDV LLMDEPCSAL DPISTLAIED LIAKLKQDFT IVIVTHNMQQ AARVSDQTAF FNLEATGRPG RLIEIDDTEK IFSNPSQKAT EDYISGRFG

Validated NT Sequence

ttattagccg aaccggccgg agatgtaatc ctcggtggcc ttctggctgg ggttggagaa gatcttctcg gtgtcgtcga tctcgatgag ccggcccggc cgtccggtcg cctccaggtt gaagaaggcc gtctgatcgc tgacccgggc ggcctgctgc atgttgtggg tgacgatcac gatcgtgaag tcctgcttga gcttggcgat cagatcctcg atggccagcg tggagatcgg gtcgagcgcc gagcacggtt cgtccatcag cagcacatcc ggttccaccg cgatcgcccg ggcgatgcac agccgctgct gctgaccgcc ggacagcccg ccacccggct tgtccagccg gtccttgacc tcgttccaca ggttggcgcc ccgcagtgag cgttcgcaca cctcgtcgag caccttcttg ttccgcacgc cctgcagctt cagcccggcc accacgttgt cgcgaatcga catggtggga aatggattcg gccgctggaa caccatgccg atggtcttgc ggacgctgac cgggtcgaca ccgggtgcgt agatgtcctc accgtcgagc agtaccgaac cctccacccg ggcgcccggg atgacctcgt gcatccggtt gagggtgcgc agcaccgtcg acttgccgca cccggacgga ccgatgaacg cggtcaccat ccgcggcggc acggacaggt tgatgtcggc gacggcgtgg aatccgccat agtagatgtt gacgtctttc agatcgagcc gtttggccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccaaacgg ctcgatctga aagacgtcaa catctactat ggcggattcc acgccgtcgc cgacatcaac ctgtccgtgc cgccgcggat ggtgaccgcg ttcatcggtc cgtccgggtg cggcaagtcg acggtgctgc gcaccctcaa ccggatgcac gaggtcatcc cgggcgcccg ggtggagggt tcggtactgc tcgacggtga ggacatctac gcacccggtg tcgacccggt cagcgtccgc aagaccatcg gcatggtgtt ccagcggccg aatccatttc ccaccatgtc gattcgcgac aacgtggtgg ccgggctgaa gctgcagggc gtgcggaaca agaaggtgct cgacgaggtg tgcgaacgct cactgcgggg cgccaacctg tggaacgagg tcaaggaccg gctggacaag ccgggtggcg ggctgtccgg cggtcagcag cagcggctgt gcatcgcccg ggcgatcgcg gtggaaccgg atgtgctgct gatggacgaa ccgtgctcgg cgctcgaccc gatctccacg ctggccatcg aggatctgat cgccaagctc aagcaggact tcacgatcgt gatcgtcacc cacaacatgc agcaggccgc ccgggtcagc gatcagacgg ccttcttcaa cctggaggcg accggacggc cgggccggct catcgagatc gacgacaccg agaagatctt ctccaacccc agccagaagg ccaccgagga ttacatctcc ggccggttcg gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa