MythA.00629.a

Acyl-CoA synthase (A0R2H4 homolog)

CENTER ID: MythA.00629.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00629.a.A1.GE28744 Full length( MythA.00629.a ) 1 581
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.912
RefSeq: WP_003924400.1
UniProt: G7CD72

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFRFDAVTY PGAMAKLTXX XXXLAADKLP LSRLQLYAER GAAELHYLRK MLEAGALKIE SPTRIAALLA DIRRWGEIGM IPALNARRWP DRVAVIDDEG EMTFAELNDA AHAVANALLA KGVRXXXXXX XXAILARNHR WFLVSMYGAA RVGARIILLN TEFSGPQICE VSDREGAKLI IYDDEYTAAV SGVEPELGKL RALGHNPDSD EPSGSTDETL ADIIERVGAK PAPKATRHSS IIILTSGTTG TPKGATRKAP PSLAPIGGVL SHVPFKSGEV TSLPAPMFHA LGYLHATIAM MLGNTLLLRR RFKPATVLTD IEKHNVSAVI VVPVMLSRML DEWEKMRPKP DLSSLRIVFV SGSQLGAELA SRAMRDLGPV IYNLYGSTEI AFATIARPQD LSINPATVGP VVKGVTVKLF DENGKEVPRG EVGRIFVRNT FPFEGYTGGG HKQIIDGLMS SGDVGYFDEH GLLYVSGRDD EMIVSGGENV FPAEIEDLLS GHPEIVEATA IGVEDKEWGQ RLRAFVVKAE GSDLDEDEVK RYVREHLARY KVPRDVIFLD ELPRNPTGKI LKRELRELEV S
NT Sequence
CTGGGATTCC GGTTCGACGC GGTCACATAT CCTGGCGCTA TGGCAAAGCT GACCGANNNN NNNNNNNNNC TCGCGGCGGA CAAGCTGCCG CTCTCCAGAC TTCAGCTTTA CGCCGAACGT GGAGCGGCGG AGCTGCACTA TCTCCGCAAG ATGCTGGAAG CCGGTGCGCT GAAGATCGAG TCGCCGACCA GGATCGCCGC GCTGCTGGCC GACATCCGGC GGTGGGGCGA GATCGGGATG ATCCCGGCCC TCAACGCGCG CCGGTGGCCC GACCGGGTCG CGGTCATCGA CGACGAGGGC GAGATGACGT TCGCAGAACT CAACGACGCC GCGCACGCGG TGGCCAACGC GCTGCTGGCG AAGGGCGTCA GGNNNNNNNN NNNNNNNNNN NNNNTGGCCA TCCTGGCCCG CAACCACCGC TGGTTCCTGG TCTCGATGTA CGGCGCCGCC CGCGTCGGCG CCCGGATCAT CCTGCTCAAC ACCGAGTTCT CCGGGCCGCA GATCTGCGAG GTGTCCGATC GGGAGGGCGC CAAGCTGATC ATCTACGACG ACGAGTACAC CGCCGCGGTG TCGGGGGTCG AGCCGGAGCT GGGCAAGCTG CGCGCCCTGG GCCACAACCC GGACTCCGAC GAGCCGTCCG GCAGCACCGA CGAGACGCTG GCCGACATCA TCGAACGGGT CGGGGCGAAA CCCGCCCCGA AGGCCACCAG ACACTCGTCG ATCATCATCC TGACCAGCGG GACCACCGGC ACCCCCAAGG GCGCCACCCG CAAGGCGCCG CCGTCGCTGG CGCCGATCGG CGGGGTGCTC TCCCACGTGC CGTTCAAGTC CGGTGAGGTC ACCTCGCTGC CGGCGCCGAT GTTCCACGCG CTGGGCTACC TGCACGCGAC GATCGCGATG ATGCTGGGCA ACACGCTGTT GCTGCGGCGC CGGTTCAAGC CCGCGACGGT GCTGACCGAC ATCGAGAAGC ACAACGTCAG CGCGGTCATC GTGGTGCCGG TGATGCTGTC GCGGATGCTC GACGAGTGGG AGAAGATGCG GCCCAAACCG GACCTGTCGA GCCTACGCAT CGTGTTCGTC TCCGGCTCCC AGTTGGGCGC CGAACTGGCC TCCCGCGCGA TGCGCGACCT GGGCCCGGTG ATCTACAACC TGTACGGGTC GACCGAGATC GCCTTCGCCA CCATCGCCCG GCCGCAGGAT CTGTCGATCA ACCCGGCCAC GGTCGGGCCG GTCGTCAAGG GTGTGACGGT CAAACTGTTC GACGAGAACG GCAAGGAGGT GCCGCGCGGC GAGGTGGGCC GCATCTTCGT GCGCAACACG TTCCCGTTCG AGGGCTACAC CGGCGGCGGG CACAAGCAGA TCATCGACGG CCTGATGTCC TCCGGCGACG TCGGCTATTT CGACGAGCAC GGTCTGCTCT ACGTCAGCGG TCGCGACGAC GAGATGATCG TCTCCGGTGG GGAGAACGTG TTCCCGGCCG AGATCGAGGA TCTGCTCAGC GGGCATCCCG AGATCGTCGA GGCCACCGCG ATCGGCGTGG AGGACAAGGA GTGGGGGCAG CGGCTGCGGG CCTTCGTGGT CAAGGCCGAG GGCTCCGACC TCGACGAGGA CGAGGTGAAG CGCTACGTGC GCGAGCACCT GGCCCGCTAC AAGGTGCCCC GCGACGTGAT TTTCCTCGAC GAGTTGCCCC GCAACCCCAC CGGCAAGATC CTCAAACGCG AACTGCGCGA ACTCGAGGTT TCC
Details for MythA.00629.a.A1.GE28744
HARVESTED ON: 3/1/2010
SEQUENCED ON: 5/19/2010
EXPECTED MW: 65kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 26
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLGFRFDAV TYPGAMAKLT DLPRLAADKL PLSRLQLYAE RGAAELHYLR KMLEAGALKI ESPTRIAALL ADIRRWGEIG MIPALNARRW PDRVAVIDDE GEMTFAELND AAHAVANALL AKGVRGGDGV AILARNHR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgg gattccggtt cgacgcggtc acatatcctg gcgctatggc aaagctgacc gatcttcccc ggctcgcggc ggacaagctg ccgctctcca gacttcagct ttacgccgaa cgtggagcgg cggagctgca ctatctccgc aagatgctgg aagccggtgc gctgaagatc gagtcgccga ccaggatcgc cgcgctgctg gccgacatcc ggcggtgggg cgagatcggg atgatcccgg ccctcaacgc gcgccggtgg cccgaccggg tcgcggtcat cgacgacgag ggcgagatga cgttcgcaga actcaacgac gccgcgcacg cggtggccaa cgcgctgctg gcgaagggcg tcaggggcgg ggacggggtg gccatcctgg cccgcaacca ccgctga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGFRFDAV TYPGAMAKLT XXXXXLAADK LPLSRLQLYA ERGAAELHYL RKMLEAGALK IESPTRIAAL LADIRRWGEI GMIPALNARR WPDRVAVIDD EGEMTFAELN DAAHAVANAL LAKGVRXXXX XXXXAILARN HRWFLVSMYG AARVGARIIL LNTEFSGPQI CEVSDREGAK LIIYDDEYTA AVSGVEPELG KLRALGHNPD SDEPSGSTDE TLADIIERVG AKPAPKATRH SSIIILTSGT TGTPKGATRK APPSLAPIGG VLSHVPFKSG EVTSLPAPMF HALGYLHATI AMMLGNTLLL RRRFKPATVL TDIEKHNVSA VIVVPVMLSR MLDEWEKMRP KPDLSSLRIV FVSGSQLGAE LASRAMRDLG PVIYNLYGST EIAFATIARP QDLSINPATV GPVVKGVTVK LFDENGKEVP RGEVGRIFVR NTFPFEGYTG GGHKQIIDGL MSSGDVGYFD EHGLLYVSGR DDEMIVSGGE NVFPAEIEDL LSGHPEIVEA TAIGVEDKEW GQRLRAFVVK AEGSDLDEDE VKRYVREHLA RYKVPRDVIF LDELPRNPTG KILKRELREL EVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgggattc cggttcgacg cggtcacata tcctggcgct atggcaaagc tgaccgannn nnnnnnnnnn ctcgcggcgg acaagctgcc gctctccaga cttcagcttt acgccgaacg tggagcggcg gagctgcact atctccgcaa gatgctggaa gccggtgcgc tgaagatcga gtcgccgacc aggatcgccg cgctgctggc cgacatccgg cggtggggcg agatcgggat gatcccggcc ctcaacgcgc gccggtggcc cgaccgggtc gcggtcatcg acgacgaggg cgagatgacg ttcgcagaac tcaacgacgc cgcgcacgcg gtggccaacg cgctgctggc gaagggcgtc aggnnnnnnn nnnnnnnnnn nnnnntggcc atcctggccc gcaaccaccg ctggttcctg gtctcgatgt acggcgccgc ccgcgtcggc gcccggatca tcctgctcaa caccgagttc tccgggccgc agatctgcga ggtgtccgat cgggagggcg ccaagctgat catctacgac gacgagtaca ccgccgcggt gtcgggggtc gagccggagc tgggcaagct gcgcgccctg ggccacaacc cggactccga cgagccgtcc ggcagcaccg acgagacgct ggccgacatc atcgaacggg tcggggcgaa acccgccccg aaggccacca gacactcgtc gatcatcatc ctgaccagcg ggaccaccgg cacccccaag ggcgccaccc gcaaggcgcc gccgtcgctg gcgccgatcg gcggggtgct ctcccacgtg ccgttcaagt ccggtgaggt cacctcgctg ccggcgccga tgttccacgc gctgggctac ctgcacgcga cgatcgcgat gatgctgggc aacacgctgt tgctgcggcg ccggttcaag cccgcgacgg tgctgaccga catcgagaag cacaacgtca gcgcggtcat cgtggtgccg gtgatgctgt cgcggatgct cgacgagtgg gagaagatgc ggcccaaacc ggacctgtcg agcctacgca tcgtgttcgt ctccggctcc cagttgggcg ccgaactggc ctcccgcgcg atgcgcgacc tgggcccggt gatctacaac ctgtacgggt cgaccgagat cgccttcgcc accatcgccc ggccgcagga tctgtcgatc aacccggcca cggtcgggcc ggtcgtcaag ggtgtgacgg tcaaactgtt cgacgagaac ggcaaggagg tgccgcgcgg cgaggtgggc cgcatcttcg tgcgcaacac gttcccgttc gagggctaca ccggcggcgg gcacaagcag atcatcgacg gcctgatgtc ctccggcgac gtcggctatt tcgacgagca cggtctgctc tacgtcagcg gtcgcgacga cgagatgatc gtctccggtg gggagaacgt gttcccggcc gagatcgagg atctgctcag cgggcatccc gagatcgtcg aggccaccgc gatcggcgtg gaggacaagg agtgggggca gcggctgcgg gccttcgtgg tcaaggccga gggctccgac ctcgacgagg acgaggtgaa gcgctacgtg cgcgagcacc tggcccgcta caaggtgccc cgcgacgtga ttttcctcga cgagttgccc cgcaacccca ccggcaagat cctcaaacgc gaactgcgcg aactcgaggt ttccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa