MysmA.17818.a

Serine/threonine protein kinase PknA (Rv0015c ortholog)

CENTER ID: MysmA.17818.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.17818.a.B2.GE37629 soluble domain 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.28
RefSeq: YP_006564810.1
RefSeq: YP_884450.1
UniProt: A0QNG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPRVGVTLS GRYRLQRLIA TGGMGQVWEA VDSRLGRRVA VKVLKAEFST DAEFVERFRA EARTVAMLNH PGIASVYDYG ETELDGEGRT AYLVMELING EPLNSVLKRT GRLSLRHALD MLEQTGRALQ VAHTAGLVHR DVKPGNILIT PTGQVKLTDF GIAKAVDAAP VTQTGMVMGT AQYIAPEQAL GHDATAASDV YSLGVVGYES VSGKRPFTGD GALTVAMKHI KETPPPLPAD LPPNVRELIE ITLVKNPGMR YKSGGPFADA VAAVRAGRRP PRPNQAPTLG RAAPAAVPSA AQARASADLT GRAPVTAARA RPTATAAHRT PPPRRTFSSG QRALLWAAGV LGALAIVIAI LIVLNAQDRK DREQSPPPTV TDTVTETTPY EETPAAMPDL MIMLRAAQPE PPPSEQIQR
NT Sequence
atgagcccgc gcgttggagt cacgctgtcc ggtcgttacc ggctgcagcg gctgatcgcc acgggcggta tgggccaggt gtgggaggcc gtggattcgc ggctgggccg ccgggtcgcc gtcaaggtgc tcaaggccga gttctccacc gacgccgagt tcgtcgaacg cttccgggcc gaggcccgca ccgtcgcgat gctcaaccat cccggcatcg cgagcgtgta cgactacggc gagaccgaac tcgacggcga gggccgcacc gcgtacctgg tgatggagct catcaacggt gagccgctga actcggtgct caagcgcacc ggccggctgt cgctgcggca cgcgctggac atgctggagc agaccggccg tgcgctgcag gtcgcgcaca ccgcgggcct ggtgcaccgc gacgtcaagc ccggcaacat cctcatcacc ccgacggggc aggtgaagct caccgacttc ggcatcgcca aggccgtcga cgccgcgccg gtcacccaga ccggcatggt gatgggcacc gcgcagtaca tcgcgcccga gcaggccctg ggccatgacg cgaccgccgc cagtgacgtg tattcgctgg gcgtcgttgg ctacgaatcg gtttcgggca agcgcccgtt caccggcgac ggtgcgctga cggtcgcaat gaagcacatc aaggagaccc cgccgccgct tccggccgat ctgccgccga acgtgcgcga gctgatcgag atcacgctcg tgaagaaccc gggcatgcgg tacaagtcgg gcggcccgtt cgccgacgcg gtcgccgcgg tgcgcgcggg tcgcaggcca ccgcggccca accaggcacc gacgctggga cgtgccgcac cggccgcggt gccgtcggcc gcgcaggccc gtgcctctgc cgatctcacg ggccgtgcgc ccgtcaccgc cgcacgcgcg agacccaccg cgacggccgc gcatcggact ccgccgcctc gacgcacgtt ctcctcgggg caacgggcgc tattgtgggc ggctggcgtc ctcggcgcgt tggccatcgt gatcgcgatc ctgatcgtgc tcaacgcgca ggaccgcaag gaccgcgagc agtcgccacc gccgacggtc accgacaccg tcaccgagac gacgccctac gaggagaccc cggcggcgat gcctgacctc atgatcatgt tgcgtgcggc tcagcccgag ccgccgccat ccgaacagat acagcgatga
Details for MysmA.17818.a.B2.GE37629
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/13/2014
EXPECTED MW: 37kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MSPRVGVTLS GRYRLQRLIA TGGMGQVWEA VDSRLGRRVA VKVLKAEFST DAEFVERFRA EARTVAMLNH PGIASVYDYG ETELDGEGRT AYLVMELING EPLNSVLKRT GRLSLRHALD MLEQTGRALQ VAHTAGLVHR DVKPGNILIT PTGQVKLTDF GIAKAVDAAP VTQTGMVMGT AQYIAPEQAL GHDATAASDV YSLGVVGYES VSGKRPFTGD GALTVAMKHI KETPPPLPAD LPPNVRELIE ITLVKNPGMR YKSGGPFADA VAAVRAGRRP PRPNQAPTLG RAAPAAVPSA AQARASADLT GRAPVTAARA RPTATAAHRT PPPRRTFSSG QR
Validated NT Sequence
atcttactca cttaccgttg ccccgaggag aacgtgcgtc gaggcggcgg agtccgatgc gcggccgtcg cggtgggtct cgcgcgtgcg gcggtgacgg gcgcacggcc cgtgagatcg gcagaggcac gggcctgcgc ggccgacggc accgcggccg gtgcggcacg tcccagcgtc ggtgcctggt tgggccgcgg tggcctgcga cccgcgcgca ccgcggcgac cgcgtcggcg aacgggccgc ccgacttgta ccgcatgccc gggttcttca cgagcgtgat ctcgatcagc tcgcgcacgt tcggcggcag atcggccgga agcggcggcg gggtctcctt gatgtgcttc attgcgaccg tcagcgcacc gtcgccggtg aacgggcgct tgcccgaaac cgattcgtag ccaacgacgc ccagcgaata cacgtcactg gcggcggtcg cgtcatggcc cagggcctgc tcgggcgcga tgtactgcgc ggtgcccatc accatgccgg tctgggtgac cggcgcggcg tcgacggcct tggcgatgcc gaagtcggtg agcttcacct gccccgtcgg ggtgatgagg atgttgccgg gcttgacgtc gcggtgcacc aggcccgcgg tgtgcgcgac ctgcagcgca cggccggtct gctccagcat gtccagcgcg tgccgcagcg acagccggcc ggtgcgcttg agcaccgagt tcagcggctc accgttgatg agctccatca ccaggtacgc ggtgcggccc tcgccgtcga gttcggtctc gccgtagtcg tacacgctcg cgatgccggg atggttgagc atcgcgacgg tgcgggcctc ggcccggaag cgttcgacga actcggcgtc ggtggagaac tcggccttga gcaccttgac ggcgacccgg cggcccagcc gcgaatccac ggcctcccac acctggccca taccgcccgt ggcgatcagc cgctgcagcc ggtaacgacc ggacagcgtg actccaacgc gcgggctcat atggtggtgg tggtggtgag cc
Expected Protein Sequence
MAHHHHHHMS PRVGVTLSGR YRLQRLIATG GMGQVWEAVD SRLGRRVAVK VLKAEFSTDA EFVERFRAEA RTVAMLNHPG IASVYDYGET ELDGEGRTAY LVMELINGEP LNSVLKRTGR LSLRHALDML EQTGRALQVA HTAGLVHRDV KPGNILITPT GQVKLTDFGI AKAVDAAPVT QTGMVMGTAQ YIAPEQALGH DATAASDVYS LGVVGYESVS GKRPFTGDGA LTVAMKHIKE TPPPLPADLP PNVRELIEIT LVKNPGMRYK SGGPFADAVA AVRAGRRPPR PNQAPTLGRA APAAVPSAAQ ARASADLTGR APVTAARARP TATAAHRTPP PRRTFSSGQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc cgcgcgttgg agtcacgctg tccggtcgtt accggctgca gcggctgatc gccacgggcg gtatgggcca ggtgtgggag gccgtggatt cgcggctggg ccgccgggtc gccgtcaagg tgctcaaggc cgagttctcc accgacgccg agttcgtcga acgcttccgg gccgaggccc gcaccgtcgc gatgctcaac catcccggca tcgcgagcgt gtacgactac ggcgagaccg aactcgacgg cgagggccgc accgcgtacc tggtgatgga gctcatcaac ggtgagccgc tgaactcggt gctcaagcgc accggccggc tgtcgctgcg gcacgcgctg gacatgctgg agcagaccgg ccgtgcgctg caggtcgcgc acaccgcggg cctggtgcac cgcgacgtca agcccggcaa catcctcatc accccgacgg ggcaggtgaa gctcaccgac ttcggcatcg ccaaggccgt cgacgccgcg ccggtcaccc agaccggcat ggtgatgggc accgcgcagt acatcgcgcc cgagcaggcc ctgggccatg acgcgaccgc cgccagtgac gtgtattcgc tgggcgtcgt tggctacgaa tcggtttcgg gcaagcgccc gttcaccggc gacggtgcgc tgacggtcgc aatgaagcac atcaaggaga ccccgccgcc gcttccggcc gatctgccgc cgaacgtgcg cgagctgatc gagatcacgc tcgtgaagaa cccgggcatg cggtacaagt cgggcggccc gttcgccgac gcggtcgccg cggtgcgcgc gggtcgcagg ccaccgcggc ccaaccaggc accgacgctg ggacgtgccg caccggccgc ggtgccgtcg gccgcgcagg cccgtgcctc tgccgatctc acgggccgtg cgcccgtcac cgccgcacgc gcgagaccca ccgcgacggc cgcgcatcgg actccgccgc ctcgacgcac gttctcctcg gggcaacggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat