MythA.00629.c

Acyl-CoA synthase (A0R2H4 homolog)

CENTER ID: MythA.00629.c
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.c.A1.GE28746 Full length( MythA.00629.c ) 1 544
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.2883
RefSeq: WP_003926416.1
UniProt: G7CH42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASPTRCDR NRDRTDLVGV RAVPGADTHH RSSSLLPQRW CMGIEIVLDM AVSAMADRVA VGRRDSGLTY RQLDDMAAGG AGLLRDRGVE HLVFVGVNGP IIPVLMFAAA KAGIPLAPMN YRLPSDQMRA LIAQLDRPMI VTDSAYRADL EGASEHVLTS DEFVATARGA GRVDHPPAPD DGTAIVLFTS GTTSAPKGVL LRHHQLSAYL FNTVEFGAAG DEEAMLISTP PYHIAGMGAV LSNLYAGRRI VYLPDFTAAD WLATVREERI TNAMVVPTML ARIVEYLDGE PARVPTLASL AYGGARLPQS VLERALRAFP DTGFIXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXSQLRH RLSSAGRIVP GVEVQIRDED GTTVLPEGET GLLWVRGAQV SGEYLGSGSV LDGSGWFPTR DRARIEDGFL YIGGRADDTI IRGGENISPA EIEDVLVHHD GVREVAVVGL PDEEWGERIC AVIVPVDGSH AEPDQVREWC HARLRGSRTP DEVVFVDDLP KNATGKVLRR DIVEKLTEGV AMSG
NT Sequence
ATTACGGCAT CTCCAACGAG GTGTGACCGC AACCGCGATC GCACCGACCT GGTGGGTGTC CGGGCTGTGC CCGGCGCGGA CACCCACCAT CGGTCGTCAT CCCTACTTCC TCAGAGGTGG TGCATGGGAA TCGAAATCGT GCTCGACATG GCGGTGTCCG CCATGGCCGA CCGGGTCGCG GTAGGCCGCC GGGACTCCGG CCTCACCTAC CGTCAGCTCG ACGACATGGC CGCCGGGGGC GCCGGCCTGT TGCGGGACCG CGGTGTGGAG CACCTCGTCT TCGTGGGGGT CAACGGGCCG ATCATCCCCG TCCTCATGTT CGCAGCGGCG AAGGCCGGAA TACCCCTGGC GCCCATGAAC TATCGGCTGC CGTCGGATCA GATGCGGGCG CTCATCGCGC AACTGGACCG CCCGATGATC GTCACCGACA GCGCCTACCG GGCAGATCTC GAGGGCGCGT CCGAGCATGT GCTCACCAGT GACGAGTTCG TCGCGACCGC GCGGGGGGCC GGACGGGTCG ATCACCCACC GGCGCCCGAC GACGGCACCG CGATCGTGTT GTTCACCTCA GGGACGACGT CGGCCCCCAA GGGGGTGCTG CTCCGGCATC ACCAGCTGTC GGCCTATCTG TTCAACACAG TCGAATTCGG CGCTGCCGGT GACGAAGAGG CCATGCTGAT CAGTACCCCG CCCTACCACA TCGCCGGGAT GGGGGCGGTG CTGAGCAACC TCTACGCCGG CCGGCGAATC GTGTACCTGC CGGACTTCAC CGCGGCGGAT TGGCTGGCCA CCGTCCGCGA GGAGCGGATC ACGAACGCGA TGGTGGTTCC GACGATGCTC GCGCGCATCG TCGAGTATCT GGACGGTGAG CCTGCGCGGG TGCCGACCCT CGCGTCGTTG GCGTACGGCG GCGCCCGGTT GCCCCAGTCG GTTCTCGAGC GCGCGCTGCG GGCATTTCCC GACACCGGGT TCATCNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTTTCGCA GCTGCGGCAC CGGCTGTCCT CTGCCGGCCG GATCGTGCCA GGGGTCGAGG TCCAGATCCG CGATGAGGAC GGCACCACCG TCCTCCCGGA GGGGGAGACC GGTCTGCTGT GGGTGCGGGG TGCTCAGGTC AGCGGCGAGT ATCTGGGCAG CGGCTCGGTC CTGGACGGCT CCGGCTGGTT CCCCACCCGC GATCGGGCGC GCATCGAGGA CGGATTCCTC TACATCGGTG GACGGGCCGA CGACACCATC ATCCGTGGTG GGGAGAACAT CTCGCCCGCC GAGATCGAGG ATGTGCTGGT CCACCACGAC GGTGTGCGGG AGGTCGCGGT CGTCGGTCTG CCGGACGAGG AGTGGGGCGA ACGCATCTGT GCGGTGATCG TTCCGGTCGA CGGTTCGCAC GCCGAACCGG ATCAGGTGCG GGAGTGGTGC CATGCCCGCC TGCGTGGCTC ACGCACGCCG GACGAGGTGG TGTTCGTCGA TGACCTGCCG AAGAACGCCA CCGGGAAAGT GTTGCGACGC GACATTGTCG AGAAACTGAC CGAGGGAGTC GCGATGAGCG GC
Details for MythA.00629.c.A1.GE28746
HARVESTED ON: 3/1/2010
SEQUENCED ON: 3/5/2010
EXPECTED MW: 61kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 94
PERCENT COVERAGE: 54
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMITASPTRC DRNRDRTDLV GVRAVPGADT HHRSSSLLPQ RWCMGIEIVL DMAVSAMADR VAVGRRDSGL TYRQLDDMAA GGAGLLRDRG VEHLVFVGVN GPIIPVLMFA AAKAGIPLAP MNYRLPSDQM RALIAQLDRP MIVTDSAYRA DLEGASEHVL TSDEFVATAR GAGRVDHPPA PDDGTAIVLF TSGTTSAPKG VLLRHHQLSA YLFNTVEFGA AGDEEAMLIS TPPYHIAXMG AVLSNLYAGR RIVYLPXFTA ADWLXHRPRG ADHERDGGSD DARAHRRVSG R
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattacggca tctccaacga ggtgtgaccg caaccgcgat cgcaccgacc tggtgggtgt ccgggctgtg cccggcgcgg acacccacca tcggtcgtca tccctacttc ctcagaggtg gtgcatggga atcgaaatcg tgctcgacat ggcggtgtcc gccatggccg accgggtcgc ggtaggccgc cgggactccg gcctcaccta ccgtcagctc gacgacatgg ccgccggggg cgccggcctg ttgcgggacc gcggtgtgga gcacctcgtc ttcgtggggg tcaacgggcc gatcatcccc gtcctcatgt tcgcagcggc gaaggccgga atacccctgg cgcccatgaa ctatcggctg ccgtcggatc agatgcgggc gctcatcgcg caactggacc gcccgatgat cgtcaccgac agcgcctacc gggcagatct cgagggcgcg tccgagcatg tgctcaccag tgacgagttc gtcgcgaccg cgcggggggc cggacgggtc gatcacccac cggcgcccga cgacggcacc gcgatcgtgt tgttcacctc agggacgacg tcggccccca agggggtgct gctccggcat caccagctgt cggcctatct gttcaacaca gtcgaattcg gcgctgccgg ngacgaagag gccatgctga tcagtacccc gccctaccac atcgccngga tgggggcggt gctgagcaac ctctacgccg gccggcgaat cgtgtacctg ccgganttca ccgcggcgga ttggctggnc caccgtccgc gaggagcgga tcacgaacgc gatggtggtt ccgacgatgc tcgcgcgcat cgtcgagtat ctggacggtg agcctgcgcg ggtgcngacc ctcgcgtcgt tggcgtangg cggcgcccgg ttgccccagt cggttctcga gcgcgcgctg cggncatttc ccgacaccgg gttcatcaac gcntacggtc tcaccgagac cagctcgacg atcgcggtgc tgggaccgga ggaacaccgc gatgcgatgt ctgattcgca gctgcggcac cggctgtcct ctgccggccg gatcgtgcca ggggtcgagg tccagatccg cgatgaggac ggcaccaccg tcctcccgga gggggagacc ggtctgctgt gggtgcgggg tgctcaggtc agcggcgagt atctgggcag cggctcggtc ctggacggct ccggctggtt ccccacccgc gatcgggcgc gcatcgagga cggattcctc tacatcggtg gacgggccga cgacaccatc atccgtggtg gggagaacat ctcgcccgcc gagatcgagg atgtgctggt ccaccacgac ggtgtgcggg aggtcgcggt cgtcggtctg ccggacgagg agtggggcga acgcatctgt gcggtgatcg ttccggtcga cggttcgcac gccgaaccgg atcaggtgcg ggagtggtgc catgcccgcc tgcgtggctc acgcacgccg gacgaggtgg tgttcgtcga tgacctgccg aagaacgcca ccgggaaagt gttgcgacgc gacattgtcg agaaactgac cgagggagtc gcgatgagcg gctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMITASPTRC DRNRDRTDLV GVRAVPGADT HHRSSSLLPQ RWCMGIEIVL DMAVSAMADR VAVGRRDSGL TYRQLDDMAA GGAGLLRDRG VEHLVFVGVN GPIIPVLMFA AAKAGIPLAP MNYRLPSDQM RALIAQLDRP MIVTDSAYRA DLEGASEHVL TSDEFVATAR GAGRVDHPPA PDDGTAIVLF TSGTTSAPKG VLLRHHQLSA YLFNTVEFGA AGDEEAMLIS TPPYHIAGMG AVLSNLYAGR RIVYLPDFTA ADWLATVREE RITNAMVVPT MLARIVEYLD GEPARVPTLA SLAYGGARLP QSVLERALRA FPDTGFIXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXSQL RHRLSSAGRI VPGVEVQIRD EDGTTVLPEG ETGLLWVRGA QVSGEYLGSG SVLDGSGWFP TRDRARIEDG FLYIGGRADD TIIRGGENIS PAEIEDVLVH HDGVREVAVV GLPDEEWGER ICAVIVPVDG SHAEPDQVRE WCHARLRGSR TPDEVVFVDD LPKNATGKVL RRDIVEKLTE GVAMSG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattacggca tctccaacga ggtgtgaccg caaccgcgat cgcaccgacc tggtgggtgt ccgggctgtg cccggcgcgg acacccacca tcggtcgtca tccctacttc ctcagaggtg gtgcatggga atcgaaatcg tgctcgacat ggcggtgtcc gccatggccg accgggtcgc ggtaggccgc cgggactccg gcctcaccta ccgtcagctc gacgacatgg ccgccggggg cgccggcctg ttgcgggacc gcggtgtgga gcacctcgtc ttcgtggggg tcaacgggcc gatcatcccc gtcctcatgt tcgcagcggc gaaggccgga atacccctgg cgcccatgaa ctatcggctg ccgtcggatc agatgcgggc gctcatcgcg caactggacc gcccgatgat cgtcaccgac agcgcctacc gggcagatct cgagggcgcg tccgagcatg tgctcaccag tgacgagttc gtcgcgaccg cgcggggggc cggacgggtc gatcacccac cggcgcccga cgacggcacc gcgatcgtgt tgttcacctc agggacgacg tcggccccca agggggtgct gctccggcat caccagctgt cggcctatct gttcaacaca gtcgaattcg gcgctgccgg tgacgaagag gccatgctga tcagtacccc gccctaccac atcgccggga tgggggcggt gctgagcaac ctctacgccg gccggcgaat cgtgtacctg ccggacttca ccgcggcgga ttggctggcc accgtccgcg aggagcggat cacgaacgcg atggtggttc cgacgatgct cgcgcgcatc gtcgagtatc tggacggtga gcctgcgcgg gtgccgaccc tcgcgtcgtt ggcgtacggc ggcgcccggt tgccccagtc ggttctcgag cgcgcgctgc gggcatttcc cgacaccggg ttcatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttcgc agctgcggca ccggctgtcc tctgccggcc ggatcgtgcc aggggtcgag gtccagatcc gcgatgagga cggcaccacc gtcctcccgg agggggagac cggtctgctg tgggtgcggg gtgctcaggt cagcggcgag tatctgggca gcggctcggt cctggacggc tccggctggt tccccacccg cgatcgggcg cgcatcgagg acggattcct ctacatcggt ggacgggccg acgacaccat catccgtggt ggggagaaca tctcgcccgc cgagatcgag gatgtgctgg tccaccacga cggtgtgcgg gaggtcgcgg tcgtcggtct gccggacgag gagtggggcg aacgcatctg tgcggtgatc gttccggtcg acggttcgca cgccgaaccg gatcaggtgc gggagtggtg ccatgcccgc ctgcgtggct cacgcacgcc ggacgaggtg gtgttcgtcg atgacctgcc gaagaacgcc accgggaaag tgttgcgacg cgacattgtc gagaaactga ccgagggagt cgcgatgagc ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa