SSGCID: Target Page

MythA.00750.a

Phosphate import ATP-binding protein pstB (A0R4C0 homolog)

CENTER ID:	MythA.00750.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00750.a.A1.GE28751	Full length( MythA.00750.a )		1	245

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2084
RefSeq:	WP_003925608.1
UniProt:	G7CIL2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQPKAVSLA ATDIHLAFGP NKVLRGVDLE VPAGTTAAVI GPSGSGKSTL LRTLNRLYEP DRGDILLDGR SVLRDNPDQL RQRIGMVFQH FNLFPHRTVL DNVTLAPRKL KRLSRDEARE LGLRQLERVG LRHKAEVRPT TLSGGQQQRV AIARALAMDP QVMFFDEATS ALDPELVKGI LALIADLAES GMTMVVVTHE MGFARSTADT VVFMDHGQVV ESGPPQQIFE SAATERLRRF LSQVL

NT Sequence

ATGACCCAAC CGAAAGCGGT GTCGTTGGCG GCCACCGACA TTCACCTGGC GTTCGGGCCC AACAAGGTGC TGCGCGGCGT CGACCTCGAG GTCCCAGCCG GCACCACCGC CGCCGTGATC GGTCCGTCCG GGTCGGGCAA GTCGACCCTG TTGCGCACCC TGAACCGGCT CTACGAACCG GACCGCGGTG ACATCCTGCT CGACGGCCGG TCGGTGCTCA GGGACAACCC GGATCAGTTG CGGCAACGCA TCGGCATGGT GTTTCAGCAC TTCAACCTGT TCCCGCACCG CACCGTGCTG GACAACGTGA CCCTGGCACC GCGCAAACTC AAGAGACTCA GCCGCGACGA GGCCCGCGAA CTCGGTCTGC GACAACTGGA AAGGGTGGGT CTGCGGCACA AGGCCGAGGT GCGGCCCACC ACCCTGTCCG GTGGGCAGCA GCAGCGGGTG GCGATCGCCC GGGCGTTGGC GATGGACCCG CAGGTGATGT TCTTCGACGA GGCCACCTCG GCGCTGGACC CGGAACTGGT CAAGGGCATC CTGGCGTTGA TCGCCGACCT GGCCGAGAGC GGGATGACCA TGGTGGTGGT GACCCACGAG ATGGGCTTCG CCCGGTCCAC CGCCGACACC GTGGTGTTCA TGGACCACGG TCAGGTGGTG GAATCCGGCC CGCCACAGCA GATCTTCGAA TCCGCGGCCA CCGAGAGGCT GCGCCGTTTC TTGTCCCAGG TGTTG

Details for MythA.00750.a.A1.GE28751

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMTQPKAV SLAATDIHLA FGPNKVLRGV DLEVPAGTTA AVIGPSGSGK STLLRTLNRL YEPDRGDILL DGRSVLRDNP DQLRQRIGMV FQHFNLFPHR TVLDNVTLAP RKLKRLSRDE ARELGLRQLE RVGLRHKAEV RPTTLSGGQQ QRVAIARALA MDPQVMFFDE ATSALDPELV KGILALIADL AESGMTMVVV THEMGFARST ADTVVFMDHG QVVESGPPQQ IFESAATERL RRFLSXXL

Validated NT Sequence

catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacccaa ccgaaagcgg tgtcgttggc ggccaccgac attcacctgg cgttcgggcc caacaaggtg ctgcgcggcg tcgacctcga ggtcccagcc ggcaccaccg ccgccgtgat cggtccgtcc gggtcgggca agtcgaccct gttgcgcacc ctgaaccggc tctacgaacc ggaccgcggt gacatcctgc tcgacggccg gtcggtgctc agggacaacc cggatcagtt gcggcaacgc atcggcatgg tgtttcagca cttcaacctg ttcccgcacc gcaccgtgct ggacaacgtg accctggcac cgcgcaaact caagagactc agccgcgacg aggcccgcga actcggtctg cgacaactgg aaagggtggg tctgcggcac aaggccgagg tgcggcccac caccctgtcc ggtgggcagc agcagcgggt ggcgatcgcc cgggcgttgg cgatggaccc gcaggtgatg ttcttcgacg aggccacctc ggcgctggac ccggaactgg tcaagggcat cctggcgttg atcgccgacc tggccgagag cgggatgacc atggtggtgg tgacccacga gatgggcttc gcccggtcca ccgccgacac cgtggtgttc atggaccacg gtcaggtggt ggaatccggc ccgccacagc agatcttcga atccgcggcc accgagaggc tgcgccgttt cttgtcccng ntgttgtaat aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTQPKAVSL AATDIHLAFG PNKVLRGVDL EVPAGTTAAV IGPSGSGKST LLRTLNRLYE PDRGDILLDG RSVLRDNPDQ LRQRIGMVFQ HFNLFPHRTV LDNVTLAPRK LKRLSRDEAR ELGLRQLERV GLRHKAEVRP TTLSGGQQQR VAIARALAMD PQVMFFDEAT SALDPELVKG ILALIADLAE SGMTMVVVTH EMGFARSTAD TVVFMDHGQV VESGPPQQIF ESAATERLRR FLSQVL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccaaccg aaagcggtgt cgttggcggc caccgacatt cacctggcgt tcgggcccaa caaggtgctg cgcggcgtcg acctcgaggt cccagccggc accaccgccg ccgtgatcgg tccgtccggg tcgggcaagt cgaccctgtt gcgcaccctg aaccggctct acgaaccgga ccgcggtgac atcctgctcg acggccggtc ggtgctcagg gacaacccgg atcagttgcg gcaacgcatc ggcatggtgt ttcagcactt caacctgttc ccgcaccgca ccgtgctgga caacgtgacc ctggcaccgc gcaaactcaa gagactcagc cgcgacgagg cccgcgaact cggtctgcga caactggaaa gggtgggtct gcggcacaag gccgaggtgc ggcccaccac cctgtccggt gggcagcagc agcgggtggc gatcgcccgg gcgttggcga tggacccgca ggtgatgttc ttcgacgagg ccacctcggc gctggacccg gaactggtca agggcatcct ggcgttgatc gccgacctgg ccgagagcgg gatgaccatg gtggtggtga cccacgagat gggcttcgcc cggtccaccg ccgacaccgt ggtgttcatg gaccacggtc aggtggtgga atccggcccg ccacagcaga tcttcgaatc cgcggccacc gagaggctgc gccgtttctt gtcccaggtg ttgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa