MysmA.18013.a

Elongation factor G

CENTER ID: MysmA.18013.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18013.a.B1.GE40215 Full length(MysmA.18013.a) 1 701
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.56.peg.1408
UniProt: A4ZHU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQKDVLTDL NKVRNIGIMA HIDAGKTTTT ERILYYTGVN YKIGETHDGA STTDWMEQEQ ERGITITSAA VTCFWNNNQI NIIDTPGHVD FTVEVERSLR VLDGAVAVFD GKEGVEPQSE QVWRQADKYD VPRICFVNKM DKLGADFYFT VRTIEERLGA KPLVIQLPIG AENDFIGIID LVEMKAKVWR GETALGEKYE VEDIPADLAD KAEEYRTKLL ETVAESDEAL LEKYFGGEEL SVDEIKGAIR KLTVNSELYP VLCGSAFKNK GVQPMLDAVI DYLPSPLDVE SVQGHVPGKE DEVISRKPSV DEPFSALAFK IAVHPFFGKL TYVRVYSGVV ESGSQVVNST KGKKERLGKL FQMHANKENP VERASAGHIY AVIGLKDTTT GDTLCDPNEQ IVLESMTFPD PVIEVAIEPK TKSDQEKLGT AIQKLAEEDP TFKVHLDQET GQTVIGGMGE LHLDILVDRM RREFKVEANV GKPQVAYRET IKRKVEKVEY THKKQTGGSG QFAKVLIDLE PFVGEDGATY EFENKVTGGR IPREYIPSVD AGAQDAMQYG VLAGYPLVNL KVTLLDGAYH EVDSSEMAFK VAGSQALKKA AQAAQPVILE PIMAVEVTTP EDYMGEVIGD LNSRRGQIQA MEERSGARVV KAQVPLSEMF GYVGDLRSKT QGRANYSMVF DSYAEVPANV SKEIIAKATG Q
NT Sequence
GTGGCACAGA AGGACGTGCT TACCGACCTG AACAAGGTCC GCAACATCGG CATCATGGCG CACATCGATG CCGGCAAGAC CACGACGACC GAGCGCATCC TGTACTACAC CGGCGTCAAC TACAAGATCG GTGAGACGCA CGACGGTGCC TCCACGACCG ACTGGATGGA GCAGGAGCAG GAGCGTGGTA TCACCATCAC CTCCGCGGCT GTGACTTGCT TCTGGAACAA CAACCAGATC AACATCATCG ACACCCCCGG CCACGTCGAC TTCACCGTCG AGGTGGAGCG CTCGCTGCGC GTGCTCGACG GTGCGGTCGC CGTGTTCGAC GGCAAAGAAG GCGTGGAGCC GCAGTCCGAG CAGGTGTGGC GGCAGGCCGA CAAGTACGAC GTGCCGCGTA TCTGCTTCGT CAACAAGATG GACAAGCTCG GCGCGGACTT CTACTTCACC GTGCGCACCA TCGAGGAGCG CCTCGGTGCC AAGCCGCTGG TGATCCAGCT GCCGATCGGT GCCGAGAACG ACTTCATCGG CATCATCGAC CTGGTCGAGA TGAAGGCCAA GGTGTGGCGC GGCGAGACCG CCCTCGGTGA GAAGTACGAG GTCGAGGACA TCCCCGCGGA CCTCGCCGAC AAGGCCGAGG AGTACCGCAC CAAGCTGCTC GAGACCGTCG CCGAGAGCGA CGAGGCACTG CTGGAGAAGT ACTTCGGTGG CGAGGAGCTC TCGGTCGACG AGATCAAGGG CGCGATCCGC AAGCTGACGG TCAACAGCGA GCTGTACCCG GTGCTGTGCG GCAGCGCGTT CAAGAACAAG GGCGTGCAGC CCATGCTCGA CGCGGTCATC GACTACCTGC CGTCGCCGCT GGACGTCGAG TCCGTGCAGG GTCACGTGCC CGGCAAGGAA GACGAGGTCA TCAGCCGCAA GCCGTCGGTC GACGAGCCCT TCTCGGCGCT GGCGTTCAAG ATCGCGGTGC ACCCGTTCTT CGGCAAGCTG ACCTACGTCC GCGTGTACTC CGGTGTCGTG GAATCCGGTT CCCAGGTGGT CAACTCGACC AAGGGCAAGA AGGAGCGGCT GGGCAAGCTG TTCCAGATGC ACGCCAACAA GGAGAACCCG GTCGAACGGG CCTCCGCCGG CCACATCTAC GCCGTGATCG GTCTGAAGGA CACCACCACC GGCGACACGC TGTGCGATCC GAACGAGCAG ATCGTGCTCG AGTCGATGAC CTTCCCGGAT CCGGTCATCG AGGTGGCCAT CGAGCCCAAG ACCAAGAGTG ACCAGGAGAA GCTGGGCACC GCGATCCAGA AGCTCGCCGA AGAGGACCCG ACCTTCAAGG TGCACCTGGA CCAGGAGACC GGCCAGACCG TCATCGGCGG CATGGGCGAG CTGCACCTGG ACATCCTCGT GGACCGCATG CGTCGCGAGT TCAAGGTCGA GGCCAACGTC GGCAAGCCGC AGGTGGCCTA CCGCGAGACG ATCAAGCGCA AGGTCGAGAA GGTCGAGTAC ACCCACAAGA AGCAGACGGG TGGCTCGGGC CAGTTCGCGA AGGTGCTCAT CGATCTGGAG CCGTTCGTCG GCGAGGACGG TGCCACCTAC GAGTTCGAGA ACAAGGTCAC CGGTGGCCGC ATCCCGCGCG AGTACATCCC CTCGGTGGAC GCGGGCGCCC AGGACGCCAT GCAGTACGGC GTGCTCGCCG GCTACCCGCT GGTGAACCTG AAGGTGACGC TGCTCGACGG CGCCTACCAC GAGGTCGACT CCTCGGAAAT GGCGTTCAAA GTTGCGGGTT CACAGGCGTT GAAGAAGGCC GCTCAGGCGG CTCAGCCGGT GATCCTGGAG CCGATCATGG CGGTCGAGGT GACCACACCC GAGGACTACA TGGGTGAAGT GATCGGTGAC CTGAACTCCC GCCGTGGTCA GATCCAGGCC ATGGAGGAGC GCAGCGGTGC GCGCGTCGTC AAGGCGCAGG TGCCGCTGTC GGAGATGTTC GGCTACGTCG GCGACCTCCG GTCGAAGACC CAGGGCCGGG CGAACTACTC CATGGTGTTC GACTCGTACG CCGAAGTCCC GGCGAACGTG TCGAAGGAGA TCATCGCGAA GGCGACTGGC CAG
Details for MysmA.18013.a.B1.GE40215
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 78kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA QKDVLTDLNK VRNIGIMAHI DAGKTTTTER ILYYTGVNYK IGETHDGAST TDWMEQEQER GITITSAAVT CFWNNNQINI IDTPGHVDFT VEVERSLRVL DGAVAVFDGK EGVEPQSEQV WRQADKYDVP RICFVNKMDK LGADFYFTVR TIEERLGAKP LVIQLPIGAE NDFIGIIDLV EMKAKVWRGE TALGEKYEVE DIPADLADKA EEYRTKLLET VAESDEALLE KYFGGEELSV DEIKGAIRKL TVNSELYPVL CGSAFKNKGV QPMLDAVIDY LPSPLDVESV QGHVPGKEDE VISRKPSVDE PFSALAFKIA VHPFFGKLTY VRVYSGVVES GSQVVNSTKG KKERLGKLFQ MHANKENPVE RASAGHIYAV IGLKDTTTGD TLCDPNEQIV LESMTFPDPV IEVAIEPKTK SDQEKLGTAI QKLAEEDPTF KVHLDQETGQ TVIGGMGELH LDILVDRMRR EFKVEANVGK PQVAYRETIK RKVEKVEYTH KKQTGGSGQF AKVLIDLEPF VGEDGATYEF ENKVTGGRIP REYIPSVDAG AQDAMQYGVL AGYPLVNLKV TLLDGAYHEV DSSEMAFKVA GSQALKKAAQ AAQPVILEPI MAVEVTTPED YMGEVIGDLN SRRGQIQAME ERSGARVVKA QVPLSEMFGY VGDLRSKTQG RANYSMVFDS YAEVPANVSK EIIAKATGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcac agaaggacgt gcttaccgac ctgaacaagg tccgcaacat cggcatcatg gcgcacatcg atgccggcaa gaccacgacg accgagcgca tcctgtacta caccggcgtc aactacaaga tcggtgagac gcacgacggt gcctccacga ccgactggat ggagcaggag caggagcgtg gtatcaccat cacctccgcg gctgtgactt gcttctggaa caacaaccag atcaacatca tcgacacccc cggccacgtc gacttcaccg tcgaggtgga gcgctcgctg cgcgtgctcg acggtgcggt cgccgtgttc gacggcaaag aaggcgtgga gccgcagtcc gagcaggtgt ggcggcaggc cgacaagtac gacgtgccgc gtatctgctt cgtcaacaag atggacaagc tcggcgcgga cttctacttc accgtgcgca ccatcgagga gcgcctcggt gccaagccgc tggtgatcca gctgccgatc ggtgccgaga acgacttcat cggcatcatc gacctggtcg agatgaaggc caaggtgtgg cgcggcgaga ccgccctcgg tgagaagtac gaggtcgagg acatccccgc ggacctcgcc gacaaggccg aggagtaccg caccaagctg ctcgagaccg tcgccgagag cgacgaggca ctgctggaga agtacttcgg tggcgaggag ctctcggtcg acgagatcaa gggcgcgatc cgcaagctga cggtcaacag cgagctgtac ccggtgctgt gcggcagcgc gttcaagaac aagggcgtgc agcccatgct cgacgcggtc atcgactacc tgccgtcgcc gctggacgtc gagtccgtgc agggtcacgt gcccggcaag gaagacgagg tcatcagccg caagccgtcg gtcgacgagc ccttctcggc gctggcgttc aagatcgcgg tgcacccgtt cttcggcaag ctgacctacg tccgcgtgta ctccggtgtc gtggaatccg gttcccaggt ggtcaactcg accaagggca agaaggagcg gctgggcaag ctgttccaga tgcacgccaa caaggagaac ccggtcgaac gggcctccgc cggccacatc tacgccgtga tcggtctgaa ggacaccacc accggcgaca cgctgtgcga tccgaacgag cagatcgtgc tcgagtcgat gaccttcccg gatccggtca tcgaggtggc catcgagccc aagaccaaga gtgaccagga gaagctgggc accgcgatcc agaagctcgc cgaagaggac ccgaccttca aggtgcacct ggaccaggag accggccaga ccgtcatcgg cggcatgggc gagctgcacc tggacatcct cgtggaccgc atgcgtcgcg agttcaaggt cgaggccaac gtcggcaagc cgcaggtggc ctaccgcgag acgatcaagc gcaaggtcga gaaggtcgag tacacccaca agaagcagac gggtggctcg ggccagttcg cgaaggtgct catcgatctg gagccgttcg tcggcgagga cggtgccacc tacgagttcg agaacaaggt caccggtggc cgcatcccgc gcgagtacat cccctcggtg gacgcgggcg cccaggacgc catgcagtac ggcgtgctcg ccggctaccc gctggtgaac ctgaaggtga cgctgctcga cggcgcctac cacgaggtcg actcctcgga aatggcgttc aaagttgcgg gttcacaggc gttgaagaag gccgctcagg cggctcagcc ggtgatcctg gagccgatca tggcggtcga ggtgaccaca cccgaggact acatgggtga agtgatcggt gacctgaact cccgccgtgg tcagatccag gccatggagg agcgcagcgg tgcgcgcgtc gtcaaggcgc aggtgccgct gtcggagatg ttcggctacg tcggcgacct ccggtcgaag acccagggcc gggcgaacta ctccatggtg ttcgactcgt acgccgaagt cccggcgaac gtgtcgaagg agatcatcgc gaaggcgact ggccagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t