MythA.00738.b

RNA polymerase sigma factor SigA

CENTER ID: MythA.00738.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00738.b.B1.GE38638 full length 1 504
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3244
UniProt: G7CI54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYVAATEASP ATDEPKRTAT KTPAKKAAAT KSASGTTKSG NGSAPAKRAA KSSRTKSESG SATKSRATKA TKATKSTATK ATKSAAKAAG KTTRAKSGAA NTRARTKKDA DETLVDDVDA VDIEPDVAED IDEADLDLTD IDVDDEDDAT EATAEDDDDE DDDEIDEPSE KDKASGDFVW DEEESEALRQ ARKDAELTAS ADSVRAYLKQ IGKVALLNAE EEVDLAKRIE AGLFAAQKLA EYAEKGEKIP TQLRRDMQWI CRDGERAKNH LLEANLRLVV SLAKRYTGRG MAFLDLIQEG NLGLIRAVEK FDYTKGYKFS TYATWWIRQA ITRAMADQAR TIRIPVHMVE VINKLGRIQR ELLQDLGREP TPEELAKEMD ITPEKVLEIQ QYAREPISLD QTIGDEGDSQ LGDFIEDSEA VVAVDAVSFT LLQDQLQSVL ETLSEREAGV VRLRFGLTDG QPRTLDEIGQ VYGVTRERIR QIESKTMSKL RHPSRSQVLR DYLD
NT Sequence
gtgtacgtgg cagcgacaga ggcaagcccg gcaacagacg agccgaagcg caccgctacc aagactcccg cgaagaaggc agccgcaacc aagagtgcga gcggcacgac caagagtggg aacgggtcgg cgccggccaa gcgggccgcc aagtccagcc gaaccaagag cgagtcgggc tcggcgacca agagccgggc caccaaagcc acgaaggcca ccaaatcgac ggccaccaag gcgaccaagt ccgcggccaa ggcggccggt aagaccactc gcgccaagtc cggcgctgcg aacacccgtg cccgcaccaa gaaggacgcc gacgagaccc tggtcgacga cgtcgacgcg gtcgacatcg aacccgacgt agccgaggac atcgacgagg ccgacctgga cctgaccgac atcgacgtcg acgacgagga cgacgccacc gaggcgaccg ccgaggatga cgacgacgag gacgacgacg agatcgacga accgtcggag aaggacaagg cgtcgggcga tttcgtctgg gacgaggagg agtccgaggc gttgcggcag gcccgcaagg acgccgagct gacggcttcc gcggactcgg tgcgcgccta cctcaagcag atcggcaagg tcgcgctgct caacgccgag gaggaggtcg acctcgccaa gcggatcgag gccgggctgt tcgccgccca gaagctggcc gagtacgccg agaagggcga gaagatcccg acccagctgc gtcgcgacat gcagtggatc tgccgcgacg gcgagcgcgc gaaaaaccat ctgctggagg cgaacctgcg cctggtggtg tcgctggcca agcgctacac cggacggggc atggcgttcc tggacctcat ccaggagggc aacctgggcc tgatccgcgc ggtggagaag ttcgactaca ccaagggcta caagttctcc acctacgcca cctggtggat ccgccaggcc atcacccgcg ccatggccga ccaggcccgc accatccgga tcccggtgca catggtcgag gtcatcaaca agctcggccg catccagcgt gagctgctgc aggacctcgg ccgcgagccc acccccgagg agctcgccaa ggagatggac atcacgccgg agaaggtgct ggagatccag cagtacgcgc gggagcccat ctcactggac cagaccatcg gcgacgaggg cgacagccaa ctgggcgatt tcatcgagga ctccgaggcc gtggtggccg tcgacgcggt gagcttcacg ctgctgcagg atcagctgca gtcggtgctc gagacgctct cggaacgtga ggccggggtg gttcggttgc gcttcgggct gaccgacggc cagccgcgca ccctcgatga gatcggccag gtgtacggcg tcacccgcga acgcatccgc cagatcgagt cgaagaccat gagcaagctg cgccacccga gccgttctca ggtcctgcgc gactatctgg actga
Details for MythA.00738.b.B1.GE38638
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 57kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMY VAATEASPAT DEPKRTATKT PAKKAAATKS ASGTTKSGNG SAPAKRAAKS SRTKSESGSA TKSRATKATK ATKSTATKAT KSAAKAAGKT TRAKSGAANT RARTKKDADE TLVDDVDAVD IEPDVAEDID EADLDLTDID VDDEDDATEA TAEDDDDEDD DEIDEPSEKD KASGDFVWDE EESEALRQAR KDAELTASAD SVRAYLKQIG KVALLNAEEE VDLAKRIEAG LFAAQKLAEY AEKGEKIPTQ LRRDMQWICR DGERAKNHLL EANLRLVVSL AKRYTGRGMA FLDLIQEGNL GLIRAVEKFD YTKGYKFSTY ATWWIRQAIT RAMADQARTI RIPVHMVEVI NKLGRIQREL LQDLGREPTP EELAKEMDIT PEKVLEIQQY AREPISLDQT IGDEGDSQLG DFIEDSEAVV AVDAVSFTLL QDQLQSVLET LSEREAGVVR LRFGLTDGQP RTLDEIGQVY GVTRERIRQI ESKTMSKLRH PSRSQVLRDY LD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtacg tggcagcgac agaggcaagc ccggcaacag acgagccgaa gcgcaccgct accaagactc ccgcgaagaa ggcagccgca accaagagtg cgagcggcac gaccaagagt gggaacgggt cggcgccggc caagcgggcc gccaagtcca gccgaaccaa gagcgagtcg ggctcggcga ccaagagccg ggccaccaaa gccacgaagg ccaccaaatc gacggccacc aaggcgacca agtccgcggc caaggcggcc ggtaagacca ctcgcgccaa gtccggcgct gcgaacaccc gtgcccgcac caagaaggac gccgacgaga ccctggtcga cgacgtcgac gcggtcgaca tcgaacccga cgtagccgag gacatcgacg aggccgacct ggacctgacc gacatcgacg tcgacgacga ggacgacgcc accgaggcga ccgccgagga tgacgacgac gaggacgacg acgagatcga cgaaccgtcg gagaaggaca aggcgtcggg cgatttcgtc tgggacgagg aggagtccga ggcgttgcgg caggcccgca aggacgccga gctgacggct tccgcggact cggtgcgcgc ctacctcaag cagatcggca aggtcgcgct gctcaacgcc gaggaggagg tcgacctcgc caagcggatc gaggccgggc tgttcgccgc ccagaagctg gccgagtacg ccgagaaggg cgagaagatc ccgacccagc tgcgtcgcga catgcagtgg atctgccgcg acggcgagcg cgcgaaaaac catctgctgg aggcgaacct gcgcctggtg gtgtcgctgg ccaagcgcta caccggacgg ggcatggcgt tcctggacct catccaggag ggcaacctgg gcctgatccg cgcggtggag aagttcgact acaccaaggg ctacaagttc tccacctacg ccacctggtg gatccgccag gccatcaccc gcgccatggc cgaccaggcc cgcaccatcc ggatcccggt gcacatggtc gaggtcatca acaagctcgg ccgcatccag cgtgagctgc tgcaggacct cggccgcgag cccacccccg aggagctcgc caaggagatg gacatcacgc cggagaaggt gctggagatc cagcagtacg cgcgggagcc catctcactg gaccagacca tcggcgacga gggcgacagc caactgggcg atttcatcga ggactccgag gccgtggtgg ccgtcgacgc ggtgagcttc acgctgctgc aggatcagct gcagtcggtg ctcgagacgc tctcggaacg tgaggccggg gtggttcggt tgcgcttcgg gctgaccgac ggccagccgc gcaccctcga tgagatcggc caggtgtacg gcgtcacccg cgaacgcatc cgccagatcg agtcgaagac catgagcaag ctgcgccacc cgagccgttc tcaggtcctg cgcgactatc tggactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat