MysmA.17261.a

recB Exodeoxyribonuclease V, beta subunit (EC 3.1.11.5)

CENTER ID: MysmA.17261.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.17261.a.A1.GE30128 full length 1 1083
MysmA.17261.a.A2.GE30129 UvrD-like helicase 1 323
MysmA.17261.a.A3.GE30130 UvrD-like helicase C-terminal domain 349 607
MysmA.17261.a.A4.GE30131 Phage-type/RecB exonuclease C-terminal 754 1081
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.17261.a.A2.PS00802 UvrD-like helicase 1 323
MysmA.17261.a.A4.PS00845 Phage-type/RecB exonuclease C-terminal 754 1081
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_140510
PATRIC ID: fig|246196.19.peg.1314
RefSeq: YP_885717.1
UniProt: A0QS29
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVFDLLGPL PAPNTTTVLE ASAGTGKTFA LAGLVTRFVA EGVATLDQML LITFGRAASQ ELRERVRAQI VAALVALDDP SRACNDLEEY LVKTDQQARR RRLRDALAGF DAATIATTHQ FCQIVLKSLG VAGDSDAGVT LVESLDDLVS EIVDDLYLAH FGGQKDDPEL SYPEALKLAR VVVGNPATQL RPRDPDPDSP AAVRLKFARD VLAELEIRKR RRGVLGYDDL LTRLADALEP EDSPARVRMQ QRWPIVMVDE FQDTDPVQWQ VIERAFSGRS TLVLIGDPKQ AIYAFRGGDI ATYLRAAATA GDKQTLGTNW RSDRALVDRL QAVLRGAQLG GPDIVVHDVQ ARHQGHRLVG APRNDPFRLR VVSRKPGNTR VIPIDQLRRH IGRDLAADIS ALLNSGATWC DQPVQAKDIA VITETHKDAR ACHAALLAAG IPAVYTGDSD VFTSEAAEDW LYLLEAFDQP HRPGLVRAAA ATMFFGETAE SLAAGGDALT DRVADTLREW AGHARERGVA AIFEAAQLAG MGKRVLSWQG GERLMTDLAH MTQLLQDTAH REGFGLAALR DWLRTQRSER GGESERNRRL DSDAAAVQIM TVWVSKGLQF PVVYLPFAFN RYVPEPDLVL FHDDGQRCLH VGGADPAVAR AGRAEAAGDD SRLTYVALTR AQSQVVAWWA PSYDEPNGGL SRLMRGRAPG EAIVPDKCSP PKISDEDALE RLRAWEAAGG PVIEESVIGA VSPVPPEPAP EDLAARKFFR AIDMAWKRTS YSGLLRAAET AGVGVSSEPE VTERDDEFDD IPVVAPAEGA DVPSPMAHLP TGAAFGSLVH AVLETADPFA EDLTAELATH IDAHSQHWPV EVETAELAAA LVPMHDTPLG PLAPGLTLRQ IGLRDRLCEL DFEFPMAGGD LRGGRFARLS DVGELLREYL PADDPLAVYA ERLSTGILGV QPLRGYLSGS VDAVLRVGEK FVIVDYKTNW LGTGDGTLTA ADYGRRRMVE AMLHSDYPLQ ALLYAVVLHR YLGWRLSGYD PATHLGGVLY LFVRGMCGAG TPVVDGHPAG VFSWEPPADL VVALSKLLDA EAP
NT Sequence
gtgaaggttt tcgatctgct cggcccgctg cccgcaccga acaccaccac ggtgctggag gccagcgcgg gcaccggcaa gacgttcgcg ctggccggtc tggtcactcg ttttgtcgcc gagggcgtcg cgacgctcga ccagatgctg ctcatcacgt tcggccgcgc cgccagccag gaactgcgcg agcgtgtgcg cgcccagatc gtcgccgcgc tcgtcgcgtt ggacgacccg tcgcgggcct gcaacgacct ggaggaatac ctggtcaaga ccgaccagca ggcgcggcgc cggcgcctgc gcgacgcgct ggccgggttc gacgccgcca ccatcgcgac cacgcaccag ttctgccaga tcgtgctgaa atctcttggc gtggctgggg actccgacgc cggggtgacc ctcgtggaga gcctcgacga cttggtctcc gagatcgtcg acgatctgta tctcgcgcac ttcggcgggc agaaggacga tcccgagctc agctatcccg aggccctcaa gctcgcgcgc gtcgtcgtcg gcaacccggc cacccaactg cgcccgcgcg acccggaccc cgattccccc gccgcggtgc gcctgaagtt cgcgcgggat gtgctcgccg agttggagat ccgcaagcgt cggcgcggcg tgctcggcta cgacgatctg ctgacccggc tggccgatgc actcgaaccc gaggactccc cggcgcgcgt gcgcatgcag cagcgctggc cgatcgtgat ggtcgacgag ttccaggaca ccgatcccgt gcagtggcag gtgatcgagc gcgcgttctc ggggcggtcg acgctggtgc tcatcggtga tcccaagcag gcgatctacg cgttccgcgg tggcgacatc gcgacgtacc tgcgggccgc cgcgacggcc ggtgacaagc agacgctggg caccaactgg cgcagcgacc gcgcactggt ggaccgcctg caggccgtgc tgcgcggcgc acaactcggc ggacccgaca tcgtggtgca cgacgtgcag gcacgccatc aggggcaccg cctggtgggc gcaccgcgca acgacccgtt ccggctgcgc gtggtgtcac gaaaacccgg caacaccagg gtgatcccga tcgatcagct gcgccgccac atcggccggg atctcgcggc cgacatctct gccctgctga acagcggggc cacgtggtgc gaccaaccgg tccaggcgaa ggacatcgcg gtcatcaccg agacccacaa ggacgccagg gcgtgtcatg cggcgctgct cgcggcgggc attcccgcgg tctacaccgg ggattcggat gtgttcacct ccgaggccgc cgaggactgg ctgtacctgc tcgaggcgtt cgaccagccg caccggccgg gtctggtgcg cgccgcggcc gccaccatgt tcttcggcga gaccgcggaa agccttgccg cgggcggcga tgcgctcacc gaccgggtcg ccgacacgct gcgtgaatgg gcggggcatg cccgtgagcg cggtgtcgcc gcgatcttcg aggccgcgca actcgccggt atgggcaagc gtgtgctgtc gtggcagggc ggtgaacggt tgatgacgga tctcgcccac atgacccagt tgctgcagga caccgcacac cgcgagggct tcgggctggc cgcgctgcgc gactggctgc gcacgcagcg ctcggagagg ggcggcgaat cggaacgcaa ccgtcgtctc gacagtgacg ccgcggccgt gcagatcatg accgtgtggg tgagcaaggg tctgcagttc ccggtcgtct acctgccgtt cgcgttcaat cgctatgtgc ccgagccgga tctggtgttg ttccacgacg acgggcagcg ctgcctgcat gtcggcggag ccgatccggc ggtcgcccgc gcggggcgtg ccgaggccgc gggcgacgac agccgtctca cctacgtcgc actgacccgc gcccagtcgc aggtggtggc gtggtgggca ccgtcgtacg acgaacccaa cggcggcctg tcgcggctga tgcgcggccg cgctcccggg gaggcgatcg tccccgacaa gtgctcacca cccaagatct ccgatgagga tgcgctggaa cggttgcggg cgtgggaggc agccggtggg ccggtgatcg aggaatcggt gatcggtgcg gtcagcccgg tgccacccga gcccgcgccc gaggatctgg cggctcgcaa gttctttcgc gcgatcgaca tggcgtggaa gcgcacgtcc tattcggggc tcttgcgcgc ggcagagacc gcgggtgtcg gggtgtcgag cgaacccgag gtcaccgaac gcgacgacga gttcgacgac attcccgtgg tcgccccggc cgagggtgcc gacgtgccct ctcccatggc acacctgccg acgggcgcgg cgttcggttc gctcgtgcac gccgtgctgg agaccgccga tccgttcgcc gaggatctga ccgccgaact cgcgacgcac atcgacgccc actcccagca ctggccggtc gaggtcgaga cggccgaact cgcggccgcg ctcgtgccca tgcacgacac cccgctgggg ccgttggcgc cgggtctcac gctgcggcag atcggtctac gggaccggtt gtgtgagttg gatttcgagt ttcccatggc cggtggcgat ctgcgtggtg gacggttcgc acggttgtcg gacgtcggtg agctgctgcg tgagtacctg cccgccgacg acccgctggc ggtgtacgcc gagcggttgt ccacgggcat cctcggggtg cagccgctgc gcgggtatct gtcggggtcg gtcgacgcgg tgctgcgggt cggcgagaag ttcgtcatcg tcgactacaa gacgaactgg ctgggcaccg gcgacggcac gttgaccgcg gccgactacg ggcgccgccg catggtcgag gccatgttgc attccgacta tccgctgcag gccctgctgt atgcggttgt gctgcaccgg tacctgggct ggcggctgtc gggctacgac ccggcgacgc atctcggtgg tgtgctgtat ctgttcgtac gtggcatgtg cggggccggg acgccggtcg tcgacgggca cccagccggg gtgttcagct gggaaccgcc tgccgatctg gtcgtcgcat tgtcgaagct gttggatgcg gaggcgccgt ga
Details for MysmA.17261.a.A1.GE30128
HARVESTED ON: 8/15/2010
SEQUENCED ON: 4/11/2013
EXPECTED MW: 119kDa
OBSERVED MW: 120kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 34
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEXSXXRVR MQQRWPIVMV DEFQDTDPVQ WXXSSARSXG GRRWCSSVIX SXXSTRXGXX XTXXXXXDXX XXXXLGXXWX XXXXXXXXAX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtnnnn nttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgaa ggttttcgat ctgctcggcc cgctgcccgc accgaacacc accacggtgc tggaggccag cgcgggcacc ggcaagacgt tcgcgctggc cggtctggtc actcgttttg tcgccgaggg cgtcgcgacg ctcgaccaga tgctgctcat cacgttcggc cgcgccgcca gccaggaact gcgcgagcgt gtgcgcgccc agatcgtcgc cgcgctcgtc gcgttggacg acccgtcgcg ggcctgcaac gacctggagg aatacctggt caagaccgac cagcaggcgc ggcgccggcg cctgcgcgac gcgctggccg ggttcgacgc cgccaccatc gcgaccacgc accagttctg ccagatcgtg ctgaaatctc ttggcgtggc tggggactcc gacgccgggg tgaccctcgt ggagagcctc gacgacttgg tctccgagat cgtcgacgat ctgtatctcg cgcacttcgg cgggcagaag gacgatcccg agctcagcta tcccgaggcc ctcaagctcg cgcgcgtcgt cgtcggcaac ccggccaccc aactgcgccc gcgcgacccg gaccccgatt cccccgccgc ggtgcgcctg aagttcgcgc gggatgtgct cgccgagttg gagatccgca agcgtcggcg cggcgtgctc ggctacgacg atctgctgac ccggctggcc gatgcactcg aacccgagna ctcccnnnnn cgcgtgcgca tgcagcagcg ctggccgatc gtgatggtcg acgagttcca ggacaccgat cccgtgcagt ggcnnngatc gagcgcgcgt tctcngggcg gtcgacgctg gtgctcatcg gtgatccnna gcngnngatc tacgcgtngn ggcnacntcg ngacntncng cnggnnnnnn gacngnnngn nanancanan gctgggnnnc nnntggnnnn ncnncnnnnn nntgnnnnnc nnngcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncnnnn nnnnngnnnn nnnnnnnnnn nnnnnnnnnn nnnnngnnnn nnngnnnnnn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEDSPARVR MQQRWPIVMV DEFQDTDPVQ WQVIERAFSG RSTLVLIGDP KQAIYAFRGG DIATYLRAAA TAGDKQTLGT NWRSDRALVD RLQAVLRGAQ LGGPDIVVHD VQARHQGHRL VGAPRNDPFR LRVVSRKPGN TRVIPIDQLR RHIGRDLAAD ISALLNSGAT WCDQPVQAKD IAVITETHKD ARACHAALLA AGIPAVYTGD SDVFTSEAAE DWLYLLEAFD QPHRPGLVRA AAATMFFGET AESLAAGGDA LTDRVADTLR EWAGHARERG VAAIFEAAQL AGMGKRVLSW QGGERLMTDL AHMTQLLQDT AHREGFGLAA LRDWLRTQRS ERGGESERNR RLDSDAAAVQ IMTVWVSKGL QFPVVYLPFA FNRYVPEPDL VLFHDDGQRC LHVGGADPAV ARAGRAEAAG DDSRLTYVAL TRAQSQVVAW WAPSYDEPNG GLSRLMRGRA PGEAIVPDKC SPPKISDEDA LERLRAWEAA GGPVIEESVI GAVSPVPPEP APEDLAARKF FRAIDMAWKR TSYSGLLRAA ETAGVGVSSE PEVTERDDEF DDIPVVAPAE GADVPSPMAH LPTGAAFGSL VHAVLETADP FAEDLTAELA THIDAHSQHW PVEVETAELA AALVPMHDTP LGPLAPGLTL RQIGLRDRLC ELDFEFPMAG GDLRGGRFAR LSDVGELLRE YLPADDPLAV YAERLSTGIL GVQPLRGYLS GSVDAVLRVG EKFVIVDYKT NWLGTGDGTL TAADYGRRRM VEAMLHSDYP LQALLYAVVL HRYLGWRLSG YDPATHLGGV LYLFVRGMCG AGTPVVDGHP AGVFSWEPPA DLVVALSKLL DAEAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaggtt ttcgatctgc tcggcccgct gcccgcaccg aacaccacca cggtgctgga ggccagcgcg ggcaccggca agacgttcgc gctggccggt ctggtcactc gttttgtcgc cgagggcgtc gcgacgctcg accagatgct gctcatcacg ttcggccgcg ccgccagcca ggaactgcgc gagcgtgtgc gcgcccagat cgtcgccgcg ctcgtcgcgt tggacgaccc gtcgcgggcc tgcaacgacc tggaggaata cctggtcaag accgaccagc aggcgcggcg ccggcgcctg cgcgacgcgc tggccgggtt cgacgccgcc accatcgcga ccacgcacca gttctgccag atcgtgctga aatctcttgg cgtggctggg gactccgacg ccggggtgac cctcgtggag agcctcgacg acttggtctc cgagatcgtc gacgatctgt atctcgcgca cttcggcggg cagaaggacg atcccgagct cagctatccc gaggccctca agctcgcgcg cgtcgtcgtc ggcaacccgg ccacccaact gcgcccgcgc gacccggacc ccgattcccc cgccgcggtg cgcctgaagt tcgcgcggga tgtgctcgcc gagttggaga tccgcaagcg tcggcgcggc gtgctcggct acgacgatct gctgacccgg ctggccgatg cactcgaacc cgaggactcc ccggcgcgcg tgcgcatgca gcagcgctgg ccgatcgtga tggtcgacga gttccaggac accgatcccg tgcagtggca ggtgatcgag cgcgcgttct cggggcggtc gacgctggtg ctcatcggtg atcccaagca ggcgatctac gcgttccgcg gtggcgacat cgcgacgtac ctgcgggccg ccgcgacggc cggtgacaag cagacgctgg gcaccaactg gcgcagcgac cgcgcactgg tggaccgcct gcaggccgtg ctgcgcggcg cacaactcgg cggacccgac atcgtggtgc acgacgtgca ggcacgccat caggggcacc gcctggtggg cgcaccgcgc aacgacccgt tccggctgcg cgtggtgtca cgaaaacccg gcaacaccag ggtgatcccg atcgatcagc tgcgccgcca catcggccgg gatctcgcgg ccgacatctc tgccctgctg aacagcgggg ccacgtggtg cgaccaaccg gtccaggcga aggacatcgc ggtcatcacc gagacccaca aggacgccag ggcgtgtcat gcggcgctgc tcgcggcggg cattcccgcg gtctacaccg gggattcgga tgtgttcacc tccgaggccg ccgaggactg gctgtacctg ctcgaggcgt tcgaccagcc gcaccggccg ggtctggtgc gcgccgcggc cgccaccatg ttcttcggcg agaccgcgga aagccttgcc gcgggcggcg atgcgctcac cgaccgggtc gccgacacgc tgcgtgaatg ggcggggcat gcccgtgagc gcggtgtcgc cgcgatcttc gaggccgcgc aactcgccgg tatgggcaag cgtgtgctgt cgtggcaggg cggtgaacgg ttgatgacgg atctcgccca catgacccag ttgctgcagg acaccgcaca ccgcgagggc ttcgggctgg ccgcgctgcg cgactggctg cgcacgcagc gctcggagag gggcggcgaa tcggaacgca accgtcgtct cgacagtgac gccgcggccg tgcagatcat gaccgtgtgg gtgagcaagg gtctgcagtt cccggtcgtc tacctgccgt tcgcgttcaa tcgctatgtg cccgagccgg atctggtgtt gttccacgac gacgggcagc gctgcctgca tgtcggcgga gccgatccgg cggtcgcccg cgcggggcgt gccgaggccg cgggcgacga cagccgtctc acctacgtcg cactgacccg cgcccagtcg caggtggtgg cgtggtgggc accgtcgtac gacgaaccca acggcggcct gtcgcggctg atgcgcggcc gcgctcccgg ggaggcgatc gtccccgaca agtgctcacc acccaagatc tccgatgagg atgcgctgga acggttgcgg gcgtgggagg cagccggtgg gccggtgatc gaggaatcgg tgatcggtgc ggtcagcccg gtgccacccg agcccgcgcc cgaggatctg gcggctcgca agttctttcg cgcgatcgac atggcgtgga agcgcacgtc ctattcgggg ctcttgcgcg cggcagagac cgcgggtgtc ggggtgtcga gcgaacccga ggtcaccgaa cgcgacgacg agttcgacga cattcccgtg gtcgccccgg ccgagggtgc cgacgtgccc tctcccatgg cacacctgcc gacgggcgcg gcgttcggtt cgctcgtgca cgccgtgctg gagaccgccg atccgttcgc cgaggatctg accgccgaac tcgcgacgca catcgacgcc cactcccagc actggccggt cgaggtcgag acggccgaac tcgcggccgc gctcgtgccc atgcacgaca ccccgctggg gccgttggcg ccgggtctca cgctgcggca gatcggtcta cgggaccggt tgtgtgagtt ggatttcgag tttcccatgg ccggtggcga tctgcgtggt ggacggttcg cacggttgtc ggacgtcggt gagctgctgc gtgagtacct gcccgccgac gacccgctgg cggtgtacgc cgagcggttg tccacgggca tcctcggggt gcagccgctg cgcgggtatc tgtcggggtc ggtcgacgcg gtgctgcggg tcggcgagaa gttcgtcatc gtcgactaca agacgaactg gctgggcacc ggcgacggca cgttgaccgc ggccgactac gggcgccgcc gcatggtcga ggccatgttg cattccgact atccgctgca ggccctgctg tatgcggttg tgctgcaccg gtacctgggc tggcggctgt cgggctacga cccggcgacg catctcggtg gtgtgctgta tctgttcgta cgtggcatgt gcggggccgg gacgccggtc gtcgacgggc acccagccgg ggtgttcagc tgggaaccgc ctgccgatct ggtcgtcgca ttgtcgaagc tgttggatgc ggaggcgccg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.17261.a.A2.GE30129
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEDSPARVR MQQRWPIVMV DEFQDTDPVQ WQVIERAFSG RSTLVLIGDP KQAIYAFRGG DIATYLRAAA TAGDKQTLGT NWRSD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga aggttttcga tctgctcggc ccgctgcccg caccgaacac caccacggtg ctggaggcca gcgcgggcac cggcaagacg ttcgcgctgg ccggtctggt cactcgtttt gtcgccgagg gcgtcgcgac gctcgaccag atgctgctca tcacgttcgg ccgcgccgcc agccaggaac tgcgcgagcg tgtgcgcgcc cagatcgtcg ccgcgctcgt cgcgttggac gacccgtcgc gggcctgcaa cgacctggag gaatacctgg tcaagaccga ccagcaggcg cggcgccggc gcctgcgcga cgcgctggcc gggttcgacg ccgccaccat cgcgaccacg caccagttct gccagatcgt gctgaaatct cttggcgtgg ctggggactc cgacgccggg gtgaccctcg tggagagcct cgacgacttg gtctccgaga tcgtcgacga tctgtatctc gcgcacttcg gcgggcagaa ggacgatccc gagctcagct atcccgaggc cctcaagctc gcgcgcgtcg tcgtcggcaa cccggccacc caactgcgcc cgcgcgaccc ggaccccgat tcccccgccg cggtgcgcct gaagttcgcg cgggatgtgc tcgccgagtt ggagatccgc aagcgtcggc gcggcgtgct cggctacgac gatctgctga cccggctggc cgatgcactc gaacccgagg actccccggc gcgcgtgcgc atgcagcagc gctggccgat cgtgatggtc gacgagttcc aggacaccga tcccgtgcag tggcaggtga tcgagcgcgc gttctcgggg cggtcgacgc tggtgctcat cggtgatccc aagcaggcga tctacgcgtt ccgcggtggc gacatcgcga cgtacctgcg ggccgccgcg acggccggtg acaagcagac gctgggcacc aactggcgca gcgactaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEDSPARVR MQQRWPIVMV DEFQDTDPVQ WQVIERAFSG RSTLVLIGDP KQAIYAFRGG DIATYLRAAA TAGDKQTLGT NWRSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaggtt ttcgatctgc tcggcccgct gcccgcaccg aacaccacca cggtgctgga ggccagcgcg ggcaccggca agacgttcgc gctggccggt ctggtcactc gttttgtcgc cgagggcgtc gcgacgctcg accagatgct gctcatcacg ttcggccgcg ccgccagcca ggaactgcgc gagcgtgtgc gcgcccagat cgtcgccgcg ctcgtcgcgt tggacgaccc gtcgcgggcc tgcaacgacc tggaggaata cctggtcaag accgaccagc aggcgcggcg ccggcgcctg cgcgacgcgc tggccgggtt cgacgccgcc accatcgcga ccacgcacca gttctgccag atcgtgctga aatctcttgg cgtggctggg gactccgacg ccggggtgac cctcgtggag agcctcgacg acttggtctc cgagatcgtc gacgatctgt atctcgcgca cttcggcggg cagaaggacg atcccgagct cagctatccc gaggccctca agctcgcgcg cgtcgtcgtc ggcaacccgg ccacccaact gcgcccgcgc gacccggacc ccgattcccc cgccgcggtg cgcctgaagt tcgcgcggga tgtgctcgcc gagttggaga tccgcaagcg tcggcgcggc gtgctcggct acgacgatct gctgacccgg ctggccgatg cactcgaacc cgaggactcc ccggcgcgcg tgcgcatgca gcagcgctgg ccgatcgtga tggtcgacga gttccaggac accgatcccg tgcagtggca ggtgatcgag cgcgcgttct cggggcggtc gacgctggtg ctcatcggtg atcccaagca ggcgatctac gcgttccgcg gtggcgacat cgcgacgtac ctgcgggccg ccgcgacggc cggtgacaag cagacgctgg gcaccaactg gcgcagcgac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.17261.a.A3.GE30130
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQARHQGH RLVGAPRNDP FRLRVVSRKP GNTRVIPIDQ LRRHIGRDLA ADISALLNSG ATWCDQPVQA KDIAVITETH KDARACHAAL LAAGIPAVYT GDSDVFTSEA AEDWLYLLEA FDQPHRPGLV RAAAATMFFG ETAESLAAGG DALTDRVADT LREWAGHARE RGVAAIFEAA QLAGMGKRVL SWQGGERLMT DLAHMTQLLQ DTAHREGFGL AALRDWLRTQ RSERGGESER NRRLDSDAAA VQIMTVWVX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc aggcacgcca tcaggggcac cgcctggtgg gcgcaccgcg caacgacccg ttccggctgc gcgtggtgtc acgaaaaccc ggcaacacca gggtgatccc gatcgatcag ctgcgccgcc acatcggccg ggatctcgcg gccgacatct ctgccctgct gaacagcggg gccacgtggt gcgaccaacc ggtccaggcg aaggacatcg cggtcatcac cgagacccac aaggacgcca gggcgtgtca tgcggcgctg ctcgcggcgg gcattcccgc ggtctacacc ggggattcgg atgtgttcac ctccgaggcc gccgaggact ggctgtacct gctcgaggcg ttcgaccagc cgcaccggcc gggtctggtg cgcgccgcgg ccgccaccat gttcttcggc gagaccgcgg aaagccttgc cgcgggcggc gatgcgctca ccgaccgggt cgccgacacg ctgcgtgaat gggcggggca tgcccgtgag cgcggtgtcg ccgcgatctt cgaggccgcg caactcgccg gtatgggcaa gcgtgtgctg tcgtggcagg gcggtgaacg gttgatgacg gatctcgccc acatgaccca gttgctgcag gacaccgcac accgcgaggg cttcgggctg gccgcgctgc gcgactggct gcgcacgcag cgctcggaga ggggcggcga atcggaacgc aaccgtcgtc tcgacagtga cgccgcggcc gtgcagatca tgaccgtgtg ggtganca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQARHQGH RLVGAPRNDP FRLRVVSRKP GNTRVIPIDQ LRRHIGRDLA ADISALLNSG ATWCDQPVQA KDIAVITETH KDARACHAAL LAAGIPAVYT GDSDVFTSEA AEDWLYLLEA FDQPHRPGLV RAAAATMFFG ETAESLAAGG DALTDRVADT LREWAGHARE RGVAAIFEAA QLAGMGKRVL SWQGGERLMT DLAHMTQLLQ DTAHREGFGL AALRDWLRTQ RSERGGESER NRRLDSDAAA VQIMTVWVSK G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaggca cgccatcagg ggcaccgcct ggtgggcgca ccgcgcaacg acccgttccg gctgcgcgtg gtgtcacgaa aacccggcaa caccagggtg atcccgatcg atcagctgcg ccgccacatc ggccgggatc tcgcggccga catctctgcc ctgctgaaca gcggggccac gtggtgcgac caaccggtcc aggcgaagga catcgcggtc atcaccgaga cccacaagga cgccagggcg tgtcatgcgg cgctgctcgc ggcgggcatt cccgcggtct acaccgggga ttcggatgtg ttcacctccg aggccgccga ggactggctg tacctgctcg aggcgttcga ccagccgcac cggccgggtc tggtgcgcgc cgcggccgcc accatgttct tcggcgagac cgcggaaagc cttgccgcgg gcggcgatgc gctcaccgac cgggtcgccg acacgctgcg tgaatgggcg gggcatgccc gtgagcgcgg tgtcgccgcg atcttcgagg ccgcgcaact cgccggtatg ggcaagcgtg tgctgtcgtg gcagggcggt gaacggttga tgacggatct cgcccacatg acccagttgc tgcaggacac cgcacaccgc gagggcttcg ggctggccgc gctgcgcgac tggctgcgca cgcagcgctc ggagaggggc ggcgaatcgg aacgcaaccg tcgtctcgac agtgacgccg cggccgtgca gatcatgacc gtgtgggtga gcaagggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.17261.a.A4.GE30131
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAARKFF RAIDMAWKRT SYSGLLRAAE TAGVGVSSEP EVTERDDEFD DIPVVAPAEG ADVPSPMAHL PTGAAFGSLV HAVLETADPF AEDLTAELAT HIDAHSQHWP VEVETAELAA ALVPMHDTPL GPLAPGLTLR QIGLRDRLCE LDFEFPMAGG DLRGGRFARL SDVGELLREY LPADDPLAVY AERLSTGILG VQPLRGYLSG SVDAVLRVGE KFVIVDYKTN WLGTGDGTLT AADYGRRRMV EAMLHSDYPL QALLYAVVLH RYLGWRLSGY DPATHLGGVL YLFVRGMCGA GTPVVDGHPA GVFSWEPPAD LVVALSKLLD AE
Validated NT Sequence
atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggcggctc gcaagttctt tcgcgcgatc gacatggcgt ggaagcgcac gtcctattcg gggctcttgc gcgcggcaga gaccgcgggt gtcggggtgt cgagcgaacc cgaggtcacc gaacgcgacg acgagttcga cgacattccc gtggtcgccc cggccgaggg tgccgacgtg ccctctccca tggcacacct gccgacgggc gcggcgttcg gttcgctcgt gcacgccgtg ctggagaccg ccgatccgtt cgccgaggat ctgaccgccg aactcgcgac gcacatcgac gcccactccc agcactggcc ggtcgaggtc gagacggccg aactcgcggc cgcgctcgtg cccatgcacg acaccccgct ggggccgttg gcgccgggtc tcacgctgcg gcagatcggt ctacgggacc ggttgtgtga gttggatttc gagtttccca tggccggtgg cgatctgcgt ggtggacggt tcgcacggtt gtcggacgtc ggtgagctgc tgcgtgagta cctgcccgcc gacgacccgc tggcggtgta cgccgagcgg ttgtccacgg gcatcctcgg ggtgcagccg ctgcgcgggt atctgtcggg gtcggtcgac gcggtgctgc gggtcggcga gaagttcgtc atcgtcgact acaagacgaa ctggctgggc accggcgacg gcacgttgac cgcggccgac tacgggcgcc gccgcatggt cgaggccatg ttgcattccg actatccgct gcaggccctg ctgtatgcgg ttgtgctgca ccggtacctg ggctggcggc tgtcgggcta cgacccggcg acgcatctcg gtggtgtgct gtatctgttc gtacgtggca tgtgcggggc cgggacgccg gtcgtcgacg ggcacccagc cggggtgttc agctgggaac cgcctgccga tctggtcgtc gcattgtcga agctgttgga tgcggagtaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAARKFFRA IDMAWKRTSY SGLLRAAETA GVGVSSEPEV TERDDEFDDI PVVAPAEGAD VPSPMAHLPT GAAFGSLVHA VLETADPFAE DLTAELATHI DAHSQHWPVE VETAELAAAL VPMHDTPLGP LAPGLTLRQI GLRDRLCELD FEFPMAGGDL RGGRFARLSD VGELLREYLP ADDPLAVYAE RLSTGILGVQ PLRGYLSGSV DAVLRVGEKF VIVDYKTNWL GTGDGTLTAA DYGRRRMVEA MLHSDYPLQA LLYAVVLHRY LGWRLSGYDP ATHLGGVLYL FVRGMCGAGT PVVDGHPAGV FSWEPPADLV VALSKLLDAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggctcgc aagttctttc gcgcgatcga catggcgtgg aagcgcacgt cctattcggg gctcttgcgc gcggcagaga ccgcgggtgt cggggtgtcg agcgaacccg aggtcaccga acgcgacgac gagttcgacg acattcccgt ggtcgccccg gccgagggtg ccgacgtgcc ctctcccatg gcacacctgc cgacgggcgc ggcgttcggt tcgctcgtgc acgccgtgct ggagaccgcc gatccgttcg ccgaggatct gaccgccgaa ctcgcgacgc acatcgacgc ccactcccag cactggccgg tcgaggtcga gacggccgaa ctcgcggccg cgctcgtgcc catgcacgac accccgctgg ggccgttggc gccgggtctc acgctgcggc agatcggtct acgggaccgg ttgtgtgagt tggatttcga gtttcccatg gccggtggcg atctgcgtgg tggacggttc gcacggttgt cggacgtcgg tgagctgctg cgtgagtacc tgcccgccga cgacccgctg gcggtgtacg ccgagcggtt gtccacgggc atcctcgggg tgcagccgct gcgcgggtat ctgtcggggt cggtcgacgc ggtgctgcgg gtcggcgaga agttcgtcat cgtcgactac aagacgaact ggctgggcac cggcgacggc acgttgaccg cggccgacta cgggcgccgc cgcatggtcg aggccatgtt gcattccgac tatccgctgc aggccctgct gtatgcggtt gtgctgcacc ggtacctggg ctggcggctg tcgggctacg acccggcgac gcatctcggt ggtgtgctgt atctgttcgt acgtggcatg tgcggggccg ggacgccggt cgtcgacggg cacccagccg gggtgttcag ctgggaaccg cctgccgatc tggtcgtcgc attgtcgaag ctgttggatg cggagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.17261.a.A2.PS00802
PURIFICATION DATe: 9/22/2010
CONCENTRATION: 37.28mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEDSPARVR MQQRWPIVMV DEFQDTDPVQ WQVIERAFSG RSTLVLIGDP KQAIYAFRGG DIATYLRAAA TAGDKQTLGT NWRSD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga aggttttcga tctgctcggc ccgctgcccg caccgaacac caccacggtg ctggaggcca gcgcgggcac cggcaagacg ttcgcgctgg ccggtctggt cactcgtttt gtcgccgagg gcgtcgcgac gctcgaccag atgctgctca tcacgttcgg ccgcgccgcc agccaggaac tgcgcgagcg tgtgcgcgcc cagatcgtcg ccgcgctcgt cgcgttggac gacccgtcgc gggcctgcaa cgacctggag gaatacctgg tcaagaccga ccagcaggcg cggcgccggc gcctgcgcga cgcgctggcc gggttcgacg ccgccaccat cgcgaccacg caccagttct gccagatcgt gctgaaatct cttggcgtgg ctggggactc cgacgccggg gtgaccctcg tggagagcct cgacgacttg gtctccgaga tcgtcgacga tctgtatctc gcgcacttcg gcgggcagaa ggacgatccc gagctcagct atcccgaggc cctcaagctc gcgcgcgtcg tcgtcggcaa cccggccacc caactgcgcc cgcgcgaccc ggaccccgat tcccccgccg cggtgcgcct gaagttcgcg cgggatgtgc tcgccgagtt ggagatccgc aagcgtcggc gcggcgtgct cggctacgac gatctgctga cccggctggc cgatgcactc gaacccgagg actccccggc gcgcgtgcgc atgcagcagc gctggccgat cgtgatggtc gacgagttcc aggacaccga tcccgtgcag tggcaggtga tcgagcgcgc gttctcgggg cggtcgacgc tggtgctcat cggtgatccc aagcaggcga tctacgcgtt ccgcggtggc gacatcgcga cgtacctgcg ggccgccgcg acggccggtg acaagcagac gctgggcacc aactggcgca gcgactaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVFDLLG PLPAPNTTTV LEASAGTGKT FALAGLVTRF VAEGVATLDQ MLLITFGRAA SQELRERVRA QIVAALVALD DPSRACNDLE EYLVKTDQQA RRRRLRDALA GFDAATIATT HQFCQIVLKS LGVAGDSDAG VTLVESLDDL VSEIVDDLYL AHFGGQKDDP ELSYPEALKL ARVVVGNPAT QLRPRDPDPD SPAAVRLKFA RDVLAELEIR KRRRGVLGYD DLLTRLADAL EPEDSPARVR MQQRWPIVMV DEFQDTDPVQ WQVIERAFSG RSTLVLIGDP KQAIYAFRGG DIATYLRAAA TAGDKQTLGT NWRSD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGGTT TTCGATCTGC TCGGCCCGCT GCCCGCACCG AACACCACCA CGGTGCTGGA GGCCAGCGCG GGCACCGGCA AGACGTTCGC GCTGGCCGGT CTGGTCACTC GTTTTGTCGC CGAGGGCGTC GCGACGCTCG ACCAGATGCT GCTCATCACG TTCGGCCGCG CCGCCAGCCA GGAACTGCGC GAGCGTGTGC GCGCCCAGAT CGTCGCCGCG CTCGTCGCGT TGGACGACCC GTCGCGGGCC TGCAACGACC TGGAGGAATA CCTGGTCAAG ACCGACCAGC AGGCGCGGCG CCGGCGCCTG CGCGACGCGC TGGCCGGGTT CGACGCCGCC ACCATCGCGA CCACGCACCA GTTCTGCCAG ATCGTGCTGA AATCTCTTGG CGTGGCTGGG GACTCCGACG CCGGGGTGAC CCTCGTGGAG AGCCTCGACG ACTTGGTCTC CGAGATCGTC GACGATCTGT ATCTCGCGCA CTTCGGCGGG CAGAAGGACG ATCCCGAGCT CAGCTATCCC GAGGCCCTCA AGCTCGCGCG CGTCGTCGTC GGCAACCCGG CCACCCAACT GCGCCCGCGC GACCCGGACC CCGATTCCCC CGCCGCGGTG CGCCTGAAGT TCGCGCGGGA TGTGCTCGCC GAGTTGGAGA TCCGCAAGCG TCGGCGCGGC GTGCTCGGCT ACGACGATCT GCTGACCCGG CTGGCCGATG CACTCGAACC CGAGGACTCC CCGGCGCGCG TGCGCATGCA GCAGCGCTGG CCGATCGTGA TGGTCGACGA GTTCCAGGAC ACCGATCCCG TGCAGTGGCA GGTGATCGAG CGCGCGTTCT CGGGGCGGTC GACGCTGGTG CTCATCGGTG ATCCCAAGCA GGCGATCTAC GCGTTCCGCG GTGGCGACAT CGCGACGTAC CTGCGGGCCG CCGCGACGGC CGGTGACAAG CAGACGCTGG GCACCAACTG GCGCAGCGAC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for MysmA.17261.a.A4.PS00845
PURIFICATION DATe: 11/8/2010
CONCENTRATION: 15.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAARKFF RAIDMAWKRT SYSGLLRAAE TAGVGVSSEP EVTERDDEFD DIPVVAPAEG ADVPSPMAHL PTGAAFGSLV HAVLETADPF AEDLTAELAT HIDAHSQHWP VEVETAELAA ALVPMHDTPL GPLAPGLTLR QIGLRDRLCE LDFEFPMAGG DLRGGRFARL SDVGELLREY LPADDPLAVY AERLSTGILG VQPLRGYLSG SVDAVLRVGE KFVIVDYKTN WLGTGDGTLT AADYGRRRMV EAMLHSDYPL QALLYAVVLH RYLGWRLSGY DPATHLGGVL YLFVRGMCGA GTPVVDGHPA GVFSWEPPAD LVVALSKLLD AE
Validated NT Sequence
atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggcggctc gcaagttctt tcgcgcgatc gacatggcgt ggaagcgcac gtcctattcg gggctcttgc gcgcggcaga gaccgcgggt gtcggggtgt cgagcgaacc cgaggtcacc gaacgcgacg acgagttcga cgacattccc gtggtcgccc cggccgaggg tgccgacgtg ccctctccca tggcacacct gccgacgggc gcggcgttcg gttcgctcgt gcacgccgtg ctggagaccg ccgatccgtt cgccgaggat ctgaccgccg aactcgcgac gcacatcgac gcccactccc agcactggcc ggtcgaggtc gagacggccg aactcgcggc cgcgctcgtg cccatgcacg acaccccgct ggggccgttg gcgccgggtc tcacgctgcg gcagatcggt ctacgggacc ggttgtgtga gttggatttc gagtttccca tggccggtgg cgatctgcgt ggtggacggt tcgcacggtt gtcggacgtc ggtgagctgc tgcgtgagta cctgcccgcc gacgacccgc tggcggtgta cgccgagcgg ttgtccacgg gcatcctcgg ggtgcagccg ctgcgcgggt atctgtcggg gtcggtcgac gcggtgctgc gggtcggcga gaagttcgtc atcgtcgact acaagacgaa ctggctgggc accggcgacg gcacgttgac cgcggccgac tacgggcgcc gccgcatggt cgaggccatg ttgcattccg actatccgct gcaggccctg ctgtatgcgg ttgtgctgca ccggtacctg ggctggcggc tgtcgggcta cgacccggcg acgcatctcg gtggtgtgct gtatctgttc gtacgtggca tgtgcggggc cgggacgccg gtcgtcgacg ggcacccagc cggggtgttc agctgggaac cgcctgccga tctggtcgtc gcattgtcga agctgttgga tgcggagtaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAARKFFRA IDMAWKRTSY SGLLRAAETA GVGVSSEPEV TERDDEFDDI PVVAPAEGAD VPSPMAHLPT GAAFGSLVHA VLETADPFAE DLTAELATHI DAHSQHWPVE VETAELAAAL VPMHDTPLGP LAPGLTLRQI GLRDRLCELD FEFPMAGGDL RGGRFARLSD VGELLREYLP ADDPLAVYAE RLSTGILGVQ PLRGYLSGSV DAVLRVGEKF VIVDYKTNWL GTGDGTLTAA DYGRRRMVEA MLHSDYPLQA LLYAVVLHRY LGWRLSGYDP ATHLGGVLYL FVRGMCGAGT PVVDGHPAGV FSWEPPADLV VALSKLLDAE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCTCGC AAGTTCTTTC GCGCGATCGA CATGGCGTGG AAGCGCACGT CCTATTCGGG GCTCTTGCGC GCGGCAGAGA CCGCGGGTGT CGGGGTGTCG AGCGAACCCG AGGTCACCGA ACGCGACGAC GAGTTCGACG ACATTCCCGT GGTCGCCCCG GCCGAGGGTG CCGACGTGCC CTCTCCCATG GCACACCTGC CGACGGGCGC GGCGTTCGGT TCGCTCGTGC ACGCCGTGCT GGAGACCGCC GATCCGTTCG CCGAGGATCT GACCGCCGAA CTCGCGACGC ACATCGACGC CCACTCCCAG CACTGGCCGG TCGAGGTCGA GACGGCCGAA CTCGCGGCCG CGCTCGTGCC CATGCACGAC ACCCCGCTGG GGCCGTTGGC GCCGGGTCTC ACGCTGCGGC AGATCGGTCT ACGGGACCGG TTGTGTGAGT TGGATTTCGA GTTTCCCATG GCCGGTGGCG ATCTGCGTGG TGGACGGTTC GCACGGTTGT CGGACGTCGG TGAGCTGCTG CGTGAGTACC TGCCCGCCGA CGACCCGCTG GCGGTGTACG CCGAGCGGTT GTCCACGGGC ATCCTCGGGG TGCAGCCGCT GCGCGGGTAT CTGTCGGGGT CGGTCGACGC GGTGCTGCGG GTCGGCGAGA AGTTCGTCAT CGTCGACTAC AAGACGAACT GGCTGGGCAC CGGCGACGGC ACGTTGACCG CGGCCGACTA CGGGCGCCGC CGCATGGTCG AGGCCATGTT GCATTCCGAC TATCCGCTGC AGGCCCTGCT GTATGCGGTT GTGCTGCACC GGTACCTGGG CTGGCGGCTG TCGGGCTACG ACCCGGCGAC GCATCTCGGT GGTGTGCTGT ATCTGTTCGT ACGTGGCATG TGCGGGGCCG GGACGCCGGT CGTCGACGGG CACCCAGCCG GGGTGTTCAG CTGGGAACCG CCTGCCGATC TGGTCGTCGC ATTGTCGAAG CTGTTGGATG CGGAGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA