SSGCID: Target Page

MythA.00309.a

Putative acyl-CoA dehydrogenase (A0R1P4 homolog)

CENTER ID:	MythA.00309.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00309.a.A1.GE28724	Full length( MythA.00309.a )		1	427

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00309.a.A1.PS00582	Full length( MythA.00309.a )		1	427

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1130
RefSeq:	WP_003924617.1
UniProt:	G7CDT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAWDFSTEPE FQEKLDWVKQ FCEEKVEPLD HVFPHAVRSP DPVVKAYVRE LQQEVRDQGL WAIFLDRELG GPGFGQLKLA LLNEIIGRYP GAPQMFGAAA PDTGNIEMLA AYGTEEQKKR WLEPLLNQDI FSAYSMTEPQ GGSDPNLFET HAVRDGDEWV INGEKWFTSA GRVADILFVM CTNGMFVVPR DTPGVEIMPE PRNHNHIIYR DVRVPLDHLL GPEDGAKVLA QRRLGGGRIH HAMRTIAQCN LAFDMMCERA LSRKSHGKVI AEHQMVQEKI AESYAMIRML RLYVLETAWK IDQTSTQEAR TDIAAVKFTM ARVLREVSFN ALHILGSLGT TDLTPLQAMY ANAPTMGIAD GVDEVHMATV ARRVLRDHAP HEGYFPTEFI PYKREAAWRK MKPVLDARPD LAASAEAYQR YFAIRRR

NT Sequence

ATGGCGTGGG ATTTCAGCAC CGAACCCGAG TTCCAGGAGA AGCTCGACTG GGTGAAGCAG TTCTGCGAGG AGAAGGTCGA ACCGCTCGAT CACGTGTTTC CCCACGCGGT GCGCTCACCC GACCCGGTGG TCAAGGCCTA TGTGCGTGAG CTGCAACAGG AGGTCAGGGA TCAGGGGCTG TGGGCGATCT TCCTCGACAG GGAACTCGGC GGCCCGGGAT TCGGTCAGCT CAAGCTCGCT CTGCTCAACG AGATCATCGG ACGCTATCCC GGCGCCCCGC AGATGTTCGG CGCGGCCGCA CCGGACACCG GCAACATCGA GATGCTCGCC GCCTACGGCA CCGAGGAGCA GAAGAAGCGC TGGCTCGAGC CGCTGCTGAA CCAGGACATC TTCTCGGCCT ACTCGATGAC CGAACCGCAG GGCGGCAGCG ATCCGAACCT GTTCGAAACC CACGCCGTGC GTGACGGTGA CGAATGGGTG ATCAACGGGG AGAAGTGGTT CACCTCGGCG GGCCGCGTCG CCGACATCCT GTTCGTGATG TGCACCAACG GCATGTTCGT GGTGCCGCGC GACACCCCGG GTGTGGAGAT CATGCCCGAA CCGCGCAACC ACAACCACAT CATCTACCGC GACGTGCGGG TGCCGCTCGA CCACCTGCTG GGGCCGGAGG ACGGCGCCAA GGTGCTGGCG CAGCGCCGGC TGGGCGGCGG GCGCATCCAC CACGCCATGC GCACCATCGC GCAGTGCAAT CTGGCGTTCG ACATGATGTG CGAACGGGCG TTGAGCCGGA AATCGCACGG CAAGGTCATC GCCGAACATC AGATGGTGCA GGAGAAGATC GCCGAGTCCT ACGCGATGAT CAGGATGCTG CGCCTGTACG TGCTGGAAAC CGCCTGGAAG ATCGACCAGA CCTCCACCCA GGAGGCGCGC ACCGACATCG CCGCGGTGAA ATTCACCATG GCGCGGGTGC TGCGCGAGGT GTCGTTCAAC GCGCTGCACA TCCTCGGCTC GCTGGGCACC ACCGACCTCA CCCCGCTGCA GGCGATGTAT GCCAACGCTC CTACGATGGG CATCGCCGAC GGCGTCGACG AGGTGCACAT GGCCACCGTC GCCCGCCGGG TGCTGCGGGA CCATGCGCCC CACGAAGGAT ATTTCCCTAC CGAATTCATC CCCTACAAGC GGGAAGCCGC GTGGCGGAAG ATGAAACCCG TGCTCGACGC GCGGCCGGAC CTCGCCGCAT CAGCGGAGGC GTACCAGAGG TACTTCGCCA TCCGGCGTCG C

Details for MythA.00309.a.A1.GE28724

HARVESTED ON:	3/1/2010
SEQUENCED ON:	3/5/2010
EXPECTED MW:	50kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAWDFSTEP EFQEKLDWVK QFCEEKVEPL DHVFPHAVRS PDPVVKAYVR ELQQEVRDQG LWAIFLDREL GGPGFGQLKL ALLNEIIGRY PGAPQMFGAA APDTGNIEML AAYGTEEQKK RWLEPLLNQD IFSAYSMTEP QGGSDPNLFE THAVRDGDEW VINGEKWFTS AGRVAXILFV MCTNGMFVVP RDTPGVEIMX XPXXXXXXXY XXVRVPLDHL LGPEDGAKVL AQRRLGGGRI HHAMRTIAQC NXAXDMMCER ALSRKSHGKV IAEHQMVQEK IAESYAMIRM LRLYVLETAW KIDQTSTQEA RTDIAAVKFT MARVLREVSF NALHILGSLG TTDLTPLQAM YANAPTMGIA DGVDEVHMAT VARRVLRDHA PHEGYFPTEF IPYKREAAWR KMKPVLDARP DLAASAEAYQ RYFAIRRR

Validated NT Sequence

accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg cgtgggattt cagcaccgaa cccgagttcc aggagaagct cgactgggtg aagcagttct gcgaggagaa ggtcgaaccg ctcgatcacg tgtttcccca cgcggtgcgc tcacccgacc cggtggtcaa ggcctatgtg cgtgagctgc aacaggaggt cagggatcag gggctgtggg cgatcttcct cgacagggaa ctcggcggcc cgggattcgg tcagctcaag ctcgctctgc tcaacgagat catcggacgc tatcccggcg ccccgcagat gttcggcgcg gccgcaccgg acaccggcaa catcgagatg ctcgccgcct acggcaccga ggagcagaag aagcgctggc tcgagccgct gctgaaccag gacatcttct cggcctactc gatgaccgaa ccgcagggcg gcagcgatcc gaacctgttc gaaacccacg ccgtgcgtga cggtgacgaa tgggtgatca acggggagaa gtggttcacc tcggcgggcc gcgtcgccna catcctgttc gtgatgtgca ccaacggcat gttcgtggtg ccgcgcgaca ccccgggtgt ggagatcatg ncnnaaccnn nnnacnncna ccncntcann tacngnnacg tgcgggtgcc gctcgaccac ctgctggggc cggaggacgg cgccaaggtg ctggcgcagc gccggctggg cggcgggcgc atccaccacg ccatgcgcac catcgcgcag tgcaatnngg cgttngacat gatgtgcgaa cgggcgttga gccggaaatc gcacggcaag gtcatcgccg aacatcagat ggtgcaggag aagatcgccg agtcctacgc gatgatcagg atgctgcgcc tgtacgtgct ggaaaccgcc tggaagatcg accagacctc cacccaggag gcgcgcaccg acatcgccgc ggtgaaattc accatggcgc gggtgctgcg cgaggtgtcg ttcaacgcgc tgcacatcct cggctcgctg ggcaccaccg acctcacccc gctgcaggcg atgtatgcca acgctcctac gatgggcatc gccgacggcg tcgacgaggt gcacatggcc accgtcgccc gccgggtgct gcgggaccat gcgccccacg aaggatattt ccctaccgaa ttcatcccct acaagcggga agccgcgtgg cggaagatga aacccgtgct cgacgcgcgg ccggacctcg ccgcatcagc ggaggcgtac cagaggtact tcgccatccg gcgtcgctaa taa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAWDFSTEP EFQEKLDWVK QFCEEKVEPL DHVFPHAVRS PDPVVKAYVR ELQQEVRDQG LWAIFLDREL GGPGFGQLKL ALLNEIIGRY PGAPQMFGAA APDTGNIEML AAYGTEEQKK RWLEPLLNQD IFSAYSMTEP QGGSDPNLFE THAVRDGDEW VINGEKWFTS AGRVADILFV MCTNGMFVVP RDTPGVEIMP EPRNHNHIIY RDVRVPLDHL LGPEDGAKVL AQRRLGGGRI HHAMRTIAQC NLAFDMMCER ALSRKSHGKV IAEHQMVQEK IAESYAMIRM LRLYVLETAW KIDQTSTQEA RTDIAAVKFT MARVLREVSF NALHILGSLG TTDLTPLQAM YANAPTMGIA DGVDEVHMAT VARRVLRDHA PHEGYFPTEF IPYKREAAWR KMKPVLDARP DLAASAEAYQ RYFAIRRR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtgggat ttcagcaccg aacccgagtt ccaggagaag ctcgactggg tgaagcagtt ctgcgaggag aaggtcgaac cgctcgatca cgtgtttccc cacgcggtgc gctcacccga cccggtggtc aaggcctatg tgcgtgagct gcaacaggag gtcagggatc aggggctgtg ggcgatcttc ctcgacaggg aactcggcgg cccgggattc ggtcagctca agctcgctct gctcaacgag atcatcggac gctatcccgg cgccccgcag atgttcggcg cggccgcacc ggacaccggc aacatcgaga tgctcgccgc ctacggcacc gaggagcaga agaagcgctg gctcgagccg ctgctgaacc aggacatctt ctcggcctac tcgatgaccg aaccgcaggg cggcagcgat ccgaacctgt tcgaaaccca cgccgtgcgt gacggtgacg aatgggtgat caacggggag aagtggttca cctcggcggg ccgcgtcgcc gacatcctgt tcgtgatgtg caccaacggc atgttcgtgg tgccgcgcga caccccgggt gtggagatca tgcccgaacc gcgcaaccac aaccacatca tctaccgcga cgtgcgggtg ccgctcgacc acctgctggg gccggaggac ggcgccaagg tgctggcgca gcgccggctg ggcggcgggc gcatccacca cgccatgcgc accatcgcgc agtgcaatct ggcgttcgac atgatgtgcg aacgggcgtt gagccggaaa tcgcacggca aggtcatcgc cgaacatcag atggtgcagg agaagatcgc cgagtcctac gcgatgatca ggatgctgcg cctgtacgtg ctggaaaccg cctggaagat cgaccagacc tccacccagg aggcgcgcac cgacatcgcc gcggtgaaat tcaccatggc gcgggtgctg cgcgaggtgt cgttcaacgc gctgcacatc ctcggctcgc tgggcaccac cgacctcacc ccgctgcagg cgatgtatgc caacgctcct acgatgggca tcgccgacgg cgtcgacgag gtgcacatgg ccaccgtcgc ccgccgggtg ctgcgggacc atgcgcccca cgaaggatat ttccctaccg aattcatccc ctacaagcgg gaagccgcgt ggcggaagat gaaacccgtg ctcgacgcgc ggccggacct cgccgcatca gcggaggcgt accagaggta cttcgccatc cggcgtcgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MythA.00309.a.A1.PS00582

PURIFICATION DATe:	5/2/2010
CONCENTRATION:	58.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAWDFSTEP EFQEKLDWVK QFCEEKVEPL DHVFPHAVRS PDPVVKAYVR ELQQEVRDQG LWAIFLDREL GGPGFGQLKL ALLNEIIGRY PGAPQMFGAA APDTGNIEML AAYGTEEQKK RWLEPLLNQD IFSAYSMTEP QGGSDPNLFE THAVRDGDEW VINGEKWFTS AGRVADILFV MCTNGMFVVP RDTPGVEIMP EPRNHNHIIY RDVRVPLDHL LGPEDGAKVL AQRRLGGGRI HHAMRTIAQC NLAFDMMCER ALSRKSHGKV IAEHQMVQEK IAESYAMIRM LRLYVLETAW KIDQTSTQEA RTDIAAVKFT MARVLREVSF NALHILGSLG TTDLTPLQAM YANAPTMGIA DGVDEVHMAT VARRVLRDHA PHEGYFPTEF IPYKREAAWR KMKPVLDARP DLAASAEAYQ RYFAIRRR

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTGGGAT TTCAGCACCG AACCCGAGTT CCAGGAGAAG CTCGACTGGG TGAAGCAGTT CTGCGAGGAG AAGGTCGAAC CGCTCGATCA CGTGTTTCCC CACGCGGTGC GCTCACCCGA CCCGGTGGTC AAGGCCTATG TGCGTGAGCT GCAACAGGAG GTCAGGGATC AGGGGCTGTG GGCGATCTTC CTCGACAGGG AACTCGGCGG CCCGGGATTC GGTCAGCTCA AGCTCGCTCT GCTCAACGAG ATCATCGGAC GCTATCCCGG CGCCCCGCAG ATGTTCGGCG CGGCCGCACC GGACACCGGC AACATCGAGA TGCTCGCCGC CTACGGCACC GAGGAGCAGA AGAAGCGCTG GCTCGAGCCG CTGCTGAACC AGGACATCTT CTCGGCCTAC TCGATGACCG AACCGCAGGG CGGCAGCGAT CCGAACCTGT TCGAAACCCA CGCCGTGCGT GACGGTGACG AATGGGTGAT CAACGGGGAG AAGTGGTTCA CCTCGGCGGG CCGCGTCGCC GACATCCTGT TCGTGATGTG CACCAACGGC ATGTTCGTGG TGCCGCGCGA CACCCCGGGT GTGGAGATCA TGCCCGAACC GCGCAACCAC AACCACATCA TCTACCGCGA CGTGCGGGTG CCGCTCGACC ACCTGCTGGG GCCGGAGGAC GGCGCCAAGG TGCTGGCGCA GCGCCGGCTG GGCGGCGGGC GCATCCACCA CGCCATGCGC ACCATCGCGC AGTGCAATCT GGCGTTCGAC ATGATGTGCG AACGGGCGTT GAGCCGGAAA TCGCACGGCA AGGTCATCGC CGAACATCAG ATGGTGCAGG AGAAGATCGC CGAGTCCTAC GCGATGATCA GGATGCTGCG CCTGTACGTG CTGGAAACCG CCTGGAAGAT CGACCAGACC TCCACCCAGG AGGCGCGCAC CGACATCGCC GCGGTGAAAT TCACCATGGC GCGGGTGCTG CGCGAGGTGT CGTTCAACGC GCTGCACATC CTCGGCTCGC TGGGCACCAC CGACCTCACC CCGCTGCAGG CGATGTATGC CAACGCTCCT ACGATGGGCA TCGCCGACGG CGTCGACGAG GTGCACATGG CCACCGTCGC CCGCCGGGTG CTGCGGGACC ATGCGCCCCA CGAAGGATAT TTCCCTACCG AATTCATCCC CTACAAGCGG GAAGCCGCGT GGCGGAAGAT GAAACCCGTG CTCGACGCGC GGCCGGACCT CGCCGCATCA GCGGAGGCGT ACCAGAGGTA CTTCGCCATC CGGCGTCGCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A