MythA.00654.a

Putative acyl-CoA dehydrogenase (A0R2Q7 homolog)

CENTER ID: MythA.00654.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00654.a.A2.GE29586 potential soluble domain 21 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.2541
RefSeq: WP_003926067.1
UniProt: G7CKZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQNRSKPDGE VHMTTSVIVA GARTPVGKLM GSLKDFAATD LGAIAIKGAL EKAKVPASAV EYVIMGQVLT AGAGQMPARQ SAVXXXXXXX XXXXXXXXXX XQQDVSVRYR LDRPGRPADP RXXXXXXXXX XXXXXXXXXX XXXXXXXXXX SRAGYKYGDV TVLDHMAYDG LHDVFTDQPM GALTEQRNDI DKFTREQQDE FAVRSHQKAA AAWKDGVFAD EVVPVQIPQR KGDPIEFLED EGIRANTTLE SLAGLKPAFR KDGTITAGSA SQISDGAAAV VVMSKKKAQE MGLTWLCEIG AXXXGGGAGP DSSLQSQPAN AIKKAIAREG ITVDQLDIVE INEAFAAVAL ASTQELGVNP DIVNINGGAI AIGHPIGASG ARITLHAALE LSRRGSGYAV AALCGAGGQG DALVXXXXXX XXXXXG
NT Sequence
CTGCAGAACC GATCAAAACC CGATGGGGAG GTTCACATGA CGACGTCTGT GATCGTTGCT GGCGCCCGCA CGCCCGTGGG CAAGTTGATG GGTTCTCTCA AGGACTTCGC GGCCACCGAT CTCGGCGCGA TCGCCATCAA GGGCGCCCTG GAGAAGGCCA AGGTGCCGGC CTCCGCCGTC GAGTACGTGA TCATGGGCCA GGTGCTCACC GCGGGCGCCG GTCAGATGCC CGCCCGGCAG TCGGCGGTNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NATCAACAAG ATGTGTCTGT CCGGTATCGA CTCGATCGCC CTGGCCGACC AGCTGATCCG CGCCNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC AGCCGGGCCG GATACAAGTA CGGCGACGTC ACCGTGCTCG ACCACATGGC CTATGACGGT CTGCACGACG TGTTCACCGA TCAGCCGATG GGCGCGCTGA CCGAACAGCG CAACGACATC GACAAGTTCA CCCGCGAGCA GCAGGACGAG TTCGCGGTCC GGTCGCACCA GAAGGCCGCC GCGGCGTGGA AGGACGGCGT CTTCGCCGAC GAGGTCGTTC CGGTCCAGAT CCCGCAGCGC AAGGGCGACC CGATCGAGTT CCTCGAGGAC GAGGGCATCC GGGCCAACAC CACCCTCGAA TCGCTGGCCG GGCTCAAGCC GGCGTTCCGC AAAGACGGCA CCATCACCGC CGGTTCGGCG TCGCAGATCT CCGACGGGGC GGCCGCGGTC GTGGTGATGA GCAAGAAGAA GGCCCAGGAA ATGGGCTTGA CCTGGCTCTG TGAGATCGGC GCNNNNNNNN NNGGGGGGGG GGCCGGCCCC GACTCGTCGC TGCAGTCCCA GCCGGCGAAC GCGATCAAGA AGGCGATCGC CCGGGAGGGC ATCACCGTCG ACCAGCTCGA CATCGTGGAG ATCAACGAGG CGTTCGCCGC GGTGGCGCTC GCCTCCACCC AGGAACTCGG CGTCAACCCC GACATCGTCA ACATCAACGG CGGCGCCATC GCCATCGGCC ACCCGATCGG GGCGTCCGGT GCGCGTATCA CGCTGCACGC GGCGCTGGAG CTGTCCCGGC GTGGTTCGGG CTACGCGGTC GCGGCTCTGT GCGGCGCCGG TGGCCAGGGC GATGCGCTGG TGNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCGGGA
Details for MythA.00654.a.A2.GE29586
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 60
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGARTPVGK LMGSLKDFAA TDLGAIAIKG ALEKAKVPAS AVEYVIMGQV LTAGAGQMPA RQSAVAAGIP WDVPALSINK MCLSGIDSIA LADQLIRAGE FDVVVAGGQE SMSRAPHLLM DSRSGYKYGD VTVLDHMAYD GLHDVFTDQP MGALTEQRND IDKFTREQQD EFAVRSHQKA AAAWKDGVFA DEVVPVQIPQ RKGDPIEFLE DEGIRANTTL ESLXGLKPAF RKDGTITAGS ASQISDGAAA VVVMSKXXXX XMGLTWXCXI GAHGVXXXPR LVAAVPXGER XXEXXXRXXS XSXSSXSXXX XXXXXXXXXX XXXXXXXXNX XXXXXXXXGX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgggc gcccgcacgc ccgtgggcaa gttgatgggt tctctcaagg acttcgcggc caccgatctc ggcgcgatcg ccatcaaggg cgccctggag aaggccaagg tgccggcctc cgccgtcgag tacgtgatca tgggccaggt gctcaccgcg ggcgccggtc agatgcccgc ccggcagtcg gcggtggcgg ccggcatccc gtgggacgtg ccggcgctga gcatcaacaa gatgtgtctg tccggtatcg actcgatcgc cctggccgac cagctgatcc gcgccgggga gttcgacgtg gtggtcgccg gcggccagga gtcgatgagc agggcgccgc acctgctgat ggacagccgg tccggataca agtacggcga cgtcaccgtg ctcgaccaca tggcctatga cggtctgcac gacgtgttca ccgatcagcc gatgggcgcg ctgaccgaac agcgcaacga catcgacaag ttcacccgcg agcagcagga cgagttcgcg gtccggtcgc accagaaggc cgccgcggcg tggaaggacg gcgtcttcgc cgacgaggtc gttccggtcc agatcccgca gcgcaagggc gacccgatcg agttcctcga ggacgagggc atccgggcca acaccaccct cgaatcgctg gncgggctca agccggcgtt ccgcaaagac ggcaccatca ccgccggttc ggcgtcgcag atctccgacg gggcggccgc ggtcgtggtg atgagcaaga anaangnccn nnaaatgggc ttgacctggn tctgtganat cggcgcgcac ggngtgnngg ncnggccccg actcgtcgct gcagtcccag nnggngaacg cnatcangaa nnnnatcnnc gnanggnatc ancgtcganc agctcgnnnt cnngnnatna nnagngnncn nnggnggngn ncncnnncnn nnnnnnngnn tnanccnnan nnnnnaatna nngnnnnntn ncntnnnnnc nnnngggnnn cgnngnnnnn nnnnncnnnn nnngnnntgn nnnnnnnnnn nnnnnntnnn nnnnnnngnn nnnnnccnnn nnnnannna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGARTPVGK LMGSLKDFAA TDLGAIAIKG ALEKAKVPAS AVEYVIMGQV LTAGAGQMPA RQSAXXXXXX XXXXXXXXXX XXXQQDVSVR YRLDRPGRPA DPRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXSRAGYKYG DVTVLDHMAY DGLHDVFTDQ PMGALTEQRN DIDKFTREQQ DEFAVRSHQK AAAAWKDGVF ADEVVPVQIP QRKGDPIEFL EDEGIRANTT LESLAGLKPA FRKDGTITAG SASQISDGAA AVVVMSKKKA QEMGLTWLCE IGXXXXGGGA GPDSSLQSQP ANAIKKAIAR EGITVDQLDI VEINEAFAAV ALASTQELGV NPDIVNINGG AIAIGHPIGA SGARITLHAA LELSRRGSGY AVAALCGAGG QGDALV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcccgc acgcccgtgg gcaagttgat gggttctctc aaggacttcg cggccaccga tctcggcgcg atcgccatca agggcgccct ggagaaggcc aaggtgccgg cctccgccgt cgagtacgtg atcatgggcc aggtgctcac cgcgggcgcc ggtcagatgc ccgcccggca gtcggcggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcaacaa gatgtgtctg tccggtatcg actcgatcgc cctggccgac cagctgatcc gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagccgggcc ggatacaagt acggcgacgt caccgtgctc gaccacatgg cctatgacgg tctgcacgac gtgttcaccg atcagccgat gggcgcgctg accgaacagc gcaacgacat cgacaagttc acccgcgagc agcaggacga gttcgcggtc cggtcgcacc agaaggccgc cgcggcgtgg aaggacggcg tcttcgccga cgaggtcgtt ccggtccaga tcccgcagcg caagggcgac ccgatcgagt tcctcgagga cgagggcatc cgggccaaca ccaccctcga atcgctggcc gggctcaagc cggcgttccg caaagacggc accatcaccg ccggttcggc gtcgcagatc tccgacgggg cggccgcggt cgtggtgatg agcaagaaga aggcccagga aatgggcttg acctggctct gtgagatcgg cgcnnnnnnn nnnggggggg gggccggccc cgactcgtcg ctgcagtccc agccggcgaa cgcgatcaag aaggcgatcg cccgggaggg catcaccgtc gaccagctcg acatcgtgga gatcaacgag gcgttcgccg cggtggcgct cgcctccacc caggaactcg gcgtcaaccc cgacatcgtc aacatcaacg gcggcgccat cgccatcggc cacccgatcg gggcgtccgg tgcgcgtatc acgctgcacg cggcgctgga gctgtcccgg cgtggttcgg gctacgcggt cgcggctctg tgcggcgccg gtggccaggg cgatgcgctg gtgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa