MythA.00612.a
Lysine--tRNA ligase LysS (EC 6.1.1.6) (Lysyl-tRNA synthetase)
| CENTER ID: | MythA.00612.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00612.a.B1.GE40759 | full length | 1 | 500 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.00612.a.B1.PW38257 | full length | 1 | 500 |
Structures
6AQH
DEPOSITED: 8/19/2017
DETERMINATION: XRay
CLONE: MythA.00612.a.B1.GE40759
PROTEIN: MythA.00612.a.B1.PW38257
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.1556 |
| UniProt: | G7CF12 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPPESVDPD LPEQFRIRQA KRADLLAQGR QPYPVDVPRT HTLLEIRQAY PDLPVDARTG EIVGVTGRVV FARNSGKLCF ATLQEGDGTQ LQAMISLAEV GQEALDNWKT YVDIGDIVFV HGEVITSKRG ELSVLADSWQ MAAKALRPLP VAHKEMSEES RVRQRYVDLI VRDQARKVAR QRIAVMRAIR SALERRGFLE VETPMLQTLA GGAAARPFVT HSNALDTELY LRIAPELFLK RCLVGGFERV FELNRVFRNE GADSTHSPEF VMLETYQAYG TYDDSAVVTR ELIQEVADEA IGTRQVPLPD GTVYDLDGEW ESIQMYPSLS EALGEEITPD TPAETLWAIA DRLGLDIPRD RGYGHGKLVE ELWEHTVGAK LWAPTFVKDF PVETTPLTRS HRSIPGVTEK WDLYVRRIEL ATGYSELTDP IIQRERFEAQ ARAAAAGDDE AMALDEDFLA ALEYGMPPST GTGMGIDRLM MTLTGLSIRE TVLFPIVKPR
NT Sequence
gtgactccac ccgaatcggt tgatcctgac ctgcccgaac aattccgcat tcgccaggcc aagcgtgcgg acctgctcgc tcagggccgg cagccgtatc cggtcgacgt gccgcggacg cacaccctgc tcgagatccg gcaggcctac cccgacctgc ccgtcgacgc gcgcaccggc gagatcgtag gcgtcaccgg ccgggtggtg ttcgcccgga attccggcaa gctgtgcttc gcgaccctgc aggaaggcga cggcacccag ctacaggcga tgatcagtct cgccgaggtg gggcaggaag cgctggacaa ctggaagacc tacgtcgaca tcggcgacat cgtcttcgtg cacggcgagg tgatcacctc gaaacgcggc gagctgtctg tgctcgccga ctcctggcaa atggccgcca aggcgctgcg gccattgccg gtcgcgcaca aggagatgtc ggaggaatcc cgggtccggc agcgttacgt ggacctcatc gtgcgcgatc aggcccggaa ggtggcgcgg caacgaatcg cggtgatgcg cgcgatccgt tcggcgctgg agcggcgcgg attcctcgaa gtcgagacgc cgatgttgca gacattggcc ggtggtgcgg cggcccgccc gttcgtcacc cactcaaatg ccctcgacac cgagctttat ctgcggattg cgccggagtt gttcctcaag cgctgcctgg tcggcggttt cgagcgggtc ttcgaactga atcgggtgtt ccgaaacgaa ggcgcggatt ccacacattc cccggaattc gtgatgttgg agacttatca ggcgtacggc acctacgacg attccgcggt ggtgacccgg gaattaattc aggaagtcgc cgacgaggcc atcggcaccc gacaagtgcc gttgcccgat ggcacggtct acgacctcga cggggaatgg gagtcgattc aaatgtatcc ctccctgtcg gaggcgctgg gcgaggagat cacccccgac acgccggccg agacgttgtg ggcgatcgcg gaccggttgg ggttggatat tcccagggat cgtggttacg ggcacggcaa gctcgtcgag gagctgtggg agcacaccgt cggtgcgaaa ctgtgggcgc ccaccttcgt caaggatttc ccggtggaga ccacgccgtt gacgagatcg caccgcagta tccccggggt gaccgagaaa tgggatctct acgttcgccg tatcgagttg gcgaccggtt attccgaact caccgacccg ataattcagc gggagcgctt cgaggcccag gcccgcgccg cggccgccgg tgacgacgag gcaatggcgc tggacgagga tttcctcgcc gccctcgagt acgggatgcc gccgtcgacc ggaaccggaa tggggatcga tcgattgatg atgacgttga ccggactgtc aatccgggag accgttttgt tcccgatagt caaacctcgt tag
Details for MythA.00612.a.B1.GE40759
| HARVESTED ON: | 1/31/2017 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 57kDa |
| OBSERVED MW: | 57kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMV TPPESVDPDL PEQFRIRQAK RADLLAQGRQ PYPVDVPRTH TLLEIRQAYP DLPVDARTGE IVGVTGRVVF ARNSGKLCFA TLQEGDGTQL QAMISLAEVG QEALDNWKTY VDIGDIVFVH GEVITSKRGE LSVLADSWQM AAKALRPLPV AHKEMSEESR VRQRYVDLIV RDQARKVARQ RIAVMRAIRS ALERRGFLEV ETPMLQTLAG GAAARPFVTH SNALDTELYL RIAPELFLKR CLVGGFERVF ELNRVFRNEG ADSTHSPEFV MLETYQAYGT YDDSAVVTRE LIQEVADEAI GTRQVPLPDG TVYDLDGEWE SIQMYPSLSE ALGEEITPDT PAETLWAIAD RLGLDIPRDR GYGHGKLVEE LWEHTVGAKL WAPTFVKDFP VETTPLTRSH RSIPGVTEKW DLYVRRIELA TGYSELTDPI IQRERFEAQA RAAAAGDDEA MALDEDFLAA LEYGMPPSTG TGMGIDRLMM TLTGLSIRET VL
Validated NT Sequence
acaaaacggt ctcccggatt gacagtccgg tcaacgtcat catcaatcga tcgatcccca ttccggttcc ggtcgacggc ggcatcccgt actcgagggc ggcgaggaaa tcctcgtcca gcgccattgc ctcgtcgtca ccggcggccg cggcgcgggc ctgggcctcg aagcgctccc gctgaattat cgggtcggtg agttcggaat aaccggtcgc caactcgata cggcgaacgt agagatccca tttctcggtc accccgggga tactgcggtg cgatctcgtc aacggcgtgg tctccaccgg gaaatccttg acgaaggtgg gcgcccacag tttcgcaccg acggtgtgct cccacagctc ctcgacgagc ttgccgtgcc cgtaaccacg atccctggga atatccaacc ccaaccggtc cgcgatcgcc cacaacgtct cggccggcgt gtcgggggtg atctcctcgc ccagcgcctc cgacagggag ggatacattt gaatcgactc ccattccccg tcgaggtcgt agaccgtgcc atcgggcaac ggcacttgtc gggtgccgat ggcctcgtcg gcgacttcct gaattaattc ccgggtcacc accgcggaat cgtcgtaggt gccgtacgcc tgataagtct ccaacatcac gaattccggg gaatgtgtgg aatccgcgcc ttcgtttcgg aacacccgat tcagttcgaa gacccgctcg aaaccgccga ccaggcagcg cttgaggaac aactccggcg caatccgcag ataaagctcg gtgtcgaggg catttgagtg ggtgacgaac gggcgggccg ccgcaccacc ggccaatgtc tgcaacatcg gcgtctcgac ttcgaggaat ccgcgccgct ccagcgccga acggatcgcg cgcatcaccg cgattcgttg ccgcgccacc ttccgggcct gatcgcgcac gatgaggtcc acgtaacgct gccggacccg ggattcctcc gacatctcct tgtgcgcgac cggcaatggc cgcagcgcct tggcggccat ttgccaggag tcggcgagca cagacagctc gccgcgtttc gaggtgatca cctcgccgtg cacgaagacg atgtcgccga tgtcgacgta ggtcttccag ttgtccagcg cttcctgccc cacctcggcg agactgatca tcgcctgtag ctgggtgccg tcgccttcct gcagggtcgc gaagcacagc ttgccggaat tccgggcgaa caccacccgg ccggtgacgc ctacgatctc gccggtgcgc gcgtcgacgg gcaggtcggg gtaggcctgc cggatctcga gcagggtgtg cgtccgcggc acgtcgaccg gatacggctg ccggccctga gcgagcaggt ccgcacgctt ggcctggcga atgcggaatt gttcgggcag gtcaggatca accgattcgg gtggagtcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT PPESVDPDLP EQFRIRQAKR ADLLAQGRQP YPVDVPRTHT LLEIRQAYPD LPVDARTGEI VGVTGRVVFA RNSGKLCFAT LQEGDGTQLQ AMISLAEVGQ EALDNWKTYV DIGDIVFVHG EVITSKRGEL SVLADSWQMA AKALRPLPVA HKEMSEESRV RQRYVDLIVR DQARKVARQR IAVMRAIRSA LERRGFLEVE TPMLQTLAGG AAARPFVTHS NALDTELYLR IAPELFLKRC LVGGFERVFE LNRVFRNEGA DSTHSPEFVM LETYQAYGTY DDSAVVTREL IQEVADEAIG TRQVPLPDGT VYDLDGEWES IQMYPSLSEA LGEEITPDTP AETLWAIADR LGLDIPRDRG YGHGKLVEEL WEHTVGAKLW APTFVKDFPV ETTPLTRSHR SIPGVTEKWD LYVRRIELAT GYSELTDPII QRERFEAQAR AAAAGDDEAM ALDEDFLAAL EYGMPPSTGT GMGIDRLMMT LTGLSIRETV LFPIVKPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactc cacccgaatc ggttgatcct gacctgcccg aacaattccg cattcgccag gccaagcgtg cggacctgct cgctcagggc cggcagccgt atccggtcga cgtgccgcgg acgcacaccc tgctcgagat ccggcaggcc taccccgacc tgcccgtcga cgcgcgcacc ggcgagatcg taggcgtcac cggccgggtg gtgttcgccc ggaattccgg caagctgtgc ttcgcgaccc tgcaggaagg cgacggcacc cagctacagg cgatgatcag tctcgccgag gtggggcagg aagcgctgga caactggaag acctacgtcg acatcggcga catcgtcttc gtgcacggcg aggtgatcac ctcgaaacgc ggcgagctgt ctgtgctcgc cgactcctgg caaatggccg ccaaggcgct gcggccattg ccggtcgcgc acaaggagat gtcggaggaa tcccgggtcc ggcagcgtta cgtggacctc atcgtgcgcg atcaggcccg gaaggtggcg cggcaacgaa tcgcggtgat gcgcgcgatc cgttcggcgc tggagcggcg cggattcctc gaagtcgaga cgccgatgtt gcagacattg gccggtggtg cggcggcccg cccgttcgtc acccactcaa atgccctcga caccgagctt tatctgcgga ttgcgccgga gttgttcctc aagcgctgcc tggtcggcgg tttcgagcgg gtcttcgaac tgaatcgggt gttccgaaac gaaggcgcgg attccacaca ttccccggaa ttcgtgatgt tggagactta tcaggcgtac ggcacctacg acgattccgc ggtggtgacc cgggaattaa ttcaggaagt cgccgacgag gccatcggca cccgacaagt gccgttgccc gatggcacgg tctacgacct cgacggggaa tgggagtcga ttcaaatgta tccctccctg tcggaggcgc tgggcgagga gatcaccccc gacacgccgg ccgagacgtt gtgggcgatc gcggaccggt tggggttgga tattcccagg gatcgtggtt acgggcacgg caagctcgtc gaggagctgt gggagcacac cgtcggtgcg aaactgtggg cgcccacctt cgtcaaggat ttcccggtgg agaccacgcc gttgacgaga tcgcaccgca gtatccccgg ggtgaccgag aaatgggatc tctacgttcg ccgtatcgag ttggcgaccg gttattccga actcaccgac ccgataattc agcgggagcg cttcgaggcc caggcccgcg ccgcggccgc cggtgacgac gaggcaatgg cgctggacga ggatttcctc gccgccctcg agtacgggat gccgccgtcg accggaaccg gaatggggat cgatcgattg atgatgacgt tgaccggact gtcaatccgg gagaccgttt tgttcccgat agtcaaacct cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MythA.00612.a.B1.PW38257
| PURIFICATION DATe: | 4/24/2017 |
| CONCENTRATION: | 33.5mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TPPESVDPDL PEQFRIRQAK RADLLAQGRQ PYPVDVPRTH TLLEIRQAYP DLPVDARTGE IVGVTGRVVF ARNSGKLCFA TLQEGDGTQL QAMISLAEVG QEALDNWKTY VDIGDIVFVH GEVITSKRGE LSVLADSWQM AAKALRPLPV AHKEMSEESR VRQRYVDLIV RDQARKVARQ RIAVMRAIRS ALERRGFLEV ETPMLQTLAG GAAARPFVTH SNALDTELYL RIAPELFLKR CLVGGFERVF ELNRVFRNEG ADSTHSPEFV MLETYQAYGT YDDSAVVTRE LIQEVADEAI GTRQVPLPDG TVYDLDGEWE SIQMYPSLSE ALGEEITPDT PAETLWAIAD RLGLDIPRDR GYGHGKLVEE LWEHTVGAKL WAPTFVKDFP VETTPLTRSH RSIPGVTEKW DLYVRRIELA TGYSELTDPI IQRERFEAQA RAAAAGDDEA MALDEDFLAA LEYGMPPSTG TGMGIDRLMM TLTGLSIRET VL
Validated NT Sequence
acaaaacggt ctcccggatt gacagtccgg tcaacgtcat catcaatcga tcgatcccca ttccggttcc ggtcgacggc ggcatcccgt actcgagggc ggcgaggaaa tcctcgtcca gcgccattgc ctcgtcgtca ccggcggccg cggcgcgggc ctgggcctcg aagcgctccc gctgaattat cgggtcggtg agttcggaat aaccggtcgc caactcgata cggcgaacgt agagatccca tttctcggtc accccgggga tactgcggtg cgatctcgtc aacggcgtgg tctccaccgg gaaatccttg acgaaggtgg gcgcccacag tttcgcaccg acggtgtgct cccacagctc ctcgacgagc ttgccgtgcc cgtaaccacg atccctggga atatccaacc ccaaccggtc cgcgatcgcc cacaacgtct cggccggcgt gtcgggggtg atctcctcgc ccagcgcctc cgacagggag ggatacattt gaatcgactc ccattccccg tcgaggtcgt agaccgtgcc atcgggcaac ggcacttgtc gggtgccgat ggcctcgtcg gcgacttcct gaattaattc ccgggtcacc accgcggaat cgtcgtaggt gccgtacgcc tgataagtct ccaacatcac gaattccggg gaatgtgtgg aatccgcgcc ttcgtttcgg aacacccgat tcagttcgaa gacccgctcg aaaccgccga ccaggcagcg cttgaggaac aactccggcg caatccgcag ataaagctcg gtgtcgaggg catttgagtg ggtgacgaac gggcgggccg ccgcaccacc ggccaatgtc tgcaacatcg gcgtctcgac ttcgaggaat ccgcgccgct ccagcgccga acggatcgcg cgcatcaccg cgattcgttg ccgcgccacc ttccgggcct gatcgcgcac gatgaggtcc acgtaacgct gccggacccg ggattcctcc gacatctcct tgtgcgcgac cggcaatggc cgcagcgcct tggcggccat ttgccaggag tcggcgagca cagacagctc gccgcgtttc gaggtgatca cctcgccgtg cacgaagacg atgtcgccga tgtcgacgta ggtcttccag ttgtccagcg cttcctgccc cacctcggcg agactgatca tcgcctgtag ctgggtgccg tcgccttcct gcagggtcgc gaagcacagc ttgccggaat tccgggcgaa caccacccgg ccggtgacgc ctacgatctc gccggtgcgc gcgtcgacgg gcaggtcggg gtaggcctgc cggatctcga gcagggtgtg cgtccgcggc acgtcgaccg gatacggctg ccggccctga gcgagcaggt ccgcacgctt ggcctggcga atgcggaatt gttcgggcag gtcaggatca accgattcgg gtggagtcac catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMT PPESVDPDLP EQFRIRQAKR ADLLAQGRQP YPVDVPRTHT LLEIRQAYPD LPVDARTGEI VGVTGRVVFA RNSGKLCFAT LQEGDGTQLQ AMISLAEVGQ EALDNWKTYV DIGDIVFVHG EVITSKRGEL SVLADSWQMA AKALRPLPVA HKEMSEESRV RQRYVDLIVR DQARKVARQR IAVMRAIRSA LERRGFLEVE TPMLQTLAGG AAARPFVTHS NALDTELYLR IAPELFLKRC LVGGFERVFE LNRVFRNEGA DSTHSPEFVM LETYQAYGTY DDSAVVTREL IQEVADEAIG TRQVPLPDGT VYDLDGEWES IQMYPSLSEA LGEEITPDTP AETLWAIADR LGLDIPRDRG YGHGKLVEEL WEHTVGAKLW APTFVKDFPV ETTPLTRSHR SIPGVTEKWD LYVRRIELAT GYSELTDPII QRERFEAQAR AAAAGDDEAM ALDEDFLAAL EYGMPPSTGT GMGIDRLMMT LTGLSIRETV LFPIVKPR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACTC CACCCGAATC GGTTGATCCT GACCTGCCCG AACAATTCCG CATTCGCCAG GCCAAGCGTG CGGACCTGCT CGCTCAGGGC CGGCAGCCGT ATCCGGTCGA CGTGCCGCGG ACGCACACCC TGCTCGAGAT CCGGCAGGCC TACCCCGACC TGCCCGTCGA CGCGCGCACC GGCGAGATCG TAGGCGTCAC CGGCCGGGTG GTGTTCGCCC GGAATTCCGG CAAGCTGTGC TTCGCGACCC TGCAGGAAGG CGACGGCACC CAGCTACAGG CGATGATCAG TCTCGCCGAG GTGGGGCAGG AAGCGCTGGA CAACTGGAAG ACCTACGTCG ACATCGGCGA CATCGTCTTC GTGCACGGCG AGGTGATCAC CTCGAAACGC GGCGAGCTGT CTGTGCTCGC CGACTCCTGG CAAATGGCCG CCAAGGCGCT GCGGCCATTG CCGGTCGCGC ACAAGGAGAT GTCGGAGGAA TCCCGGGTCC GGCAGCGTTA CGTGGACCTC ATCGTGCGCG ATCAGGCCCG GAAGGTGGCG CGGCAACGAA TCGCGGTGAT GCGCGCGATC CGTTCGGCGC TGGAGCGGCG CGGATTCCTC GAAGTCGAGA CGCCGATGTT GCAGACATTG GCCGGTGGTG CGGCGGCCCG CCCGTTCGTC ACCCACTCAA ATGCCCTCGA CACCGAGCTT TATCTGCGGA TTGCGCCGGA GTTGTTCCTC AAGCGCTGCC TGGTCGGCGG TTTCGAGCGG GTCTTCGAAC TGAATCGGGT GTTCCGAAAC GAAGGCGCGG ATTCCACACA TTCCCCGGAA TTCGTGATGT TGGAGACTTA TCAGGCGTAC GGCACCTACG ACGATTCCGC GGTGGTGACC CGGGAATTAA TTCAGGAAGT CGCCGACGAG GCCATCGGCA CCCGACAAGT GCCGTTGCCC GATGGCACGG TCTACGACCT CGACGGGGAA TGGGAGTCGA TTCAAATGTA TCCCTCCCTG TCGGAGGCGC TGGGCGAGGA GATCACCCCC GACACGCCGG CCGAGACGTT GTGGGCGATC GCGGACCGGT TGGGGTTGGA TATTCCCAGG GATCGTGGTT ACGGGCACGG CAAGCTCGTC GAGGAGCTGT GGGAGCACAC CGTCGGTGCG AAACTGTGGG CGCCCACCTT CGTCAAGGAT TTCCCGGTGG AGACCACGCC GTTGACGAGA TCGCACCGCA GTATCCCCGG GGTGACCGAG AAATGGGATC TCTACGTTCG CCGTATCGAG TTGGCGACCG GTTATTCCGA ACTCACCGAC CCGATAATTC AGCGGGAGCG CTTCGAGGCC CAGGCCCGCG CCGCGGCCGC CGGTGACGAC GAGGCAATGG CGCTGGACGA GGATTTCCTC GCCGCCCTCG AGTACGGGAT GCCGCCGTCG ACCGGAACCG GAATGGGGAT CGATCGATTG ATGATGACGT TGACCGGACT GTCAATCCGG GAGACCGTTT TGTTCCCGAT AGTCAAACCT CGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT