SSGCID: Target Page

MythA.00211.a

Pyrroline-5-carboxylate reductase (EC 1.5.1.2) (Rv0500 ortholog)

CENTER ID:	MythA.00211.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00211.a.B1.GE37637	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00211.a.B1.PW37501	full length		1	287

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.3019
RefSeq:	WP_003926551.1
UniProt:	G7CHH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARIAIIGGG NIGEALLSGL LRAGRQVKDL VVAEKDPNRA KYLAEKYGVL VTSVGDAAES ATFIVIAVKP GDVEKVIDDI AEAAARADSG TDEQVFVSVA AGVGTGYYEA KLPAGAPVVR VMPNAPMVVG GGVSALAPGR FATPEQLKEV AGIFDAVGGV LTVTEAQMDA VTAVSGSGPA YFFLMAEAMV DAGVAVGLSR AVATGLVAQT MAGSAAMLLD RLDDAAKSAA GAAMDTSPAE LRAMVTSPGG TTAAGLRELE RGGLRAAVAN AVEAAKMRSE QLGITSE

NT Sequence

gtggcgagaa tcgcgatcat cggtggcgga aacatcggcg aagcccttct gtccggactg ctgcgggcgg gccgtcaggt caaggacctg gtggtcgccg agaaggaccc gaaccgggcg aaatatctcg ccgagaagta cggggtgctg gtgacctcgg tcggcgacgc ggccgagagc gccaccttca tcgtgatcgc cgtcaaaccc ggcgatgtgg agaaggtcat cgacgacatc gcggaggccg cggcgcgcgc cgacagcggc accgacgagc aggtgttcgt gtcggtggcc gccggggtgg gcaccggcta ctacgaggcc aagctgccgg ccggcgcccc ggtggtccgg gtgatgccca acgccccgat ggtggtcggc ggcggggtga gcgcgctggc gcccggccgg ttcgcgacgc ccgaacagct caaggaggtc gcggggatct tcgacgccgt cggcggggtg ctgaccgtga ccgaggcgca gatggacgcc gtcaccgcgg tgtccggctc cggcccggcc tacttcttcc tgatggccga ggcgatggtg gacgccgggg tggccgtcgg gctgtcccgc gcggtggcca ccggtctggt cgcccagacc atggccggtt cggcggcgat gctgttggac cgtctggacg acgccgcgaa atccgcggcc ggcgctgcga tggacaccag cccggccgag ttgcgtgcga tggttacctc gccgggcggt accaccgcgg ctggtctgcg agaactcgaa cgcggcggtt tgcgggccgc ggtcgccaat gcggtggagg ccgcgaaaat gcgctctgaa cagctcggaa ttacatccga gtaa

Details for MythA.00211.a.B1.GE37637

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	30kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV ARIAIIGGGN IGEALLSGLL RAGRQVKDLV VAEKDPNRAK YLAEKYGVLV TSVGDAAESA TFIVIAVKPG DVEKVIDDIA EAAARADSGT DEQVFVSVAA GVGTGYYEAK LPAGAPVVRV MPNAPMVVGG GVSALAPGRF ATPEQLKEVA GIFDAVGGVL TVTEAQMDAV TAVSGSGPAY FFLMAEAMVD AGVAVGLSRA VATGLVAQTM AGSAAMLLDR LDDAAKSAAG AAMDTSPAEL RAMVTSPGGT TAAGLRELER GGLRAAVANA VEAAKMRSEQ LGITSE

Validated NT Sequence

atcttactca cttactcgga tgtaattccg agctgttcag agcgcatttt cgcggcctcc accgcattgg cgaccgcggc ccgcaaaccg ccgcgttcga gttctcgcag accagccgcg gtggtaccgc ccggcgaggt aaccatcgca cgcaactcgg ccgggctggt gtccatcgca gcgccggccg cggatttcgc ggcgtcgtcc agacggtcca acagcatcgc cgccgaaccg gccatggtct gggcgaccag accggtggcc accgcgcggg acagcccgac ggccaccccg gcgtccacca tcgcctcggc catcaggaag aagtaggccg ggccggagcc ggacaccgcg gtgacggcgt ccatctgcgc ctcggtcacg gtcagcaccc cgccgacggc gtcgaagatc cccgcgacct ccttgagctg ttcgggcgtc gcgaaccggc cgggcgccag cgcgctcacc ccgccgccga ccaccatcgg ggcgttgggc atcacccgga ccaccggggc gccggccggc agcttggcct cgtagtagcc ggtgcccacc ccggcggcca ccgacacgaa cacctgctcg tcggtgccgc tgtcggcgcg cgccgcggcc tccgcgatgt cgtcgatgac cttctccaca tcgccgggtt tgacggcgat cacgatgaag gtggcgctct cggccgcgtc gccgaccgag gtcaccagca ccccgtactt ctcggcgaga tatttcgccc ggttcgggtc cttctcggcg accaccaggt ccttgacctg acggcccgcc cgcagcagtc cggacagaag ggcttcgccg atgtttccgc caccgatgat cgcgattctc gccaccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMA RIAIIGGGNI GEALLSGLLR AGRQVKDLVV AEKDPNRAKY LAEKYGVLVT SVGDAAESAT FIVIAVKPGD VEKVIDDIAE AAARADSGTD EQVFVSVAAG VGTGYYEAKL PAGAPVVRVM PNAPMVVGGG VSALAPGRFA TPEQLKEVAG IFDAVGGVLT VTEAQMDAVT AVSGSGPAYF FLMAEAMVDA GVAVGLSRAV ATGLVAQTMA GSAAMLLDRL DDAAKSAAGA AMDTSPAELR AMVTSPGGTT AAGLRELERG GLRAAVANAV EAAKMRSEQL GITSE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcga gaatcgcgat catcggtggc ggaaacatcg gcgaagccct tctgtccgga ctgctgcggg cgggccgtca ggtcaaggac ctggtggtcg ccgagaagga cccgaaccgg gcgaaatatc tcgccgagaa gtacggggtg ctggtgacct cggtcggcga cgcggccgag agcgccacct tcatcgtgat cgccgtcaaa cccggcgatg tggagaaggt catcgacgac atcgcggagg ccgcggcgcg cgccgacagc ggcaccgacg agcaggtgtt cgtgtcggtg gccgccgggg tgggcaccgg ctactacgag gccaagctgc cggccggcgc cccggtggtc cgggtgatgc ccaacgcccc gatggtggtc ggcggcgggg tgagcgcgct ggcgcccggc cggttcgcga cgcccgaaca gctcaaggag gtcgcgggga tcttcgacgc cgtcggcggg gtgctgaccg tgaccgaggc gcagatggac gccgtcaccg cggtgtccgg ctccggcccg gcctacttct tcctgatggc cgaggcgatg gtggacgccg gggtggccgt cgggctgtcc cgcgcggtgg ccaccggtct ggtcgcccag accatggccg gttcggcggc gatgctgttg gaccgtctgg acgacgccgc gaaatccgcg gccggcgctg cgatggacac cagcccggcc gagttgcgtg cgatggttac ctcgccgggc ggtaccaccg cggctggtct gcgagaactc gaacgcggcg gtttgcgggc cgcggtcgcc aatgcggtgg aggccgcgaa aatgcgctct gaacagctcg gaattacatc cgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MythA.00211.a.B1.PW37501

PURIFICATION DATe:	6/2/2014
CONCENTRATION:	8.61mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGCGA GAATCGCGAT CATCGGTGGC GGAAACATCG GCGAAGCCCT TCTGTCCGGA CTGCTGCGGG CGGGCCGTCA GGTCAAGGAC CTGGTGGTCG CCGAGAAGGA CCCGAACCGG GCGAAATATC TCGCCGAGAA GTACGGGGTG CTGGTGACCT CGGTCGGCGA CGCGGCCGAG AGCGCCACCT TCATCGTGAT CGCCGTCAAA CCCGGCGATG TGGAGAAGGT CATCGACGAC ATCGCGGAGG CCGCGGCGCG CGCCGACAGC GGCACCGACG AGCAGGTGTT CGTGTCGGTG GCCGCCGGGG TGGGCACCGG CTACTACGAG GCCAAGCTGC CGGCCGGCGC CCCGGTGGTC CGGGTGATGC CCAACGCCCC GATGGTGGTC GGCGGCGGGG TGAGCGCGCT GGCGCCCGGC CGGTTCGCGA CGCCCGAACA GCTCAAGGAG GTCGCGGGGA TCTTCGACGC CGTCGGCGGG GTGCTGACCG TGACCGAGGC GCAGATGGAC GCCGTCACCG CGGTGTCCGG CTCCGGCCCG GCCTACTTCT TCCTGATGGC CGAGGCGATG GTGGACGCCG GGGTGGCCGT CGGGCTGTCC CGCGCGGTGG CCACCGGTCT GGTCGCCCAG ACCATGGCCG GTTCGGCGGC GATGCTGTTG GACCGTCTGG ACGACGCCGC GAAATCCGCG GCCGGCGCTG CGATGGACAC CAGCCCGGCC GAGTTGCGTG CGATGGTTAC CTCGCCGGGC GGTACCACCG CGGCTGGTCT GCGAGAACTC GAACGCGGCG GTTTGCGGGC CGCGGTCGCC AATGCGGTGG AGGCCGCGAA AATGCGCTCT GAACAGCTCG GAATTACATC CGAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT