SSGCID: Target Page

MysmA.17208.a

ECF RNA polymerase sigma factor SigH (ECF sigma factor SigH) (Alternative RNA polymerase sigma factor SigH) (RNA polymerase sigma-H factor) (Sigma-H factor)

CENTER ID:	MysmA.17208.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17208.a.B1.GE40132	full length		1	215

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.1896
RefSeq:	YP_006566641.1
RefSeq:	YP_886280.1
UniProt:	A0QTP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDVDRVEPE TPPEREETDA ELTARFERDA IPLLDQLYGG ALRMTRNPAD AEDLLQETMV KAYAGFRSFR EGTNLKAWLY RILTNTYINS YRKKQRQPSE YPTDEITDWQ LASNAEHSST GLRSAEVEAL EALPDTEIKA ALQALPEEFR MAVYYADVEG FPYKEIAEIM ETPIGTVMSR LHRGRRQLRD LLAGVARDRG FIRGPQLGEP EEVTS

NT Sequence

atgactgacg tcgaccgggt cgagcccgag acgccgccag agcgcgaaga gacagatgcc gagctcaccg ctcggttcga acgcgatgcc atcccgctgc tggatcagtt gtacggcggt gcactgcgga tgacccgtaa cccggcagat gccgaggacc tgctgcagga aacgatggtg aaggcctacg cgggcttccg ttcgttccgc gagggcacca acctcaaggc gtggctctac cggatcctga ccaacaccta catcaacagc taccgcaaga agcagcgcca gccttcggag taccccaccg acgagatcac cgactggcag ctggcgtcca acgccgagca ctcctcgacg ggtctgcgct cggccgaggt cgaggcgctc gaggcgcttc ccgacaccga gatcaaggcc gcgttgcaag ctcttccaga agagttccgg atggcggtgt actacgccga cgtcgaaggg ttcccgtaca aggagatcgc cgagatcatg gaaacgccca tcgggaccgt gatgtcccgt ctgcaccgcg gccgcaggca gctgcgcgat ctgctggccg gtgtggccag ggatcgtggg ttcatccgag gcccgcagct tggtgaaccc gaggaggtga cgtcatga

Details for MysmA.17208.a.B1.GE40132

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/13/2016
EXPECTED MW:	26kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DVDRVEPETP PEREETDAEL TARFERDAIP LLDQLYGGAL RMTRNPADAE DLLQETMVKA YAGFRSFREG TNLKAWLYRI LTNTYINSYR KKQRQPSEYP TDEITDWQLA SNAEHSSTGL RSAEVEALEA LPDTEIKAAL QALPEEFRMA VYYADVEGFP YKEIAEIMET PIGTVMSRLH RGRRQLRDLL AGVARDRGFI RGPQLGXXEE VTS

Validated NT Sequence

atcttactca cttatgacgt cacctcctcg nnntcaccaa gctgcgggcc tcggatgaac ccacgatccc tggccacacc ggccagcaga tcgcgcagct gcctgcggcc gcggtgcaga cgggacatca cggtcccgat gggcgtttcc atgatctcgg cgatctcctt gtacgggaac ccttcgacgt cggcgtagta caccgccatc cggaactctt ctggaagagc ttgcaacgcg gccttgatct cggtgtcggg aagcgcctcg agcgcctcga cctcggccga gcgcagaccc gtcgaggagt gctcggcgtt ggacgccagc tgccagtcgg tgatctcgtc ggtggggtac tccgaaggct ggcgctgctt cttgcggtag ctgttgatgt aggtgttggt caggatccgg tagagccacg ccttgaggtt ggtgccctcg cggaacgaac ggaagcccgc gtaggccttc accatcgttt cctgcagcag gtcctcggca tctgccgggt tacgggtcat ccgcagtgca ccgccgtaca actgatccag cagcgggatg gcatcgcgtt cgaaccgagc ggtgagctcg gcatctgtct cttcgcgctc tggcggcgtc tcgggctcga cccggtcgac gtcagtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg acgtcgaccg ggtcgagccc gagacgccgc cagagcgcga agagacagat gccgagctca ccgctcggtt cgaacgcgat gccatcccgc tgctggatca gttgtacggc ggtgcactgc ggatgacccg taacccggca gatgccgagg acctgctgca ggaaacgatg gtgaaggcct acgcgggctt ccgttcgttc cgcgagggca ccaacctcaa ggcgtggctc taccggatcc tgaccaacac ctacatcaac agctaccgca agaagcagcg ccagccttcg gagtacccca ccgacgagat caccgactgg cagctggcgt ccaacgccga gcactcctcg acgggtctgc gctcggccga ggtcgaggcg ctcgaggcgc ttcccgacac cgagatcaag gccgcgttgc aagctcttcc agaagagttc cggatggcgg tgtactacgc cgacgtcgaa gggttcccgt acaaggagat cgccgagatc atggaaacgc ccatcgggac cgtgatgtcc cgtctgcacc gcggccgcag gcagctgcgc gatctgctgg ccggtgtggc cagggatcgt gggttcatcc gaggcccgca gcttggtgaa cccgaggagg tgacgtcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat