SSGCID: Target Page

MysmA.17716.a

Urease subunit alpha

CENTER ID:	MysmA.17716.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17716.a.B1.GE40214	Full length(MysmA.17716.a)		1	577

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3573
UniProt:	A0QYE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTALSRSNYA ALFGPTTGDR IRLADTDLLI EITEDRSGGP ELAGDEAVFG GGKVLRESMG QGRATRADGA PDTVITGAVI LDHWGIIKAD IGIRDGRIVA IGKAGNPDIM SGVHPDLVVG PSTEIIAGNG RILTAGAIDC HVHLICPQIM DEALGGGITT IIGGGTGPAE GTKATTVTPG AWHLARMLES LDSWPMNIVL LGKGNTVSAD SMWEQLRGGA AGFKLHEDWG TTPAAIDACL TVCDAAGVQA AIHTDTLNEM GFVEDTLAAI GGRSIHAYHT EGAGGGHAPD IITVAGHPNV LPSSTNPTRP HTVNTLDEHL DMLMVCHHLK PSVPEDLAFA ESRIRPSTIA AEDLLHDIGA ISMIGSDAQA MGRIGEVVMR TWQTAHVMKR RRGALEGDGS GKNGADNLRA RRYVAKYTIC PAVAHGLDHE IGSVEVGKLA DLVLWEPAFF GVRPHAVIKG GMIAWAAMGD ANASIPTPQP VLPRPMFGAA PAAAAATSVH FVAPQAIEDG LAERIDVRRK LVAVKDVRKV GKAQMPLNDA LPRIEVEPDT FTVRIDGEVW DEQPAAELPM AQRYFLF

NT Sequence

ATGACCGCAC TGTCACGGAG CAACTACGCC GCGCTGTTCG GTCCCACCAC CGGGGACCGC ATCCGGCTGG CCGACACCGA CCTGCTGATC GAGATCACCG AGGACCGCAG CGGCGGACCG GAGCTCGCCG GTGACGAGGC CGTGTTCGGC GGCGGCAAGG TGTTGCGCGA GTCCATGGGG CAGGGCCGGG CCACCCGCGC CGACGGCGCC CCCGACACCG TCATCACCGG AGCGGTGATC CTCGACCACT GGGGGATCAT CAAGGCCGAC ATCGGGATCC GCGACGGCCG GATCGTTGCG ATCGGCAAGG CGGGCAACCC CGACATCATG TCGGGCGTGC ACCCCGATCT GGTGGTCGGC CCGTCCACCG AGATCATCGC GGGCAACGGT CGCATCCTCA CCGCGGGTGC CATCGACTGC CACGTGCACC TGATCTGCCC GCAGATCATG GACGAGGCGC TCGGCGGCGG CATCACCACG ATCATCGGCG GCGGCACCGG CCCGGCCGAG GGCACCAAGG CCACCACGGT CACGCCGGGC GCATGGCATC TGGCGCGCAT GCTCGAGTCC CTGGACTCCT GGCCCATGAA CATCGTGCTG CTGGGCAAGG GCAACACCGT CAGCGCCGAC TCGATGTGGG AGCAGTTGCG CGGCGGCGCA GCGGGTTTCA AGCTCCACGA GGATTGGGGC ACCACCCCGG CGGCCATCGA CGCGTGCCTT ACGGTGTGCG ACGCCGCAGG CGTGCAGGCG GCCATCCACA CCGACACGCT CAACGAGATG GGCTTCGTCG AGGACACCCT GGCCGCCATC GGCGGCCGCT CGATCCACGC CTACCACACC GAGGGTGCCG GCGGCGGGCA CGCGCCGGAC ATCATCACGG TGGCGGGCCA TCCCAACGTG CTGCCCAGCT CCACCAACCC GACCCGGCCG CACACCGTCA ACACCCTCGA CGAGCACCTG GACATGCTGA TGGTCTGCCA TCACCTGAAA CCCAGTGTGC CCGAGGATCT CGCGTTCGCC GAGAGCCGTA TCAGGCCGTC GACCATCGCG GCCGAGGACC TGCTGCACGA CATCGGGGCC ATCTCGATGA TCGGCAGTGA CGCGCAGGCC ATGGGCCGCA TCGGCGAGGT CGTGATGCGC ACCTGGCAGA CCGCGCACGT GATGAAGCGC CGCCGCGGCG CGCTGGAAGG CGACGGCTCT GGGAAGAACG GCGCGGACAA CCTGCGCGCG CGCCGCTACG TCGCCAAGTA CACGATCTGT CCCGCGGTCG CGCACGGCCT CGACCACGAG ATCGGCTCGG TCGAGGTCGG CAAGCTCGCG GATCTGGTGC TGTGGGAGCC CGCGTTCTTC GGGGTGCGCC CGCATGCGGT GATCAAGGGC GGCATGATCG CGTGGGCCGC GATGGGCGAC GCCAACGCGT CGATCCCGAC ACCGCAGCCC GTGCTGCCGC GGCCCATGTT CGGAGCCGCA CCGGCCGCAG CGGCCGCGAC CTCGGTGCAC TTCGTGGCGC CGCAGGCCAT CGAGGACGGC CTGGCCGAAC GCATCGACGT CAGGCGGAAA CTCGTCGCGG TCAAGGACGT CCGCAAGGTC GGCAAGGCGC AGATGCCGCT CAACGACGCG CTGCCACGCA TCGAGGTCGA ACCCGACACG TTCACGGTGC GCATCGACGG CGAGGTGTGG GACGAACAAC CCGCCGCCGA GCTACCCATG GCACAGCGAT ACTTCCTGTT C

Details for MysmA.17716.a.B1.GE40214

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/24/2016
EXPECTED MW:	62kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	84
PERCENT COVERAGE:	56

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgacc gcactgtcac ggagcaacta cgccgcgctg ttcggtccca ccaccgggga ccgcatccgg ctggccgaca ccgacctgct gatcgagatc accgaggacc gcagcggcgg accggagctc gccggtgacg aggccgtgtt cggcggcggc aaggtgttgc gcgagtccat ggggcagggc cgggccaccc gcgccgacgg cgcccccgac accgtcatca ccggagcggt gatcctcgac cactggggga tcatcaaggc cgacatcggg atccgcgacg gccggatcgt tgcgatcggc aaggcgggca accccgacat catgtcgggc gtgcaccccg atctggtggt cggcccgtcc accgagatca tcgcgggcaa cggtcgcatc ctcaccgcgg gtgccatcga ctgccacgtg cacctgatct gcccgcagat catggacgag gcgctcggcg gcggcatcac cacgatcatc ggcggcggca ccggcccggc cgagggcacc aaggccacca cggtcacgcc gggcgcatgg catctggcgc gcatgctcga gtccctggac tcctggccca tgaacatcgt gctgctgggc aagggcaaca ccgtcagcgc cgactcgatg tgggagcagt tgcgcggcgg cgcagcgggt ttcaagctcc acgaggattg gggcaccacc ccggcggcca tcgacgcgtg ccttacggtg tgcgacgccg nnnnngtgca ggcggccann nanancgaca nnctcannga gatggnnnnn gtcnnggaca cccnngcnnc catcggcggc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnnn gccnnnnatc atcannnnnn nngggccatc ccaacgtgct gcccagctcc accaacccga ccnnnnnngc acaccgtcaa cacccncgac gagcacctgg acatgctgat ggtctgccat cacctgaaac ccagtgtgcc cgaggatctc gcgttcgccg agagccgtat caggccgtcg accatcgcgg ccgaggacct gctgcacgac atcggggcca tctcgatgat cggcagtgac gcgcaggcca tgggccgcat cggcgaggtc gtgatgcgca cctggcagac cgcgcacgtg atgaagcgcc gccgcggcgc gctggaaggc gacggctctg ggaagaacgg cgcggacaac ctgcgcgcgc gccgctacgt cgccaagtac acgatctgtc ccgcggtcgc gcacggcctc gaccacgaga tcggctcggt cgaggtcggc aagctcgcgg atctggtgct gtgggagccc gcgttcttcg gggtgcgccc gcatgcggtg atcaagggcg gcatgatcgc gtgggccgcg atgggcgacg ccaacgcgtc gatcccgaca ccgcagcccg tgctgccgcg gcccatgttc ggagccgcac cggccgcagc ggccgcgacc tcggtgcact tcgtggcgcc gcaggccatc gaggacggcc tggccgaacg catcgacgtc aggcggaaac tcgtcgcggt caaggacgtc cgcaaggtcg gcaaggcgca gatgccgctc aacgacgcgc tgccacgcat cgaggtcgaa cccgacacgt tcacggtgcg catcgacggc gaggtgtggg acgaacaacc cgccgccgag ctac

Expected Protein Sequence

MAHHHHHHMT ALSRSNYAAL FGPTTGDRIR LADTDLLIEI TEDRSGGPEL AGDEAVFGGG KVLRESMGQG RATRADGAPD TVITGAVILD HWGIIKADIG IRDGRIVAIG KAGNPDIMSG VHPDLVVGPS TEIIAGNGRI LTAGAIDCHV HLICPQIMDE ALGGGITTII GGGTGPAEGT KATTVTPGAW HLARMLESLD SWPMNIVLLG KGNTVSADSM WEQLRGGAAG FKLHEDWGTT PAAIDACLTV CDAAGVQAAI HTDTLNEMGF VEDTLAAIGG RSIHAYHTEG AGGGHAPDII TVAGHPNVLP SSTNPTRPHT VNTLDEHLDM LMVCHHLKPS VPEDLAFAES RIRPSTIAAE DLLHDIGAIS MIGSDAQAMG RIGEVVMRTW QTAHVMKRRR GALEGDGSGK NGADNLRARR YVAKYTICPA VAHGLDHEIG SVEVGKLADL VLWEPAFFGV RPHAVIKGGM IAWAAMGDAN ASIPTPQPVL PRPMFGAAPA AAAATSVHFV APQAIEDGLA ERIDVRRKLV AVKDVRKVGK AQMPLNDALP RIEVEPDTFT VRIDGEVWDE QPAAELPMAQ RYFLF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cactgtcacg gagcaactac gccgcgctgt tcggtcccac caccggggac cgcatccggc tggccgacac cgacctgctg atcgagatca ccgaggaccg cagcggcgga ccggagctcg ccggtgacga ggccgtgttc ggcggcggca aggtgttgcg cgagtccatg gggcagggcc gggccacccg cgccgacggc gcccccgaca ccgtcatcac cggagcggtg atcctcgacc actgggggat catcaaggcc gacatcggga tccgcgacgg ccggatcgtt gcgatcggca aggcgggcaa ccccgacatc atgtcgggcg tgcaccccga tctggtggtc ggcccgtcca ccgagatcat cgcgggcaac ggtcgcatcc tcaccgcggg tgccatcgac tgccacgtgc acctgatctg cccgcagatc atggacgagg cgctcggcgg cggcatcacc acgatcatcg gcggcggcac cggcccggcc gagggcacca aggccaccac ggtcacgccg ggcgcatggc atctggcgcg catgctcgag tccctggact cctggcccat gaacatcgtg ctgctgggca agggcaacac cgtcagcgcc gactcgatgt gggagcagtt gcgcggcggc gcagcgggtt tcaagctcca cgaggattgg ggcaccaccc cggcggccat cgacgcgtgc cttacggtgt gcgacgccgc aggcgtgcag gcggccatcc acaccgacac gctcaacgag atgggcttcg tcgaggacac cctggccgcc atcggcggcc gctcgatcca cgcctaccac accgagggtg ccggcggcgg gcacgcgccg gacatcatca cggtggcggg ccatcccaac gtgctgccca gctccaccaa cccgacccgg ccgcacaccg tcaacaccct cgacgagcac ctggacatgc tgatggtctg ccatcacctg aaacccagtg tgcccgagga tctcgcgttc gccgagagcc gtatcaggcc gtcgaccatc gcggccgagg acctgctgca cgacatcggg gccatctcga tgatcggcag tgacgcgcag gccatgggcc gcatcggcga ggtcgtgatg cgcacctggc agaccgcgca cgtgatgaag cgccgccgcg gcgcgctgga aggcgacggc tctgggaaga acggcgcgga caacctgcgc gcgcgccgct acgtcgccaa gtacacgatc tgtcccgcgg tcgcgcacgg cctcgaccac gagatcggct cggtcgaggt cggcaagctc gcggatctgg tgctgtggga gcccgcgttc ttcggggtgc gcccgcatgc ggtgatcaag ggcggcatga tcgcgtgggc cgcgatgggc gacgccaacg cgtcgatccc gacaccgcag cccgtgctgc cgcggcccat gttcggagcc gcaccggccg cagcggccgc gacctcggtg cacttcgtgg cgccgcaggc catcgaggac ggcctggccg aacgcatcga cgtcaggcgg aaactcgtcg cggtcaagga cgtccgcaag gtcggcaagg cgcagatgcc gctcaacgac gcgctgccac gcatcgaggt cgaacccgac acgttcacgg tgcgcatcga cggcgaggtg tgggacgaac aacccgccgc cgagctaccc atggcacagc gatacttcct gttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat