SSGCID: Target Page

MythA.00392.a

Cell division ATP-binding protein FtsE (A0QU61 homolog)

CENTER ID:	MythA.00392.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00392.a.A2.GE29583	potential soluble domain		1	176

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2839
RefSeq:	WP_003926371.1
UniProt:	G7CGZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATITYRNAS CIYEGSDTFA VNSLNLEIED GEFVVLVGPS GSGKSTALRM LAGLEDIDDG DIEIGGRNMR GVPSKDRDIA MVFQNYALYP NKTVAENMGF ALKLRGVAAS ERRRKVEEAA KLLDLTEFLD RKPAKLSGGQ RQRVAMGRAI VREPQVFCMD EPLSNLDAKL RVQTRTQIAA LQRRLGPPPX XXXXXXXRSR R

NT Sequence

ATGGCGACAA TCACCTACCG CAACGCGTCG TGCATCTACG AAGGGTCCGA CACGTTCGCG GTGAACTCGC TCAACCTCGA GATCGAGGAC GGTGAGTTCG TGGTGCTGGT CGGGCCGTCC GGGTCGGGGA AGAGCACCGC ACTGCGGATG CTGGCCGGGC TGGAGGACAT CGACGACGGC GACATCGAGA TCGGTGGCCG CAACATGCGC GGGGTGCCGT CGAAGGACCG CGACATCGCG ATGGTGTTCC AGAACTACGC GCTGTATCCG AACAAGACCG TCGCGGAGAA CATGGGATTC GCGCTGAAAC TGCGCGGGGT GGCGGCCTCC GAGCGGCGCC GCAAGGTCGA GGAGGCCGCC AAACTGCTCG ACCTCACCGA GTTCCTGGAC CGCAAGCCGG CCAAGCTGTC CGGTGGGCAG CGCCAACGGG TGGCGATGGG CCGGGCGATC GTGCGGGAGC CGCAGGTGTT CTGCATGGAC GAACCGCTGT CGAACCTCGA TGCCAAACTG CGGGTGCAGA CCCGCACCCA GATCGCCGCG CTGCAGCGTC GGCTCGGCCC CCCCCCCNNN NNNNNNNNNN NNNNNNNNNN NAGGTCGAGG CGA

Details for MythA.00392.a.A2.GE29583

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	21kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMATITYRNA SCIYEGSDTF AVNSLNLEIE DGEFVVLVGP SGSGKSTALR MLAGLEDIDD GDIEIGGRNM RGVPSKDRDI AMVFQNYALY PNKTVAENMG FALKLRGVAA SERRRKVEEA AKLLDLTEFL DRKPAKLSGG QRQRVAMGRA IVREPQVFCM DEPLSNLDAK LRVQTRT

Validated NT Sequence

tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgac aatcacctac cgcaacgcgt cgtgcatcta cgaagggtcc gacacgttcg cggtgaactc gctcaacctc gagatcgagg acggtgagtt cgtggtgctg gtcgggccgt ccgggtcggg gaagagcacc gcactgcgga tgctggccgg gctggaggac atcgacgacg gcgacatcga gatcggtggc cgcaacatgc gcggggtgcc gtcgaaggac cgcgacatcg cgatggtgtt ccagaactac gcgctgtatc cgaacaagac cgtcgcggag aacatgggat tcgcgctgaa actgcgcggg gtggcggcct ccgagcggcg ccgcaaggtc gaggaggccg ccaaactgct cgacctcacc gagttcctgg accgcaagcc ggccaagctg tccggtgggc agcgccaacg ggtggcgatg ggccgggcga tcgtgcggga gccgcaggtg ttctgcatgg acgaaccgct gtcgaacctc gatgccaaac tgcgggtgca gacccgcacc taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgantt ggctgctgnc accgnnnnnn nntttnnnnn nattntgtnn annnnnnnnn nnnngnnnnn ntnnnnnnnn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgacaatc acctaccgca acgcgtcgtg catctacgaa gggtccgaca cgttcgcggt gaactcgctc aacctcgaga tcgaggacgg tgagttcgtg gtgctggtcg ggccgtccgg gtcggggaag agcaccgcac tgcggatgct ggccgggctg gaggacatcg acgacggcga catcgagatc ggtggccgca acatgcgcgg ggtgccgtcg aaggaccgcg acatcgcgat ggtgttccag aactacgcgc tgtatccgaa caagaccgtc gcggagaaca tgggattcgc gctgaaactg cgcggggtgg cggcctccga gcggcgccgc aaggtcgagg aggccgccaa actgctcgac ctcaccgagt tcctggaccg caagccggcc aagctgtccg gtgggcagcg ccaacgggtg gcgatgggcc gggcgatcgt gcgggagccg caggtgttct gcatggacga accgctgtcg aacctcgatg ccaaactgcg ggtgcagacc cgcaccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa