SSGCID: Target Page

MythA.00572.a

ABC transporter ATP-binding protein (A0R2D7 homolog)

CENTER ID:	MythA.00572.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00572.a.A1.GE28742	Full length( MythA.00572.a )		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1478
RefSeq:	WP_003925001.1
UniProt:	G7CET4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRCSTRSWP GRPNSCPTKE GHMTTTSPRF DAEPAVEIHG LKKSFGRTRA VDGLDLRVER GSVAGFLGPN GSGKSTTIRV LLGLLRADGG TARLLGGDPW HDAVELHRRL AYVPGDVTLW PNLTGGQAID FLAGLRDNFD TKRRDELIER FELDPDKKAR TYSKGNRQKV ALVAAFSTNA EIYILDEPTS GLDPLMDKAF QMCVREAADN GAAVLLSSHI LAEVERLCDS VTIIRSGRTV RAGRLDELRH LMRTTVKVRT RAPIDALRTA PYIHEFSTDD GLHTFSVDRT DLDRTMTELT TLGIEELTVT PASLEDLFLR EYQEVPR

NT Sequence

ATTCGACGAT GTTCGACGCG TTCGTGGCCC GGGAGACCCA ACAGTTGTCC GACCAAGGAG GGGCACATGA CGACGACGTC GCCGCGGTTT GACGCCGAAC CAGCCGTCGA AATCCACGGA CTGAAGAAGA GTTTCGGCCG CACCAGGGCG GTCGACGGCC TGGACCTACG GGTCGAGCGG GGCAGCGTCG CCGGATTCCT CGGCCCCAAC GGCTCGGGCA AGTCGACCAC CATCCGGGTG CTGCTGGGTC TGCTGCGCGC CGACGGCGGC ACCGCCCGAC TGCTCGGCGG CGACCCGTGG CACGACGCGG TCGAACTGCA CCGCCGCCTG GCATACGTGC CCGGGGATGT GACGCTGTGG CCCAATCTCA CCGGCGGGCA GGCCATCGAC TTCCTGGCCG GCCTGCGCGA CAACTTCGAC ACCAAACGCC GGGACGAACT GATCGAACGG TTCGAACTCG ACCCGGACAA GAAGGCCCGC ACCTACTCCA AGGGCAACCG GCAGAAGGTG GCGCTGGTCG CGGCGTTCAG CACCAACGCC GAGATCTACA TTCTCGACGA ACCGACCTCC GGTCTGGACC CGTTGATGGA CAAGGCGTTC CAAATGTGTG TGCGGGAGGC CGCCGACAAC GGTGCCGCGG TGCTGCTGTC CAGTCACATC CTCGCCGAGG TGGAGCGGCT GTGTGACTCG GTGACCATCA TCCGGTCCGG CCGGACGGTG CGCGCCGGCC GGCTCGACGA ATTGCGGCAC CTGATGCGCA CCACCGTCAA GGTGCGCACC CGTGCCCCGA TCGACGCGCT GCGTACGGCC CCCTACATTC ACGAGTTCAG CACCGACGAC GGTCTGCACA CCTTCTCGGT GGACCGCACC GATCTGGACC GCACGATGAC CGAGCTGACG ACGCTCGGCA TCGAGGAGCT GACCGTGACC CCGGCCTCGC TCGAGGACCT GTTCCTGCGC GAGTACCAGG AGGTGCCGCG A

Details for MythA.00572.a.A1.GE28742

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MGTLEAQTQG PGSMIRRCST RSWPGRPNSC PTKEGHMTTT SPRFDAEPAV EIHGLKKSFG RTRAVDGLDL RVERGSVAGF LGPNGSGKST TIRVLLGLLR ADGGTARLLG GDPWHDAVEL HRRLAYVPGD VTLWPNLTGG QAIDFLAGLR DNFDTKRRDE LIERFELDPD KKARTYSKGN RQKVALVAAF STNAEIYILD EPTSGLDPLM DKAFQMCVRE AADNGAAVLL SSHILAXVER LCDSVTIIRS GRTVRAGRLD ELRHLMRTTV KVRTRAPIDA LRTAPYIHEF STDDGLHTFS VDRTDLDRTM TELTTLGIEE LTVTPASLED LFLRE

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgattcg acgatgttcg acgcgttcgt ggcccgggag acccaacagt tgtccgacca aggaggggca catgacgacg acgtcgccgc ggtttgacgc cgaaccagcc gtcgaaatcc acggactgaa gaagagtttc ggccgcacca gggcggtcga cggcctggac ctacgggtcg agcggggcag cgtcgccgga ttcctcggcc ccaacggctc gggcaagtcg accaccatcc gggtgctgct gggtctgctg cgcgccgacg gcggcaccgc ccgactgctc ggcggcgacc cgtggcacga cgcggtcgaa ctgcaccgcc gcctggcata cgtgcccggg gatgtgacgc tgtggcccaa tctcaccggc gggcaggcca tcgacttcct ggccggcctg cgcgacaact tcgacaccaa acgccgggac gaactgatcg aacggttcga actcgacccg gacaagaagg cccgcaccta ctccaagggc aaccggcaga aggtggcgct ggtcgcggcg ttcagcacca acgccgagat ctacattctc gacgaaccga cctccggtct ggacccgttg atggacaagg cgttccaaat gtgtgtgcgg gaggccgccg acaacggtgc cgcggtgctg ctgtccagtc acatcctcgc cgangtggag cggctgtgtg actcggtgac catcatccgg tccggccgga cggtgcgcgc cggccggctc gacgaattgc ggcacctgat gcgcaccacc gtcaaggtgc gcacccgtgc cccgatcgac gcgctgcgta cggcccccta cattcacgag ttcagcaccg acgacggtct gcacaccttc tcggtggacc gcaccgatct ggaccgcacg atgaccgagc tgacgacgct cggcatcgag gagctgaccg tgaccccggc ctcgctcgag gacctgttcc tgcgcgagt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIRRCSTRS WPGRPNSCPT KEGHMTTTSP RFDAEPAVEI HGLKKSFGRT RAVDGLDLRV ERGSVAGFLG PNGSGKSTTI RVLLGLLRAD GGTARLLGGD PWHDAVELHR RLAYVPGDVT LWPNLTGGQA IDFLAGLRDN FDTKRRDELI ERFELDPDKK ARTYSKGNRQ KVALVAAFST NAEIYILDEP TSGLDPLMDK AFQMCVREAA DNGAAVLLSS HILAEVERLC DSVTIIRSGR TVRAGRLDEL RHLMRTTVKV RTRAPIDALR TAPYIHEFST DDGLHTFSVD RTDLDRTMTE LTTLGIEELT VTPASLEDLF LREYQEVPR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcgacga tgttcgacgc gttcgtggcc cgggagaccc aacagttgtc cgaccaagga ggggcacatg acgacgacgt cgccgcggtt tgacgccgaa ccagccgtcg aaatccacgg actgaagaag agtttcggcc gcaccagggc ggtcgacggc ctggacctac gggtcgagcg gggcagcgtc gccggattcc tcggccccaa cggctcgggc aagtcgacca ccatccgggt gctgctgggt ctgctgcgcg ccgacggcgg caccgcccga ctgctcggcg gcgacccgtg gcacgacgcg gtcgaactgc accgccgcct ggcatacgtg cccggggatg tgacgctgtg gcccaatctc accggcgggc aggccatcga cttcctggcc ggcctgcgcg acaacttcga caccaaacgc cgggacgaac tgatcgaacg gttcgaactc gacccggaca agaaggcccg cacctactcc aagggcaacc ggcagaaggt ggcgctggtc gcggcgttca gcaccaacgc cgagatctac attctcgacg aaccgacctc cggtctggac ccgttgatgg acaaggcgtt ccaaatgtgt gtgcgggagg ccgccgacaa cggtgccgcg gtgctgctgt ccagtcacat cctcgccgag gtggagcggc tgtgtgactc ggtgaccatc atccggtccg gccggacggt gcgcgccggc cggctcgacg aattgcggca cctgatgcgc accaccgtca aggtgcgcac ccgtgccccg atcgacgcgc tgcgtacggc cccctacatt cacgagttca gcaccgacga cggtctgcac accttctcgg tggaccgcac cgatctggac cgcacgatga ccgagctgac gacgctcggc atcgaggagc tgaccgtgac cccggcctcg ctcgaggacc tgttcctgcg cgagtaccag gaggtgccgc gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa