MysmA.18233.a

NADPH:adrenodoxin oxidoreductase fprA (NADPH-ferredoxin reductase)

CENTER ID: MysmA.18233.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18233.a.B1.GE40217 Full length(MysmA.18233.a) 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18233.a.B1.PS38153 Full length(MysmA.18233.a) 1 455

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.56.peg.2093
UniProt: I7FAD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPYHVAIVG SGPSGFFAAA SLLKFADSQP DRDVRVDMLE MLPTPWGLVR SGVAPDHPKI KSISAQFEKT AADPRFRFFG NVRVGEHVQP GELAERYDAV VYATGAQSDR ALNIPGEELP GSVAAVDFVG WYNAHPHFRE MAPDLSGGRA VVVGNGNVAL DVARILVSDP KALANTDIAD HALDKLDTRG VDEVVVLGRR GPLQATFTTL ELRELGDMEG LGDVDVILDP ADFADITDED LEAAGKTVKQ NIKVLRGYAE REPRGAKRRI VFRFFTSPIE LRGEDRVESI VLGRNELVRG ADGRMVAKDT GEREELPAQL VVRAVGYRGV PTPGLPFDER SGTIPHTDGR VEGSANEYVV GWIKRGPSGV IGSNKKDSQD TVNTLLDDLA AARLPQRGPD HSEKLAAWLL ERQPKVVTGE HWKLIDDYER AAGEPTGRPR VKLASVAELL RVGHG
NT Sequence
ATGCGCCCGT ACCACGTAGC GATCGTCGGC TCAGGACCCT CCGGATTCTT TGCTGCCGCA TCGCTGCTGA AGTTTGCCGA CTCTCAACCC GACCGCGATG TCCGCGTGGA CATGCTCGAG ATGCTGCCGA CCCCGTGGGG TCTGGTGCGC TCCGGCGTCG CTCCCGACCA TCCGAAGATC AAGTCGATCA GCGCCCAGTT CGAGAAGACC GCGGCCGACC CCCGCTTCCG GTTCTTCGGC AACGTCCGGG TCGGTGAGCA CGTCCAACCG GGCGAACTCG CCGAACGCTA CGACGCCGTC GTCTACGCCA CCGGCGCACA GTCCGACCGC GCGCTGAACA TCCCCGGCGA GGAGCTGCCG GGCAGCGTCG CGGCCGTCGA CTTCGTGGGT TGGTACAACG CGCATCCGCA TTTCCGCGAG ATGGCGCCCG ATCTCTCCGG CGGGCGCGCC GTGGTGGTCG GCAACGGCAA CGTCGCACTC GATGTCGCAC GCATCCTGGT CAGCGATCCG AAGGCGTTGG CCAACACCGA CATTGCCGAC CATGCGCTGG ACAAGCTGGA CACGCGCGGT GTGGACGAGG TCGTGGTGCT CGGCCGGCGT GGTCCGCTGC AGGCGACGTT CACCACGCTG GAGTTGCGTG AACTCGGCGA CATGGAGGGC CTCGGCGACG TCGACGTGAT CCTGGATCCG GCCGATTTCG CCGACATCAC CGACGAGGAT CTCGAAGCCG CGGGCAAGAC CGTCAAGCAG AACATCAAGG TGCTGCGCGG TTACGCCGAG CGGGAACCGC GAGGCGCCAA GCGGCGCATC GTGTTCCGGT TCTTCACCTC ACCGATCGAG CTGCGCGGCG AGGACCGCGT GGAATCGATC GTGTTGGGAC GCAACGAACT CGTGCGCGGC GCCGACGGCA GGATGGTGGC CAAGGACACC GGTGAGCGCG AAGAACTGCC GGCACAGCTG GTGGTGCGTG CGGTCGGATA CCGCGGCGTG CCGACTCCAG GCCTGCCGTT CGACGAGCGT TCGGGCACCA TCCCGCACAC CGACGGCCGC GTCGAGGGCA GCGCCAACGA GTACGTGGTG GGCTGGATCA AGCGCGGACC GTCCGGCGTC ATCGGCAGCA ACAAGAAGGA CTCACAGGAC ACCGTCAACA CCCTGCTCGA CGACCTCGCC GCGGCCCGGC TGCCCCAGCG CGGGCCCGAC CACTCCGAGA AGCTCGCGGC GTGGTTGCTG GAACGTCAGC CCAAGGTGGT CACGGGCGAG CACTGGAAGC TGATCGACGA CTACGAGCGC GCCGCGGGCG AACCGACCGG CCGACCCCGG GTGAAGCTGG CGAGCGTGGC TGAACTGCTG CGCGTGGGCC ACGGC
Details for MysmA.18233.a.B1.GE40217
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 50kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMR PYHVAIVGSG PSGFFAAASL LKFADSQPDR DVRVDMLEML PTPWGLVRSG VAPDHPKIKS ISAQFEKTAA DPRFRFFGNV RVGEHVQPGE LAERYDAVVY ATGAQSDRAL NIPGEELPGS VAAVDFVGWY NAHPHFREMA PDLSGGRAVV VGNGNVALDV ARILVSDPKA LANTDIADHA LDKLDTRGVD EVVVLGRRGP LQATFTTLEL RELGDMEGLG DVDVILDPAD FADITDEDLE AAGKTVKQNI KVLRGYAERE PRGAKRRIVF RFFTSPIELR GEDRVESIVL GRNELVRGAD GRMVAKDTGE REELPAQLVV RAVGYRGVPT PGLPFDERSG TIPHTDGRVE GSANEYVVGW IKRGPSGVIG SNKKDSQDTV NTLLDDLAAA RLPQRGPDHS EKLAAWLLER QPKVVTGEHW KLIDDYERAA GEPTGRPRVK LAS
Validated NT Sequence
cacgctcgcc agcttcaccc ggggtcggcc ggtcggttcg cccgcggcgc gctcgtagtc gtcgatcagc ttccagtgct cgcccgtgac caccttgggc tgacgttcca gcaaccacgc cgcgagcttc tcggagtggt cgggcccgcg ctggggcagc cgggccgcgg cgaggtcgtc gagcagggtg ttgacggtgt cctgtgagtc cttcttgttg ctgccgatga cgccggacgg tccgcgcttg atccagccca ccacgtactc gttggcgctg ccctcgacgc ggccgtcggt gtgcgggatg gtgcccgaac gctcgtcgaa cggcaggcct ggagtcggca cgccgcggta tccgaccgca cgcaccacca gctgtgccgg cagttcttcg cgctcaccgg tgtccttggc caccatcctg ccgtcggcgc cgcgcacgag ttcgttgcgt cccaacacga tcgattccac gcggtcctcg ccgcgcagct cgatcggtga ggtgaagaac cggaacacga tgcgccgctt ggcgcctcgc ggttcccgct cggcgtaacc gcgcagcacc ttgatgttct gcttgacggt cttgcccgcg gcttcgagat cctcgtcggt gatgtcggcg aaatcggccg gatccaggat cacgtcgacg tcgccgaggc cctccatgtc gccgagttca cgcaactcca gcgtggtgaa cgtcgcctgc agcggaccac gccggccgag caccacgacc tcgtccacac cgcgcgtgtc cagcttgtcc agcgcatggt cggcaatgtc ggtgttggcc aacgccttcg gatcgctgac caggatgcgt gcgacatcga gtgcgacgtt gccgttgccg accaccacgg cgcgcccgcc ggagagatcg ggcgccatct cgcggaaatg cggatgcgcg ttgtaccaac ccacgaagtc gacggccgcg acgctgcccg gcagctcctc gccggggatg ttcagcgcgc ggtcggactg tgcgccggtg gcgtagacga cggcgtcgta gcgttcggcg agttcgcccg gttggacgtg ctcaccgacc cggacgttgc cgaagaaccg gaagcggggg tcggccgcgg tcttctcgaa ctgggcgctg atcgacttga tcttcggatg gtcgggagcg acgccggagc gcaccagacc ccacggggtc ggcagcatct cgagcatgtc cacgcggaca tcgcggtcgg gttgagagtc ggcaaacttc agcagcgatg cggcagcaaa gaatccggag ggtcctgagc cgacgatcgc tacgtggtac gggcgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMR PYHVAIVGSG PSGFFAAASL LKFADSQPDR DVRVDMLEML PTPWGLVRSG VAPDHPKIKS ISAQFEKTAA DPRFRFFGNV RVGEHVQPGE LAERYDAVVY ATGAQSDRAL NIPGEELPGS VAAVDFVGWY NAHPHFREMA PDLSGGRAVV VGNGNVALDV ARILVSDPKA LANTDIADHA LDKLDTRGVD EVVVLGRRGP LQATFTTLEL RELGDMEGLG DVDVILDPAD FADITDEDLE AAGKTVKQNI KVLRGYAERE PRGAKRRIVF RFFTSPIELR GEDRVESIVL GRNELVRGAD GRMVAKDTGE REELPAQLVV RAVGYRGVPT PGLPFDERSG TIPHTDGRVE GSANEYVVGW IKRGPSGVIG SNKKDSQDTV NTLLDDLAAA RLPQRGPDHS EKLAAWLLER QPKVVTGEHW KLIDDYERAA GEPTGRPRVK LASVAELLRV GHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc cgtaccacgt agcgatcgtc ggctcaggac cctccggatt ctttgctgcc gcatcgctgc tgaagtttgc cgactctcaa cccgaccgcg atgtccgcgt ggacatgctc gagatgctgc cgaccccgtg gggtctggtg cgctccggcg tcgctcccga ccatccgaag atcaagtcga tcagcgccca gttcgagaag accgcggccg acccccgctt ccggttcttc ggcaacgtcc gggtcggtga gcacgtccaa ccgggcgaac tcgccgaacg ctacgacgcc gtcgtctacg ccaccggcgc acagtccgac cgcgcgctga acatccccgg cgaggagctg ccgggcagcg tcgcggccgt cgacttcgtg ggttggtaca acgcgcatcc gcatttccgc gagatggcgc ccgatctctc cggcgggcgc gccgtggtgg tcggcaacgg caacgtcgca ctcgatgtcg cacgcatcct ggtcagcgat ccgaaggcgt tggccaacac cgacattgcc gaccatgcgc tggacaagct ggacacgcgc ggtgtggacg aggtcgtggt gctcggccgg cgtggtccgc tgcaggcgac gttcaccacg ctggagttgc gtgaactcgg cgacatggag ggcctcggcg acgtcgacgt gatcctggat ccggccgatt tcgccgacat caccgacgag gatctcgaag ccgcgggcaa gaccgtcaag cagaacatca aggtgctgcg cggttacgcc gagcgggaac cgcgaggcgc caagcggcgc atcgtgttcc ggttcttcac ctcaccgatc gagctgcgcg gcgaggaccg cgtggaatcg atcgtgttgg gacgcaacga actcgtgcgc ggcgccgacg gcaggatggt ggccaaggac accggtgagc gcgaagaact gccggcacag ctggtggtgc gtgcggtcgg ataccgcggc gtgccgactc caggcctgcc gttcgacgag cgttcgggca ccatcccgca caccgacggc cgcgtcgagg gcagcgccaa cgagtacgtg gtgggctgga tcaagcgcgg accgtccggc gtcatcggca gcaacaagaa ggactcacag gacaccgtca acaccctgct cgacgacctc gccgcggccc ggctgcccca gcgcgggccc gaccactccg agaagctcgc ggcgtggttg ctggaacgtc agcccaaggt ggtcacgggc gagcactgga agctgatcga cgactacgag cgcgccgcgg gcgaaccgac cggccgaccc cgggtgaagc tggcgagcgt ggctgaactg ctgcgcgtgg gccacggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MysmA.18233.a.B1.PS38153
PURIFICATION DATe: 12/20/2016
CONCENTRATION: 41mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR PYHVAIVGSG PSGFFAAASL LKFADSQPDR DVRVDMLEML PTPWGLVRSG VAPDHPKIKS ISAQFEKTAA DPRFRFFGNV RVGEHVQPGE LAERYDAVVY ATGAQSDRAL NIPGEELPGS VAAVDFVGWY NAHPHFREMA PDLSGGRAVV VGNGNVALDV ARILVSDPKA LANTDIADHA LDKLDTRGVD EVVVLGRRGP LQATFTTLEL RELGDMEGLG DVDVILDPAD FADITDEDLE AAGKTVKQNI KVLRGYAERE PRGAKRRIVF RFFTSPIELR GEDRVESIVL GRNELVRGAD GRMVAKDTGE REELPAQLVV RAVGYRGVPT PGLPFDERSG TIPHTDGRVE GSANEYVVGW IKRGPSGVIG SNKKDSQDTV NTLLDDLAAA RLPQRGPDHS EKLAAWLLER QPKVVTGEHW KLIDDYERAA GEPTGRPRVK LAS
Validated NT Sequence
cacgctcgcc agcttcaccc ggggtcggcc ggtcggttcg cccgcggcgc gctcgtagtc gtcgatcagc ttccagtgct cgcccgtgac caccttgggc tgacgttcca gcaaccacgc cgcgagcttc tcggagtggt cgggcccgcg ctggggcagc cgggccgcgg cgaggtcgtc gagcagggtg ttgacggtgt cctgtgagtc cttcttgttg ctgccgatga cgccggacgg tccgcgcttg atccagccca ccacgtactc gttggcgctg ccctcgacgc ggccgtcggt gtgcgggatg gtgcccgaac gctcgtcgaa cggcaggcct ggagtcggca cgccgcggta tccgaccgca cgcaccacca gctgtgccgg cagttcttcg cgctcaccgg tgtccttggc caccatcctg ccgtcggcgc cgcgcacgag ttcgttgcgt cccaacacga tcgattccac gcggtcctcg ccgcgcagct cgatcggtga ggtgaagaac cggaacacga tgcgccgctt ggcgcctcgc ggttcccgct cggcgtaacc gcgcagcacc ttgatgttct gcttgacggt cttgcccgcg gcttcgagat cctcgtcggt gatgtcggcg aaatcggccg gatccaggat cacgtcgacg tcgccgaggc cctccatgtc gccgagttca cgcaactcca gcgtggtgaa cgtcgcctgc agcggaccac gccggccgag caccacgacc tcgtccacac cgcgcgtgtc cagcttgtcc agcgcatggt cggcaatgtc ggtgttggcc aacgccttcg gatcgctgac caggatgcgt gcgacatcga gtgcgacgtt gccgttgccg accaccacgg cgcgcccgcc ggagagatcg ggcgccatct cgcggaaatg cggatgcgcg ttgtaccaac ccacgaagtc gacggccgcg acgctgcccg gcagctcctc gccggggatg ttcagcgcgc ggtcggactg tgcgccggtg gcgtagacga cggcgtcgta gcgttcggcg agttcgcccg gttggacgtg ctcaccgacc cggacgttgc cgaagaaccg gaagcggggg tcggccgcgg tcttctcgaa ctgggcgctg atcgacttga tcttcggatg gtcgggagcg acgccggagc gcaccagacc ccacggggtc ggcagcatct cgagcatgtc cacgcggaca tcgcggtcgg gttgagagtc ggcaaacttc agcagcgatg cggcagcaaa gaatccggag ggtcctgagc cgacgatcgc tacgtggtac gggcgcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMR PYHVAIVGSG PSGFFAAASL LKFADSQPDR DVRVDMLEML PTPWGLVRSG VAPDHPKIKS ISAQFEKTAA DPRFRFFGNV RVGEHVQPGE LAERYDAVVY ATGAQSDRAL NIPGEELPGS VAAVDFVGWY NAHPHFREMA PDLSGGRAVV VGNGNVALDV ARILVSDPKA LANTDIADHA LDKLDTRGVD EVVVLGRRGP LQATFTTLEL RELGDMEGLG DVDVILDPAD FADITDEDLE AAGKTVKQNI KVLRGYAERE PRGAKRRIVF RFFTSPIELR GEDRVESIVL GRNELVRGAD GRMVAKDTGE REELPAQLVV RAVGYRGVPT PGLPFDERSG TIPHTDGRVE GSANEYVVGW IKRGPSGVIG SNKKDSQDTV NTLLDDLAAA RLPQRGPDHS EKLAAWLLER QPKVVTGEHW KLIDDYERAA GEPTGRPRVK LASVAELLRV GHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCC CGTACCACGT AGCGATCGTC GGCTCAGGAC CCTCCGGATT CTTTGCTGCC GCATCGCTGC TGAAGTTTGC CGACTCTCAA CCCGACCGCG ATGTCCGCGT GGACATGCTC GAGATGCTGC CGACCCCGTG GGGTCTGGTG CGCTCCGGCG TCGCTCCCGA CCATCCGAAG ATCAAGTCGA TCAGCGCCCA GTTCGAGAAG ACCGCGGCCG ACCCCCGCTT CCGGTTCTTC GGCAACGTCC GGGTCGGTGA GCACGTCCAA CCGGGCGAAC TCGCCGAACG CTACGACGCC GTCGTCTACG CCACCGGCGC ACAGTCCGAC CGCGCGCTGA ACATCCCCGG CGAGGAGCTG CCGGGCAGCG TCGCGGCCGT CGACTTCGTG GGTTGGTACA ACGCGCATCC GCATTTCCGC GAGATGGCGC CCGATCTCTC CGGCGGGCGC GCCGTGGTGG TCGGCAACGG CAACGTCGCA CTCGATGTCG CACGCATCCT GGTCAGCGAT CCGAAGGCGT TGGCCAACAC CGACATTGCC GACCATGCGC TGGACAAGCT GGACACGCGC GGTGTGGACG AGGTCGTGGT GCTCGGCCGG CGTGGTCCGC TGCAGGCGAC GTTCACCACG CTGGAGTTGC GTGAACTCGG CGACATGGAG GGCCTCGGCG ACGTCGACGT GATCCTGGAT CCGGCCGATT TCGCCGACAT CACCGACGAG GATCTCGAAG CCGCGGGCAA GACCGTCAAG CAGAACATCA AGGTGCTGCG CGGTTACGCC GAGCGGGAAC CGCGAGGCGC CAAGCGGCGC ATCGTGTTCC GGTTCTTCAC CTCACCGATC GAGCTGCGCG GCGAGGACCG CGTGGAATCG ATCGTGTTGG GACGCAACGA ACTCGTGCGC GGCGCCGACG GCAGGATGGT GGCCAAGGAC ACCGGTGAGC GCGAAGAACT GCCGGCACAG CTGGTGGTGC GTGCGGTCGG ATACCGCGGC GTGCCGACTC CAGGCCTGCC GTTCGACGAG CGTTCGGGCA CCATCCCGCA CACCGACGGC CGCGTCGAGG GCAGCGCCAA CGAGTACGTG GTGGGCTGGA TCAAGCGCGG ACCGTCCGGC GTCATCGGCA GCAACAAGAA GGACTCACAG GACACCGTCA ACACCCTGCT CGACGACCTC GCCGCGGCCC GGCTGCCCCA GCGCGGGCCC GACCACTCCG AGAAGCTCGC GGCGTGGTTG CTGGAACGTC AGCCCAAGGT GGTCACGGGC GAGCACTGGA AGCTGATCGA CGACTACGAG CGCGCCGCGG GCGAACCGAC CGGCCGACCC CGGGTGAAGC TGGCGAGCGT GGCTGAACTG CTGCGCGTGG GCCACGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT