SSGCID: Target Page

MythA.00556.a

Succinyl-CoA ligase [ADP-forming] subunit alpha (A0R3M3 homolog)

CENTER ID:	MythA.00556.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00556.a.A1.GE28741	Full length( MythA.00556.a )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2199
RefSeq:	WP_003925725.1
UniProt:	G7CJ59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIFLNKNSK VIVQGITGGE GTKHTALMLK AGTQIVGGVN ARKAGTTVSH TDKDGKDVEL PVFGTVEEAM KETGADVSVV FVPPKFAKDA IIEAIDAEIA LLVVITEGIP VQDTAYAWAY NVKRGQKTRI IGPNCPGIIT PGEALAGITP ANITGPGPIG LVSKSGTLTY QMMFELSDFG FSTAIGIGGD PIIGTTHIDA IEAFEKDPDT KLIVMIGEIG GDAEERAAEY IKANVSKPVV GYVAGFTAPE GKTMGHAGAI VSGSSGTAQA KKEALEAAGV KVGKTPSETA RLAREILQSL

NT Sequence

ATGTCTATCT TCCTGAACAA GAACTCCAAG GTCATCGTCC AGGGCATCAC CGGCGGTGAG GGCACCAAGC ACACCGCGCT GATGCTCAAG GCCGGCACCC AGATCGTCGG CGGCGTCAAC GCCCGCAAGG CCGGCACCAC CGTGTCGCAC ACCGACAAGG ACGGCAAGGA CGTCGAACTC CCGGTGTTCG GCACCGTCGA AGAGGCGATG AAGGAGACCG GCGCGGACGT GTCGGTGGTG TTCGTGCCGC CGAAGTTCGC CAAGGACGCG ATCATCGAGG CCATCGACGC CGAGATCGCG CTGCTGGTCG TCATCACCGA GGGCATCCCG GTGCAGGACA CCGCCTATGC GTGGGCCTAC AACGTCAAGA GGGGGCAGAA GACCCGCATC ATCGGGCCGA ACTGCCCGGG CATCATCACC CCCGGTGAGG CGCTGGCCGG TATCACCCCG GCCAACATCA CCGGGCCGGG ACCGATCGGG CTGGTCTCGA AGTCCGGCAC CCTGACCTAC CAGATGATGT TCGAGCTCAG CGACTTCGGC TTCTCCACCG CGATCGGCAT CGGTGGTGAC CCGATCATCG GCACCACCCA CATCGACGCG ATCGAGGCGT TCGAGAAGGA TCCGGACACC AAGCTGATCG TGATGATCGG CGAGATCGGC GGCGACGCCG AGGAGCGTGC CGCCGAGTAC ATCAAGGCCA ACGTCTCCAA GCCGGTCGTC GGCTATGTCG CGGGCTTCAC CGCCCCGGAG GGCAAGACGA TGGGCCACGC CGGCGCCATC GTGTCCGGGT CGTCGGGCAC CGCGCAGGCC AAGAAGGAGG CCCTGGAGGC GGCCGGCGTG AAGGTCGGCA AGACCCCGTC GGAGACCGCG CGCCTGGCCC GCGAGATCCT GCAGAGCCTG

Details for MythA.00556.a.A1.GE28741

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	33kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSIFLNKNS KVIVQGITGG EGTKHTALML KAGTQIVGGV NARKAGTTVS HTDKDGKDVE LPVFGTVEEA MKETGADVSV VFVPPKFAKD AIIEAIDAEI ALLVVITEGI PVQDTAYAWA YNVKRGQKTR IIGPNCPGII TPGEALAGIT PANITGPGPI GLVSKSGTLT YQMMFELSDF GFSTAIGIGG DPIIGTTHID AIEAFEKDPD TKLIVMIGEI GGDAEERAAE YIKANVSKPV VGYVAGFTAP EGKTMGHAGA IVSGSSGTAQ AKKEALEAAG VKVGKTPSET ARLAREILQS L

Validated NT Sequence

ttattacagg ctctgcagga tctcgcgggc caggcgcgcg gtctccgacg gggtcttgcc gaccttcacg ccggccgcct ccagggcctc cttcttggcc tgcgcggtgc ccgacgaccc ggacacgatg gcgccggcgt ggcccatcgt cttgccctcc ggggcggtga agcccgcgac atagccgacg accggcttgg agacgttggc cttgatgtac tcggcggcac gctcctcggc gtcgccgccg atctcgccga tcatcacgat cagcttggtg tccggatcct tctcgaacgc ctcgatcgcg tcgatgtggg tggtgccgat gatcgggtca ccaccgatgc cgatcgcggt ggagaagccg aagtcgctga gctcgaacat catctggtag gtcagggtgc cggacttcga gaccagcccg atcggtcccg gcccggtgat gttggccggg gtgataccgg ccagcgcctc accgggggtg atgatgcccg ggcagttcgg cccgatgatg cgggtcttct gccccctctt gacgttgtag gcccacgcat aggcggtgtc ctgcaccggg atgccctcgg tgatgacgac cagcagcgcg atctcggcgt cgatggcctc gatgatcgcg tccttggcga acttcggcgg cacgaacacc accgacacgt ccgcgccggt ctccttcatc gcctcttcga cggtgccgaa caccgggagt tcgacgtcct tgccgtcctt gtcggtgtgc gacacggtgg tgccggcctt gcgggcgttg acgccgccga cgatctgggt gccggccttg agcatcagcg cggtgtgctt ggtgccctca ccgccggtga tgccctggac gatgaccttg gagttcttgt tcaggaagat agacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctatcttc ctgaacaaga actccaaggt catcgtccag ggcatcaccg gcggtgaggg caccaagcac accgcgctga tgctcaaggc cggcacccag atcgtcggcg gcgtcaacgc ccgcaaggcc ggcaccaccg tgtcgcacac cgacaaggac ggcaaggacg tcgaactccc ggtgttcggc accgtcgaag aggcgatgaa ggagaccggc gcggacgtgt cggtggtgtt cgtgccgccg aagttcgcca aggacgcgat catcgaggcc atcgacgccg agatcgcgct gctggtcgtc atcaccgagg gcatcccggt gcaggacacc gcctatgcgt gggcctacaa cgtcaagagg gggcagaaga cccgcatcat cgggccgaac tgcccgggca tcatcacccc cggtgaggcg ctggccggta tcaccccggc caacatcacc gggccgggac cgatcgggct ggtctcgaag tccggcaccc tgacctacca gatgatgttc gagctcagcg acttcggctt ctccaccgcg atcggcatcg gtggtgaccc gatcatcggc accacccaca tcgacgcgat cgaggcgttc gagaaggatc cggacaccaa gctgatcgtg atgatcggcg agatcggcgg cgacgccgag gagcgtgccg ccgagtacat caaggccaac gtctccaagc cggtcgtcgg ctatgtcgcg ggcttcaccg ccccggaggg caagacgatg ggccacgccg gcgccatcgt gtccgggtcg tcgggcaccg cgcaggccaa gaaggaggcc ctggaggcgg ccggcgtgaa ggtcggcaag accccgtcgg agaccgcgcg cctggcccgc gagatcctgc agagcctgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa