SSGCID: Target Page

MythA.00309.b

Putative acyl-CoA dehydrogenase (A0R1P4 homolog)

CENTER ID:	MythA.00309.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00309.b.A1.GE28725	Full length( MythA.00309.b )		1	457

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00309.b.A1.PS00579	Full length( MythA.00309.b )		1	457

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2416
RefSeq:	WP_003925942.1
UniProt:	G7CKG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSPTAPRTP SRREATMSWD FSTDSEYQEK LDWAEDFVRR EIFPLEALEL PQQQLVEAIT PLQQEVKRQG LWAAHLPPEM GGGGFGQVRL GLLHEIIGMS SLAPVVFGNN APDSGNAELL AIGIEEXXXX XXXXXXXXXX XXXXXXXXXX XXXTEPGAGA DPTLLTTTAE LIGDEWVING HKWFTSNGSA ADILLVMAVT DPDAAPRNRM SILVVPRDTP GVEIVRDIGT MQHPHPEFGR IGNHSEVVYR DVRVPRENLI GLRGNGFLLA QKRLGPGRIH HCMRWLGQSR RAFDMLCERS VSRYTHGSLL SEKQTVQNWI ADSMAEMGAA RLLTLHAAWK IDTQGVQAAL TDIAIIKFYG AQVLYNVIDR AIQVHGSLGV TTDLPLEAMY KEARNARIYD GPDEVHRQTV ARRVLRNYRS REVPSEHLPT RRAEAVKRFA RLLETGADVL TPSVQEK

NT Sequence

ATCGGGTCAC CGACTGCTCC ACGCACACCA TCTAGGAGAG AGGCAACCAT GAGCTGGGAC TTCTCCACCG ACTCCGAGTA TCAGGAGAAG CTGGACTGGG CCGAAGACTT CGTCCGGCGC GAGATCTTTC CGCTGGAAGC CCTGGAACTT CCGCAGCAGC AACTGGTGGA GGCGATCACC CCGCTCCAGC AGGAAGTCAA GCGGCAGGGC CTGTGGGCCG CGCACCTGCC GCCGGAGATG GGCGGCGGAG GTTTCGGGCA GGTTCGACTC GGTCTGTTGC ACGAGATCAT CGGGATGTCC TCACTCGCGC CGGTCGTGTT CGGCAACAAC GCACCGGATT CGGGGAATGC GGAGTTGCTG GCGATCGGGA TCGAGGAGNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGA CCGAACCCGG TGCCGGCGCA GACCCGACGC TGCTCACCAC CACCGCCGAG CTCATCGGAG ACGAGTGGGT GATCAACGGC CACAAGTGGT TCACATCCAA CGGTTCAGCC GCCGACATCC TGCTCGTGAT GGCGGTGACC GATCCGGATG CTGCGCCACG GAACCGGATG TCCATACTGG TGGTCCCCCG TGACACCCCG GGGGTGGAGA TCGTCCGGGA CATCGGGACG ATGCAGCATC CGCACCCCGA GTTCGGTCGG ATCGGCAATC ACAGTGAAGT GGTCTACCGG GATGTGCGGG TTCCCCGGGA GAACCTCATA GGGCTGCGTG GCAACGGTTT CCTGCTGGCA CAGAAGAGGT TGGGGCCGGG GCGGATCCAT CACTGCATGC GTTGGCTGGG CCAGTCCCGG CGGGCCTTCG ACATGCTGTG CGAACGCTCG GTGTCGCGCT ACACCCACGG CTCGCTGTTG TCGGAGAAGC AGACGGTGCA GAACTGGATT GCCGATTCGA TGGCCGAGAT GGGTGCCGCC CGGCTGCTGA CGCTGCACGC GGCCTGGAAG ATCGACACTC AAGGTGTGCA GGCTGCGCTG ACCGACATCG CGATCATCAA ATTCTACGGC GCGCAGGTTC TGTACAACGT CATCGACCGC GCCATCCAGG TGCACGGGTC GTTGGGGGTG ACGACCGACC TTCCGTTGGA GGCGATGTAC AAGGAGGCCC GCAACGCACG GATCTACGAC GGTCCCGATG AGGTGCACCG GCAGACCGTG GCCCGTCGCG TGCTCCGGAA CTACCGATCA CGCGAGGTTC CCAGTGAGCA CCTGCCGACC CGGCGCGCCG AAGCTGTGAA GCGCTTCGCC CGCCTGCTGG AGACCGGCGC CGACGTCCTC ACCCCGTCCG TGCAGGAGAA G

Details for MythA.00309.b.A1.GE28725

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	51

Validated AA Sequence

MSILVXPRDT PGVEIVRDIG TMQHPHPEFG RIGNHSEVVY RDVRVPRENL IGLRGNGFLL AQKRLGPGRI HHCMRWLGQS RRAFDMLCER SVSRYTHGSL LSEKQTVQNW IADSMAEMGA ARLLTLHAAW KIDTQGVQAA LTDIAIIKFY GAQVLYNVID RAIQVHGSLG VTTDLPLEAM YKEARNARIY DGPDEVHRQT VARRVLRNYR SREVPSEHLP TRRAEAVKRF ARLLETGADV LTPSVQ

Validated NT Sequence

cctgcacgga cggggtgagg acgtcggcgc cggtctccag caggcgggcg aagcgcttca cagcttcggc gcgccgggtc ggcaggtgct cactgggaac ctcgcgtgat cggtagttcc ggagcacgcg acgggccacg gtctgccggt gcacctcatc gggaccgtcg tagatccgtg cgttgcgggc ctccttgtac atcgcctcca acggaaggtc ggtcgtcacc cccaacgacc cgtgcacctg gatggcgcgg tcgatgacgt tgtacagaac ctgcgcgccg tagaatttga tgatcgcgat gtcggtcagc gcagcctgca caccttgagt gtcgatcttc caggccgcgt gcagcgtcag cagccgggcg gcacccatct cggccatcga atcggcaatc cagttctgca ccgtctgctt ctccgacaac agcgagccgt gggtgtagcg cgacaccgag cgttcgcaca gcatgtcgaa ggcccgccgg gactggccca gccaacgcat gcagtgatgg atccgccccg gccccaacct cttctgtgcc agcaggaaac cgttgccacg cagccctatg aggttctccc ggggaacccg cacatcccgg tagaccactt cactgtgatt gccgatccga ccgaactcgg ggtgcggatg ctgcatcgtc ccgatgtccc ggacgatctc cacccccggg gtgtcacggg ggancaccag tatggacatc cggttccgng gcgca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIGSPTAPR TPSRREATMS WDFSTDSEYQ EKLDWAEDFV RREIFPLEAL ELPQQQLVEA ITPLQQEVKR QGLWAAHLPP EMGGGGFGQV RLGLLHEIIG MSSLAPVVFG NNAPDSGNAE LLAIGIEEXX XXXXXXXXXX XXXXXXXXXX XXXXXTEPGA GADPTLLTTT AELIGDEWVI NGHKWFTSNG SAADILLVMA VTDPDAAPRN RMSILVVPRD TPGVEIVRDI GTMQHPHPEF GRIGNHSEVV YRDVRVPREN LIGLRGNGFL LAQKRLGPGR IHHCMRWLGQ SRRAFDMLCE RSVSRYTHGS LLSEKQTVQN WIADSMAEMG AARLLTLHAA WKIDTQGVQA ALTDIAIIKF YGAQVLYNVI DRAIQVHGSL GVTTDLPLEA MYKEARNARI YDGPDEVHRQ TVARRVLRNY RSREVPSEHL PTRRAEAVKR FARLLETGAD VLTPSVQEK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgggtca ccgactgctc cacgcacacc atctaggaga gaggcaacca tgagctggga cttctccacc gactccgagt atcaggagaa gctggactgg gccgaagact tcgtccggcg cgagatcttt ccgctggaag ccctggaact tccgcagcag caactggtgg aggcgatcac cccgctccag caggaagtca agcggcaggg cctgtgggcc gcgcacctgc cgccggagat gggcggcgga ggtttcgggc aggttcgact cggtctgttg cacgagatca tcgggatgtc ctcactcgcg ccggtcgtgt tcggcaacaa cgcaccggat tcggggaatg cggagttgct ggcgatcggg atcgaggagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg accgaacccg gtgccggcgc agacccgacg ctgctcacca ccaccgccga gctcatcgga gacgagtggg tgatcaacgg ccacaagtgg ttcacatcca acggttcagc cgccgacatc ctgctcgtga tggcggtgac cgatccggat gctgcgccac ggaaccggat gtccatactg gtggtccccc gtgacacccc gggggtggag atcgtccggg acatcgggac gatgcagcat ccgcaccccg agttcggtcg gatcggcaat cacagtgaag tggtctaccg ggatgtgcgg gttccccggg agaacctcat agggctgcgt ggcaacggtt tcctgctggc acagaagagg ttggggccgg ggcggatcca tcactgcatg cgttggctgg gccagtcccg gcgggccttc gacatgctgt gcgaacgctc ggtgtcgcgc tacacccacg gctcgctgtt gtcggagaag cagacggtgc agaactggat tgccgattcg atggccgaga tgggtgccgc ccggctgctg acgctgcacg cggcctggaa gatcgacact caaggtgtgc aggctgcgct gaccgacatc gcgatcatca aattctacgg cgcgcaggtt ctgtacaacg tcatcgaccg cgccatccag gtgcacgggt cgttgggggt gacgaccgac cttccgttgg aggcgatgta caaggaggcc cgcaacgcac ggatctacga cggtcccgat gaggtgcacc ggcagaccgt ggcccgtcgc gtgctccgga actaccgatc acgcgaggtt cccagtgagc acctgccgac ccggcgcgcc gaagctgtga agcgcttcgc ccgcctgctg gagaccggcg ccgacgtcct caccccgtcc gtgcaggaga agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MythA.00309.b.A1.PS00579

PURIFICATION DATe:	4/27/2010
CONCENTRATION:	37.36mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	51

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCGGGTCA CCGACTGCTC CACGCACACC ATCTAGGAGA GAGGCAACCA TGAGCTGGGA CTTCTCCACC GACTCCGAGT ATCAGGAGAA GCTGGACTGG GCCGAAGACT TCGTCCGGCG CGAGATCTTT CCGCTGGAAG CCCTGGAACT TCCGCAGCAG CAACTGGTGG AGGCGATCAC CCCGCTCCAG CAGGAAGTCA AGCGGCAGGG CCTGTGGGCC GCGCACCTGC CGCCGGAGAT GGGCGGCGGA GGTTTCGGGC AGGTTCGACT CGGTCTGTTG CACGAGATCA TCGGGATGTC CTCACTCGCG CCGGTCGTGT TCGGCAACAA CGCACCGGAT TCGGGGAATG CGGAGTTGCT GGCGATCGGG ATCGAGGAGN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG ACCGAACCCG GTGCCGGCGC AGACCCGACG CTGCTCACCA CCACCGCCGA GCTCATCGGA GACGAGTGGG TGATCAACGG CCACAAGTGG TTCACATCCA ACGGTTCAGC CGCCGACATC CTGCTCGTGA TGGCGGTGAC CGATCCGGAT GCTGCGCCAC GGAACCGGAT GTCCATACTG GTGGTCCCCC GTGACACCCC GGGGGTGGAG ATCGTCCGGG ACATCGGGAC GATGCAGCAT CCGCACCCCG AGTTCGGTCG GATCGGCAAT CACAGTGAAG TGGTCTACCG GGATGTGCGG GTTCCCCGGG AGAACCTCAT AGGGCTGCGT GGCAACGGTT TCCTGCTGGC ACAGAAGAGG TTGGGGCCGG GGCGGATCCA TCACTGCATG CGTTGGCTGG GCCAGTCCCG GCGGGCCTTC GACATGCTGT GCGAACGCTC GGTGTCGCGC TACACCCACG GCTCGCTGTT GTCGGAGAAG CAGACGGTGC AGAACTGGAT TGCCGATTCG ATGGCCGAGA TGGGTGCCGC CCGGCTGCTG ACGCTGCACG CGGCCTGGAA GATCGACACT CAAGGTGTGC AGGCTGCGCT GACCGACATC GCGATCATCA AATTCTACGG CGCGCAGGTT CTGTACAACG TCATCGACCG CGCCATCCAG GTGCACGGGT CGTTGGGGGT GACGACCGAC CTTCCGTTGG AGGCGATGTA CAAGGAGGCC CGCAACGCAC GGATCTACGA CGGTCCCGAT GAGGTGCACC GGCAGACCGT GGCCCGTCGC GTGCTCCGGA ACTACCGATC ACGCGAGGTT CCCAGTGAGC ACCTGCCGAC CCGGCGCGCC GAAGCTGTGA AGCGCTTCGC CCGCCTGCTG GAGACCGGCG CCGACGTCCT CACCCCGTCC GTGCAGGAGA AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA