MythA.00629.d

Acyl-CoA synthase (A0R2H4 homolog)

CENTER ID: MythA.00629.d
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.d.A2.GE29585 potential soluble domain 14 651
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.3329
RefSeq: WP_003926861.1
UniProt: G7CIJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEFDPDPAD PGATVEIEHL VEVIEPALGR QLLRHFVVLL EDALRRPDTP LSRLRLMXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXPPDAV AVVYEGRHYS YRELNEAANR VAHWLIGQGI GTEDRVAVLL DKSPELVITA LGVLKAGAVY LPIDPTYPDD RLSFILGDCD ARLVLREPVS DLDGQPTTDP TDTDRVRRLL PGNTAYLIYT SGTTGLPKGV PVPHRPVAEY FVWFKGDYQV DANDRLLQVA SPSFDVSIAE IFGTLACGAR LVIHRPGGLN DIGYLTALLQ DEGITAMHFV PSLLGLFLSL PGVNQWRTLQ RVPIGGEPLP GEVADKFHAT FDALLHNFYG PTETVINASR YKVEGKQGTR IVPIGRPKIN TQLHLLDNAL QPVPVGVIGE IYIGGTHVAH GYHRRPGLTA ERFVADPFNP GGRLYRSGDL ARRNADGDIE FVGRADEQVK IRGFRIELGD VAAAISVDPS VGQAVVVATD LPNVGRSLVG YLTPVSGEST DSVAVERIRA RVAAALPEYM VPAAYVVLDE IPITTHGKID RAALPEPQIA SMIEYREPAT DTEREVARLF GDLLGRSERI GADDSFFDLG GHSLLATKLV AAVRSAFGVD IGVQDVFELG TVARLAGRID ELRSGSGGPL RPRLTPQHFD GPAPLSSSQL RSWFAYRVDG PSVVNNIPFA XXXXXXXXXX XXXXXXXXXX XXXXGM
NT Sequence
ATGATCGAAT TCGACCCCGA CCCCGCCGAC CCGGGCGCGA CCGTCGAGAT CGAACACCTG GTGGAGGTCA TCGAACCCGC GCTGGGCCGC CAACTGCTCA GGCACTTCGT GGTGCTGCTC GAGGATGCGC TGCGCCGCCC GGACACCCCG CTGTCCCGCC TGCGGCTGAT GGNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCCCCC GGACGCCGTC GCCGTCGTCT ACGAGGGCCG GCACTACTCG TACCGGGAAC TCAACGAGGC CGCCAACCGG GTGGCCCACT GGCTGATCGG CCAGGGCATC GGCACCGAGG ACCGGGTCGC GGTACTGCTC GACAAGTCAC CCGAGCTCGT CATCACCGCG CTGGGTGTGC TGAAGGCCGG CGCGGTGTAC CTGCCGATCG ATCCCACCTA CCCCGACGAC CGGTTGTCGT TCATCCTGGG CGACTGCGAC GCCAGACTGG TGCTGCGCGA ACCCGTCTCG GACCTCGACG GGCAGCCGAC CACCGATCCG ACCGACACCG ACCGGGTGCG TCGGCTGCTG CCCGGCAACA CCGCGTACCT CATCTACACC TCGGGCACCA CCGGTCTGCC CAAGGGGGTT CCGGTGCCGC ACCGGCCGGT CGCGGAGTAC TTCGTCTGGT TCAAGGGCGA CTATCAGGTC GACGCGAACG ACCGGCTGCT GCAGGTGGCC TCCCCGAGCT TCGACGTGTC GATCGCCGAG ATCTTCGGCA CCCTGGCCTG CGGCGCCCGG CTGGTCATCC ACCGGCCCGG CGGGCTCAAC GACATCGGCT ACCTCACCGC CCTGCTGCAG GACGAAGGCA TCACCGCCAT GCACTTCGTG CCGTCGCTGC TGGGCCTGTT CCTGTCGCTG CCCGGGGTGA ACCAGTGGCG GACCCTGCAG CGGGTGCCCA TCGGCGGCGA ACCGTTGCCG GGTGAGGTGG CCGACAAGTT CCACGCCACC TTCGACGCCC TGTTGCACAA CTTCTACGGC CCGACCGAGA CCGTCATCAA CGCCAGCCGC TACAAGGTGG AAGGCAAGCA GGGCACCCGG ATCGTGCCGA TCGGCCGGCC GAAGATCAAC ACCCAGCTGC ACCTACTCGA CAACGCGCTG CAGCCGGTGC CCGTCGGCGT CATCGGTGAG ATCTACATCG GCGGAACCCA TGTCGCGCAC GGCTACCACC GGCGGCCCGG CCTGACCGCG GAGCGTTTCG TCGCCGACCC GTTCAACCCC GGCGGGCGGC TGTACCGGTC CGGCGACCTG GCCCGGCGCA ACGCCGACGG CGACATCGAA TTCGTCGGCC GCGCCGACGA GCAGGTGAAG ATCCGCGGAT TCCGGATCGA ACTCGGCGAC GTCGCGGCGG CGATCTCGGT GGATCCCAGC GTCGGTCAGG CGGTGGTGGT GGCCACCGAT CTGCCCAACG TCGGCCGGAG CCTGGTCGGA TATCTCACCC CGGTGAGCGG CGAGTCCACC GATTCGGTTG CGGTGGAACG CATCCGCGCG CGGGTCGCCG CCGCGCTGCC GGAGTACATG GTGCCGGCCG CCTACGTGGT GCTCGACGAG ATCCCGATCA CCACCCACGG CAAGATCGAC CGGGCGGCGC TGCCCGAACC GCAGATCGCC TCGATGATCG AGTACCGGGA GCCGGCCACC GACACCGAAC GCGAGGTCGC CCGGCTGTTC GGTGACCTGC TCGGCCGCTC CGAGCGCATC GGCGCCGACG ACTCGTTCTT CGACCTCGGC GGGCACTCCC TGCTGGCGAC GAAGCTCGTC GCGGCCGTGC GGTCTGCCTT CGGGGTGGAC ATCGGGGTGC AGGACGTCTT CGAACTGGGC ACGGTGGCCC GGCTGGCCGG ACGCATCGAC GAGTTGCGTT CGGGCAGCGG CGGACCGTTG CGCCCCCGGC TGACCCCACA GCATTTCGAC GGCCCGGCGC CGCTGTCCTC GTCGCAGCTC CGGTCCTGGT TCGCCTACCG TGTGGACGGC CCCAGCGTCG TCAACAACAT CCCGTTCGCN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGGATG
Details for MythA.00629.d.A2.GE29585
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 9
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVEIEHLV EVIEPALGRQ LLRHFVVLLE DALRRPDTPL SNN
Validated NT Sequence
ttgtttnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccgtc gagatcgaac acctggtgga ggtcatcgaa cccgcgctgg gccgccaact gctcaggcac ttcgtggtgc tgctcgagga tgcgctgcgc cgcccggaca ccccgctgag caataactag cannnannnn ngcannanct nnnnnnnnnn nnnnnnncnn nnnncgnnnn nnnnngnann nnnnnnnnnn nnannnnngc nnannnnnnn ancnnnannn nnnccancnn nnnnnntggn nngnncncnn cntgcnnann cngnnnannn nncgggnnnn cnnccnnnnc nnnggnnnnn nnannnnnnn nccntgnncc cnannatgnn cccgcnnnnt gannnnnang anntcnnnnc tgnnnnnnnc nncnnnnnnn nnacnnnnnt nnnnnnnngt naggngatcn gannnnnnnn nccgcgnnna nngacngnac attcncnnan nnnnnnncct acncntcncg cnnnattcct cnnnnnngan annncnnnnn ntcnngnnnn ttccnncntn nnnnnncnan naccncannn tnnnnnnnnn nnnnnnntnn ntng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVEIEHLV EVIEPALGRQ LLRHFVVLLE DALRRPDTPL SRLRLMXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPPDAVA VVYEGRHYSY RELNEAANRV AHWLIGQGIG TEDRVAVLLD KSPELVITAL GVLKAGAVYL PIDPTYPDDR LSFILGDCDA RLVLREPVSD LDGQPTTDPT DTDRVRRLLP GNTAYLIYTS GTTGLPKGVP VPHRPVAEYF VWFKGDYQVD ANDRLLQVAS PSFDVSIAEI FGTLACGARL VIHRPGGLND IGYLTALLQD EGITAMHFVP SLLGLFLSLP GVNQWRTLQR VPIGGEPLPG EVADKFHATF DALLHNFYGP TETVINASRY KVEGKQGTRI VPIGRPKINT QLHLLDNALQ PVPVGVIGEI YIGGTHVAHG YHRRPGLTAE RFVADPFNPG GRLYRSGDLA RRNADGDIEF VGRADEQVKI RGFRIELGDV AAAISVDPSV GQAVVVATDL PNVGRSLVGY LTPVSGESTD SVAVERIRAR VAAALPEYMV PAAYVVLDEI PITTHGKIDR AALPEPQIAS MIEYREPATD TEREVARLFG DLLGRSERIG ADDSFFDLGG HSLLATKLVA AVRSAFGVDI GVQDVFELGT VARLAGRIDE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgtcgag atcgaacacc tggtggaggt catcgaaccc gcgctgggcc gccaactgct caggcacttc gtggtgctgc tcgaggatgc gctgcgccgc ccggacaccc cgctgtcccg cctgcggctg atggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccc ccggacgccg tcgccgtcgt ctacgagggc cggcactact cgtaccggga actcaacgag gccgccaacc gggtggccca ctggctgatc ggccagggca tcggcaccga ggaccgggtc gcggtactgc tcgacaagtc acccgagctc gtcatcaccg cgctgggtgt gctgaaggcc ggcgcggtgt acctgccgat cgatcccacc taccccgacg accggttgtc gttcatcctg ggcgactgcg acgccagact ggtgctgcgc gaacccgtct cggacctcga cgggcagccg accaccgatc cgaccgacac cgaccgggtg cgtcggctgc tgcccggcaa caccgcgtac ctcatctaca cctcgggcac caccggtctg cccaaggggg ttccggtgcc gcaccggccg gtcgcggagt acttcgtctg gttcaagggc gactatcagg tcgacgcgaa cgaccggctg ctgcaggtgg cctccccgag cttcgacgtg tcgatcgccg agatcttcgg caccctggcc tgcggcgccc ggctggtcat ccaccggccc ggcgggctca acgacatcgg ctacctcacc gccctgctgc aggacgaagg catcaccgcc atgcacttcg tgccgtcgct gctgggcctg ttcctgtcgc tgcccggggt gaaccagtgg cggaccctgc agcgggtgcc catcggcggc gaaccgttgc cgggtgaggt ggccgacaag ttccacgcca ccttcgacgc cctgttgcac aacttctacg gcccgaccga gaccgtcatc aacgccagcc gctacaaggt ggaaggcaag cagggcaccc ggatcgtgcc gatcggccgg ccgaagatca acacccagct gcacctactc gacaacgcgc tgcagccggt gcccgtcggc gtcatcggtg agatctacat cggcggaacc catgtcgcgc acggctacca ccggcggccc ggcctgaccg cggagcgttt cgtcgccgac ccgttcaacc ccggcgggcg gctgtaccgg tccggcgacc tggcccggcg caacgccgac ggcgacatcg aattcgtcgg ccgcgccgac gagcaggtga agatccgcgg attccggatc gaactcggcg acgtcgcggc ggcgatctcg gtggatccca gcgtcggtca ggcggtggtg gtggccaccg atctgcccaa cgtcggccgg agcctggtcg gatatctcac cccggtgagc ggcgagtcca ccgattcggt tgcggtggaa cgcatccgcg cgcgggtcgc cgccgcgctg ccggagtaca tggtgccggc cgcctacgtg gtgctcgacg agatcccgat caccacccac ggcaagatcg accgggcggc gctgcccgaa ccgcagatcg cctcgatgat cgagtaccgg gagccggcca ccgacaccga acgcgaggtc gcccggctgt tcggtgacct gctcggccgc tccgagcgca tcggcgccga cgactcgttc ttcgacctcg gcgggcactc cctgctggcg acgaagctcg tcgcggccgt gcggtctgcc ttcggggtgg acatcggggt gcaggacgtc ttcgaactgg gcacggtggc ccggctggcc ggacgcatcg acgagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa