MysmA.18631.a

Cell division protein FtsQ (Rv2151c ortholog)

CENTER ID: MysmA.18631.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18631.a.B2.GE37634 soluble domain 140 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4145
RefSeq: YP_006568877.1
RefSeq: YP_888502.1
UniProt: A0R014

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGTGPHGDP AEDPAGPDDT AAETDGPAEP TEPVDASEAE MTDTTETTAQ TGTTAEADAS EEFEGPRRRA RRERAERRAA RDRAMAIEQA RREAKRRAVA GALDPTKSVP RNTIRGLKVL MWAALVSVLA VALGLLLYFT PIMSARNVEV SGLAEIPQEE VLTAAAVAPG TPLLQVDTDA VAERVATIRR VATARVQREY PSTLKISIVE RVPVVVKDYP DGPHLFDRDG VDFATGPAPL ALPYLDADNP GPNDPATRAA LDVMMALPPD VAAQVGRIAA PSVASITLTL IDGRVVVWGT DDRTQEKALK LAALLTQPGT TYDVSSPDLP TVK
NT Sequence
gtgaccggga cgggtcccca cggtgacccc gctgaagatc ctgcaggccc cgacgacacc gcagccgaga ccgacgggcc ggccgaaccg acagaacctg ttgacgcttc cgaagctgag atgaccgaca ccaccgagac gaccgcacag accgggacca ccgccgaagc cgacgcctcc gaggagttcg agggtccgcg ccgccgcgcg cgccgcgagc gcgccgaacg gcgcgcggcc cgcgaccggg ccatggcgat cgagcaggcc cgccgcgagg ccaagcgccg cgcggtcgcc ggcgccctcg atccgacgaa atccgttccg cgcaacacga tccgcggtct caaggtgctg atgtgggcgg cgctggtcag cgtgctggcg gtggcgcttg gcctgctgct ctacttcacc ccgatcatgt cggcccgcaa cgtcgaggtc agcggactgg ccgagatccc gcaggaggag gtgctcacgg ccgccgcggt cgccccaggc accccgttgc tgcaagtcga caccgatgcc gtcgccgaac gggtcgcgac catccgccgc gtcgcgaccg cgcgtgtgca gcgcgagtac ccgtcgacgc tcaagatctc gatcgtcgag cgcgtgcccg tggtggtcaa ggactatccg gacggcccgc acctgttcga ccgcgacggc gtcgacttcg ccacgggccc cgcgccgttg gcgctgccgt atctcgacgc cgacaacccg ggcccgaacg atccggcgac gcgcgcagcg ctcgacgtca tgatggcgct gccgccggac gttgccgcac aggtcggccg tatcgcggcg ccgtcggtcg cgtcgatcac gttgacgctc atcgacggtc gcgtcgtggt gtgggggacc gacgaccgta cccaggaaaa agcgctgaaa ctcgcggcgc tgttgaccca gccgggcacc acctacgacg tgtcgagccc ggacctgccg acggtcaaat ag
Details for MysmA.18631.a.B2.GE37634
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 21kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSARNVEVSG LAEIPQEEVL TAAAVAPGTP LLQVDTDAVA ERVATIRRVA TARVQREYPS TLKISIVERV PVVVKDYPDG PHLFDRDGVD FATGPAPLAL PYLDADNPGP NDPATRAALD VMMALPPDVA AQVGRIAAPS VASITLTLID GRVVVWGTDD RTQEKALKLA ALLTQPGTTY DVSSPDLPTV K
Validated NT Sequence
atcttactca cttatttgac cgtcggcagg tccgggctcg acacgtcgta ggtggtgccc ggctgggtca acagcgccgc gagtttcagc gctttttcct gggtacggtc gtcggtcccc cacaccacga cgcgaccgtc gatgagcgtc aacgtgatcg acgcgaccga cggcgccgcg atacggccga cctgtgcggc aacgtccggc ggcagcgcca tcatgacgtc gagcgctgcg cgcgtcgccg gatcgttcgg gcccgggttg tcggcgtcga gatacggcag cgccaacggc gcggggcccg tggcgaagtc gacgccgtcg cggtcgaaca ggtgcgggcc gtccggatag tccttgacca ccacgggcac gcgctcgacg atcgagatct tgagcgtcga cgggtactcg cgctgcacac gcgcggtcgc gacgcggcgg atggtcgcga cccgttcggc gacggcatcg gtgtcgactt gcagcaacgg ggtgcctggg gcgaccgcgg cggccgtgag cacctcctcc tgcgggatct cggccagtcc gctgacctcg acgttgcggg ccgacatgat cg
Expected Protein Sequence
MAHHHHHHTP IMSARNVEVS GLAEIPQEEV LTAAAVAPGT PLLQVDTDAV AERVATIRRV ATARVQREYP STLKISIVER VPVVVKDYPD GPHLFDRDGV DFATGPAPLA LPYLDADNPG PNDPATRAAL DVMMALPPDV AAQVGRIAAP SVASITLTLI DGRVVVWGTD DRTQEKALKL AALLTQPGTT YDVSSPDLPT VK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccccga tcatgtcggc ccgcaacgtc gaggtcagcg gactggccga gatcccgcag gaggaggtgc tcacggccgc cgcggtcgcc ccaggcaccc cgttgctgca agtcgacacc gatgccgtcg ccgaacgggt cgcgaccatc cgccgcgtcg cgaccgcgcg tgtgcagcgc gagtacccgt cgacgctcaa gatctcgatc gtcgagcgcg tgcccgtggt ggtcaaggac tatccggacg gcccgcacct gttcgaccgc gacggcgtcg acttcgccac gggccccgcg ccgttggcgc tgccgtatct cgacgccgac aacccgggcc cgaacgatcc ggcgacgcgc gcagcgctcg acgtcatgat ggcgctgccg ccggacgttg ccgcacaggt cggccgtatc gcggcgccgt cggtcgcgtc gatcacgttg acgctcatcg acggtcgcgt cgtggtgtgg gggaccgacg accgtaccca ggaaaaagcg ctgaaactcg cggcgctgtt gacccagccg ggcaccacct acgacgtgtc gagcccggac ctgccgacgg tcaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat