SSGCID: Target Page

MysmA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MysmA.17943.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17943.a.B1.GE42457	full length		1	953

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17943.a.B1.PW38502	full length		1	953

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.6734
UniProt:	A0R7H5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEPATTPTT PDEQIPRHRY NADLAGQIER AWQETWSDRG TFNVANPVGS LAPTDGSDVP ADKMFVQDMF PYPSGDGLHV GHPLGYIATD VYARYYRMLG RNVLHALGFD AFGLPAEQYA VQTGTHPRTR TEANIVNFRR QLGRLGLGHD TRRSFSTTDV DYYKWTQWIF LQIYNAWFDR DQNKARRISE LVEEFESGKR TLDDGRNWAD LSKGERADVI DGYRLVYRAD SMVNWCPGLG TVLANEEVTS EGRSDRGNFP VFRKRLRQWM MRITAYSDRL LEDLDVLDWP EKVKTMQRNW IGRSTGASVL FATAADDIEV FTTRPDTLFG ATYLVLAPEH DLVDTLVTDA WPDGTDERWT YGAATPREAV AAYRTDIAAK SDLERQENKT KTGVFLGAYA TNPADGKQVP IFIADYVLAG YGTGAIMAVP GGDQRDWDFA KEFGLPIIEV VTGGDISEAA YAGDGTMVNS GFLDGMDVAS AKEAIIARLE ADGRGKRRVE YKLRDWLFAR QRYWGEPFPI VYDADGRAHP LPESALPVEL PDVPDYSPVL FDPDDADSEP SPPLNKATEW VHVELDLGDG LQSYTRDTNV MPQWAGSSWY ELRYTDPHNP DEMCAKENEA YWMGPRPDEH GPEDPGGVDL YVGGVEHAVL HLLYSRFWHK VLYDLGYVSS REPYRRLVNQ GYIQAFAYTD SRGTYVPAAE VIERDGKFFW PGPDGEIEVN QEFGKIGKSL KNSVSPDEIC DNYGADTLRV YEMSMGPLEA SRPWATKDVV GAHRFLQRVW RVVIDETSGN VRVVEHEALS DETLRLLHRT IEGVREDYAA LRNNTAAAKL IEYTNHLTKE GVAARAAIEP LVLMVAPLAP HLAEELWKRL GHDTSLAHGP FPEADPQYLV EDTIEFPVQV NGKVRGKIVV AADADKAALE AAALADEKVQ AFLAGATPKK VIVVPGRLVN LVV

NT Sequence

gtgaccgaac ccgcaaccac gccgacaaca cccgatgagc agatcccgcg tcatcgctac aacgcggatc tcgcggggca gatcgagcgg gcgtggcagg agacgtggtc ggaccgcggc acgttcaacg tcgccaatcc cgtggggtca ctcgcgccga cggacggctc ggatgttcct gccgacaaga tgttcgtcca ggacatgttc ccgtacccgt cgggcgacgg tctgcacgtc gggcacccgc tcggctacat cgcgacagac gtctacgcgc gctactaccg catgctcggc cgcaacgtgt tgcacgcgct ggggttcgat gccttcggcc tgcccgccga gcagtacgcg gtgcagaccg ggacgcatcc gcgcacgcgc accgaggcca acatcgtcaa cttccgcagg cagctgggcc ggctggggct gggccacgac acgcgccgca gcttctccac caccgacgtc gactactaca agtggacgca gtggatcttc ctgcagatct acaacgcctg gttcgaccgc gaccagaaca aggcccgccg catcagcgag ctggtcgagg agttcgaatc gggtaagcgc acgctcgacg acggccgcaa ctgggccgat ctgtccaagg gtgagcgcgc cgatgtgatc gacggttacc gcttggtgta ccgcgccgac tcgatggtga actggtgccc cggtctgggc accgtgctgg ccaacgagga ggtgacctcc gagggccgca gcgaccgcgg caacttcccg gtgttccgga agcggttgcg ccagtggatg atgcgcatca ccgcgtactc cgacaggctc ctcgaggatc tcgacgtgct ggattggccc gagaaggtca agaccatgca gcgcaactgg atcggccgct cgacgggtgc ctcggtgctg ttcgcgaccg cggccgacga catcgaggtg ttcaccacgc gtcccgatac gttgttcggc gcaacgtatc tggtgttggc ccccgagcac gatctggtcg acacgctggt gaccgacgcg tggcccgacg gcaccgacga gcgctggacc tacggcgcgg cgaccccgcg cgaggccgtc gcggcctacc gcacggacat cgccgcgaag tcggatctgg agcgccagga gaacaagacc aagaccggtg tgttcctggg tgcctacgcg accaatcccg ctgacggcaa acaggttccg atcttcatcg ccgactacgt gctggccggc tacggcaccg gcgccatcat ggcggtgccc ggcggcgatc agcgtgactg ggatttcgcc aaggagttcg gcctgcccat catcgaggtg gtgaccggtg gtgacatctc ggaggccgcc tacgcgggcg acggcaccat ggtcaactcg ggcttcctcg acggcatgga tgtggcgtcg gccaaggagg ccatcatcgc ccgtctcgag gccgacggcc gcgggaagcg ccgcgtcgag tacaagctgc gtgactggct gttcgcccgc cagcgctact ggggtgagcc gttccccatt gtctacgacg ccgacggacg tgcccacccg ctgccggaat ctgctctgcc cgttgagctt ccggacgttc ccgactactc gccggtgctg ttcgacccgg acgacgccga cagcgagccc tcgccgccgc tgaacaaggc caccgaatgg gtccacgtcg aactcgacct cggtgacggt ctgcagtcct acacgcgtga caccaatgtc atgccgcagt gggccggcag ctcgtggtac gagctgcgct acaccgaccc gcacaatccg gatgagatgt gcgccaagga gaacgaggcg tactggatgg ggccgcgccc cgacgagcac ggcccggagg atccgggcgg tgtcgacctg tacgtcggcg gcgtggaaca cgctgtgctg cacctgctgt actcgcggtt ctggcacaag gtgctctacg acctgggtta cgtcagctcg cgtgagccgt accggcgcct ggtcaaccag ggctacatcc aggccttcgc ctacaccgac tcgcgcggca cctatgtgcc cgccgccgag gtgatcgagc gggacggcaa gttcttctgg cccggtcccg atggtgaaat agaggtgaat caggagttcg gcaagatcgg caagagcctg aagaactcgg tgtcgcccga cgagatctgc gacaactacg gcgccgacac gctgcgcgtg tacgagatgt cgatgggccc gctcgaggcg tcgcggccgt gggccaccaa ggacgtcgtc ggtgcgcacc gcttcctgca gcgtgtgtgg cgcgtcgtga tcgacgagac ctcgggcaac gtgcgtgtcg tcgagcacga ggcactcagc gacgagacgc tgcgcctgct gcaccgcacg atcgaaggtg tgcgcgaaga ctatgcggcc cttcgcaaca acaccgcggc cgccaagctc atcgagtaca ccaaccacct caccaaggag ggtgtggcgg cgcgcgccgc gatcgaacca ctggtgctga tggtcgcgcc gctggcgccg cacctcgccg aggaactgtg gaagcggctg ggccacgaca cctcgctggc acacggacct ttccccgagg ccgacccgca gtacctggtg gaggacacca tcgagttccc cgtccaggtc aacggcaagg tgcgcggcaa gatcgtcgtg gcggccgacg cggacaaggc cgcgctcgag gccgcggccc tggccgacga gaaggtgcag gcgttcctgg ccggggccac gcccaagaag gtcatcgtcg ttccgggccg gctggtcaac ctggtcgtct ag

Details for MysmA.17943.a.B1.GE42457

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	108kDa
OBSERVED MW:	108kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT EPATTPTTPD EQIPRHRYNA DLAGQIERAW QETWSDRGTF NVANPVGSLA PTDGSDVPAD KMFVQDMFPY PSGDGLHVGH PLGYIATDVY ARYYRMLGRN VLHALGFDAF GLPAEQYAVQ TGTHPRTRTE ANIVNFRRQL GRLGLGHDTR RSFSTTDVDY YKWTQWIFLQ IYNAWFDRDQ NKARRISELV EEFESGKRTL DDGRNWADLS KGERADVIDG YRLVYRADSM VNWCPGLGTV LANEEVTSEG RSDRGNFPVF RKRLRQWMMR ITAYSDRLLE DLDVLDWPEK VKTMQRNWIG RSTGASVLFA TAADDIEVFT TRPDTLFGAT YLVLAPEHDL VDTLVTDAWP DGTDERWTYG AATPREAVAA YRTDIAAKSD LERQENKTKT GVFLGAYATN PADGKQVPIF IADYVLAGYG TGAIMAVPGG DQRDWDFAKE FGLPIIEVVT GGDISEAAYA GDGTMVNSGF LDGMDVASAK EAIIARLEAD GRGKRRVEYK LRDWLFARQR YWGEPFPIVY DADGRAHPLP ESALPVELPD VPDYSPVLFD PDDADSEPSP PLNKATEWVH VELDLGDGLQ SYTRDTNVMP QWAGSSWYEL RYTDPHNPDE MCAKENEAYW MGPRPDEHGP EDPGGVDLYV GGVEHAVLHL LYSRFWHKVL YDLGYVSSRE PYRRLVNQGY IQAFAYTDSR GTYVPAAEVI ERDGKFFWPG PDGEIEVNQE FGKIGKSLKN SVSPDEICDN YGADTLRVYE MSMGPLEASR PWATKDVVGA HRFLQRVWRV VIDETSGNVR VVEHEALSDE TLRLLHRTIE GVREDYAALR NNTAAAKLIE YTNHLTKEGV AARAAIEPLV LMVAPLAPHL AEELWKRLGH DTSLAHGPFP EADPQYLVED TIEFPVQVNG KVRGKIVVAA DADKAALEAA ALADEKVQAF LAGATPKKVI VVPGRLVNLV V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aacccgcaac cacgccgaca acacccgatg agcagatccc gcgtcatcgc tacaacgcgg atctcgcggg gcagatcgag cgggcgtggc aggagacgtg gtcggaccgc ggcacgttca acgtcgccaa tcccgtgggg tcactcgcgc cgacggacgg ctcggatgtt cctgccgaca agatgttcgt ccaggacatg ttcccgtacc cgtcgggcga cggtctgcac gtcgggcacc cgctcggcta catcgcgaca gacgtctacg cgcgctacta ccgcatgctc ggccgcaacg tgttgcacgc gctggggttc gatgccttcg gcctgcccgc cgagcagtac gcggtgcaga ccgggacgca tccgcgcacg cgcaccgagg ccaacatcgt caacttccgc aggcagctgg gccggctggg gctgggccac gacacgcgcc gcagcttctc caccaccgac gtcgactact acaagtggac gcagtggatc ttcctgcaga tctacaacgc ctggttcgac cgcgaccaga acaaggcccg ccgcatcagc gagctggtcg aggagttcga atcgggtaag cgcacgctcg acgacggccg caactgggcc gatctgtcca agggtgagcg cgccgatgtg atcgacggtt accgcttggt gtaccgcgcc gactcgatgg tgaactggtg ccccggtctg ggcaccgtgc tggccaacga ggaggtgacc tccgagggcc gcagcgaccg cggcaacttc ccggtgttcc ggaagcggtt gcgccagtgg atgatgcgca tcaccgcgta ctccgacagg ctcctcgagg atctcgacgt gctggattgg cccgagaagg tcaagaccat gcagcgcaac tggatcggcc gctcgacggg tgcctcggtg ctgttcgcga ccgcggccga cgacatcgag gtgttcacca cgcgtcccga tacgttgttc ggcgcaacgt atctggtgtt ggcccccgag cacgatctgg tcgacacgct ggtgaccgac gcgtggcccg acggcaccga cgagcgctgg acctacggcg cggcgacccc gcgcgaggcc gtcgcggcct accgcacgga catcgccgcg aagtcggatc tggagcgcca ggagaacaag accaagaccg gtgtgttcct gggtgcctac gcgaccaatc ccgctgacgg caaacaggtt ccgatcttca tcgccgacta cgtgctggcc ggctacggca ccggcgccat catggcggtg cccggcggcg atcagcgtga ctgggatttc gccaaggagt tcggcctgcc catcatcgag gtggtgaccg gtggtgacat ctcggaggcc gcctacgcgg gcgacggcac catggtcaac tcgggcttcc tcgacggcat ggatgtggcg tcggccaagg aggccatcat cgcccgtctc gaggccgacg gccgcgggaa gcgccgcgtc gagtacaagc tgcgtgactg gctgttcgcc cgccagcgct actggggtga gccgttcccc attgtctacg acgccgacgg acgtgcccac ccgctgccgg aatctgctct gcccgttgag cttccggacg ttcccgacta ctcgccggtg ctgttcgacc cggacgacgc cgacagcgag ccctcgccgc cgctgaacaa ggccaccgaa tgggtccacg tcgaactcga cctcggtgac ggtctgcagt cctacacgcg tgacaccaat gtcatgccgc agtgggccgg cagctcgtgg tacgagctgc gctacaccga cccgcacaat ccggatgaga tgtgcgccaa ggagaacgag gcgtactgga tggggccgcg ccccgacgag cacggcccgg aggatccggg cggtgtcgac ctgtacgtcg gcggcgtgga acacgctgtg ctgcacctgc tgtactcgcg gttctggcac aaggtgctct acgacctggg ttacgtcagc tcgcgtgagc cgtaccggcg cctggtcaac cagggctaca tccaggcctt cgcctacacc gactcgcgcg gcacctatgt gcccgccgcc gaggtgatcg agcgggacgg caagttcttc tggcccggtc ccgatggtga aatagaggtg aatcaggagt tcggcaagat cggcaagagc ctgaagaact cggtgtcgcc cgacgagatc tgcgacaact acggcgccga cacgctgcgc gtgtacgaga tgtcgatggg cccgctcgag gcgtcgcggc cgtgggccac caaggacgtc gtcggtgcgc accgcttcct gcagcgtgtg tggcgcgtcg tgatcgacga gacctcgggc aacgtgcgtg tcgtcgagca cgaggcactc agcgacgaga cgctgcgcct gctgcaccgc acgatcgaag gtgtgcgcga agactatgcg gcccttcgca acaacaccgc ggccgccaag ctcatcgagt acaccaacca cctcaccaag gagggtgtgg cggcgcgcgc cgcgatcgaa ccactggtgc tgatggtcgc gccgctggcg ccgcacctcg ccgaggaact gtggaagcgg ctgggccacg acacctcgct ggcacacgga cctttccccg aggccgaccc gcagtacctg gtggaggaca ccatcgagtt ccccgtccag gtcaacggca aggtgcgcgg caagatcgtc gtggcggccg acgcggacaa ggccgcgctc gaggccgcgg ccctggccga cgagaaggtg caggcgttcc tggccggggc cacgcccaag aaggtcatcg tcgttccggg ccggctggtc aacctggtcg tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for MysmA.17943.a.B1.PW38502

PURIFICATION DATe:	7/2/2018
CONCENTRATION:	23.93mg/ml
OBSERVED MW:	108kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACCG AACCCGCAAC CACGCCGACA ACACCCGATG AGCAGATCCC GCGTCATCGC TACAACGCGG ATCTCGCGGG GCAGATCGAG CGGGCGTGGC AGGAGACGTG GTCGGACCGC GGCACGTTCA ACGTCGCCAA TCCCGTGGGG TCACTCGCGC CGACGGACGG CTCGGATGTT CCTGCCGACA AGATGTTCGT CCAGGACATG TTCCCGTACC CGTCGGGCGA CGGTCTGCAC GTCGGGCACC CGCTCGGCTA CATCGCGACA GACGTCTACG CGCGCTACTA CCGCATGCTC GGCCGCAACG TGTTGCACGC GCTGGGGTTC GATGCCTTCG GCCTGCCCGC CGAGCAGTAC GCGGTGCAGA CCGGGACGCA TCCGCGCACG CGCACCGAGG CCAACATCGT CAACTTCCGC AGGCAGCTGG GCCGGCTGGG GCTGGGCCAC GACACGCGCC GCAGCTTCTC CACCACCGAC GTCGACTACT ACAAGTGGAC GCAGTGGATC TTCCTGCAGA TCTACAACGC CTGGTTCGAC CGCGACCAGA ACAAGGCCCG CCGCATCAGC GAGCTGGTCG AGGAGTTCGA ATCGGGTAAG CGCACGCTCG ACGACGGCCG CAACTGGGCC GATCTGTCCA AGGGTGAGCG CGCCGATGTG ATCGACGGTT ACCGCTTGGT GTACCGCGCC GACTCGATGG TGAACTGGTG CCCCGGTCTG GGCACCGTGC TGGCCAACGA GGAGGTGACC TCCGAGGGCC GCAGCGACCG CGGCAACTTC CCGGTGTTCC GGAAGCGGTT GCGCCAGTGG ATGATGCGCA TCACCGCGTA CTCCGACAGG CTCCTCGAGG ATCTCGACGT GCTGGATTGG CCCGAGAAGG TCAAGACCAT GCAGCGCAAC TGGATCGGCC GCTCGACGGG TGCCTCGGTG CTGTTCGCGA CCGCGGCCGA CGACATCGAG GTGTTCACCA CGCGTCCCGA TACGTTGTTC GGCGCAACGT ATCTGGTGTT GGCCCCCGAG CACGATCTGG TCGACACGCT GGTGACCGAC GCGTGGCCCG ACGGCACCGA CGAGCGCTGG ACCTACGGCG CGGCGACCCC GCGCGAGGCC GTCGCGGCCT ACCGCACGGA CATCGCCGCG AAGTCGGATC TGGAGCGCCA GGAGAACAAG ACCAAGACCG GTGTGTTCCT GGGTGCCTAC GCGACCAATC CCGCTGACGG CAAACAGGTT CCGATCTTCA TCGCCGACTA CGTGCTGGCC GGCTACGGCA CCGGCGCCAT CATGGCGGTG CCCGGCGGCG ATCAGCGTGA CTGGGATTTC GCCAAGGAGT TCGGCCTGCC CATCATCGAG GTGGTGACCG GTGGTGACAT CTCGGAGGCC GCCTACGCGG GCGACGGCAC CATGGTCAAC TCGGGCTTCC TCGACGGCAT GGATGTGGCG TCGGCCAAGG AGGCCATCAT CGCCCGTCTC GAGGCCGACG GCCGCGGGAA GCGCCGCGTC GAGTACAAGC TGCGTGACTG GCTGTTCGCC CGCCAGCGCT ACTGGGGTGA GCCGTTCCCC ATTGTCTACG ACGCCGACGG ACGTGCCCAC CCGCTGCCGG AATCTGCTCT GCCCGTTGAG CTTCCGGACG TTCCCGACTA CTCGCCGGTG CTGTTCGACC CGGACGACGC CGACAGCGAG CCCTCGCCGC CGCTGAACAA GGCCACCGAA TGGGTCCACG TCGAACTCGA CCTCGGTGAC GGTCTGCAGT CCTACACGCG TGACACCAAT GTCATGCCGC AGTGGGCCGG CAGCTCGTGG TACGAGCTGC GCTACACCGA CCCGCACAAT CCGGATGAGA TGTGCGCCAA GGAGAACGAG GCGTACTGGA TGGGGCCGCG CCCCGACGAG CACGGCCCGG AGGATCCGGG CGGTGTCGAC CTGTACGTCG GCGGCGTGGA ACACGCTGTG CTGCACCTGC TGTACTCGCG GTTCTGGCAC AAGGTGCTCT ACGACCTGGG TTACGTCAGC TCGCGTGAGC CGTACCGGCG CCTGGTCAAC CAGGGCTACA TCCAGGCCTT CGCCTACACC GACTCGCGCG GCACCTATGT GCCCGCCGCC GAGGTGATCG AGCGGGACGG CAAGTTCTTC TGGCCCGGTC CCGATGGTGA AATAGAGGTG AATCAGGAGT TCGGCAAGAT CGGCAAGAGC CTGAAGAACT CGGTGTCGCC CGACGAGATC TGCGACAACT ACGGCGCCGA CACGCTGCGC GTGTACGAGA TGTCGATGGG CCCGCTCGAG GCGTCGCGGC CGTGGGCCAC CAAGGACGTC GTCGGTGCGC ACCGCTTCCT GCAGCGTGTG TGGCGCGTCG TGATCGACGA GACCTCGGGC AACGTGCGTG TCGTCGAGCA CGAGGCACTC AGCGACGAGA CGCTGCGCCT GCTGCACCGC ACGATCGAAG GTGTGCGCGA AGACTATGCG GCCCTTCGCA ACAACACCGC GGCCGCCAAG CTCATCGAGT ACACCAACCA CCTCACCAAG GAGGGTGTGG CGGCGCGCGC CGCGATCGAA CCACTGGTGC TGATGGTCGC GCCGCTGGCG CCGCACCTCG CCGAGGAACT GTGGAAGCGG CTGGGCCACG ACACCTCGCT GGCACACGGA CCTTTCCCCG AGGCCGACCC GCAGTACCTG GTGGAGGACA CCATCGAGTT CCCCGTCCAG GTCAACGGCA AGGTGCGCGG CAAGATCGTC GTGGCGGCCG ACGCGGACAA GGCCGCGCTC GAGGCCGCGG CCCTGGCCGA CGAGAAGGTG CAGGCGTTCC TGGCCGGGGC CACGCCCAAG AAGGTCATCG TCGTTCCGGG CCGGCTGGTC AACCTGGTCG TCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT