SSGCID: Target Page

MythA.00328.a

50S ribosomal protein L1 (A0QS46 homolog)

CENTER ID:	MythA.00328.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00328.a.A1.GE28726	Full length( MythA.00328.a )		1	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2467
RefSeq:	WP_003925994.1
UniProt:	G7CKS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDGHMSKKSK AYREAAAKVD RDRLYHPLEA AKLAKETSSK KQDATVEVAI RLGVDPRKAD QMVRGTVNLP HGTGKTARVA VFAVGEKAEE AKAAGADIVG SDDLIEQIQG GMLDFDAAIA TPDQMAKVGR IARILGPRGL MPNPKTGTVT TDIAKAVQDI KGGKINFRVD KAANLHLVIG KASFDEKALA ENYGAALEEV LRAKPTASKG RYLKKIVMTT TTGPGIPVDP SVTRNFTTVD A

NT Sequence

TTGGATGGAC ACATGAGCAA GAAGAGCAAG GCCTACCGCG AAGCCGCCGC GAAGGTGGAC CGGGACCGGC TGTACCACCC GCTGGAGGCC GCGAAGCTGG CCAAGGAGAC CTCGTCGAAG AAGCAGGACG CCACCGTCGA GGTGGCCATC CGGCTCGGCG TGGACCCGCG CAAGGCCGAT CAGATGGTGC GCGGCACCGT GAACCTGCCG CACGGCACCG GTAAGACCGC CCGGGTCGCG GTGTTCGCCG TCGGTGAGAA GGCCGAGGAG GCGAAGGCCG CGGGCGCGGA CATTGTCGGC AGCGACGACC TGATCGAGCA GATCCAGGGC GGCATGCTCG ACTTCGACGC GGCCATCGCC ACCCCCGACC AGATGGCGAA GGTCGGCCGG ATCGCCCGCA TCCTGGGCCC GCGTGGTCTG ATGCCCAACC CGAAGACCGG CACCGTCACC ACCGACATCG CCAAGGCCGT GCAGGACATC AAGGGCGGCA AGATCAACTT CCGCGTCGAC AAGGCCGCGA ACCTGCACCT GGTGATCGGC AAAGCGTCGT TCGACGAGAA GGCGCTGGCG GAGAACTACG GCGCGGCGCT GGAAGAGGTG CTGCGCGCCA AGCCGACCGC CTCCAAGGGC CGCTACCTCA AGAAGATCGT GATGACCACG ACGACGGGCC CCGGCATCCC GGTCGATCCG TCGGTGACCC GGAACTTCAC CACGGTCGAC GCC

Details for MythA.00328.a.A1.GE28726

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDGHMSKK SKAYREAAAK VDRDRLYHPL EAAKLAKETS SKKQDATVEV AIRLGVDPRK ADQMVRGTVN LPHGTGKTAR VAVFAVGEKA EEAKAAGADI VGSDDLIEQI QGGMLDFDAA IATPDQMAKV GRIARILGPR GLMPNPKTGT VTTDIAKAVQ DIKGGKINFR VDKAANLHLV IGKASFDEKA LAENYGAALE EVLRAKPTAS KGRYLKKIVM TTTTGPGIPV DPSVTRNFTT V

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg atggacacat gagcaagaag agcaaggcct accgcgaagc cgccgcgaag gtggaccggg accggctgta ccacccgctg gaggccgcga agctggccaa ggagacctcg tcgaagaagc aggacgccac cgtcgaggtg gccatccggc tcggcgtgga cccgcgcaag gccgatcaga tggtgcgcgg caccgtgaac ctgccgcacg gcaccggtaa gaccgcccgg gtcgcggtgt tcgccgtcgg tgagaaggcc gaggaggcga aggccgcggg cgcggacatt gtcggcagcg acgacctgat cgagcagatc cagggcggca tgctcgactt cgacgcggcc atcgccaccc ccgaccagat ggcgaaggtc ggccggatcg cccgcatcct gggcccgcgt ggtctgatgc ccaacccgaa gaccggcacc gtcaccaccg acatcgccaa ggccgtgcag gacatcaagg gcggcaagat caacttccgc gtcgacaagg ccgcgaacct gcacctggtg atcggcaaag cgtcgttcga cgagaaggcg ctggcggaga actacggcgc ggcgctggaa gaggtgctgc gcgccaagcc gaccgcctcc aagggccgct acctcaagaa gatcgtgatg accacgacga cgggccccgg catcccggtc gatccgtcgg tgacccggaa cttcaccacg gtcg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggatgga cacatgagca agaagagcaa ggcctaccgc gaagccgccg cgaaggtgga ccgggaccgg ctgtaccacc cgctggaggc cgcgaagctg gccaaggaga cctcgtcgaa gaagcaggac gccaccgtcg aggtggccat ccggctcggc gtggacccgc gcaaggccga tcagatggtg cgcggcaccg tgaacctgcc gcacggcacc ggtaagaccg cccgggtcgc ggtgttcgcc gtcggtgaga aggccgagga ggcgaaggcc gcgggcgcgg acattgtcgg cagcgacgac ctgatcgagc agatccaggg cggcatgctc gacttcgacg cggccatcgc cacccccgac cagatggcga aggtcggccg gatcgcccgc atcctgggcc cgcgtggtct gatgcccaac ccgaagaccg gcaccgtcac caccgacatc gccaaggccg tgcaggacat caagggcggc aagatcaact tccgcgtcga caaggccgcg aacctgcacc tggtgatcgg caaagcgtcg ttcgacgaga aggcgctggc ggagaactac ggcgcggcgc tggaagaggt gctgcgcgcc aagccgaccg cctccaaggg ccgctacctc aagaagatcg tgatgaccac gacgacgggc cccggcatcc cggtcgatcc gtcggtgacc cggaacttca ccacggtcga cgccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa