SSGCID: Target Page

MysmA.18665.a

ABC1 family protein Bbc-1

CENTER ID:	MysmA.18665.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.18665.a.B2.GE40133	MythA crystallizable fragment		54	424

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.18665.a.MB1.PS02493	full length		1	473

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.1337
RefSeq:	WP_011727618.1
RefSeq:	YP_006566083.1
RefSeq:	YP_885740.1
UniProt:	A0QS52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTTPAKTKH REVARLDRVP LPAEAARIGA TGWQITRTGA RVAANIFGRG SLQQKIVKQV PKTFSDLGPT YVKFGQIIAS SPGAFGEPLS REFRGLLDRV PPANPDEVQK LIREELGDDP HKLFKTFDDT PFASASIAQV HYATLHSGEE VVVKIQRPGI RRRVAADLQI LKRGARLVEF AKLGQRLSAQ DVVADFADNL AEELDFRLEA QSMDAWVAHM HASPLGANIR VPTVYWDLTS ERVLTMERIT GVRIDDVAAI RKKGFDGTEL VKALLFSVFE GGLRHGLFHG DLHAGNLYVD DDGKIVFFDF GIMGRIDPRT RWLLRELVHA LLVKKDHAAA GKIVVLMGAV GTVKPEGQAA KDLEAFATPL TMKSLGDMSY AEIGRQLSAL ADAYDVKLPR ELVLIGKQFL YVERYMKLLA PKWQMMSDPQ LTGYFANFMV EVSREHKEID DVDEIPDIPE TGTETQAPSG DKA

NT Sequence

atgagcacta cgccggcgaa aaccaagcat cgcgaggtgg ccaggctgga ccgcgtgccg ctcccggccg aggccgcgcg catcggcgcg accggatggc agatcacccg caccggggcc cgcgtcgcgg ccaacatctt cggccgcgga tctcttcagc agaagatcgt caagcaggtt cccaagacct tctccgacct gggacccacc tacgtcaagt tcgggcagat catcgcgtcg agccccggcg ccttcggcga accgctgagc cgcgagttcc gcggcctgct ggatcgggtg cctccggcga acccggacga ggtgcagaag ctgatccgcg aggaactcgg tgacgatccg cacaagctgt tcaagacgtt cgacgacacg ccgttcgcct cggcctccat cgcgcaggtc cactacgcca cgctgcacag cggcgaggag gtcgtcgtca agatccagcg cccgggcatc cggcgccgcg tggccgccga cctgcagatc ctcaagcgcg gggcacgtct ggtcgaattc gccaagctgg gtcagcggtt gagcgcgcag gacgtcgtcg ccgacttcgc cgacaacctc gccgaggaac tcgacttccg gctcgaggcc cagtcgatgg acgcctgggt ggcgcacatg cacgcctcgc cgctgggcgc caacatccgc gtccccacgg tgtactggga cctgaccagc gagcgcgtgc tgaccatgga acgcatcacc ggtgtgcgca tcgacgacgt ggccgcgatc cgcaagaagg gtttcgacgg caccgaactg gtcaaggccc tgctgttcag cgtgttcgag ggcggcctgc ggcacggcct gttccacggc gacctgcacg cgggcaacct ctacgtcgac gacgacggca agatcgtgtt cttcgacttc ggcatcatgg gccgcatcga tccgcgcacg cgctggctgc tgcgtgaact ggtgcacgcc ctgctggtca agaaggacca cgccgcggcg ggcaagatcg tggtgctgat gggcgcggtc ggcaccgtca aacccgaggg ccaggccgcc aaggacctcg aggcgttcgc cacgccgctg accatgaagt cgctcggcga catgtcctac gccgagatcg gcaggcagct ctcggccctg gccgacgcct acgacgtcaa gctgccccgc gagctcgtgc tgatcggcaa gcagttcctc tacgtcgagc gctacatgaa gttgctcgcg cccaagtggc agatgatgag cgatccgcag ctcaccggtt acttcgccaa cttcatggtc gaggtgagcc gggagcacaa ggagatcgac gacgtcgacg agatcccaga catccccgag accggaaccg agacgcaggc gcccagcggg gacaaggcct ga

Details for MysmA.18665.a.B2.GE40133

HARVESTED ON:	4/6/2016
SEQUENCED ON:	4/13/2016
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ KIVKQVPKTF SDLXXTYVKF GQIIASSPGA FGEPLSREFR GLLDRVPPAN PDEVQKLIRE ELGDDPHKLF KTFDDTPFAS ASIAQVHYAT LHSGEEVVVK IQRPGIRRRV AADLQILKRG ARLVEFAKLG QRLSAQDVVA DFADNLAEEL DFRLEAQSMD AWVAHMHASP LGANIRVPTV YWDLTSERVL TMERITGVRI DDVAAIRKKG FDGTELVKAL LFSVFEGGLR HGLFHGDLHA GNLYVDDDGK IVFFDFGIMG RIDPRTRWLL RELVHALLVK KDHAAAGKIV VLMGAVGTVK PEGQAAKDLE AFATPLTMKS LGDMSYAEIG RQLSALADAY DVKLPRELVL IGKQFLYVER YX

Validated NT Sequence

tnatgtagcg ctcgacgtag aggaactgct tgccgatcag cacgagctcg cggggcagct tgacgtcgta ggcgtcggcc agggccgaga gctgcctgcc gatctcggcg taggacatgt cgccgagcga cttcatggtc agcggcgtgg cgaacgcctc gaggtccttg gcggcctggc cctcgggttt gacggtgccg accgcgccca tcagcaccac gatcttgccc gccgcggcgt ggtccttctt gaccagcagg gcgtgcacca gttcacgcag cagccagcgc gtgcgcggat cgatgcggcc catgatgccg aagtcgaaga acacgatctt gccgtcgtcg tcgacgtaga ggttgcccgc gtgcaggtcg ccgtggaaca ggccgtgccg caggccgccc tcgaacacgc tgaacagcag ggccttgacc agttcggtgc cgtcgaaacc cttcttgcgg atcgcggcca cgtcgtcgat gcgcacaccg gtgatgcgtt ccatggtcag cacgcgctcg ctggtcaggt cccagtacac cgtggggacg cggatgttgg cgcccagcgg cgaggcgtgc atgtgcgcca cccaggcgtc catcgactgg gcctcgagcc ggaagtcgag ttcctcggcg aggttgtcgg cgaagtcggc gacgacgtcc tgcgcgctca accgctgacc cagcttggcg aattcgacca gacgtgcccc gcgcttgagg atctgcaggt cggcggccac gcggcgccgg atgcccgggc gctggatctt gacgacgacc tcctcgccgc tgtgcagcgt ggcgtagtgg acctgcgcga tggaggccga ggcgaacggc gtgtcgtcga acgtcttgaa cagcttgtgc ggatcgtcac cgagttcctc gcggatcagc ttctgcacct cgtccgggtt cgccggaggc acccgatcca gcaggccgcg gaactcgcgg ctcagcggtt cgccgaaggc gccggggctc gacgcgatga tctgcccgaa cttgacgtag gtggnnnnca ggtcggagaa ggtcttggga acctgcttga cgatcttctg catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHQK IVKQVPKTFS DLGPTYVKFG QIIASSPGAF GEPLSREFRG LLDRVPPANP DEVQKLIREE LGDDPHKLFK TFDDTPFASA SIAQVHYATL HSGEEVVVKI QRPGIRRRVA ADLQILKRGA RLVEFAKLGQ RLSAQDVVAD FADNLAEELD FRLEAQSMDA WVAHMHASPL GANIRVPTVY WDLTSERVLT MERITGVRID DVAAIRKKGF DGTELVKALL FSVFEGGLRH GLFHGDLHAG NLYVDDDGKI VFFDFGIMGR IDPRTRWLLR ELVHALLVKK DHAAAGKIVV LMGAVGTVKP EGQAAKDLEA FATPLTMKSL GDMSYAEIGR QLSALADAYD VKLPRELVLI GKQFLYVERY MKLLAPKWQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagaaga tcgtcaagca ggttcccaag accttctccg acctgggacc cacctacgtc aagttcgggc agatcatcgc gtcgagcccc ggcgccttcg gcgaaccgct gagccgcgag ttccgcggcc tgctggatcg ggtgcctccg gcgaacccgg acgaggtgca gaagctgatc cgcgaggaac tcggtgacga tccgcacaag ctgttcaaga cgttcgacga cacgccgttc gcctcggcct ccatcgcgca ggtccactac gccacgctgc acagcggcga ggaggtcgtc gtcaagatcc agcgcccggg catccggcgc cgcgtggccg ccgacctgca gatcctcaag cgcggggcac gtctggtcga attcgccaag ctgggtcagc ggttgagcgc gcaggacgtc gtcgccgact tcgccgacaa cctcgccgag gaactcgact tccggctcga ggcccagtcg atggacgcct gggtggcgca catgcacgcc tcgccgctgg gcgccaacat ccgcgtcccc acggtgtact gggacctgac cagcgagcgc gtgctgacca tggaacgcat caccggtgtg cgcatcgacg acgtggccgc gatccgcaag aagggtttcg acggcaccga actggtcaag gccctgctgt tcagcgtgtt cgagggcggc ctgcggcacg gcctgttcca cggcgacctg cacgcgggca acctctacgt cgacgacgac ggcaagatcg tgttcttcga cttcggcatc atgggccgca tcgatccgcg cacgcgctgg ctgctgcgtg aactggtgca cgccctgctg gtcaagaagg accacgccgc ggcgggcaag atcgtggtgc tgatgggcgc ggtcggcacc gtcaaacccg agggccaggc cgccaaggac ctcgaggcgt tcgccacgcc gctgaccatg aagtcgctcg gcgacatgtc ctacgccgag atcggcaggc agctctcggc cctggccgac gcctacgacg tcaagctgcc ccgcgagctc gtgctgatcg gcaagcagtt cctctacgtc gagcgctaca tgaagttgct cgcgcccaag tggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for MysmA.18665.a.MB1.PS02493

PURIFICATION DATe:	9/17/2015
CONCENTRATION:	44.13mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSTTPAKTKH REVARLDRVP LPAEAARIGA TGWQITRTGA RVAANIFGRG SLQQKIVKQV PKTFSDLGPT YVKFGQIIAS SPGAFGEPLS REFRGLLDRV PPANPDEVQK LIREELGDDP HKLFKTFDDT PFASASIAQV HYATLHSGEE VVVKIQRPGI RRRVAADLQI LKRGARLVEF AKLGQRLSAQ DVVADFADNL AEELDFRLEA QSMDAWVAHM HASPLGANIR VPTVYWDLTS ERVLTMERIT GVRIDDVAAI RKKGFDGTEL VKALLFSVFE GGLRHGLFHG DLHAGNLYVD DDGKIVFFDF GIMGRIDPRT RWLLRELVHA LLVKKDHAAA GKIVVLMGAV GTVKPEGQAA KDLEAFATPL TMKSLGDMSY AEIGRQLSAL ADAYDVKLPR ELVLIGKQFL YVERYMKLLA PKWQMMSDPQ LTGYFANFMV EVSREHKEID DVDEIPDIPE TGTETQAPSG DKA

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGAG CACTACGCCG GCGAAAACCA AGCATCGCGA GGTGGCCAGG CTGGACCGCG TGCCGCTCCC GGCCGAGGCC GCGCGCATCG GCGCGACCGG ATGGCAGATC ACCCGCACCG GGGCCCGCGT CGCGGCCAAC ATCTTCGGCC GCGGATCTCT TCAGCAGAAG ATCGTCAAGC AGGTTCCCAA GACCTTCTCC GACCTGGGAC CCACCTACGT CAAGTTCGGG CAGATCATCG CGTCGAGCCC CGGCGCCTTC GGCGAACCGC TGAGCCGCGA GTTCCGCGGC CTGCTGGATC GGGTGCCTCC GGCGAACCCG GACGAGGTGC AGAAGCTGAT CCGCGAGGAA CTCGGTGACG ATCCGCACAA GCTGTTCAAG ACGTTCGACG ACACGCCGTT CGCCTCGGCC TCCATCGCGC AGGTCCACTA CGCCACGCTG CACAGCGGCG AGGAGGTCGT CGTCAAGATC CAGCGCCCGG GCATCCGGCG CCGCGTGGCC GCCGACCTGC AGATCCTCAA GCGCGGGGCA CGTCTGGTCG AATTCGCCAA GCTGGGTCAG CGGTTGAGCG CGCAGGACGT CGTCGCCGAC TTCGCCGACA ACCTCGCCGA GGAACTCGAC TTCCGGCTCG AGGCCCAGTC GATGGACGCC TGGGTGGCGC ACATGCACGC CTCGCCGCTG GGCGCCAACA TCCGCGTCCC CACGGTGTAC TGGGACCTGA CCAGCGAGCG CGTGCTGACC ATGGAACGCA TCACCGGTGT GCGCATCGAC GACGTGGCCG CGATCCGCAA GAAGGGTTTC GACGGCACCG AACTGGTCAA GGCCCTGCTG TTCAGCGTGT TCGAGGGCGG CCTGCGGCAC GGCCTGTTCC ACGGCGACCT GCACGCGGGC AACCTCTACG TCGACGACGA CGGCAAGATC GTGTTCTTCG ACTTCGGCAT CATGGGCCGC ATCGATCCGC GCACGCGCTG GCTGCTGCGT GAACTGGTGC ACGCCCTGCT GGTCAAGAAG GACCACGCCG CGGCGGGCAA GATCGTGGTG CTGATGGGCG CGGTCGGCAC CGTCAAACCC GAGGGCCAGG CCGCCAAGGA CCTCGAGGCG TTCGCCACGC CGCTGACCAT GAAGTCGCTC GGCGACATGT CCTACGCCGA GATCGGCAGG CAGCTCTCGG CCCTGGCCGA CGCCTACGAC GTCAAGCTGC CCCGCGAGCT CGTGCTGATC GGCAAGCAGT TCCTCTACGT CGAGCGCTAC ATGAAGTTGC TCGCGCCCAA GTGGCAGATG ATGAGCGATC CGCAGCTCAC CGGTTACTTC GCCAACTTCA TGGTCGAGGT GAGCCGGGAG CACAAGGAGA TCGACGACGT CGACGAGATC CCAGACATCC CCGAGACCGG AACCGAGACG CAGGCGCCCA GCGGGGACAA GGCCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA