SSGCID: Target Page

MysmA.19304.a

Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX [Includes: Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS); Phosphatidylglycerol lysyltransferase (EC 2.3.2.3) (Lysylphosphatidylglycerol synthetase) (LPG synthetase)]

CENTER ID:	MysmA.19304.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.19304.a.B2.GE40889	soluble domain		232	1106

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3734
UniProt:	A0QYV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVISRVEHL PARPASRVAW VPAAAGWTVG VIATLSLIAS VSPLVRSLIR VPREFVNDFI FNFPDTSFAW AFVLALLAAA LAARKRIAWW VLVLYLVGAI GWNVGDLAAG GDTVADDIGE LIGIAFHVAA IVFLVVARKE FWAKVRRGAL LKSAAVLVAG NLIGIVVAWG LLQAFPGTLD PEWRLPYAVN RVSGFATVPT EVFEGYSHTF LNAIFGLFGA LALMAAAIVL FQSQRASNAL TGEDESAIRG LLELYGKNDS LGYFATRRDK SVVFAPNGRA AITYRVEVGV CLASGDPVGD PKSWPQAIAA WLQLCQAYGW APGVMGASLA GAEAYRAAGL NALQLGDEAI LHPDRFRLSG PDMRAVRQAV TRARRAGTSV RIRRHRELSP EEMAAVIRRA DAWRDTETER GFSMALGRLG DPADDDCLLV EAVQGDEVVA MLSLVPWGSN GVSLDVMRRS PRSPNGTIEL MVSELCMQAE DIGISRISLN FAMFRSAFEQ GAQLGAGPVA RLWRALLVFF SKWWQLESLY RSNMKYQPEW VPRYACYEDA RLVPRVGVAS VIAEGFLVLP FSRRNKQHTG EHIAAPDTLV QSGRLHRDGS APDVTGLQGE LPDADDEPRL PEQVRVRMAK LKALQGNGVD AYPVGQPPSH TVAQALGATD GETLSVAGRV MRIRDYGGVL FAQLRDWSGE VQLLLDNAAL TEGETADFTA TIDLGDLVEV TGSMGFSRNG TRSLLVERWR LIGKCLRPLP DKWKGLTDQE ARVRARYVDL AVNTEARDLI RARSGALHAI RETLYSKGFL EVETPILQQI HGGANARPFL THINAYDLDL YLRIAPELYL KRLCVGGVER VFELGRAFRN EGVDFSHNPE FTLLEAYQAH ADYNVWIDGC RELIQNAAQA ANGAQVFLRP RPNDPAGTLE PVDISGQWAV KTVHDAVSEA IGEHITPETE LGELRRLCDS AGIPYLTHWD AGAVVLEMYE HLVEDRTTEP TFYKDFPTSV SPLTRPHRSI PGVAERWDLV AWGVELGTAY SELTDPVEQR RRLQEQSLLA AGGDPEAMEL DEDFLQAMEY AMPPTGGLGM GVDRVVMMIT GRSIRETLPF PLAKPR

NT Sequence

atgaccgtaa tcagtcgcgt cgagcacctc ccggcgcgtc cggcctcccg agtggcctgg gtgcccgcgg cagcgggctg gaccgtcggc gtcatcgcga ccctctcgtt gatcgccagc gtctcgcctc tcgtgcgctc gctcatccgg gtgccgcggg agttcgtcaa cgacttcatc ttcaacttcc cggacaccag cttcgcctgg gcgttcgtcc tggcgctgct ggccgccgcg ctggcggccc gcaagcgcat cgcgtggtgg gtcctggtgc tctacctggt gggtgccatc ggctggaacg tgggcgatct cgcggcgggc ggcgacaccg tcgccgacga catcggcgag ctcatcggca tcgccttcca cgtcgcggcg atcgtgttcc tggtggtggc ccgcaaggag ttctgggcca aggtccgccg cggcgctctg ctcaagtcgg cggcggtgct ggtggccggc aacctgatcg gcatcgtcgt ggcgtgggga ctgctgcagg cgttcccggg cacccttgac cccgagtggc ggttgccgta cgcggtcaac cgggtcagcg ggttcgcgac cgtgcccacc gaggtgttcg agggttactc gcacacgttc ctcaacgcga tcttcggcct gttcggcgca ctggctttga tggccgcggc gatcgtgctg ttccaatcgc agcgcgcgtc gaacgcgctc accggtgagg acgagtccgc catccggggg ctgctcgagc tctacggcaa gaacgactcc ctcggctact tcgcgacccg ccgcgacaag tcggtggtgt tcgcgccgaa cggacgcgcc gcaatcacct accgcgtcga ggtcggcgtg tgcctggcca gtggcgaccc cgtcggtgac cccaagtcgt ggccgcaggc catcgcggcg tggttgcagt tgtgccaggc ctacggctgg gcacccggcg tgatgggcgc gagcctggcg ggcgccgagg cctaccgggc cgcgggtctc aacgcgctgc aactgggcga cgaggcgatc ctgcaccccg accgcttccg gttgtcggga cccgacatgc gcgccgtgcg ccaggccgtg acccgcgcgc gccgggcagg cacctcggtg cggatccggc ggcaccgcga actgtcaccc gaggagatgg ccgcggtgat ccggcgtgcc gacgcgtggc gcgacaccga gaccgaacgc ggcttctcga tggcgctggg ccggctgggc gatcccgccg acgacgactg tctgctggtc gaggccgtgc agggcgatga ggtggtggcg atgctgtcgc tcgtgccgtg gggcagcaac ggtgtgtcgc tcgacgtcat gcgacgctca ccgcggtcgc ccaacggcac catcgagctg atggtcagcg agctgtgcat gcaggccgag gacatcggca tcagccggat ctcattgaac ttcgcgatgt tccgctcggc gttcgagcag ggcgcgcagc tgggtgccgg gccggtcgcg cggctgtggc gggccctgct ggtgttcttc tccaaatggt ggcagctgga gagcctgtac cgctccaaca tgaagtacca gcccgagtgg gtgccgcggt acgcgtgtta cgaggacgcg cggctggtgc cgcgggtcgg tgtggcgtcg gtgattgcgg aaggctttct ggtgcttccg ttctcgcgac gcaacaagca acacaccggc gagcacatcg ccgcacccga caccctggtg cagtccgggc gactgcaccg cgacggcagc gcacccgacg tcaccgggct gcagggcgag ctgcccgacg ccgacgacga gccccgcctg cccgagcagg tccgcgtgcg catggccaaa ctcaaggcgt tgcagggcaa cggcgtcgac gcctaccccg tggggcaacc gcccagccac accgtcgctc aggcccttgg cgccaccgat ggcgagacgc tgtcggtggc cggccgtgtg atgcggatcc gcgactacgg cggcgtgctg ttcgcgcaac tgcgggactg gtcgggtgag gtccaattgc tgttggacaa cgccgcactg accgagggcg agaccgcgga cttcaccgcg acgatcgacc tgggcgacct cgtcgaggtg accggttcga tgggcttcag ccgcaacgga acccgctcgc tgctggtcga gcgctggcga ctgatcggca agtgcctgcg cccgctgccc gacaagtgga agggcctcac cgaccaggag gcccgtgtgc gggcccgcta tgtcgacctg gccgtcaaca ccgaggcgcg cgatctgatc cgcgcacgca gcggcgcgtt gcacgccatc cgggaaaccc tgtactccaa gggtttcctc gaggtcgaga ccccgatcct gcagcaaatc cacggcggcg ccaacgccag gccgttcttg acccacatca acgcctacga cctggacctg tatctgcgga tcgcacccga gctgtacctc aagcggttgt gcgtgggcgg tgtcgagcgg gtcttcgaac tgggccgcgc gttccgcaac gagggcgtcg acttcagcca caaccccgag ttcacactcc tggaggccta tcaggcgcac gccgactaca acgtgtggat cgacggctgc cgcgagctga tccagaacgc ggcacaggcc gccaacggtg cgcaggtgtt cctgcggccc cgcccgaacg atccggccgg gaccctcgag cccgtcgaca tctccgggca gtgggccgtc aagaccgtgc acgacgcggt ctccgaggcg atcggcgagc acatcacgcc cgagaccgag ctgggcgagc ttcggcgcct gtgtgattcg gccggtattc cctacctgac gcactgggac gccggcgcgg tggttctgga gatgtacgag cacctggtcg aggaccgcac caccgaaccc acgttctaca aggacttccc gacgtcggtg tcgccgctga cccggccgca ccgcagcatc ccgggggtcg ccgaacgctg ggacctggtg gcgtggggcg tcgaactcgg cacggcctac agcgagctga ccgatccggt cgagcagcgc cgccgcctgc aggagcagtc cctgcttgcg gccggtggtg atcccgaggc gatggaactc gacgaggact tcctgcaggc catggagtac gccatgccgc cgaccggcgg cctgggcatg ggcgtggacc gcgtggtcat gatgatcacc gggcgcagca tccgtgagac gctgccgttc ccgctcgcca aaccgcgtta g

Details for MysmA.19304.a.B2.GE40889

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	98kDa
OBSERVED MW:	98kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHQS QRASNALTGE DESAIRGLLE LYGKNDSLGY FATRRDKSVV FAPNGRAAIT YRVEVGVCLA SGDPVGDPKS WPQAIAAWLQ LCQAYGWAPG VMGASLAGAE AYRAAGLNAL QLGDEAILHP DRFRLSGPDM RAVRQAVTRA RRAGTSVRIR RHRELSPEEM AAVIRRADAW RDTETERGFS MALGRLGDPA DDDCLLVEAV QGDEVVAMLS LVPWGSNGVS LDVMRRSPRS PNGTIELMVS ELCMQAEDIG ISRISLNFAM FRSAFEQGAQ LGAGPVARLW RALLVFFSKW WQLESLYRSN MKYQPEWVPR YACYEDARLV PRVGVASVIA EGFLVLPFSR RNKQHTGEHI AAPDTLVQSG RLHRDGSAPD VTGLQGELPD ADDEPRLPEQ VRVRMAKLKA LQGNGVDAYP VGQPPSHTVA QALGATDGET LSVAGRVMRI RDYGGVLFAQ LRDWSGEVQL LLDNAALTEG ETADFTATID LGDLVEVTGS MGFSRNGTRS LLVERWRLIG KCLRPLPDKW KGLTDQEARV RARYVDLAVN TEARDLIRAR SGALHAIRET LYSKGFLEVE TPILQQIHGG ANARPFLTHI NAYDLDLYLR IAPELYLKRL CVGGVERVFE LGRAFRNEGV DFSHNPEFTL LEAYQAHADY NVWIDGCREL IQNAAQAANG AQVFLRPRPN DPAGTLEPVD ISGQWAVKTV HDAVSEAIGE HITPETELGE LRRLCDSAGI PYLTHWDAGA VVLEMYEHLV EDRTTEPTFY KDFPTSVSPL TRPHRSIPGV AERWDLVAWG VELGTAYSEL TDPVEQRRRL QEQSLLAAGG DPEAMELDED FLQAMEYAMP PTGGLGMGVD RVVMMITGRS IRETLPFPLA KPR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaatcgc agcgcgcgtc gaacgcgctc accggtgagg acgagtccgc catccggggg ctgctcgagc tctacggcaa gaacgactcc ctcggctact tcgcgacccg ccgcgacaag tcggtggtgt tcgcgccgaa cggacgcgcc gcaatcacct accgcgtcga ggtcggcgtg tgcctggcca gtggcgaccc cgtcggtgac cccaagtcgt ggccgcaggc catcgcggcg tggttgcagt tgtgccaggc ctacggctgg gcacccggcg tgatgggcgc gagcctggcg ggcgccgagg cctaccgggc cgcgggtctc aacgcgctgc aactgggcga cgaggcgatc ctgcaccccg accgcttccg gttgtcggga cccgacatgc gcgccgtgcg ccaggccgtg acccgcgcgc gccgggcagg cacctcggtg cggatccggc ggcaccgcga actgtcaccc gaggagatgg ccgcggtgat ccggcgtgcc gacgcgtggc gcgacaccga gaccgaacgc ggcttctcga tggcgctggg ccggctgggc gatcccgccg acgacgactg tctgctggtc gaggccgtgc agggcgatga ggtggtggcg atgctgtcgc tcgtgccgtg gggcagcaac ggtgtgtcgc tcgacgtcat gcgacgctca ccgcggtcgc ccaacggcac catcgagctg atggtcagcg agctgtgcat gcaggccgag gacatcggca tcagccggat ctcattgaac ttcgcgatgt tccgctcggc gttcgagcag ggcgcgcagc tgggtgccgg gccggtcgcg cggctgtggc gggccctgct ggtgttcttc tccaaatggt ggcagctgga gagcctgtac cgctccaaca tgaagtacca gcccgagtgg gtgccgcggt acgcgtgtta cgaggacgcg cggctggtgc cgcgggtcgg tgtggcgtcg gtgattgcgg aaggctttct ggtgcttccg ttctcgcgac gcaacaagca acacaccggc gagcacatcg ccgcacccga caccctggtg cagtccgggc gactgcaccg cgacggcagc gcacccgacg tcaccgggct gcagggcgag ctgcccgacg ccgacgacga gccccgcctg cccgagcagg tccgcgtgcg catggccaaa ctcaaggcgt tgcagggcaa cggcgtcgac gcctaccccg tggggcaacc gcccagccac accgtcgctc aggcccttgg cgccaccgat ggcgagacgc tgtcggtggc cggccgtgtg atgcggatcc gcgactacgg cggcgtgctg ttcgcgcaac tgcgggactg gtcgggtgag gtccaattgc tgttggacaa cgccgcactg accgagggcg agaccgcgga cttcaccgcg acgatcgacc tgggcgacct cgtcgaggtg accggttcga tgggcttcag ccgcaacgga acccgctcgc tgctggtcga gcgctggcga ctgatcggca agtgcctgcg cccgctgccc gacaagtgga agggcctcac cgaccaggag gcccgtgtgc gggcccgcta tgtcgacctg gccgtcaaca ccgaggcgcg cgatctgatc cgcgcacgca gcggcgcgtt gcacgccatc cgggaaaccc tgtactccaa gggtttcctc gaggtcgaga ccccgatcct gcagcaaatc cacggcggcg ccaacgccag gccgttcttg acccacatca acgcctacga cctggacctg tatctgcgga tcgcacccga gctgtacctc aagcggttgt gcgtgggcgg tgtcgagcgg gtcttcgaac tgggccgcgc gttccgcaac gagggcgtcg acttcagcca caaccccgag ttcacactcc tggaggccta tcaggcgcac gccgactaca acgtgtggat cgacggctgc cgcgagctga tccagaacgc ggcacaggcc gccaacggtg cgcaggtgtt cctgcggccc cgcccgaacg atccggccgg gaccctcgag cccgtcgaca tctccgggca gtgggccgtc aagaccgtgc acgacgcggt ctccgaggcg atcggcgagc acatcacgcc cgagaccgag ctgggcgagc ttcggcgcct gtgtgattcg gccggtattc cctacctgac gcactgggac gccggcgcgg tggttctgga gatgtacgag cacctggtcg aggaccgcac caccgaaccc acgttctaca aggacttccc gacgtcggtg tcgccgctga cccggccgca ccgcagcatc ccgggggtcg ccgaacgctg ggacctggtg gcgtggggcg tcgaactcgg cacggcctac agcgagctga ccgatccggt cgagcagcgc cgccgcctgc aggagcagtc cctgcttgcg gccggtggtg atcccgaggc gatggaactc gacgaggact tcctgcaggc catggagtac gccatgccgc cgaccggcgg cctgggcatg ggcgtggacc gcgtggtcat gatgatcacc gggcgcagca tccgtgagac gctgccgttc ccgctcgcca aaccgcgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat