MythA.00629.l

AMP-dependent synthetase and ligase

CENTER ID: MythA.00629.l
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.l.B2.GE40029 ordered domain 19 558
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.l.B2.PW37923 ordered domain 19 558
MythA.00629.l.B2.PW37995 ordered domain 19 558

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3334
RefSeq: WP_003926866.1
UniProt: G7CIS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTEFQPVES DLTSDFRTGF VPFPPDRVEE YRRAGYWTGR RLDSILSDAA AAQPDKAAVI DPDRSHTFAE LDALADRVAA GLADLGIAPG DRVLLQMPND TRFAVAFFGL LRAGAIPVMC LPGHRSAEMS HFAEVSGAVG LIIPERANGF DYRELARALT ASRPRLRHVI VDGATDPSGD GFVPWSALAE FAGPAVPRPP VDTTAPALLL VSGGTTGLPK LIPRTHDDYV YTAVASARAY EMTSDDVYLV VLPAAHNFPL ACPGMLGAMT VGASTVFTAD PSPEAAFPLI NRHRVTVTGL VNALAKLWAQ ACEWEPELPE SLRVVQVGGS RMSPQEAQFI LDRLTPGMSQ IFGMAEGMLN FTRPGDPIDV VVHTQGRPMS PHDEMRVVDE SGAEVAPGEE GELLVRGPNT LNGYYRDDEA NARSFSPDGF YRTGDRVRIF ADGPRAGHVE VTGRVKDVIH RGGETVSATD LEDHLLTHPA IYAAAAVPLP DEFLGEKICA AVVFRGAPVS LTELNGYLDE RGVATHARPD VLVALPSLPM TAVGKVDKKK VVEQVLSQ
NT Sequence
atgaccaccg agttccaacc cgtcgaaagc gatctgacca gcgacttccg caccggattc gtcccgttcc caccggaccg ggtcgaggag tatcgacgcg ccgggtactg gaccggacgt cggctggaca gcatcctttc cgacgcggcg gcggctcagc ccgacaaggc cgcggtgatc gacccggacc gcagccacac cttcgccgag ctggatgccc tcgccgaccg ggtcgccgcc ggactggccg atctcggcat cgcccccggt gaccgggtgc tgctgcagat gcccaacgac acccggttcg cggtcgcgtt cttcgggttg ctgcgggcgg gcgccatccc ggtgatgtgc ctgccgggcc accggtcggc cgagatgagc catttcgccg aggtgtccgg cgcggtgggg ctgatcattc cggagcgggc gaacggcttc gactaccgcg aactggccag agcgctcacc gcgagccgtc cacggttgcg ccatgtgatc gtcgacgggg ccaccgaccc gtcgggcgac gggttcgtgc cgtggtccgc actcgccgag ttcgccgggc cggccgtccc ccgcccgccg gtcgacacca ccgcgccggc cctgctgctg gtctcggggg gcaccaccgg cctgccgaag ctgatcccgc ggacccacga cgattacgtc tacaccgcgg tggccagcgc ccgggcctat gagatgacgt ccgacgacgt gtacctggtg gtgctgcctg ccgcccacaa cttcccgctg gcctgtccgg gcatgctggg cgcgatgacg gtcggggcgt ccaccgtgtt caccgccgac ccgagccccg aggcggcgtt cccgctgatc aaccggcatc gggtcacggt gaccgggctg gtcaacgcgc tggccaaact gtgggcgcag gcctgcgagt gggaaccgga actgcccgag tcactgcggg tggtgcaggt cggcggatca cggatgtccc cgcaggaggc ccagttcatc ctggaccggc tgaccccggg gatgtcgcag atcttcggga tggccgaggg catgctgaac ttcacccggc ccggtgaccc gatcgacgtc gtggtgcaca cccagggccg gccgatgtcg ccgcacgacg agatgcgggt ggtcgatgaa tccggtgccg aggtggcccc cggtgaagag ggtgaactgc tggtccgcgg ccccaacacg ctcaacgggt actaccgcga cgacgaggcc aacgcccgct cgttcagccc ggacgggttc taccgcaccg gtgaccgggt gcggatcttc gccgacggac cgcgggccgg ccatgtcgag gtgaccgggc gggtcaagga tgtgatccac cgcggcggag agaccgtgtc ggccaccgac ctcgaggacc atctgctcac ccatcccgcg atctacgccg ccgcggcggt cccgctcccc gatgagttcc tcggcgagaa gatctgcgcc gcagtggtgt tccgcggtgc tccggtctcg ctgaccgagc tcaacggcta tctcgacgag cgcggggtgg ccacccacgc ccgccccgac gtgctggtgg cgttgccgtc gctgccgatg accgcggtcg gcaaggtgga caagaagaag gtcgtcgagc aggtgctgtc gcagtga
Details for MythA.00629.l.B2.GE40029
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 59kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG FVPFPPDRVE EYRRAGYWTG RRLDSILSDA AAAQPDKAAV IDPDRSHTFA ELDALADRVA AGLADLGIAP GDRVLLQMPN DTRFAVAFFG LLRAGAIPVM CLPGHRSAEM SHFAEVSGAV GLIIPERANG FDYRELARAL TASRPRLRHV IVDGATDPSG DGFVPWSALA EFAGPAVPRP PVDTTAPALL LVSGGTTGLP KLIPRTHDDY VYTAVASARA YEMTSDDVYL VVLPAAHNFP LACPGMLGAM TVGASTVFTA DPSPEAAFPL INRHRVTVTG LVNALAKLWA QACEWEPELP ESLRVVQVGG SRMSPQEAQF ILDRLTPGMS QIFGMAEGML NFTRPGDPID VVVHTQGRPM SPHDEMRVVD ESGAEVAPGE EGELLVRGPN TLNGYYRDDE ANARSFSPDG FYRTGDRVRI FADGPRAGHV EVTGRVKDVI HRGGETVSAT DLEDHLLTHP AIYAAAAVPL PDEFLGEKIC AAVVFRGAPV SLTELNGYLD ERGVATHARP DVLVALPSLP MTAVGKVDKK K
Validated NT Sequence
ccttcttctt gtccaccttg ccgaccgcgg tcatcggcag cgacggcaac gccaccagca cgtcggggcg ggcgtgggtg gccaccccgc gctcgtcgag atagccgttg agctcggtca gcgagaccgg agcaccgcgg aacaccactg cggcgcagat cttctcgccg aggaactcat cggggagcgg gaccgccgcg gcggcgtaga tcgcgggatg ggtgagcaga tggtcctcga ggtcggtggc cgacacggtc tctccgccgc ggtggatcac atccttgacc cgcccggtca cctcgacatg gccggcccgc ggtccgtcgg cgaagatccg cacccggtca ccggtgcggt agaacccgtc cgggctgaac gagcgggcgt tggcctcgtc gtcgcggtag tacccgttga gcgtgttggg gccgcggacc agcagttcac cctcttcacc gggggccacc tcggcaccgg attcatcgac cacccgcatc tcgtcgtgcg gcgacatcgg ccggccctgg gtgtgcacca cgacgtcgat cgggtcaccg ggccgggtga agttcagcat gccctcggcc atcccgaaga tctgcgacat ccccggggtc agccggtcca ggatgaactg ggcctcctgc ggggacatcc gtgatccgcc gacctgcacc acccgcagtg actcgggcag ttccggttcc cactcgcagg cctgcgccca cagtttggcc agcgcgttga ccagcccggt caccgtgacc cgatgccggt tgatcagcgg gaacgccgcc tcggggctcg ggtcggcggt gaacacggtg gacgccccga ccgtcatcgc gcccagcatg cccggacagg ccagcgggaa gttgtgggcg gcaggcagca ccaccaggta cacgtcgtcg gacgtcatct cataggcccg ggcgctggcc accgcggtgt agacgtaatc gtcgtgggtc cgcgggatca gcttcggcag gccggtggtg ccccccgaga ccagcagcag ggccggcgcg gtggtgtcga ccggcgggcg ggggacggcc ggcccggcga actcggcgag tgcggaccac ggcacgaacc cgtcgcccga cgggtcggtg gccccgtcga cgatcacatg gcgcaaccgt ggacggctcg cggtgagcgc tctggccagt tcgcggtagt cgaagccgtt cgcccgctcc ggaatgatca gccccaccgc gccggacacc tcggcgaaat ggctcatctc ggccgaccgg tggcccggca ggcacatcac cgggatggcg cccgcccgca gcaacccgaa gaacgcgacc gcgaaccggg tgtcgttggg catctgcagc agcacccggt caccgggggc gatgccgaga tcggccagtc cggcggcgac ccggtcggcg agggcatcca gctcggcgaa ggtgtggctg cggtccgggt cgatcaccgc ggccttgtcg ggctgagccg ccgccgcgtc ggaaaggatg ctgtccagcc gacgtccggt ccagtacccg gcgcgtcgat actcctcgac ccggtccggt gggaacggga cgaatcccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHGF VPFPPDRVEE YRRAGYWTGR RLDSILSDAA AAQPDKAAVI DPDRSHTFAE LDALADRVAA GLADLGIAPG DRVLLQMPND TRFAVAFFGL LRAGAIPVMC LPGHRSAEMS HFAEVSGAVG LIIPERANGF DYRELARALT ASRPRLRHVI VDGATDPSGD GFVPWSALAE FAGPAVPRPP VDTTAPALLL VSGGTTGLPK LIPRTHDDYV YTAVASARAY EMTSDDVYLV VLPAAHNFPL ACPGMLGAMT VGASTVFTAD PSPEAAFPLI NRHRVTVTGL VNALAKLWAQ ACEWEPELPE SLRVVQVGGS RMSPQEAQFI LDRLTPGMSQ IFGMAEGMLN FTRPGDPIDV VVHTQGRPMS PHDEMRVVDE SGAEVAPGEE GELLVRGPNT LNGYYRDDEA NARSFSPDGF YRTGDRVRIF ADGPRAGHVE VTGRVKDVIH RGGETVSATD LEDHLLTHPA IYAAAAVPLP DEFLGEKICA AVVFRGAPVS LTELNGYLDE RGVATHARPD VLVALPSLPM TAVGKVDKKK VVEQVLSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggattcg tcccgttccc accggaccgg gtcgaggagt atcgacgcgc cgggtactgg accggacgtc ggctggacag catcctttcc gacgcggcgg cggctcagcc cgacaaggcc gcggtgatcg acccggaccg cagccacacc ttcgccgagc tggatgccct cgccgaccgg gtcgccgccg gactggccga tctcggcatc gcccccggtg accgggtgct gctgcagatg cccaacgaca cccggttcgc ggtcgcgttc ttcgggttgc tgcgggcggg cgccatcccg gtgatgtgcc tgccgggcca ccggtcggcc gagatgagcc atttcgccga ggtgtccggc gcggtggggc tgatcattcc ggagcgggcg aacggcttcg actaccgcga actggccaga gcgctcaccg cgagccgtcc acggttgcgc catgtgatcg tcgacggggc caccgacccg tcgggcgacg ggttcgtgcc gtggtccgca ctcgccgagt tcgccgggcc ggccgtcccc cgcccgccgg tcgacaccac cgcgccggcc ctgctgctgg tctcgggggg caccaccggc ctgccgaagc tgatcccgcg gacccacgac gattacgtct acaccgcggt ggccagcgcc cgggcctatg agatgacgtc cgacgacgtg tacctggtgg tgctgcctgc cgcccacaac ttcccgctgg cctgtccggg catgctgggc gcgatgacgg tcggggcgtc caccgtgttc accgccgacc cgagccccga ggcggcgttc ccgctgatca accggcatcg ggtcacggtg accgggctgg tcaacgcgct ggccaaactg tgggcgcagg cctgcgagtg ggaaccggaa ctgcccgagt cactgcgggt ggtgcaggtc ggcggatcac ggatgtcccc gcaggaggcc cagttcatcc tggaccggct gaccccgggg atgtcgcaga tcttcgggat ggccgagggc atgctgaact tcacccggcc cggtgacccg atcgacgtcg tggtgcacac ccagggccgg ccgatgtcgc cgcacgacga gatgcgggtg gtcgatgaat ccggtgccga ggtggccccc ggtgaagagg gtgaactgct ggtccgcggc cccaacacgc tcaacgggta ctaccgcgac gacgaggcca acgcccgctc gttcagcccg gacgggttct accgcaccgg tgaccgggtg cggatcttcg ccgacggacc gcgggccggc catgtcgagg tgaccgggcg ggtcaaggat gtgatccacc gcggcggaga gaccgtgtcg gccaccgacc tcgaggacca tctgctcacc catcccgcga tctacgccgc cgcggcggtc ccgctccccg atgagttcct cggcgagaag atctgcgccg cagtggtgtt ccgcggtgct ccggtctcgc tgaccgagct caacggctat ctcgacgagc gcggggtggc cacccacgcc cgccccgacg tgctggtggc gttgccgtcg ctgccgatga ccgcggtcgg caaggtggac aagaagaagg tcgtcgagca ggtgctgtcg cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MythA.00629.l.B2.PW37923
PURIFICATION DATe: 6/21/2016
CONCENTRATION: 21.5mg/ml
OBSERVED MW: 62kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG FVPFPPDRVE EYRRAGYWTG RRLDSILSDA AAAQPDKAAV IDPDRSHTFA ELDALADRVA AGLADLGIAP GDRVLLQMPN DTRFAVAFFG LLRAGAIPVM CLPGHRSAEM SHFAEVSGAV GLIIPERANG FDYRELARAL TASRPRLRHV IVDGATDPSG DGFVPWSALA EFAGPAVPRP PVDTTAPALL LVSGGTTGLP KLIPRTHDDY VYTAVASARA YEMTSDDVYL VVLPAAHNFP LACPGMLGAM TVGASTVFTA DPSPEAAFPL INRHRVTVTG LVNALAKLWA QACEWEPELP ESLRVVQVGG SRMSPQEAQF ILDRLTPGMS QIFGMAEGML NFTRPGDPID VVVHTQGRPM SPHDEMRVVD ESGAEVAPGE EGELLVRGPN TLNGYYRDDE ANARSFSPDG FYRTGDRVRI FADGPRAGHV EVTGRVKDVI HRGGETVSAT DLEDHLLTHP AIYAAAAVPL PDEFLGEKIC AAVVFRGAPV SLTELNGYLD ERGVATHARP DVLVALPSLP MTAVGKVDKK K
Validated NT Sequence
ccttcttctt gtccaccttg ccgaccgcgg tcatcggcag cgacggcaac gccaccagca cgtcggggcg ggcgtgggtg gccaccccgc gctcgtcgag atagccgttg agctcggtca gcgagaccgg agcaccgcgg aacaccactg cggcgcagat cttctcgccg aggaactcat cggggagcgg gaccgccgcg gcggcgtaga tcgcgggatg ggtgagcaga tggtcctcga ggtcggtggc cgacacggtc tctccgccgc ggtggatcac atccttgacc cgcccggtca cctcgacatg gccggcccgc ggtccgtcgg cgaagatccg cacccggtca ccggtgcggt agaacccgtc cgggctgaac gagcgggcgt tggcctcgtc gtcgcggtag tacccgttga gcgtgttggg gccgcggacc agcagttcac cctcttcacc gggggccacc tcggcaccgg attcatcgac cacccgcatc tcgtcgtgcg gcgacatcgg ccggccctgg gtgtgcacca cgacgtcgat cgggtcaccg ggccgggtga agttcagcat gccctcggcc atcccgaaga tctgcgacat ccccggggtc agccggtcca ggatgaactg ggcctcctgc ggggacatcc gtgatccgcc gacctgcacc acccgcagtg actcgggcag ttccggttcc cactcgcagg cctgcgccca cagtttggcc agcgcgttga ccagcccggt caccgtgacc cgatgccggt tgatcagcgg gaacgccgcc tcggggctcg ggtcggcggt gaacacggtg gacgccccga ccgtcatcgc gcccagcatg cccggacagg ccagcgggaa gttgtgggcg gcaggcagca ccaccaggta cacgtcgtcg gacgtcatct cataggcccg ggcgctggcc accgcggtgt agacgtaatc gtcgtgggtc cgcgggatca gcttcggcag gccggtggtg ccccccgaga ccagcagcag ggccggcgcg gtggtgtcga ccggcgggcg ggggacggcc ggcccggcga actcggcgag tgcggaccac ggcacgaacc cgtcgcccga cgggtcggtg gccccgtcga cgatcacatg gcgcaaccgt ggacggctcg cggtgagcgc tctggccagt tcgcggtagt cgaagccgtt cgcccgctcc ggaatgatca gccccaccgc gccggacacc tcggcgaaat ggctcatctc ggccgaccgg tggcccggca ggcacatcac cgggatggcg cccgcccgca gcaacccgaa gaacgcgacc gcgaaccggg tgtcgttggg catctgcagc agcacccggt caccgggggc gatgccgaga tcggccagtc cggcggcgac ccggtcggcg agggcatcca gctcggcgaa ggtgtggctg cggtccgggt cgatcaccgc ggccttgtcg ggctgagccg ccgccgcgtc ggaaaggatg ctgtccagcc gacgtccggt ccagtacccg gcgcgtcgat actcctcgac ccggtccggt gggaacggga cgaatcccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHGF VPFPPDRVEE YRRAGYWTGR RLDSILSDAA AAQPDKAAVI DPDRSHTFAE LDALADRVAA GLADLGIAPG DRVLLQMPND TRFAVAFFGL LRAGAIPVMC LPGHRSAEMS HFAEVSGAVG LIIPERANGF DYRELARALT ASRPRLRHVI VDGATDPSGD GFVPWSALAE FAGPAVPRPP VDTTAPALLL VSGGTTGLPK LIPRTHDDYV YTAVASARAY EMTSDDVYLV VLPAAHNFPL ACPGMLGAMT VGASTVFTAD PSPEAAFPLI NRHRVTVTGL VNALAKLWAQ ACEWEPELPE SLRVVQVGGS RMSPQEAQFI LDRLTPGMSQ IFGMAEGMLN FTRPGDPIDV VVHTQGRPMS PHDEMRVVDE SGAEVAPGEE GELLVRGPNT LNGYYRDDEA NARSFSPDGF YRTGDRVRIF ADGPRAGHVE VTGRVKDVIH RGGETVSATD LEDHLLTHPA IYAAAAVPLP DEFLGEKICA AVVFRGAPVS LTELNGYLDE RGVATHARPD VLVALPSLPM TAVGKVDKKK VVEQVLSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGATTCG TCCCGTTCCC ACCGGACCGG GTCGAGGAGT ATCGACGCGC CGGGTACTGG ACCGGACGTC GGCTGGACAG CATCCTTTCC GACGCGGCGG CGGCTCAGCC CGACAAGGCC GCGGTGATCG ACCCGGACCG CAGCCACACC TTCGCCGAGC TGGATGCCCT CGCCGACCGG GTCGCCGCCG GACTGGCCGA TCTCGGCATC GCCCCCGGTG ACCGGGTGCT GCTGCAGATG CCCAACGACA CCCGGTTCGC GGTCGCGTTC TTCGGGTTGC TGCGGGCGGG CGCCATCCCG GTGATGTGCC TGCCGGGCCA CCGGTCGGCC GAGATGAGCC ATTTCGCCGA GGTGTCCGGC GCGGTGGGGC TGATCATTCC GGAGCGGGCG AACGGCTTCG ACTACCGCGA ACTGGCCAGA GCGCTCACCG CGAGCCGTCC ACGGTTGCGC CATGTGATCG TCGACGGGGC CACCGACCCG TCGGGCGACG GGTTCGTGCC GTGGTCCGCA CTCGCCGAGT TCGCCGGGCC GGCCGTCCCC CGCCCGCCGG TCGACACCAC CGCGCCGGCC CTGCTGCTGG TCTCGGGGGG CACCACCGGC CTGCCGAAGC TGATCCCGCG GACCCACGAC GATTACGTCT ACACCGCGGT GGCCAGCGCC CGGGCCTATG AGATGACGTC CGACGACGTG TACCTGGTGG TGCTGCCTGC CGCCCACAAC TTCCCGCTGG CCTGTCCGGG CATGCTGGGC GCGATGACGG TCGGGGCGTC CACCGTGTTC ACCGCCGACC CGAGCCCCGA GGCGGCGTTC CCGCTGATCA ACCGGCATCG GGTCACGGTG ACCGGGCTGG TCAACGCGCT GGCCAAACTG TGGGCGCAGG CCTGCGAGTG GGAACCGGAA CTGCCCGAGT CACTGCGGGT GGTGCAGGTC GGCGGATCAC GGATGTCCCC GCAGGAGGCC CAGTTCATCC TGGACCGGCT GACCCCGGGG ATGTCGCAGA TCTTCGGGAT GGCCGAGGGC ATGCTGAACT TCACCCGGCC CGGTGACCCG ATCGACGTCG TGGTGCACAC CCAGGGCCGG CCGATGTCGC CGCACGACGA GATGCGGGTG GTCGATGAAT CCGGTGCCGA GGTGGCCCCC GGTGAAGAGG GTGAACTGCT GGTCCGCGGC CCCAACACGC TCAACGGGTA CTACCGCGAC GACGAGGCCA ACGCCCGCTC GTTCAGCCCG GACGGGTTCT ACCGCACCGG TGACCGGGTG CGGATCTTCG CCGACGGACC GCGGGCCGGC CATGTCGAGG TGACCGGGCG GGTCAAGGAT GTGATCCACC GCGGCGGAGA GACCGTGTCG GCCACCGACC TCGAGGACCA TCTGCTCACC CATCCCGCGA TCTACGCCGC CGCGGCGGTC CCGCTCCCCG ATGAGTTCCT CGGCGAGAAG ATCTGCGCCG CAGTGGTGTT CCGCGGTGCT CCGGTCTCGC TGACCGAGCT CAACGGCTAT CTCGACGAGC GCGGGGTGGC CACCCACGCC CGCCCCGACG TGCTGGTGGC GTTGCCGTCG CTGCCGATGA CCGCGGTCGG CAAGGTGGAC AAGAAGAAGG TCGTCGAGCA GGTGCTGTCG CAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for MythA.00629.l.B2.PW37995
PURIFICATION DATe: 12/12/2016
CONCENTRATION: 25mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG FVPFPPDRVE EYRRAGYWTG RRLDSILSDA AAAQPDKAAV IDPDRSHTFA ELDALADRVA AGLADLGIAP GDRVLLQMPN DTRFAVAFFG LLRAGAIPVM CLPGHRSAEM SHFAEVSGAV GLIIPERANG FDYRELARAL TASRPRLRHV IVDGATDPSG DGFVPWSALA EFAGPAVPRP PVDTTAPALL LVSGGTTGLP KLIPRTHDDY VYTAVASARA YEMTSDDVYL VVLPAAHNFP LACPGMLGAM TVGASTVFTA DPSPEAAFPL INRHRVTVTG LVNALAKLWA QACEWEPELP ESLRVVQVGG SRMSPQEAQF ILDRLTPGMS QIFGMAEGML NFTRPGDPID VVVHTQGRPM SPHDEMRVVD ESGAEVAPGE EGELLVRGPN TLNGYYRDDE ANARSFSPDG FYRTGDRVRI FADGPRAGHV EVTGRVKDVI HRGGETVSAT DLEDHLLTHP AIYAAAAVPL PDEFLGEKIC AAVVFRGAPV SLTELNGYLD ERGVATHARP DVLVALPSLP MTAVGKVDKK K
Validated NT Sequence
ccttcttctt gtccaccttg ccgaccgcgg tcatcggcag cgacggcaac gccaccagca cgtcggggcg ggcgtgggtg gccaccccgc gctcgtcgag atagccgttg agctcggtca gcgagaccgg agcaccgcgg aacaccactg cggcgcagat cttctcgccg aggaactcat cggggagcgg gaccgccgcg gcggcgtaga tcgcgggatg ggtgagcaga tggtcctcga ggtcggtggc cgacacggtc tctccgccgc ggtggatcac atccttgacc cgcccggtca cctcgacatg gccggcccgc ggtccgtcgg cgaagatccg cacccggtca ccggtgcggt agaacccgtc cgggctgaac gagcgggcgt tggcctcgtc gtcgcggtag tacccgttga gcgtgttggg gccgcggacc agcagttcac cctcttcacc gggggccacc tcggcaccgg attcatcgac cacccgcatc tcgtcgtgcg gcgacatcgg ccggccctgg gtgtgcacca cgacgtcgat cgggtcaccg ggccgggtga agttcagcat gccctcggcc atcccgaaga tctgcgacat ccccggggtc agccggtcca ggatgaactg ggcctcctgc ggggacatcc gtgatccgcc gacctgcacc acccgcagtg actcgggcag ttccggttcc cactcgcagg cctgcgccca cagtttggcc agcgcgttga ccagcccggt caccgtgacc cgatgccggt tgatcagcgg gaacgccgcc tcggggctcg ggtcggcggt gaacacggtg gacgccccga ccgtcatcgc gcccagcatg cccggacagg ccagcgggaa gttgtgggcg gcaggcagca ccaccaggta cacgtcgtcg gacgtcatct cataggcccg ggcgctggcc accgcggtgt agacgtaatc gtcgtgggtc cgcgggatca gcttcggcag gccggtggtg ccccccgaga ccagcagcag ggccggcgcg gtggtgtcga ccggcgggcg ggggacggcc ggcccggcga actcggcgag tgcggaccac ggcacgaacc cgtcgcccga cgggtcggtg gccccgtcga cgatcacatg gcgcaaccgt ggacggctcg cggtgagcgc tctggccagt tcgcggtagt cgaagccgtt cgcccgctcc ggaatgatca gccccaccgc gccggacacc tcggcgaaat ggctcatctc ggccgaccgg tggcccggca ggcacatcac cgggatggcg cccgcccgca gcaacccgaa gaacgcgacc gcgaaccggg tgtcgttggg catctgcagc agcacccggt caccgggggc gatgccgaga tcggccagtc cggcggcgac ccggtcggcg agggcatcca gctcggcgaa ggtgtggctg cggtccgggt cgatcaccgc ggccttgtcg ggctgagccg ccgccgcgtc ggaaaggatg ctgtccagcc gacgtccggt ccagtacccg gcgcgtcgat actcctcgac ccggtccggt gggaacggga cgaatcccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHGF VPFPPDRVEE YRRAGYWTGR RLDSILSDAA AAQPDKAAVI DPDRSHTFAE LDALADRVAA GLADLGIAPG DRVLLQMPND TRFAVAFFGL LRAGAIPVMC LPGHRSAEMS HFAEVSGAVG LIIPERANGF DYRELARALT ASRPRLRHVI VDGATDPSGD GFVPWSALAE FAGPAVPRPP VDTTAPALLL VSGGTTGLPK LIPRTHDDYV YTAVASARAY EMTSDDVYLV VLPAAHNFPL ACPGMLGAMT VGASTVFTAD PSPEAAFPLI NRHRVTVTGL VNALAKLWAQ ACEWEPELPE SLRVVQVGGS RMSPQEAQFI LDRLTPGMSQ IFGMAEGMLN FTRPGDPIDV VVHTQGRPMS PHDEMRVVDE SGAEVAPGEE GELLVRGPNT LNGYYRDDEA NARSFSPDGF YRTGDRVRIF ADGPRAGHVE VTGRVKDVIH RGGETVSATD LEDHLLTHPA IYAAAAVPLP DEFLGEKICA AVVFRGAPVS LTELNGYLDE RGVATHARPD VLVALPSLPM TAVGKVDKKK VVEQVLSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGATTCG TCCCGTTCCC ACCGGACCGG GTCGAGGAGT ATCGACGCGC CGGGTACTGG ACCGGACGTC GGCTGGACAG CATCCTTTCC GACGCGGCGG CGGCTCAGCC CGACAAGGCC GCGGTGATCG ACCCGGACCG CAGCCACACC TTCGCCGAGC TGGATGCCCT CGCCGACCGG GTCGCCGCCG GACTGGCCGA TCTCGGCATC GCCCCCGGTG ACCGGGTGCT GCTGCAGATG CCCAACGACA CCCGGTTCGC GGTCGCGTTC TTCGGGTTGC TGCGGGCGGG CGCCATCCCG GTGATGTGCC TGCCGGGCCA CCGGTCGGCC GAGATGAGCC ATTTCGCCGA GGTGTCCGGC GCGGTGGGGC TGATCATTCC GGAGCGGGCG AACGGCTTCG ACTACCGCGA ACTGGCCAGA GCGCTCACCG CGAGCCGTCC ACGGTTGCGC CATGTGATCG TCGACGGGGC CACCGACCCG TCGGGCGACG GGTTCGTGCC GTGGTCCGCA CTCGCCGAGT TCGCCGGGCC GGCCGTCCCC CGCCCGCCGG TCGACACCAC CGCGCCGGCC CTGCTGCTGG TCTCGGGGGG CACCACCGGC CTGCCGAAGC TGATCCCGCG GACCCACGAC GATTACGTCT ACACCGCGGT GGCCAGCGCC CGGGCCTATG AGATGACGTC CGACGACGTG TACCTGGTGG TGCTGCCTGC CGCCCACAAC TTCCCGCTGG CCTGTCCGGG CATGCTGGGC GCGATGACGG TCGGGGCGTC CACCGTGTTC ACCGCCGACC CGAGCCCCGA GGCGGCGTTC CCGCTGATCA ACCGGCATCG GGTCACGGTG ACCGGGCTGG TCAACGCGCT GGCCAAACTG TGGGCGCAGG CCTGCGAGTG GGAACCGGAA CTGCCCGAGT CACTGCGGGT GGTGCAGGTC GGCGGATCAC GGATGTCCCC GCAGGAGGCC CAGTTCATCC TGGACCGGCT GACCCCGGGG ATGTCGCAGA TCTTCGGGAT GGCCGAGGGC ATGCTGAACT TCACCCGGCC CGGTGACCCG ATCGACGTCG TGGTGCACAC CCAGGGCCGG CCGATGTCGC CGCACGACGA GATGCGGGTG GTCGATGAAT CCGGTGCCGA GGTGGCCCCC GGTGAAGAGG GTGAACTGCT GGTCCGCGGC CCCAACACGC TCAACGGGTA CTACCGCGAC GACGAGGCCA ACGCCCGCTC GTTCAGCCCG GACGGGTTCT ACCGCACCGG TGACCGGGTG CGGATCTTCG CCGACGGACC GCGGGCCGGC CATGTCGAGG TGACCGGGCG GGTCAAGGAT GTGATCCACC GCGGCGGAGA GACCGTGTCG GCCACCGACC TCGAGGACCA TCTGCTCACC CATCCCGCGA TCTACGCCGC CGCGGCGGTC CCGCTCCCCG ATGAGTTCCT CGGCGAGAAG ATCTGCGCCG CAGTGGTGTT CCGCGGTGCT CCGGTCTCGC TGACCGAGCT CAACGGCTAT CTCGACGAGC GCGGGGTGGC CACCCACGCC CGCCCCGACG TGCTGGTGGC GTTGCCGTCG CTGCCGATGA CCGCGGTCGG CAAGGTGGAC AAGAAGAAGG TCGTCGAGCA GGTGCTGTCG CAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT