MythA.00554.a

3-Hydroxyacyl-CoA dehydrogenase (A0R2P1 homolog)

CENTER ID:	MythA.00554.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00554.a.A1.GE28739	Full length( MythA.00554.a )		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.3200
RefSeq:	WP_003926732.1
UniProt:	G7CI08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAGLRRHRR VCDAGAVVRR GGERGPRGRT QHLLPRGRPP AARTGPAILP QRAVGADAAA RSRRRHRRRA AGLAVTQGKG RNSSGDQXXX XXXXXXXXXX XXXXXGLATT KRLLDRGAQV VVLDLKGEDA VKALGDRARF VETNVTDEAA VAKALDVAEE MGPVRINVNC AGIGNAIKTL SKNGPFPLDE FTKVIQVNLI GTFNVLRLAA ERIAKTEPLK GEERGVIINT ASVAAFEGQI GQAAYSASKG GVVGMTLPIA RDLARELIRV VTIAPGLFKT PLLGSLPEEA QKSLGQQVPH PARLGDPDEY GALAVHIVEN PMLNGEVIRL DGAIRMAPR

NT Sequence

CTGGGCGCAG GTCTTCGCCG GCACCGACGC GTGTGTGACG CCGGTGCTGT CGTTCGCCGA GGTGGAGAGC GAGGCCCACG TGGTCGAACG CAACACCTTC TACCGCGAGG GCGACCACCT GCTGCCCGCA CCGGCCCCGC GATTCTCCCG CAGCGTGCCG TCGGCGCCGA CGCCGCCGCG CGATCCCGGC GCCGACACCG ACGCCGTGCT GCAGGACTGG CAGTAACACA AGGGAAGGGA AGAAATAGCA GTGGAGATCA AAGNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCCT GGCGACCACC AAGCGTCTGC TCGATCGGGG CGCTCAGGTC GTGGTGCTCG ACCTCAAGGG TGAGGACGCC GTCAAGGCGC TCGGTGACCG TGCCCGCTTC GTCGAAACCA ACGTCACGGA CGAGGCCGCC GTCGCCAAGG CGCTCGACGT GGCCGAGGAG ATGGGCCCGG TGCGCATCAA CGTCAACTGC GCCGGCATCG GCAACGCGAT CAAGACGCTG AGCAAGAACG GCCCGTTCCC GCTCGACGAG TTCACCAAGG TCATCCAGGT GAACCTGATC GGCACCTTCA ACGTGCTGCG GTTGGCCGCC GAGCGGATCG CCAAGACCGA GCCGCTCAAG GGCGAGGAGC GCGGCGTCAT CATCAACACC GCCTCGGTCG CGGCGTTCGA GGGCCAGATC GGTCAGGCCG CGTACTCGGC GTCCAAGGGC GGTGTCGTCG GCATGACCCT GCCGATCGCC CGTGACCTGG CCCGTGAGCT GATCCGCGTG GTGACCATCG CGCCGGGTCT GTTCAAGACC CCGCTGCTGG GCTCGCTGCC CGAGGAGGCG CAGAAGTCGC TCGGCCAGCA GGTGCCGCAT CCGGCGCGGC TCGGCGATCC CGACGAGTAC GGTGCGCTGG CCGTGCACAT CGTCGAGAAC CCGATGCTCA ACGGTGAGGT GATCCGCCTC GACGGCGCAA TCCGTATGGC ACCGCGG

Details for MythA.00554.a.A1.GE28739

HARVESTED ON:	data unavailable
SEQUENCED ON:	5/19/2010
EXPECTED MW:	38kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	83
PERCENT COVERAGE:	39

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLGAGLRRH RRVCDAGAVV RRGGERGPRG RTQHLLPRGR PPAARTGPAI LPQRAVGADA AARSRRRHRR RAAGLAVTQG KGRNSSGDQR LGRSRHRRRI RSGPGDHQAS ARSGRSGRGA RPQG

Validated NT Sequence

ttattaccgc ggtgccatac ggattgcgcc gtcgaggcgg atcacctcac cgttgagcat cgggttctcg acgatgtgca cggccagcgc accgtactcg tcgggatcgc cgagccgcgc cggatgcggc acctgctggc cgagcgactt ctgcgcctcc tcgggcagcg agcccagcag cggggtcttg aacagacccg gcgcgatggt caccacgcgg atcagctcac gggccaggtc acgggcgatc ggcagggtca tgccgacgac accgcccttg gacgccgagt acgcggcctg accgatctgg ccctcgaacg ccgcgaccga ggcggtgttg atgatgacgc cgcgctcctc gcccttgagc ggctcggtct tggcgatccg ctcggcggcc aaccgcagca cgttgaaggt gccgatcagg ttcacctgga tgaccttggt gaactcgtcg agcgggaacg ggccgttctt gctcagcgtc ttgatcgcgt tgccgatgcc ggcgcagttg acgttgatgc gcaccgggcc catctcctcg gccacgtcga gcgccttggc gacggcggcc tcgtccgtga cgttggtttc gacgaagcgg gcacggtcac cgagcgcctt gacggcgtcc tcacccttga ggtcgagcac cacgacctga gcgccccgat cgagcagacg cttggtggtc gccaggccca gaccggatgc gccgccggtg acgactgcga ccgagtcttt gatctccact gctatttctt cccttccctt gtgttactgc cagtcctgca gcacggcgtc ggtgtcggcg ccgggatcgc gcggcggcgt cggcgccgac ggcacgctgc gggagaatcg cggggccggt gcgggcagca ggtggtcgcc ctcgcggtag aaggtgttgc gttcgaccac gtgggcctcg ctctccacct cggcgaacga cagcaccggc gtcacacacg cgtcggtgcc ggcgaagacc tgcgcccagc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLGAGLRRH RRVCDAGAVV RRGGERGPRG RTQHLLPRGR PPAARTGPAI LPQRAVGADA AARSRRRHRR RAAGLAVTQG KGRNSSGDQX XXXXXXXXXX XXXXXXXGLA TTKRLLDRGA QVVVLDLKGE DAVKALGDRA RFVETNVTDE AAVAKALDVA EEMGPVRINV NCAGIGNAIK TLSKNGPFPL DEFTKVIQVN LIGTFNVLRL AAERIAKTEP LKGEERGVII NTASVAAFEG QIGQAAYSAS KGGVVGMTLP IARDLARELI RVVTIAPGLF KTPLLGSLPE EAQKSLGQQV PHPARLGDPD EYGALAVHIV ENPMLNGEVI RLDGAIRMAP R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgggcgca ggtcttcgcc ggcaccgacg cgtgtgtgac gccggtgctg tcgttcgccg aggtggagag cgaggcccac gtggtcgaac gcaacacctt ctaccgcgag ggcgaccacc tgctgcccgc accggccccg cgattctccc gcagcgtgcc gtcggcgccg acgccgccgc gcgatcccgg cgccgacacc gacgccgtgc tgcaggactg gcagtaacac aagggaaggg aagaaatagc agtggagatc aaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggcc tggcgaccac caagcgtctg ctcgatcggg gcgctcaggt cgtggtgctc gacctcaagg gtgaggacgc cgtcaaggcg ctcggtgacc gtgcccgctt cgtcgaaacc aacgtcacgg acgaggccgc cgtcgccaag gcgctcgacg tggccgagga gatgggcccg gtgcgcatca acgtcaactg cgccggcatc ggcaacgcga tcaagacgct gagcaagaac ggcccgttcc cgctcgacga gttcaccaag gtcatccagg tgaacctgat cggcaccttc aacgtgctgc ggttggccgc cgagcggatc gccaagaccg agccgctcaa gggcgaggag cgcggcgtca tcatcaacac cgcctcggtc gcggcgttcg agggccagat cggtcaggcc gcgtactcgg cgtccaaggg cggtgtcgtc ggcatgaccc tgccgatcgc ccgtgacctg gcccgtgagc tgatccgcgt ggtgaccatc gcgccgggtc tgttcaagac cccgctgctg ggctcgctgc ccgaggaggc gcagaagtcg ctcggccagc aggtgccgca tccggcgcgg ctcggcgatc ccgacgagta cggtgcgctg gccgtgcaca tcgtcgagaa cccgatgctc aacggtgagg tgatccgcct cgacggcgca atccgtatgg caccgcggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa